Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 121999062

GI number: 121999062

Start: 2506577

End: 2507380

Strand: Reverse

Name: xthA [H]

Synonym: Hhal_2283

Alternate gene names: 121999062

Gene position: 2507380-2506577 (Counterclockwise)

Preceding gene: 121999074

Following gene: 121999061

Centisome position: 93.61

GC content: 68.66

Gene sequence:

>804_bases
GTGAAGATCGCCTCTTGGAACGTGAACTCGCTGAAGGTCCGCCTGCCCCAGGTCAAGGCGTGGGCGCAGAGCGCGACGCC
GTCGGTCATCGCCCTGCAGGAGACGAAGGTCCCCGACGATCAATTCCCCCTCAAGGCGTTGCAGGAGGCCGGCTATCGGG
CCGCCTTCGCCGGCCAGCGGACCTACAATGGGGTGGCCGTCCTCGCCCGCAGCGCCCCGCAGGACGTGGTGACCGATCTG
CCCGGCTTTGCCGATACCCAGCGTCGGGTGCTCGCCTGCACCGTCTCCGGGGTCCGGGTGATCAACCTCTACGTGCCCAA
CGGCCAGGAGGTGGGGTCCGAGAAGTACGGCTACAAGCTGCGCTGGCTGGAGGCCCTCCACCACTGGGTAGCCCGGGAGC
TGGAGGCCCACGAGCACGTGGTGGTCCTGGGCGACTTCAACATCGCGCCGCAGGCGGCGGACGTCCACGACCCCGCCGAA
TGGGAGGGCAAGGTCCTGTTCAGTCAGTCGGAGCGGGATGCGCTCCGCGGGCTGCTCAATCTGGGACTGGTTGACACCTT
TCGGCTGTTCGAGCAGCCCGAGGGGCAGTTCTCCTGGTGGGACTACCGGGTCAACAGCTTCAAGCGCAACCGCGGCCTGC
GTATCGATCTGATCCTGGCCAGCAGCGCCCTGGCGCGGGCCTGTGTCGCCTCCCGTGTGGATGTCGAGCCGCGCCGCTGG
GAACGCCCGTCGGATCACGCCCCGGTGGTGGCCGAGTTCGACCCCGTGGTGCTGGGGGGTGCCGAGGGCGAGAGGTCGGC
ATGA

Upstream 100 bases:

>100_bases
AGTGTCCCGGCAGCACGGGGCGACGGCAAGCCCCCCGCGGGGTCGTGGCGGCGTCGACAAGCCGTTAAGCTGAGGGCGAC
CGCCGAAGAAGGAGACCGCG

Downstream 100 bases:

>100_bases
AGTACCGCGACCTGCGCGAATTCCTCCACCATCTGGAGGCTCGGGGCGAGCTCAAGCGCATCCGTCACCCGGTGGATCCG
TATCTGGAGATGACGGAGGT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKIASWNVNSLKVRLPQVKAWAQSATPSVIALQETKVPDDQFPLKALQEAGYRAAFAGQRTYNGVAVLARSAPQDVVTDL
PGFADTQRRVLACTVSGVRVINLYVPNGQEVGSEKYGYKLRWLEALHHWVARELEAHEHVVVLGDFNIAPQAADVHDPAE
WEGKVLFSQSERDALRGLLNLGLVDTFRLFEQPEGQFSWWDYRVNSFKRNRGLRIDLILASSALARACVASRVDVEPRRW
ERPSDHAPVVAEFDPVVLGGAEGERSA

Sequences:

>Translated_267_residues
MKIASWNVNSLKVRLPQVKAWAQSATPSVIALQETKVPDDQFPLKALQEAGYRAAFAGQRTYNGVAVLARSAPQDVVTDL
PGFADTQRRVLACTVSGVRVINLYVPNGQEVGSEKYGYKLRWLEALHHWVARELEAHEHVVVLGDFNIAPQAADVHDPAE
WEGKVLFSQSERDALRGLLNLGLVDTFRLFEQPEGQFSWWDYRVNSFKRNRGLRIDLILASSALARACVASRVDVEPRRW
ERPSDHAPVVAEFDPVVLGGAEGERSA
>Mature_267_residues
MKIASWNVNSLKVRLPQVKAWAQSATPSVIALQETKVPDDQFPLKALQEAGYRAAFAGQRTYNGVAVLARSAPQDVVTDL
PGFADTQRRVLACTVSGVRVINLYVPNGQEVGSEKYGYKLRWLEALHHWVARELEAHEHVVVLGDFNIAPQAADVHDPAE
WEGKVLFSQSERDALRGLLNLGLVDTFRLFEQPEGQFSWWDYRVNSFKRNRGLRIDLILASSALARACVASRVDVEPRRW
ERPSDHAPVVAEFDPVVLGGAEGERSA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=28.2945736434109, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=28.2945736434109, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=28.2945736434109, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=40.2255639097744, Blast_Score=182, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI221330655, Length=269, Percent_Identity=31.9702602230483, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17136678, Length=269, Percent_Identity=31.9702602230483, Blast_Score=107, Evalue=9e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29752; Mature: 29752

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIASWNVNSLKVRLPQVKAWAQSATPSVIALQETKVPDDQFPLKALQEAGYRAAFAGQR
CEEECCCCCEEEEECCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEECCCC
TYNGVAVLARSAPQDVVTDLPGFADTQRRVLACTVSGVRVINLYVPNGQEVGSEKYGYKL
CCCCEEEEECCCCHHHHHHCCCCCCCCCEEEEEEECCEEEEEEECCCCHHCCCHHCCEEH
RWLEALHHWVARELEAHEHVVVLGDFNIAPQAADVHDPAEWEGKVLFSQSERDALRGLLN
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
LGLVDTFRLFEQPEGQFSWWDYRVNSFKRNRGLRIDLILASSALARACVASRVDVEPRRW
CCHHHHHHHHCCCCCCEEEEEEEHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCHHHC
ERPSDHAPVVAEFDPVVLGGAEGERSA
CCCCCCCCEEEECCCEEEECCCCCCCC
>Mature Secondary Structure
MKIASWNVNSLKVRLPQVKAWAQSATPSVIALQETKVPDDQFPLKALQEAGYRAAFAGQR
CEEECCCCCEEEEECCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCEEEECCCC
TYNGVAVLARSAPQDVVTDLPGFADTQRRVLACTVSGVRVINLYVPNGQEVGSEKYGYKL
CCCCEEEEECCCCHHHHHHCCCCCCCCCEEEEEEECCEEEEEEECCCCHHCCCHHCCEEH
RWLEALHHWVARELEAHEHVVVLGDFNIAPQAADVHDPAEWEGKVLFSQSERDALRGLLN
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHH
LGLVDTFRLFEQPEGQFSWWDYRVNSFKRNRGLRIDLILASSALARACVASRVDVEPRRW
CCHHHHHHHHCCCCCCEEEEEEEHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCHHHC
ERPSDHAPVVAEFDPVVLGGAEGERSA
CCCCCCCCEEEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]