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The map label for this gene is murE

Identifier: 121998875

GI number: 121998875

Start: 2301223

End: 2302734

Strand: Reverse

Name: murE

Synonym: Hhal_2096

Alternate gene names: 121998875

Gene position: 2302734-2301223 (Counterclockwise)

Preceding gene: 121998876

Following gene: 121998874

Centisome position: 85.97

GC content: 73.28

Gene sequence:

>1512_bases
ATGACTGCTGCGGCCCTCCCCGAAGTGACCCTGCGCTGGCTGCTTGAGCCGTGGGTCGACGAGGCGTTGCCTGCCGCGTC
GATCCAGGGGCTGACCCCCGATACCCGGCGCCTCGAGCCCGGTGCGGTGTTCCTGGCGCTACCCGGTAGCCGGGACCACG
GGCTCGCCTACGTGCCGGCGGCGTTGCAGGCGGGGGCTGCCGCCGTGGTGTGGGATCCGCAGGACGAGGTCGATCCCGGT
GCGGCGAGGACCGCCACCGAGCAGTCCGGTGTGCCGATGGTCGGTGTGCCGGGGCTGCGTCGCCACCTGGGGGCCATCGC
CGGGCGGCTCTACGGCGATCCGTCCCGGGGCATGGAGGTGATTGCGGTTACCGGGACCGACGGCAAGACGTCGGTCAGCC
ATTTCATTGCCCAGCTGCTTAGCGGGCCGGAGGCCCCCTGGGGCGTCGTTGGCACGCTGGGGCACGGCCTGGTGGACGAC
CTGCGCCCGGGCGTGCTGACCACGCCGGATGCCGCGGCACTGCAGAAGGCCCTGGCCGAGTGCCGCGCAGCGGGCGCCAC
CGGGGTGGCGATGGAGGCCTCCTCTCACGCCCTGGAGCAGGGACGCCTGGCCGGTACGGCGGTGGATCTGGCGGTGCTGA
CCCACTTGGGGCGCGACCACCTCGACTATCACGGCTCCGTCGAGGCGTACGCCGACGCCAAGAGCCGCCTGTTCGAGTTC
CCCAGCCTGCGGGCTCAGGTGCTCAATCTAGACGACGCGCTCGGACGGCGCCTGCACGATCGGGCGGCCGGGGCGGCCTT
CACCTACAGTGCCGCCGGGGCGGCCGCTGACCTCGCGGCCGAGGCCGTGATCCCGGGGGCCGACGGCGTGCAGCTGACGC
TCTGTGTTGGCGGGACCCGGCACTCGGTGCGCCTGCCGCTGTTCGGGCATTTCAACGTCGCCAATGTGCTGGCGGCCGTG
GCGGCCGCCCTGGCCTTGGGGCGGCCGATGGATGGGGTGCTGGCCAGGCTGCCGACGCTGCGTCCGGTGCCCGGGCGGAT
GGAGCAGTTCCACGCCGACGGGTCGCCGCTGGTGGTGGTGGATTACGCCCATACGCCGGGCGCGCTGGAGCAGGCGCTGA
CGGCGCTGCGTGCGCACACCACCGGGCGGCTGTGCGTCGTCTTCGGCTGCGGTGGGGAGCGGGATACCGGCAAGCGACCG
CTGATGGGCGAGGTCGCGGCGCGTCTGGCTGATCGCGTGCTGATCACCGACGACAACCCGCGCGGCGAAGACGCTGCGGT
CATCCGCGCTGCCGTCGCCGCCGGCGCCGGGGAGGGCGCCGAGGTGGTGGCAGGGCGTGGCGTCGCCATCGGCGGGGCCA
TCGCCTGGGGTGCCCCGGGCGACGTGGTGTTGATTGCCGGCAAGGGCCACGAGACCGAGCAGGAGATCGACGGGCAGCGG
TTCCCGTTCTCCGACCGTGAGGAGGTGGCGCGGCTGCTCTCCGTGCCCCGGGAGGCGGCATGGATCCGTTGA

Upstream 100 bases:

>100_bases
TCTTCTCCCGGGTGATGGGCAACGCGCTGCGCATGCTCAATGTGCCGCCCGACGATCTCCCGGAGCTTGAAGTGCCGGTC
GTCGCCGAGAGGGAGGGGTC

Downstream 100 bases:

>100_bases
GCCTGGAGCAGATTGCGGCGCTGACCGGTGCCGAGCTGATCGGCAACGGTGGCGGGGCCGTGACGGGGGTGGCGCTGGAC
AGTCGCCGAGCCACTTCGGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTAAALPEVTLRWLLEPWVDEALPAASIQGLTPDTRRLEPGAVFLALPGSRDHGLAYVPAALQAGAAAVVWDPQDEVDPG
AARTATEQSGVPMVGVPGLRRHLGAIAGRLYGDPSRGMEVIAVTGTDGKTSVSHFIAQLLSGPEAPWGVVGTLGHGLVDD
LRPGVLTTPDAAALQKALAECRAAGATGVAMEASSHALEQGRLAGTAVDLAVLTHLGRDHLDYHGSVEAYADAKSRLFEF
PSLRAQVLNLDDALGRRLHDRAAGAAFTYSAAGAAADLAAEAVIPGADGVQLTLCVGGTRHSVRLPLFGHFNVANVLAAV
AAALALGRPMDGVLARLPTLRPVPGRMEQFHADGSPLVVVDYAHTPGALEQALTALRAHTTGRLCVVFGCGGERDTGKRP
LMGEVAARLADRVLITDDNPRGEDAAVIRAAVAAGAGEGAEVVAGRGVAIGGAIAWGAPGDVVLIAGKGHETEQEIDGQR
FPFSDREEVARLLSVPREAAWIR

Sequences:

>Translated_503_residues
MTAAALPEVTLRWLLEPWVDEALPAASIQGLTPDTRRLEPGAVFLALPGSRDHGLAYVPAALQAGAAAVVWDPQDEVDPG
AARTATEQSGVPMVGVPGLRRHLGAIAGRLYGDPSRGMEVIAVTGTDGKTSVSHFIAQLLSGPEAPWGVVGTLGHGLVDD
LRPGVLTTPDAAALQKALAECRAAGATGVAMEASSHALEQGRLAGTAVDLAVLTHLGRDHLDYHGSVEAYADAKSRLFEF
PSLRAQVLNLDDALGRRLHDRAAGAAFTYSAAGAAADLAAEAVIPGADGVQLTLCVGGTRHSVRLPLFGHFNVANVLAAV
AAALALGRPMDGVLARLPTLRPVPGRMEQFHADGSPLVVVDYAHTPGALEQALTALRAHTTGRLCVVFGCGGERDTGKRP
LMGEVAARLADRVLITDDNPRGEDAAVIRAAVAAGAGEGAEVVAGRGVAIGGAIAWGAPGDVVLIAGKGHETEQEIDGQR
FPFSDREEVARLLSVPREAAWIR
>Mature_502_residues
TAAALPEVTLRWLLEPWVDEALPAASIQGLTPDTRRLEPGAVFLALPGSRDHGLAYVPAALQAGAAAVVWDPQDEVDPGA
ARTATEQSGVPMVGVPGLRRHLGAIAGRLYGDPSRGMEVIAVTGTDGKTSVSHFIAQLLSGPEAPWGVVGTLGHGLVDDL
RPGVLTTPDAAALQKALAECRAAGATGVAMEASSHALEQGRLAGTAVDLAVLTHLGRDHLDYHGSVEAYADAKSRLFEFP
SLRAQVLNLDDALGRRLHDRAAGAAFTYSAAGAAADLAAEAVIPGADGVQLTLCVGGTRHSVRLPLFGHFNVANVLAAVA
AALALGRPMDGVLARLPTLRPVPGRMEQFHADGSPLVVVDYAHTPGALEQALTALRAHTTGRLCVVFGCGGERDTGKRPL
MGEVAARLADRVLITDDNPRGEDAAVIRAAVAAGAGEGAEVVAGRGVAIGGAIAWGAPGDVVLIAGKGHETEQEIDGQRF
PFSDREEVARLLSVPREAAWIR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=497, Percent_Identity=41.2474849094567, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1786274, Length=376, Percent_Identity=27.1276595744681, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51943; Mature: 51811

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAALPEVTLRWLLEPWVDEALPAASIQGLTPDTRRLEPGAVFLALPGSRDHGLAYVPA
CCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECH
ALQAGAAAVVWDPQDEVDPGAARTATEQSGVPMVGVPGLRRHLGAIAGRLYGDPSRGMEV
HHHCCCEEEEECCCCCCCCCCCCCCCHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCEEE
IAVTGTDGKTSVSHFIAQLLSGPEAPWGVVGTLGHGLVDDLRPGVLTTPDAAALQKALAE
EEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
CRAAGATGVAMEASSHALEQGRLAGTAVDLAVLTHLGRDHLDYHGSVEAYADAKSRLFEF
HHHCCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHC
PSLRAQVLNLDDALGRRLHDRAAGAAFTYSAAGAAADLAAEAVIPGADGVQLTLCVGGTR
CCHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCC
HSVRLPLFGHFNVANVLAAVAAALALGRPMDGVLARLPTLRPVPGRMEQFHADGSPLVVV
CEEEECEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEE
DYAHTPGALEQALTALRAHTTGRLCVVFGCGGERDTGKRPLMGEVAARLADRVLITDDNP
EECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEECCCC
RGEDAAVIRAAVAAGAGEGAEVVAGRGVAIGGAIAWGAPGDVVLIAGKGHETEQEIDGQR
CCCHHHHHHHHHHHCCCCCCEEEECCCEEECCEEEECCCCCEEEEECCCCCCHHHCCCCC
FPFSDREEVARLLSVPREAAWIR
CCCCCHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TAAALPEVTLRWLLEPWVDEALPAASIQGLTPDTRRLEPGAVFLALPGSRDHGLAYVPA
CCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECH
ALQAGAAAVVWDPQDEVDPGAARTATEQSGVPMVGVPGLRRHLGAIAGRLYGDPSRGMEV
HHHCCCEEEEECCCCCCCCCCCCCCCHHCCCCEECCCHHHHHHHHHHHHHCCCCCCCEEE
IAVTGTDGKTSVSHFIAQLLSGPEAPWGVVGTLGHGLVDDLRPGVLTTPDAAALQKALAE
EEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
CRAAGATGVAMEASSHALEQGRLAGTAVDLAVLTHLGRDHLDYHGSVEAYADAKSRLFEF
HHHCCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHC
PSLRAQVLNLDDALGRRLHDRAAGAAFTYSAAGAAADLAAEAVIPGADGVQLTLCVGGTR
CCHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCC
HSVRLPLFGHFNVANVLAAVAAALALGRPMDGVLARLPTLRPVPGRMEQFHADGSPLVVV
CEEEECEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCEEEE
DYAHTPGALEQALTALRAHTTGRLCVVFGCGGERDTGKRPLMGEVAARLADRVLITDDNP
EECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEECCCC
RGEDAAVIRAAVAAGAGEGAEVVAGRGVAIGGAIAWGAPGDVVLIAGKGHETEQEIDGQR
CCCHHHHHHHHHHHCCCCCCEEEECCCEEECCEEEECCCCCEEEEECCCCCCHHHCCCCC
FPFSDREEVARLLSVPREAAWIR
CCCCCHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA