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The map label for this gene is murF [H]

Identifier: 121998874

GI number: 121998874

Start: 2299883

End: 2301235

Strand: Reverse

Name: murF [H]

Synonym: Hhal_2095

Alternate gene names: 121998874

Gene position: 2301235-2299883 (Counterclockwise)

Preceding gene: 121998875

Following gene: 121998873

Centisome position: 85.92

GC content: 73.24

Gene sequence:

>1353_bases
ATGGATCCGTTGAGCCTGGAGCAGATTGCGGCGCTGACCGGTGCCGAGCTGATCGGCAACGGTGGCGGGGCCGTGACGGG
GGTGGCGCTGGACAGTCGCCGAGCCACTTCGGGGTCGCTCTTCGTGGCCCTGCCGGGGGCGCGCTGCGATGGCCACGACT
TTGCCGATGATGCCGCAGCCCGCGGCGCCGTGGCGGTGCTCGGCACCCGTCCGGTGGGGGCGCTGCCGACGCTGGTGGTC
GAGGACCCGGCGGCGGCCCTCGCGGTCCTGGGTGCGGAGTGCCGGCGGCGCAGCGGGGTGCGTCTGGTGGCCGTGACCGG
CAGCAACGGCAAGACCACGGTCAAGGAACTGCTGGCCGCGATGCTGGCCGAATCCGGGCCAACCCTGGCCACCGAGGGCA
ACCGGAACAACCTGCTGGGGGTCCCGGAGATGCTCTGTCGGCTGGGTGCCGGGCACCGCTTTGCCGTCATCGAGATGGGC
GCCAACGCCCCGGGCGAGATCGCTCAGCTGACCGCCTGGGCGCAGCCCGACGTGGGCGTGGTGACCAATGCCGGCCCGGC
CCACCTGGAGGGGTTCGGCTCGCTGGACGGGGTCGCCCGCGCTAAGGGGGAGCTCTTCCAGGGGCTGCCGCGGCAGGGGG
TGGCCGTCCTGAATGCCGACGACGACTTCTGCGGCTTGTGGCGGGAGATGGCGGGCGCACGGCGGTGCCTGACCTTTGGC
GCCGACGCCGGACAGTTTCGCCATCGGGGGCATGGCAGCCGGCTGGAGCTGGATCTTGGGGGCGGCTGGATGGCGGCGCC
GACCCCGCTGCTCGGGGGCCACAACGCACAGAACATCGCCGCGGCAGCGGCGTGTGCCGCCGCCCTCGAGGTGCCGGCGC
GAACCATCCTCGACCGCATCTCCTCCGCCGCGGCCGTACCGGGGCGGCTGGAGCTGCGTCGCGGAGATCACGGCGGCTGG
TTGGTAGACGACACCTACAATGCCAACCCCGCCAGCCTGCAGGCGGCCATCGATACGGTGACCGGGTTCCAGGCGGCCCC
GTGGCTGCTCCTCGGGGCCATGGGCGAGCTCGGCGCGGAGACTGCGGAGTGGCACAGCCGGGCCGGCCGGCGGGCGCGCG
CCGCCGGGGTGGAGCGGCTGTGGACCCTTGGGGAGGCTGCCGCCCCGGCGGCCGAGGCCTTCGGCGAAGGCGGCCGCTGT
TTTGACAATGCATCGGCGCTGATCGAGGCGTGCCGCGCCGAGTTGCCGGCCGATGCGGTCGTGCTGATCAAGGGGTCGCG
CTCAGCCGCCATGGAGCGCGTGGCGGCGGGCCTGACCTGCACCGAACCAACAAGAGAAGGGGAGGGACGCTAG

Upstream 100 bases:

>100_bases
GCAAGGGCCACGAGACCGAGCAGGAGATCGACGGGCAGCGGTTCCCGTTCTCCGACCGTGAGGAGGTGGCGCGGCTGCTC
TCCGTGCCCCGGGAGGCGGC

Downstream 100 bases:

>100_bases
GTGCTCTACCACTTGGCCATGGTACTGGAGACGTTCTACTCCGGGTTCAACGTCTTCCAGTACATCACGTTCCGGACCAT
CCTCGGGGTGCTGACCGCCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDPLSLEQIAALTGAELIGNGGGAVTGVALDSRRATSGSLFVALPGARCDGHDFADDAAARGAVAVLGTRPVGALPTLVV
EDPAAALAVLGAECRRRSGVRLVAVTGSNGKTTVKELLAAMLAESGPTLATEGNRNNLLGVPEMLCRLGAGHRFAVIEMG
ANAPGEIAQLTAWAQPDVGVVTNAGPAHLEGFGSLDGVARAKGELFQGLPRQGVAVLNADDDFCGLWREMAGARRCLTFG
ADAGQFRHRGHGSRLELDLGGGWMAAPTPLLGGHNAQNIAAAAACAAALEVPARTILDRISSAAAVPGRLELRRGDHGGW
LVDDTYNANPASLQAAIDTVTGFQAAPWLLLGAMGELGAETAEWHSRAGRRARAAGVERLWTLGEAAAPAAEAFGEGGRC
FDNASALIEACRAELPADAVVLIKGSRSAAMERVAAGLTCTEPTREGEGR

Sequences:

>Translated_450_residues
MDPLSLEQIAALTGAELIGNGGGAVTGVALDSRRATSGSLFVALPGARCDGHDFADDAAARGAVAVLGTRPVGALPTLVV
EDPAAALAVLGAECRRRSGVRLVAVTGSNGKTTVKELLAAMLAESGPTLATEGNRNNLLGVPEMLCRLGAGHRFAVIEMG
ANAPGEIAQLTAWAQPDVGVVTNAGPAHLEGFGSLDGVARAKGELFQGLPRQGVAVLNADDDFCGLWREMAGARRCLTFG
ADAGQFRHRGHGSRLELDLGGGWMAAPTPLLGGHNAQNIAAAAACAAALEVPARTILDRISSAAAVPGRLELRRGDHGGW
LVDDTYNANPASLQAAIDTVTGFQAAPWLLLGAMGELGAETAEWHSRAGRRARAAGVERLWTLGEAAAPAAEAFGEGGRC
FDNASALIEACRAELPADAVVLIKGSRSAAMERVAAGLTCTEPTREGEGR
>Mature_450_residues
MDPLSLEQIAALTGAELIGNGGGAVTGVALDSRRATSGSLFVALPGARCDGHDFADDAAARGAVAVLGTRPVGALPTLVV
EDPAAALAVLGAECRRRSGVRLVAVTGSNGKTTVKELLAAMLAESGPTLATEGNRNNLLGVPEMLCRLGAGHRFAVIEMG
ANAPGEIAQLTAWAQPDVGVVTNAGPAHLEGFGSLDGVARAKGELFQGLPRQGVAVLNADDDFCGLWREMAGARRCLTFG
ADAGQFRHRGHGSRLELDLGGGWMAAPTPLLGGHNAQNIAAAAACAAALEVPARTILDRISSAAAVPGRLELRRGDHGGW
LVDDTYNANPASLQAAIDTVTGFQAAPWLLLGAMGELGAETAEWHSRAGRRARAAGVERLWTLGEAAAPAAEAFGEGGRC
FDNASALIEACRAELPADAVVLIKGSRSAAMERVAAGLTCTEPTREGEGR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=448, Percent_Identity=40.4017857142857, Blast_Score=273, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 45973; Mature: 45973

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLSLEQIAALTGAELIGNGGGAVTGVALDSRRATSGSLFVALPGARCDGHDFADDAAA
CCCCCHHHHHHHHCHHHEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHH
RGAVAVLGTRPVGALPTLVVEDPAAALAVLGAECRRRSGVRLVAVTGSNGKTTVKELLAA
CCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
MLAESGPTLATEGNRNNLLGVPEMLCRLGAGHRFAVIEMGANAPGEIAQLTAWAQPDVGV
HHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHEEECCCCCCCE
VTNAGPAHLEGFGSLDGVARAKGELFQGLPRQGVAVLNADDDFCGLWREMAGARRCLTFG
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHEEEEEC
ADAGQFRHRGHGSRLELDLGGGWMAAPTPLLGGHNAQNIAAAAACAAALEVPARTILDRI
CCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSAAAVPGRLELRRGDHGGWLVDDTYNANPASLQAAIDTVTGFQAAPWLLLGAMGELGAE
HHHHCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
TAEWHSRAGRRARAAGVERLWTLGEAAAPAAEAFGEGGRCFDNASALIEACRAELPADAV
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEE
VLIKGSRSAAMERVAAGLTCTEPTREGEGR
EEEECCHHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure
MDPLSLEQIAALTGAELIGNGGGAVTGVALDSRRATSGSLFVALPGARCDGHDFADDAAA
CCCCCHHHHHHHHCHHHEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHH
RGAVAVLGTRPVGALPTLVVEDPAAALAVLGAECRRRSGVRLVAVTGSNGKTTVKELLAA
CCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
MLAESGPTLATEGNRNNLLGVPEMLCRLGAGHRFAVIEMGANAPGEIAQLTAWAQPDVGV
HHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHEEECCCCCCCE
VTNAGPAHLEGFGSLDGVARAKGELFQGLPRQGVAVLNADDDFCGLWREMAGARRCLTFG
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHEEEEEC
ADAGQFRHRGHGSRLELDLGGGWMAAPTPLLGGHNAQNIAAAAACAAALEVPARTILDRI
CCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSAAAVPGRLELRRGDHGGWLVDDTYNANPASLQAAIDTVTGFQAAPWLLLGAMGELGAE
HHHHCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
TAEWHSRAGRRARAAGVERLWTLGEAAAPAAEAFGEGGRCFDNASALIEACRAELPADAV
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEE
VLIKGSRSAAMERVAAGLTCTEPTREGEGR
EEEECCHHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]