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The map label for this gene is yjeA [H]

Identifier: 121998757

GI number: 121998757

Start: 2180091

End: 2181041

Strand: Reverse

Name: yjeA [H]

Synonym: Hhal_1978

Alternate gene names: 121998757

Gene position: 2181041-2180091 (Counterclockwise)

Preceding gene: 121998758

Following gene: 121998752

Centisome position: 81.43

GC content: 73.71

Gene sequence:

>951_bases
ATGGCGGCCGGCGGTCCGTGCGGCGCCGCCGACGGCGCCACCGTGCGCCGGGTCGGTCAGAAGCTGGCACGGATCCGCGG
CTTCTTCGCCGAGCGCGGGGTGGTGGAGGTGCAGCCGCCGTGCCTCGGCCCGGCGGCCATGGACCCGGCGCTGGAGAGCA
TCCCGGTGCCCGGGGCGGCCGCCGACGGCGACGCCGCCTACCTGCAGACCTCCCCGGAATCGGCGATGAAGGCTATGCTC
GCCGCCGGCAGCAGCGACATCTACTACCTCGGGCCGGCCTGGCGGGGCGGTGAGCGCGGGCGCTGGCACGCCACCGAGTT
CACGCTGCTCGAGTGGTACCGCATCGGCTTCGACCACCACCGGCTGATGGACGAGGTGTCGGCGCTGGCGGCGCTGCTTG
TCGGTGATCGCCCGGTCGAGCGCTGGTCCTACCAAGAGGCCTTTCGCCGTTACGCCGGTGTTGATCCCTTGGACGACGCG
GATGACGCACTCCGCGACGCTCTGGCGGACCAGGGCATCGATCTGCACGGGGCCGCCCCGAGCCGCGATGACGCGCTCGA
TCTGCTGCTCTCCCACGCCGTGGGGCCGGCCCTGGGCCACGGCCAGTTGACCTTTCTCTACGGGTTTCCGGCGAGCCAGG
CGGCCCTGGCCCGCCTTGACGACGCTGACCCGCGCACTGCGCGGCGTTTCGAGTGCTTCATCGACGGCCTGGAGATCGCC
AACGGCTACCACGAACTCACCGACCCCGCCGCGCAGCGGGCGCGCTTCGAGGCCGAGCAGGCCGAGCGCCGCGGCGCCGG
GCAGCCGGTGTACGACCTCGACGAGCGGCTGCTCGGCGCCCTGGAGCGCGGTGTACCCGACTGCGCCGGGGTGGCGCTGG
GCGTCGACCGGCTGCTGGCGGTGGCGCTGGGGGCAGAGTCGTTGGCGGCGGTGCGGGGTTCGGGCGGCTAA

Upstream 100 bases:

>100_bases
AGACCGGCGCCACGGTGCGCGTGCCCCTGTTCATCGAGGAAGGCGAGGTGCTGCGCGTCGATACCCGCAAGGCGGAGTAC
GTCAGCCGCGCCAAGGACTG

Downstream 100 bases:

>100_bases
GCCGCGCACTCGGCACATCTTGTCAGCGTGGGGTCGGCCTGGAGGCGGCGCGGGTCGATGTCCTCGCCGCAGATGAAGCA
GTAGCCGAAGTCGCCGCTGT

Product: lysine--tRNA ligase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAAGGPCGAADGATVRRVGQKLARIRGFFAERGVVEVQPPCLGPAAMDPALESIPVPGAAADGDAAYLQTSPESAMKAML
AAGSSDIYYLGPAWRGGERGRWHATEFTLLEWYRIGFDHHRLMDEVSALAALLVGDRPVERWSYQEAFRRYAGVDPLDDA
DDALRDALADQGIDLHGAAPSRDDALDLLLSHAVGPALGHGQLTFLYGFPASQAALARLDDADPRTARRFECFIDGLEIA
NGYHELTDPAAQRARFEAEQAERRGAGQPVYDLDERLLGALERGVPDCAGVALGVDRLLAVALGAESLAAVRGSGG

Sequences:

>Translated_316_residues
MAAGGPCGAADGATVRRVGQKLARIRGFFAERGVVEVQPPCLGPAAMDPALESIPVPGAAADGDAAYLQTSPESAMKAML
AAGSSDIYYLGPAWRGGERGRWHATEFTLLEWYRIGFDHHRLMDEVSALAALLVGDRPVERWSYQEAFRRYAGVDPLDDA
DDALRDALADQGIDLHGAAPSRDDALDLLLSHAVGPALGHGQLTFLYGFPASQAALARLDDADPRTARRFECFIDGLEIA
NGYHELTDPAAQRARFEAEQAERRGAGQPVYDLDERLLGALERGVPDCAGVALGVDRLLAVALGAESLAAVRGSGG
>Mature_315_residues
AAGGPCGAADGATVRRVGQKLARIRGFFAERGVVEVQPPCLGPAAMDPALESIPVPGAAADGDAAYLQTSPESAMKAMLA
AGSSDIYYLGPAWRGGERGRWHATEFTLLEWYRIGFDHHRLMDEVSALAALLVGDRPVERWSYQEAFRRYAGVDPLDDAD
DALRDALADQGIDLHGAAPSRDDALDLLLSHAVGPALGHGQLTFLYGFPASQAALARLDDADPRTARRFECFIDGLEIAN
GYHELTDPAAQRARFEAEQAERRGAGQPVYDLDERLLGALERGVPDCAGVALGVDRLLAVALGAESLAAVRGSGG

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=297, Percent_Identity=44.4444444444444, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1790571, Length=318, Percent_Identity=28.6163522012579, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789256, Length=321, Percent_Identity=25.8566978193146, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71994340, Length=338, Percent_Identity=25.7396449704142, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=338, Percent_Identity=25.7396449704142, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=338, Percent_Identity=25.7396449704142, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=322, Percent_Identity=27.3291925465839, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24640851, Length=321, Percent_Identity=22.7414330218069, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640849, Length=321, Percent_Identity=22.7414330218069, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 33519; Mature: 33388

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGGPCGAADGATVRRVGQKLARIRGFFAERGVVEVQPPCLGPAAMDPALESIPVPGAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHCCHHHHCCCCCCC
ADGDAAYLQTSPESAMKAMLAAGSSDIYYLGPAWRGGERGRWHATEFTLLEWYRIGFDHH
CCCCCEEEECCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHCCHHH
RLMDEVSALAALLVGDRPVERWSYQEAFRRYAGVDPLDDADDALRDALADQGIDLHGAAP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
SRDDALDLLLSHAVGPALGHGQLTFLYGFPASQAALARLDDADPRTARRFECFIDGLEIA
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NGYHELTDPAAQRARFEAEQAERRGAGQPVYDLDERLLGALERGVPDCAGVALGVDRLLA
CCHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VALGAESLAAVRGSGG
HHHCHHHHHHHCCCCC
>Mature Secondary Structure 
AAGGPCGAADGATVRRVGQKLARIRGFFAERGVVEVQPPCLGPAAMDPALESIPVPGAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHCCHHHHCCCCCCC
ADGDAAYLQTSPESAMKAMLAAGSSDIYYLGPAWRGGERGRWHATEFTLLEWYRIGFDHH
CCCCCEEEECCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHHCCHHH
RLMDEVSALAALLVGDRPVERWSYQEAFRRYAGVDPLDDADDALRDALADQGIDLHGAAP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
SRDDALDLLLSHAVGPALGHGQLTFLYGFPASQAALARLDDADPRTARRFECFIDGLEIA
CCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NGYHELTDPAAQRARFEAEQAERRGAGQPVYDLDERLLGALERGVPDCAGVALGVDRLLA
CCHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VALGAESLAAVRGSGG
HHHCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]