Click here to switch to the map view.

The map label for this gene is 121998503

Identifier: 121998503

GI number: 121998503

Start: 1890721

End: 1892088

Strand: Reverse

Name: 121998503

Synonym: Hhal_1724

Alternate gene names: NA

Gene position: 1892088-1890721 (Counterclockwise)

Preceding gene: 121998504

Following gene: 121998502

Centisome position: 70.64

GC content: 66.96

Gene sequence:

>1368_bases
ATGTCAGGAGTCCCCAGCCAACCCGGTGAGCCGGTGACCGATGTCGCGCAGCTGGCAGAGCATCTTGAGCGCGGTTCCCG
GCCCCCGGAGCAGTGGCGGATCGGCACCGAACATGAGAAGTTCGTCCACCATCTGGAAGACTTCAGCCCGGTGCCGTACG
CGGGGGAGCGCGGCATCGGCGCCTTCCTCGAAGGGCTGGTGCGCTTCGGCTGGCAGCCGGTGCACGAGGATGGACGGGTG
ATCGCCCTCAAGCGCGAGGGCGGTGCCTCGGTCAGCCTTGAGCCCGGCGGACAGGTGGAGCTCTCCGGGGCGCAGCTCGA
GACCCTGCACGAGGCCTGTGTGGAGGTCCGGGAGCATCTGCAGCAGGTGGACACCGTGGCCCGCGAGCTGCAGATCGGGT
TCTCAGGGTTCGGTTTTCACCCGGTTTGGCGGCGCGAGCAGATCCCCTGGATGCCCAAGGCGCGTTACGGCGTGATGGGC
CGCTACATGCCCAAGGTGGGTCACCTGGGGCTGGATATGATGCTGCGCACCTGCACGGTACAGGTGAATCTGGACTTCTC
CAGTGAGGCGGACATGGTCCGCAAGTTCCGGGTCGGCCTGGCCCTGCAGCCGATCGCCACTGCGCTGTTTGCCAACTCGC
CGTTCACCAATGGCCAGCCCAACGGCTATCTCAGCTACCGCAGCCGGGTGTGGGAAGACACCGACCCCGATCGCTGCGGC
ATGCTCGACTTCGTCTTCGAGTCCGGTATGGGGTTCGAGCGCTATGTGGACTACGTGCTCGATGTACCCATGTATTTCGT
CTATCGCGATGGCCAGTACATCGACTGCGCCGGCGAGTCGTTCCGCGATTTCATGGCGGGGCGACTGCCGCAGTTGCCGG
GAGAGCGGCCGACACTCAGTGACTGGGACGACCATCTGACGACGCTCTTCCCCGAGGTGCGGCTCAAGCAGTTTCTGGAG
ATGCGCGGGGCGGACGGTGGCCCGTGGCGGGGGTTGTGCGCGCTGCCGGCACTGTGGACGGGGCTGCTCTACGATGACGC
GGCCCTGGCTGCGGCCGAGGCGTTGATTCGTGACTGGAGTGTTGAGGAGATGCGCGCCCTGCGCCACGAGGTCCCGCGAA
CGGCGCTGCACACCCCGTTCCGCGAGACGACTGTGGGGGCATTGGCCCGGGAGGTGCTCACCATCGCCAGCGACGGACTG
CGCAACCGCCAGCGTCTGGATCAGCGGGGGCGGGATGAGCGCCGCCACCTGGAGCGGCTCTGGGAGATCGTCGATGCAGG
GCGGACCCCCGCCGAGGACCTGCTGAGTGCCTACCACGGGCGCTGGGGCGGATCGCTGGATCCGATCTTCACGGAGTACG
CCTACTAG

Upstream 100 bases:

>100_bases
CAGCGCAACCGCCGTCCGGCGAGGAAGCGGCTTTCGTCGAAACCGTCGACGCGGACGGGTAAGATAGCCGGCCTTGCCTT
GTGCCGGATCGAGGAGAGCT

Downstream 100 bases:

>100_bases
CAGGCTGCTGAAAACCCTTTTCAGCAGCCTGCTTTCACCGCTGACGCGGTGGACCAGGATGAAGCTTTTCCTGGCAAGCC
TGGAAAAGCAGTGAGTTCGG

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSGVPSQPGEPVTDVAQLAEHLERGSRPPEQWRIGTEHEKFVHHLEDFSPVPYAGERGIGAFLEGLVRFGWQPVHEDGRV
IALKREGGASVSLEPGGQVELSGAQLETLHEACVEVREHLQQVDTVARELQIGFSGFGFHPVWRREQIPWMPKARYGVMG
RYMPKVGHLGLDMMLRTCTVQVNLDFSSEADMVRKFRVGLALQPIATALFANSPFTNGQPNGYLSYRSRVWEDTDPDRCG
MLDFVFESGMGFERYVDYVLDVPMYFVYRDGQYIDCAGESFRDFMAGRLPQLPGERPTLSDWDDHLTTLFPEVRLKQFLE
MRGADGGPWRGLCALPALWTGLLYDDAALAAAEALIRDWSVEEMRALRHEVPRTALHTPFRETTVGALAREVLTIASDGL
RNRQRLDQRGRDERRHLERLWEIVDAGRTPAEDLLSAYHGRWGGSLDPIFTEYAY

Sequences:

>Translated_455_residues
MSGVPSQPGEPVTDVAQLAEHLERGSRPPEQWRIGTEHEKFVHHLEDFSPVPYAGERGIGAFLEGLVRFGWQPVHEDGRV
IALKREGGASVSLEPGGQVELSGAQLETLHEACVEVREHLQQVDTVARELQIGFSGFGFHPVWRREQIPWMPKARYGVMG
RYMPKVGHLGLDMMLRTCTVQVNLDFSSEADMVRKFRVGLALQPIATALFANSPFTNGQPNGYLSYRSRVWEDTDPDRCG
MLDFVFESGMGFERYVDYVLDVPMYFVYRDGQYIDCAGESFRDFMAGRLPQLPGERPTLSDWDDHLTTLFPEVRLKQFLE
MRGADGGPWRGLCALPALWTGLLYDDAALAAAEALIRDWSVEEMRALRHEVPRTALHTPFRETTVGALAREVLTIASDGL
RNRQRLDQRGRDERRHLERLWEIVDAGRTPAEDLLSAYHGRWGGSLDPIFTEYAY
>Mature_454_residues
SGVPSQPGEPVTDVAQLAEHLERGSRPPEQWRIGTEHEKFVHHLEDFSPVPYAGERGIGAFLEGLVRFGWQPVHEDGRVI
ALKREGGASVSLEPGGQVELSGAQLETLHEACVEVREHLQQVDTVARELQIGFSGFGFHPVWRREQIPWMPKARYGVMGR
YMPKVGHLGLDMMLRTCTVQVNLDFSSEADMVRKFRVGLALQPIATALFANSPFTNGQPNGYLSYRSRVWEDTDPDRCGM
LDFVFESGMGFERYVDYVLDVPMYFVYRDGQYIDCAGESFRDFMAGRLPQLPGERPTLSDWDDHLTTLFPEVRLKQFLEM
RGADGGPWRGLCALPALWTGLLYDDAALAAAEALIRDWSVEEMRALRHEVPRTALHTPFRETTVGALAREVLTIASDGLR
NRQRLDQRGRDERRHLERLWEIVDAGRTPAEDLLSAYHGRWGGSLDPIFTEYAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51505; Mature: 51374

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVPSQPGEPVTDVAQLAEHLERGSRPPEQWRIGTEHEKFVHHLEDFSPVPYAGERGIG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
AFLEGLVRFGWQPVHEDGRVIALKREGGASVSLEPGGQVELSGAQLETLHEACVEVREHL
HHHHHHHHCCCCEECCCCCEEEEEECCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHH
QQVDTVARELQIGFSGFGFHPVWRREQIPWMPKARYGVMGRYMPKVGHLGLDMMLRTCTV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
QVNLDFSSEADMVRKFRVGLALQPIATALFANSPFTNGQPNGYLSYRSRVWEDTDPDRCG
EEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
MLDFVFESGMGFERYVDYVLDVPMYFVYRDGQYIDCAGESFRDFMAGRLPQLPGERPTLS
HHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCEEECCCHHHHHHHHCCCCCCCCCCCCCC
DWDDHLTTLFPEVRLKQFLEMRGADGGPWRGLCALPALWTGLLYDDAALAAAEALIRDWS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEEMRALRHEVPRTALHTPFRETTVGALAREVLTIASDGLRNRQRLDQRGRDERRHLERL
HHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHH
WEIVDAGRTPAEDLLSAYHGRWGGSLDPIFTEYAY
HHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
SGVPSQPGEPVTDVAQLAEHLERGSRPPEQWRIGTEHEKFVHHLEDFSPVPYAGERGIG
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
AFLEGLVRFGWQPVHEDGRVIALKREGGASVSLEPGGQVELSGAQLETLHEACVEVREHL
HHHHHHHHCCCCEECCCCCEEEEEECCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHH
QQVDTVARELQIGFSGFGFHPVWRREQIPWMPKARYGVMGRYMPKVGHLGLDMMLRTCTV
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
QVNLDFSSEADMVRKFRVGLALQPIATALFANSPFTNGQPNGYLSYRSRVWEDTDPDRCG
EEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
MLDFVFESGMGFERYVDYVLDVPMYFVYRDGQYIDCAGESFRDFMAGRLPQLPGERPTLS
HHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCEEECCCHHHHHHHHCCCCCCCCCCCCCC
DWDDHLTTLFPEVRLKQFLEMRGADGGPWRGLCALPALWTGLLYDDAALAAAEALIRDWS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEEMRALRHEVPRTALHTPFRETTVGALAREVLTIASDGLRNRQRLDQRGRDERRHLERL
HHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHH
WEIVDAGRTPAEDLLSAYHGRWGGSLDPIFTEYAY
HHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA