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The map label for this gene is cheR [C]

Identifier: 121998495

GI number: 121998495

Start: 1879790

End: 1882360

Strand: Reverse

Name: cheR [C]

Synonym: Hhal_1716

Alternate gene names: 121998495

Gene position: 1882360-1879790 (Counterclockwise)

Preceding gene: 121998498

Following gene: 121998494

Centisome position: 70.28

GC content: 64.96

Gene sequence:

>2571_bases
ATGCCCCCACCGACTGACCGCCGCCCACTGGACCCCTTACCGATCCCGATCGTAGCGATCGGCGGGTCGGCTGGATCCCT
CAAGCCCCTGCAGGCTTTCCTGGCCCACCTGCCGACGGACTCGGGGGCAGCCTACGTCATCATCACTCACCACCCCAGCG
GTCAGCGAAGCCTGCTCCCCGAGATCTTGGAGCGGCAGACGGAGCTGCCCGTGCAGATGGTCGAAGAGGGGGAGACCCTG
GGCCCGGACCGCATCTACATTGCCCTGCCGGAGGACGGCTGGGCCCTCCGGCAGGGGCAGCTGATCCGGTCGCGAGCAAC
GGAAACGCGCCCTGGAGGTGGCGCCCCCTATCCCATTGATGCCTTTTTCCGCTCCCTCGCCGAGGAGGCGGGACAGGGTG
GACTGGCCGTTGTCCTGTCCGGCACGGGTAGTGACGGCACCCTTGGGGTCAAGGCCATCAAGTCCCAGTCGGGCATGGTT
ATGGCTCAGGATCCGAAGACCGCTGAGTTCGGCGACATGCCGGCCAATGCCATCGCCACGGGGCAGGTGGACTACGTGCT
AGCCCCGGCGGATATGCCTGCGACCCTGGTCGGTTATCTCGGGGCCCGCAGCGAGCGCCAGGGTGCGCCGTCGAGTCGTC
GCCGCACGATCCCCGAAGAGGTTCTGGGCCCGATCCTTCGACAAGTCAGGCACTGCACCGGTCACGATTTCTCGGGTTAC
AAGACCAGCACGCTCGAGCGGCGCCTGGAGCGGCGCATGGACCTCTTGCAGATTTCCGATCCGCGCGCCTACTCGATCCA
CCTGCAGTCCCATCCCGAGGAACTCAACCGCCTGTTCCAGGATTTCCTGATCAGTGTCACCAATTTCTTCCGGGATCCCC
GGGCCTGGGCCACCCTGTCGGAGGAACTGCTACCAGGCATGCTGCGGCGAGCCGCGCACAGGGGGGGCGAGTTCCGGGCC
TGGGTGGTGGGCTGTGCCACCGGCGAGGAGGCCTACACCCTGGCCATTCTCATCCGGGAATGCCTAGCGGATTGGGAACA
GGCCCCGGAGGTGCGGATCTTCGCCACCGATGTCGATCAGGCTGCCATCGAGACGGCCCGAGTGGGGCGTTATCCGGCCG
GCATTGCCGACGACCTCACCCACCAGCGTTTGCGGAGGTATTTCGCCGCTGAGAACGATACCTACCGGATCGTCCGGGAA
GTGCGGGACATGGTGATCTTTGCCGACCACAATGCGCTGCAGGATCCGCCGTTCACCCACATGGATCTGATAACCTGCCG
TAACCTTCTGATTTACCTGGAACGGGACCATCAGATGCGCCTCCTGGCCCTGTTCCGCTACGCCCTGCGTGACCGCGGGC
TCCTCTTCCTGGGGCCTTCCGAGAGTCCGGATCAGGCGAGCGAAGCCTTCGAGGTTGAGGCCAAAGAGGATCGGATCTAC
CGGGTGGCCGAAGATGCGGCACGGACCAACCTGCTTGACATGCCCGGACCCCGCCGCTTTGCGCGCAGCCTGCCGGTGCA
GAGTACAGCCGCGCCCGCGGCGAACAGTGACGGGGAGGGTTTCTCCCACCACGTGGAACGTCTCCTTGCCCTGCGGTTTG
CGCCACCGTCAGTGGTGGTCAACGACCGCGGCGAGGTGGCGTACATCCACGGGCGCACCGGTCGCTTCCTGGAGCCCGCT
CACGGCCCGGTCCAGAACCAACTGGTGGAGATGGCGCGGCCCGGGCTGCGGGCTCCGCTGAGCCAAGCCCTGGAGGAGGT
GGCGCGTGGCCGGGCAGAAGAGGTGGGCCAGAGGGTTCAGATCCAGACCGACGATGATGCCGATCCGGAGTGGCTCCGGT
TTGAAGTCCGCCGCATCCACACGCCTGCCGCCTTGAAGGGGTTTCGCCTCGTGACCTTGTATTCGGAGGCGGGTACGGAG
GTGGCGTCCCTTGGGGTGGAGCTTGAGCCGAGATCCGAGTCGGAGGCGGAGTCCGAGGGTCCAGGGACTGAGGCCGAGCG
GCGTTTGAGGCGAGAGCTGGAAAACGCCCGCCAGGATAAGCAGGTAGCGGTCAATGAGATGCAGTCTACCAATGAAGAGC
TCCAATCCATGAATGAGGAGCTTCAGTCGATGAATGAGGAGCTCCAGAGCAGCAACGAGGAACTGGAGGTCGCCAAGGAG
GAGGTGGAGTCGCTCAACCAAGAGCTGCGCAGCGTCAACGCCGAACTCCAGGTCCGGGTGGACGACCTGACCGAGACCAA
CGACGACCTCAAGAACCTTCTGGACAGCACGCACATCGCTACGCTGTTTCTGGACGAAGCCCTGAATATCAAACGCTTTA
CGACCCCTGTGCAGGAGCTGATCGCCGTGCGCCCGGCGGACATCGGCCGGCCGGTGGCCGATCTGAAGACCAGCTTGCGG
TATGACGGCCTAGTGGCGGATGCCGAGTCCGTTCTCGACACCCTGATCCCCCACGAGGTGCAGGTGCAGTCCGATGGCGG
CGATTGGTATCTGCTGCGCATCCAGCCCTACCGCAGTACGCGGAACGTGATCCGCGGGGTGGTCTGCACCTTCCAGTCTG
CTTGCACCTGA

Upstream 100 bases:

>100_bases
CCCATCCTTAGCGTGGGCCGTCGCGGATATTGCCATGAGGCCGCAATTATGCGGGTATGGCAAGGCTAGAGACCGTTGAC
CGAGGATCCAGGATCCGCCC

Downstream 100 bases:

>100_bases
GTACCCGGCGCCTAGACTCGAAAGACAGTGTGGCTCTGATCCCGCTCGACATGGATGTCGCGTAACGGAAGTCCTGACAA
TGAGATGGCCTTAAAGGAAC

Product: MCP methyltransferase, CheR-type

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 856; Mature: 855

Protein sequence:

>856_residues
MPPPTDRRPLDPLPIPIVAIGGSAGSLKPLQAFLAHLPTDSGAAYVIITHHPSGQRSLLPEILERQTELPVQMVEEGETL
GPDRIYIALPEDGWALRQGQLIRSRATETRPGGGAPYPIDAFFRSLAEEAGQGGLAVVLSGTGSDGTLGVKAIKSQSGMV
MAQDPKTAEFGDMPANAIATGQVDYVLAPADMPATLVGYLGARSERQGAPSSRRRTIPEEVLGPILRQVRHCTGHDFSGY
KTSTLERRLERRMDLLQISDPRAYSIHLQSHPEELNRLFQDFLISVTNFFRDPRAWATLSEELLPGMLRRAAHRGGEFRA
WVVGCATGEEAYTLAILIRECLADWEQAPEVRIFATDVDQAAIETARVGRYPAGIADDLTHQRLRRYFAAENDTYRIVRE
VRDMVIFADHNALQDPPFTHMDLITCRNLLIYLERDHQMRLLALFRYALRDRGLLFLGPSESPDQASEAFEVEAKEDRIY
RVAEDAARTNLLDMPGPRRFARSLPVQSTAAPAANSDGEGFSHHVERLLALRFAPPSVVVNDRGEVAYIHGRTGRFLEPA
HGPVQNQLVEMARPGLRAPLSQALEEVARGRAEEVGQRVQIQTDDDADPEWLRFEVRRIHTPAALKGFRLVTLYSEAGTE
VASLGVELEPRSESEAESEGPGTEAERRLRRELENARQDKQVAVNEMQSTNEELQSMNEELQSMNEELQSSNEELEVAKE
EVESLNQELRSVNAELQVRVDDLTETNDDLKNLLDSTHIATLFLDEALNIKRFTTPVQELIAVRPADIGRPVADLKTSLR
YDGLVADAESVLDTLIPHEVQVQSDGGDWYLLRIQPYRSTRNVIRGVVCTFQSACT

Sequences:

>Translated_856_residues
MPPPTDRRPLDPLPIPIVAIGGSAGSLKPLQAFLAHLPTDSGAAYVIITHHPSGQRSLLPEILERQTELPVQMVEEGETL
GPDRIYIALPEDGWALRQGQLIRSRATETRPGGGAPYPIDAFFRSLAEEAGQGGLAVVLSGTGSDGTLGVKAIKSQSGMV
MAQDPKTAEFGDMPANAIATGQVDYVLAPADMPATLVGYLGARSERQGAPSSRRRTIPEEVLGPILRQVRHCTGHDFSGY
KTSTLERRLERRMDLLQISDPRAYSIHLQSHPEELNRLFQDFLISVTNFFRDPRAWATLSEELLPGMLRRAAHRGGEFRA
WVVGCATGEEAYTLAILIRECLADWEQAPEVRIFATDVDQAAIETARVGRYPAGIADDLTHQRLRRYFAAENDTYRIVRE
VRDMVIFADHNALQDPPFTHMDLITCRNLLIYLERDHQMRLLALFRYALRDRGLLFLGPSESPDQASEAFEVEAKEDRIY
RVAEDAARTNLLDMPGPRRFARSLPVQSTAAPAANSDGEGFSHHVERLLALRFAPPSVVVNDRGEVAYIHGRTGRFLEPA
HGPVQNQLVEMARPGLRAPLSQALEEVARGRAEEVGQRVQIQTDDDADPEWLRFEVRRIHTPAALKGFRLVTLYSEAGTE
VASLGVELEPRSESEAESEGPGTEAERRLRRELENARQDKQVAVNEMQSTNEELQSMNEELQSMNEELQSSNEELEVAKE
EVESLNQELRSVNAELQVRVDDLTETNDDLKNLLDSTHIATLFLDEALNIKRFTTPVQELIAVRPADIGRPVADLKTSLR
YDGLVADAESVLDTLIPHEVQVQSDGGDWYLLRIQPYRSTRNVIRGVVCTFQSACT
>Mature_855_residues
PPPTDRRPLDPLPIPIVAIGGSAGSLKPLQAFLAHLPTDSGAAYVIITHHPSGQRSLLPEILERQTELPVQMVEEGETLG
PDRIYIALPEDGWALRQGQLIRSRATETRPGGGAPYPIDAFFRSLAEEAGQGGLAVVLSGTGSDGTLGVKAIKSQSGMVM
AQDPKTAEFGDMPANAIATGQVDYVLAPADMPATLVGYLGARSERQGAPSSRRRTIPEEVLGPILRQVRHCTGHDFSGYK
TSTLERRLERRMDLLQISDPRAYSIHLQSHPEELNRLFQDFLISVTNFFRDPRAWATLSEELLPGMLRRAAHRGGEFRAW
VVGCATGEEAYTLAILIRECLADWEQAPEVRIFATDVDQAAIETARVGRYPAGIADDLTHQRLRRYFAAENDTYRIVREV
RDMVIFADHNALQDPPFTHMDLITCRNLLIYLERDHQMRLLALFRYALRDRGLLFLGPSESPDQASEAFEVEAKEDRIYR
VAEDAARTNLLDMPGPRRFARSLPVQSTAAPAANSDGEGFSHHVERLLALRFAPPSVVVNDRGEVAYIHGRTGRFLEPAH
GPVQNQLVEMARPGLRAPLSQALEEVARGRAEEVGQRVQIQTDDDADPEWLRFEVRRIHTPAALKGFRLVTLYSEAGTEV
ASLGVELEPRSESEAESEGPGTEAERRLRRELENARQDKQVAVNEMQSTNEELQSMNEELQSMNEELQSSNEELEVAKEE
VESLNQELRSVNAELQVRVDDLTETNDDLKNLLDSTHIATLFLDEALNIKRFTTPVQELIAVRPADIGRPVADLKTSLRY
DGLVADAESVLDTLIPHEVQVQSDGGDWYLLRIQPYRSTRNVIRGVVCTFQSACT

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=258, Percent_Identity=30.2325581395349, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1788192, Length=177, Percent_Identity=30.5084745762712, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80 [C]

Molecular weight: Translated: 95200; Mature: 95069

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPTDRRPLDPLPIPIVAIGGSAGSLKPLQAFLAHLPTDSGAAYVIITHHPSGQRSLLP
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
EILERQTELPVQMVEEGETLGPDRIYIALPEDGWALRQGQLIRSRATETRPGGGAPYPID
HHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCCCHHCCHHHHHHCCCCCCCCCCCCCHH
AFFRSLAEEAGQGGLAVVLSGTGSDGTLGVKAIKSQSGMVMAQDPKTAEFGDMPANAIAT
HHHHHHHHHHCCCCEEEEEECCCCCCCEEHHEECCCCCEEEECCCCCCCCCCCCCCCEEC
GQVDYVLAPADMPATLVGYLGARSERQGAPSSRRRTIPEEVLGPILRQVRHCTGHDFSGY
CCEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
KTSTLERRLERRMDLLQISDPRAYSIHLQSHPEELNRLFQDFLISVTNFFRDPRAWATLS
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EELLPGMLRRAAHRGGEFRAWVVGCATGEEAYTLAILIRECLADWEQAPEVRIFATDVDQ
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHH
AAIETARVGRYPAGIADDLTHQRLRRYFAAENDTYRIVREVRDMVIFADHNALQDPPFTH
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCH
MDLITCRNLLIYLERDHQMRLLALFRYALRDRGLLFLGPSESPDQASEAFEVEAKEDRIY
HHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHEEEECCHHHHH
RVAEDAARTNLLDMPGPRRFARSLPVQSTAAPAANSDGEGFSHHVERLLALRFAPPSVVV
HHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
NDRGEVAYIHGRTGRFLEPAHGPVQNQLVEMARPGLRAPLSQALEEVARGRAEEVGQRVQ
ECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCEEE
IQTDDDADPEWLRFEVRRIHTPAALKGFRLVTLYSEAGTEVASLGVELEPRSESEAESEG
EECCCCCCHHHHHHHHHHHCCCHHHCCEEEEEEECHHCCHHHHCCCEECCCCCCCCCCCC
PGTEAERRLRRELENARQDKQVAVNEMQSTNEELQSMNEELQSMNEELQSSNEELEVAKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EVESLNQELRSVNAELQVRVDDLTETNDDLKNLLDSTHIATLFLDEALNIKRFTTPVQEL
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IAVRPADIGRPVADLKTSLRYDGLVADAESVLDTLIPHEVQVQSDGGDWYLLRIQPYRST
HHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCHHH
RNVIRGVVCTFQSACT
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PPPTDRRPLDPLPIPIVAIGGSAGSLKPLQAFLAHLPTDSGAAYVIITHHPSGQRSLLP
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
EILERQTELPVQMVEEGETLGPDRIYIALPEDGWALRQGQLIRSRATETRPGGGAPYPID
HHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCCCHHCCHHHHHHCCCCCCCCCCCCCHH
AFFRSLAEEAGQGGLAVVLSGTGSDGTLGVKAIKSQSGMVMAQDPKTAEFGDMPANAIAT
HHHHHHHHHHCCCCEEEEEECCCCCCCEEHHEECCCCCEEEECCCCCCCCCCCCCCCEEC
GQVDYVLAPADMPATLVGYLGARSERQGAPSSRRRTIPEEVLGPILRQVRHCTGHDFSGY
CCEEEEEECCCCCHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
KTSTLERRLERRMDLLQISDPRAYSIHLQSHPEELNRLFQDFLISVTNFFRDPRAWATLS
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EELLPGMLRRAAHRGGEFRAWVVGCATGEEAYTLAILIRECLADWEQAPEVRIFATDVDQ
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHH
AAIETARVGRYPAGIADDLTHQRLRRYFAAENDTYRIVREVRDMVIFADHNALQDPPFTH
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCH
MDLITCRNLLIYLERDHQMRLLALFRYALRDRGLLFLGPSESPDQASEAFEVEAKEDRIY
HHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHEEEECCHHHHH
RVAEDAARTNLLDMPGPRRFARSLPVQSTAAPAANSDGEGFSHHVERLLALRFAPPSVVV
HHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
NDRGEVAYIHGRTGRFLEPAHGPVQNQLVEMARPGLRAPLSQALEEVARGRAEEVGQRVQ
ECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCEEE
IQTDDDADPEWLRFEVRRIHTPAALKGFRLVTLYSEAGTEVASLGVELEPRSESEAESEG
EECCCCCCHHHHHHHHHHHCCCHHHCCEEEEEEECHHCCHHHHCCCEECCCCCCCCCCCC
PGTEAERRLRRELENARQDKQVAVNEMQSTNEELQSMNEELQSMNEELQSSNEELEVAKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EVESLNQELRSVNAELQVRVDDLTETNDDLKNLLDSTHIATLFLDEALNIKRFTTPVQEL
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IAVRPADIGRPVADLKTSLRYDGLVADAESVLDTLIPHEVQVQSDGGDWYLLRIQPYRST
HHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCHHH
RNVIRGVVCTFQSACT
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]

Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]