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The map label for this gene is tilS [H]

Identifier: 121998224

GI number: 121998224

Start: 1554447

End: 1555769

Strand: Reverse

Name: tilS [H]

Synonym: Hhal_1442

Alternate gene names: 121998224

Gene position: 1555769-1554447 (Counterclockwise)

Preceding gene: 121998225

Following gene: 121998222

Centisome position: 58.08

GC content: 73.39

Gene sequence:

>1323_bases
GTGCTGGACCGCCGGGTGCTGGTGGCTCGATTGCTGGGCCTGGCGCCCGCGGCCGCGGCTTACCGCGTCGCGTTCAGCGG
CGGGCGGGACTCCACCGCTTTGCTGCATGCCCTTGCCGAGGTTCGCGACCGGCTTCCGGCCCCCTTAGCCGCTGTCCACA
TCGATCACGGTCTGCATCCCGAATCGGCCCGCTGGGCCGAGGCGTGTGAGAGCGTCTGCGAGCGCCTCGGGGTGGGGCTG
GAGTGTCGCCGCGTGGTGGTTCCGCCGTCGGCCGGTCAGGGGCTGGAGGCTGCGGCCCGCGAGGCTCGCTATCGAGCCTT
CGGCGAGCTCTTGGCCGCGGGGGACTGCCTGGTTCTGGCCCACCACGCTGATGATCAGGCGGAGACCTTTCTGCTCCGGG
CGCTGCGCGGTGGGGGGTTGCACGGTCTCTGTGCCATGCCGGCCTGGCGGCCGCTGGGACGGGGCCGGCTGGTGCGTCCC
CTGCTGGAGGTGCCGCGCACGGCGCTGACCGCCTACCTGCAGTCCCGGTCCCTGGACTGGATCGAGGATCCGGCCAACCG
AGATCCCGCCCATGACCGGGTCTATCTGCGCGAGCGGGTGCTGCCGGTGCTCGCTGCACGTTGGCCGGATGCCACCGGAC
ACCTGCAGTCGGCAGTGGCCTCGTTGCAAGCGGATCAGGCCCTGCTGGGCGGGTATCTGGACCGTGATCTGGCGACGGCG
CGTGCCGCCGATGGCCGCCCCGCGGTGGCGGAGCTGCGGCGCCTCATGCCGTATCAGCGTCAGGCGGTGGTACGGCACTG
GTTGGTCTGCTCTGGCTATCGCCCGCCACCGGGGCGCCGCTTGGCCGCCGGCGTGCAGGCGCTGATCGAGTCGGGCCCGG
ATGCCCAGCCGGAGTTGCGGTGGACGGATGGGTGCATCCGGCGCTATCGGGGGCGGCTCTACCTGTTGGCTGCCGTCCCG
CCGCCTGCAGATCCCGAGCCGCGCCCGCAGGCCTGGGCCGGGCAGGGGCGGCTGATCCTCGCCGGCGGGGCGTTGTCCGC
GCAGTGGAGCCCGGCGGGGATCGATCCCGAGCGGCTGGCGCACGGGGTCACGGTGGTCTTTCGCAGCGGGGTGCTGGAGG
TGGCGGCGCGGCGCCGGCTGCAGCGGCTGTACCAGCGTCTCGGCGTGCCGCCGTGGGAGCGCGATCGGGTGCCGCTGCTG
TTCGCGGGCAGCCGCCCCGTGGCCGTGGCTGGGTACGGTATTGCGCCCGGTGAACGGGCCAGGCCGGGGTTGCGCATCGT
CTGGCAGCCGTCTCGCCCTGCGGCTCAGTCGCCGTCGAACTGA

Upstream 100 bases:

>100_bases
GCGAGGAGTGGGCGCGGGTCGGCCAGATGAGCCCGGATGAACTGGTGGCGGACCGCCACCAGCGCCTTGCCGCGATCGGC
CAGCCCCAGGGGTAACGGCC

Downstream 100 bases:

>100_bases
TCGGCCAGCGGTAGACGCAGACCGTGGCGATCGGCGGGTTCGTAGAGTCCGGTCTCGGCGTCGCCGCGGGCACTGCCGAA
ACCGACGTAGCTGTGGCGGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLDRRVLVARLLGLAPAAAAYRVAFSGGRDSTALLHALAEVRDRLPAPLAAVHIDHGLHPESARWAEACESVCERLGVGL
ECRRVVVPPSAGQGLEAAAREARYRAFGELLAAGDCLVLAHHADDQAETFLLRALRGGGLHGLCAMPAWRPLGRGRLVRP
LLEVPRTALTAYLQSRSLDWIEDPANRDPAHDRVYLRERVLPVLAARWPDATGHLQSAVASLQADQALLGGYLDRDLATA
RAADGRPAVAELRRLMPYQRQAVVRHWLVCSGYRPPPGRRLAAGVQALIESGPDAQPELRWTDGCIRRYRGRLYLLAAVP
PPADPEPRPQAWAGQGRLILAGGALSAQWSPAGIDPERLAHGVTVVFRSGVLEVAARRRLQRLYQRLGVPPWERDRVPLL
FAGSRPVAVAGYGIAPGERARPGLRIVWQPSRPAAQSPSN

Sequences:

>Translated_440_residues
MLDRRVLVARLLGLAPAAAAYRVAFSGGRDSTALLHALAEVRDRLPAPLAAVHIDHGLHPESARWAEACESVCERLGVGL
ECRRVVVPPSAGQGLEAAAREARYRAFGELLAAGDCLVLAHHADDQAETFLLRALRGGGLHGLCAMPAWRPLGRGRLVRP
LLEVPRTALTAYLQSRSLDWIEDPANRDPAHDRVYLRERVLPVLAARWPDATGHLQSAVASLQADQALLGGYLDRDLATA
RAADGRPAVAELRRLMPYQRQAVVRHWLVCSGYRPPPGRRLAAGVQALIESGPDAQPELRWTDGCIRRYRGRLYLLAAVP
PPADPEPRPQAWAGQGRLILAGGALSAQWSPAGIDPERLAHGVTVVFRSGVLEVAARRRLQRLYQRLGVPPWERDRVPLL
FAGSRPVAVAGYGIAPGERARPGLRIVWQPSRPAAQSPSN
>Mature_440_residues
MLDRRVLVARLLGLAPAAAAYRVAFSGGRDSTALLHALAEVRDRLPAPLAAVHIDHGLHPESARWAEACESVCERLGVGL
ECRRVVVPPSAGQGLEAAAREARYRAFGELLAAGDCLVLAHHADDQAETFLLRALRGGGLHGLCAMPAWRPLGRGRLVRP
LLEVPRTALTAYLQSRSLDWIEDPANRDPAHDRVYLRERVLPVLAARWPDATGHLQSAVASLQADQALLGGYLDRDLATA
RAADGRPAVAELRRLMPYQRQAVVRHWLVCSGYRPPPGRRLAAGVQALIESGPDAQPELRWTDGCIRRYRGRLYLLAAVP
PPADPEPRPQAWAGQGRLILAGGALSAQWSPAGIDPERLAHGVTVVFRSGVLEVAARRRLQRLYQRLGVPPWERDRVPLL
FAGSRPVAVAGYGIAPGERARPGLRIVWQPSRPAAQSPSN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=441, Percent_Identity=36.9614512471655, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47821; Mature: 47821

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRRVLVARLLGLAPAAAAYRVAFSGGRDSTALLHALAEVRDRLPAPLAAVHIDHGLHP
CCCHHHHHHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
ESARWAEACESVCERLGVGLECRRVVVPPSAGQGLEAAAREARYRAFGELLAAGDCLVLA
CCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
HHADDQAETFLLRALRGGGLHGLCAMPAWRPLGRGRLVRPLLEVPRTALTAYLQSRSLDW
ECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCH
IEDPANRDPAHDRVYLRERVLPVLAARWPDATGHLQSAVASLQADQALLGGYLDRDLATA
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAADGRPAVAELRRLMPYQRQAVVRHWLVCSGYRPPPGRRLAAGVQALIESGPDAQPELR
HCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
WTDGCIRRYRGRLYLLAAVPPPADPEPRPQAWAGQGRLILAGGALSAQWSPAGIDPERLA
CHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHH
HGVTVVFRSGVLEVAARRRLQRLYQRLGVPPWERDRVPLLFAGSRPVAVAGYGIAPGERA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCC
RPGLRIVWQPSRPAAQSPSN
CCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLDRRVLVARLLGLAPAAAAYRVAFSGGRDSTALLHALAEVRDRLPAPLAAVHIDHGLHP
CCCHHHHHHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
ESARWAEACESVCERLGVGLECRRVVVPPSAGQGLEAAAREARYRAFGELLAAGDCLVLA
CCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
HHADDQAETFLLRALRGGGLHGLCAMPAWRPLGRGRLVRPLLEVPRTALTAYLQSRSLDW
ECCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCH
IEDPANRDPAHDRVYLRERVLPVLAARWPDATGHLQSAVASLQADQALLGGYLDRDLATA
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAADGRPAVAELRRLMPYQRQAVVRHWLVCSGYRPPPGRRLAAGVQALIESGPDAQPELR
HCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
WTDGCIRRYRGRLYLLAAVPPPADPEPRPQAWAGQGRLILAGGALSAQWSPAGIDPERLA
CHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHH
HGVTVVFRSGVLEVAARRRLQRLYQRLGVPPWERDRVPLLFAGSRPVAVAGYGIAPGERA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCC
RPGLRIVWQPSRPAAQSPSN
CCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA