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The map label for this gene is miaA

Identifier: 121997464

GI number: 121997464

Start: 749236

End: 750168

Strand: Reverse

Name: miaA

Synonym: Hhal_0667

Alternate gene names: 121997464

Gene position: 750168-749236 (Counterclockwise)

Preceding gene: 121997465

Following gene: 121997463

Centisome position: 28.01

GC content: 71.6

Gene sequence:

>933_bases
ATGACGGCGCCGGTGCTCTGCATCATGGGGCCCACCGCGGTGGGCAAGAGCGAACTGGCCCTGTCCCTGGCCGAGCGGCT
GGGCGGTGAGATCGTCAGCGTCGACTCGGCGCAGATCTATCGCGGCCTGGATATCGGCACCGCCAAGCCGTCCCCGGCGG
TGCGTGCCCGCTGTCCCCACCACCTGATCGATATCCGCGATCCGGCCGAGCGCTACTCAGCAGCCGAGTTTGCCCGGGAC
GCCCAGGCGGCCATCGCCGCCATCCGCAGCCGGGGGCGGCTGCCGGTGCTCGTCGGTGGCACCGGGCTCTACTTCCAGGC
GCTGCAGCACGGCCTCTCGCCGATGCCGGCGGCGGACGCGCAGGTCCGTGCCGAGTTGGAGGCGGAGGAGGCGGCCTACG
GCGTTCAGGCGCTGCACCGCCGGCTGCAGGCGGTGGACCCGGAGAGTGCTGCGCGGCTGCATCCCAACGACAGTCAGCGC
ATTCAGCGGGCGCTGGAGGTGCACCGGCTGACCGGGCGGCCACTGAGCCAGGTCCAGCGTGAGCCCGGGCAGCCGGGACT
GACCGAGATGCCGTTGAAGATCATCCTCGAGCCGCCGGAGCGTGCCTGGCTGCACCGGCGTATCGAGGCGCGCTTTCGGG
CCATGCTGGCCGCCGGCCTGGTGGGCGAGGTGGTGGCGCTGCACCGCCGAGGCGATCTCAGCGAGGAACTCCCGGCGGTG
CGAGCGGTCGGATACAGACAGATCTGGCACTACCTCGAAGGCGCCTGCGATTACCGCACCATGATCCGTCGCGGCCTGCG
CGCCACGCGGCAGTACGCCAAGCGGCAGATCACCTGGCTGCGCGGGCAGACCGACGGGGTCCGGTTCACGGCGGACGACC
GGCTGGAGGCGCAGGTTCACAGTTACGTCACCGGGGCCCTCGGGGGCGTGTGA

Upstream 100 bases:

>100_bases
TCGAGCGCACCCCGCGGGCGGCGCAGTGCAACCACGGGCGGCCGACCTACACCGTGCTCGACGACGAGGCGCTGGCCCGG
CTGTTCATGCGGGGGCGGTG

Downstream 100 bases:

>100_bases
TACACTCGTAGGCGTCGAGTAGCGCCCCTCCGGCGGGGTTCGCGGGTCCATAACAACAACAATCTTGGGGGCGATGCGCC
ATGGCGAAGGGGCAGTCACT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTAPVLCIMGPTAVGKSELALSLAERLGGEIVSVDSAQIYRGLDIGTAKPSPAVRARCPHHLIDIRDPAERYSAAEFARD
AQAAIAAIRSRGRLPVLVGGTGLYFQALQHGLSPMPAADAQVRAELEAEEAAYGVQALHRRLQAVDPESAARLHPNDSQR
IQRALEVHRLTGRPLSQVQREPGQPGLTEMPLKIILEPPERAWLHRRIEARFRAMLAAGLVGEVVALHRRGDLSEELPAV
RAVGYRQIWHYLEGACDYRTMIRRGLRATRQYAKRQITWLRGQTDGVRFTADDRLEAQVHSYVTGALGGV

Sequences:

>Translated_310_residues
MTAPVLCIMGPTAVGKSELALSLAERLGGEIVSVDSAQIYRGLDIGTAKPSPAVRARCPHHLIDIRDPAERYSAAEFARD
AQAAIAAIRSRGRLPVLVGGTGLYFQALQHGLSPMPAADAQVRAELEAEEAAYGVQALHRRLQAVDPESAARLHPNDSQR
IQRALEVHRLTGRPLSQVQREPGQPGLTEMPLKIILEPPERAWLHRRIEARFRAMLAAGLVGEVVALHRRGDLSEELPAV
RAVGYRQIWHYLEGACDYRTMIRRGLRATRQYAKRQITWLRGQTDGVRFTADDRLEAQVHSYVTGALGGV
>Mature_309_residues
TAPVLCIMGPTAVGKSELALSLAERLGGEIVSVDSAQIYRGLDIGTAKPSPAVRARCPHHLIDIRDPAERYSAAEFARDA
QAAIAAIRSRGRLPVLVGGTGLYFQALQHGLSPMPAADAQVRAELEAEEAAYGVQALHRRLQAVDPESAARLHPNDSQRI
QRALEVHRLTGRPLSQVQREPGQPGLTEMPLKIILEPPERAWLHRRIEARFRAMLAAGLVGEVVALHRRGDLSEELPAVR
AVGYRQIWHYLEGACDYRTMIRRGLRATRQYAKRQITWLRGQTDGVRFTADDRLEAQVHSYVTGALGGV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=35.3503184713376, Blast_Score=168, Evalue=5e-42,
Organism=Escherichia coli, GI1790613, Length=305, Percent_Identity=51.1475409836066, Blast_Score=294, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI25144712, Length=248, Percent_Identity=30.6451612903226, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=30.3754266211604, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24649830, Length=335, Percent_Identity=29.2537313432836, Blast_Score=140, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HALHL (A1WUT7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002251.1
- ProteinModelPortal:   A1WUT7
- SMR:   A1WUT7
- STRING:   A1WUT7
- GeneID:   4710061
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0667
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A1WUT7
- BioCyc:   HHAL349124:HHAL_0667-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34112; Mature: 33980

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPVLCIMGPTAVGKSELALSLAERLGGEIVSVDSAQIYRGLDIGTAKPSPAVRARCPH
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHCCCCCCCCCCCCHHHCCCH
HLIDIRDPAERYSAAEFARDAQAAIAAIRSRGRLPVLVGGTGLYFQALQHGLSPMPAADA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCH
QVRAELEAEEAAYGVQALHRRLQAVDPESAARLHPNDSQRIQRALEVHRLTGRPLSQVQR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHC
EPGQPGLTEMPLKIILEPPERAWLHRRIEARFRAMLAAGLVGEVVALHRRGDLSEELPAV
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RAVGYRQIWHYLEGACDYRTMIRRGLRATRQYAKRQITWLRGQTDGVRFTADDRLEAQVH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHH
SYVTGALGGV
HHHHHHHCCC
>Mature Secondary Structure 
TAPVLCIMGPTAVGKSELALSLAERLGGEIVSVDSAQIYRGLDIGTAKPSPAVRARCPH
CCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHCCCCCCCCCCCCHHHCCCH
HLIDIRDPAERYSAAEFARDAQAAIAAIRSRGRLPVLVGGTGLYFQALQHGLSPMPAADA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCH
QVRAELEAEEAAYGVQALHRRLQAVDPESAARLHPNDSQRIQRALEVHRLTGRPLSQVQR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHC
EPGQPGLTEMPLKIILEPPERAWLHRRIEARFRAMLAAGLVGEVVALHRRGDLSEELPAV
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RAVGYRQIWHYLEGACDYRTMIRRGLRATRQYAKRQITWLRGQTDGVRFTADDRLEAQVH
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHH
SYVTGALGGV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA