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The map label for this gene is cysK1

Identifier: 121638221

GI number: 121638221

Start: 2585892

End: 2586824

Strand: Direct

Name: cysK1

Synonym: BCG_2356

Alternate gene names: 121638221

Gene position: 2585892-2586824 (Clockwise)

Preceding gene: 121638219

Following gene: 121638222

Centisome position: 59.11

GC content: 65.38

Gene sequence:

>933_bases
ATGAGCATCGCCGAGGACATCACCCAACTCATCGGGCGCACACCGCTGGTCCGACTGCGCCGAGTCACCGACGGCGCCGT
TGCCGACATCGTCGCCAAGCTGGAATTCTTCAACCCGGCCAACAGCGTAAAAGACCGTATCGGGGTTGCCATGCTCCAAG
CGGCCGAGCAGGCAGGTTTGATCAAGCCGGACACGATCATTCTCGAACCCACGAGCGGTAACACCGGCATCGCCCTGGCC
ATGGTTTGCGCGGCACGCGGCTACCGGTGCGTGCTGACCATGCCCGAGACGATGAGTCTGGAGCGCCGGATGTTGCTGCG
CGCATACGGTGCTGAACTCATCCTCACTCCGGGTGCGGACGGCATGTCAGGTGCCATCGCCAAGGCTGAGGAGCTGGCCA
AGACCGATCAACGCTACTTCGTGCCCCAGCAATTCGAGAACCCGGCGAACCCGGCCATCCATCGCGTCACGACCGCCGAG
GAGGTCTGGCGTGACACCGACGGCAAGGTCGACATCGTCGTCGCGGGAGTCGGCACCGGTGGCACCATCACCGGCGTCGC
GCAGGTCATCAAGGAACGCAAGCCGTCGGCCCGGTTCGTGGCCGTAGAGCCGGCCGCGTCGCCGGTCCTTTCTGGTGGCC
AGAAGGGACCGCACCCGATCCAGGGCATCGGCGCCGGGTTCGTCCCGCCGGTACTCGACCAGGACCTAGTCGACGAGATC
ATTACCGTCGGTAACGAAGACGCGCTCAACGTGGCGCGCCGGCTGGCCCGGGAAGAGGGCTTGCTGGTCGGCATCTCCTC
GGGCGCCGCCACAGTGGCCGCTCTTCAGGTGGCCCGCCGGCCAGAGAACGCCGGGAAGCTAATCGTCGTAGTGCTCCCCG
ACTTCGGCGAACGATATCTGAGCACACCGTTGTTCGCCGACGTGGCTGACTAA

Upstream 100 bases:

>100_bases
AAAGATCAACAGCCATGACCGTGCCCACGGATGCAGCCATCGACTTCGACGTCAGCTGGGAGGCCAACTGGGCCTGGACC
GACACTGTTGGGCGTAGCAG

Downstream 100 bases:

>100_bases
GCCATGCTGACGGCCATGCGGGGCGACATCCGAGCAGCCCGGGAGCGGGATCCGGCGGCCCCTACCGCGCTGGAAGTCAT
CTTCTGCTACCCGGGCGTGC

Product: putative cysteine synthase A cysK1

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGLIKPDTIILEPTSGNTGIALA
MVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGADGMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAE
EVWRDTDGKVDIVVAGVGTGGTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEI
ITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYLSTPLFADVAD

Sequences:

>Translated_310_residues
MSIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGLIKPDTIILEPTSGNTGIALA
MVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGADGMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAE
EVWRDTDGKVDIVVAGVGTGGTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEI
ITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYLSTPLFADVAD
>Mature_309_residues
SIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGLIKPDTIILEPTSGNTGIALAM
VCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGADGMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEE
VWRDTDGKVDIVVAGVGTGGTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEII
TVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYLSTPLFADVAD

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=312, Percent_Identity=41.025641025641, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI295821200, Length=312, Percent_Identity=41.025641025641, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4557415, Length=312, Percent_Identity=41.025641025641, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=56.8253968253968, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI2367138, Length=301, Percent_Identity=41.8604651162791, Blast_Score=230, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=52.4752475247525, Blast_Score=318, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=54.8701298701299, Blast_Score=312, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17562970, Length=309, Percent_Identity=51.7799352750809, Blast_Score=310, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=52.5083612040134, Blast_Score=300, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17561720, Length=305, Percent_Identity=50.1639344262295, Blast_Score=270, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI32566672, Length=217, Percent_Identity=53.4562211981567, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17534315, Length=322, Percent_Identity=40.0621118012422, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=312, Percent_Identity=37.8205128205128, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17561716, Length=105, Percent_Identity=55.2380952380952, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=37.1428571428571, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321449, Length=328, Percent_Identity=36.280487804878, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=43.3656957928803, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=43.3656957928803, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSK_MYCBO (P0A535)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856011.1
- ProteinModelPortal:   P0A535
- SMR:   P0A535
- EnsemblBacteria:   EBMYCT00000016471
- GeneID:   1094349
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2362
- GeneTree:   EBGT00050000015764
- HOGENOM:   HBG748215
- OMA:   ETIGDTP
- ProtClustDB:   CLSK799368
- BioCyc:   MBOV233413:MB2362-MONOMER
- BRENDA:   2.5.1.47
- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- TIGRFAMs:   TIGR01139
- TIGRFAMs:   TIGR01136

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =2.5.1.47

Molecular weight: Translated: 32753; Mature: 32622

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: BINDING 74-74 BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGL
CCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
IKPDTIILEPTSGNTGIALAMVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGAD
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCC
GMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEEVWRDTDGKVDIVVAGVGTG
CCCHHHHHHHHHHHHHHHEECHHHHCCCCCCHHEEECCHHHHHCCCCCCEEEEEEECCCC
GTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEI
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYL
HHCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
STPLFADVAD
CCCCCEECCC
>Mature Secondary Structure 
SIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGL
CHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
IKPDTIILEPTSGNTGIALAMVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGAD
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCCC
GMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEEVWRDTDGKVDIVVAGVGTG
CCCHHHHHHHHHHHHHHHEECHHHHCCCCCCHHEEECCHHHHHCCCCCCEEEEEEECCCC
GTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEI
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYL
HHCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
STPLFADVAD
CCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972