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The map label for this gene is murF [H]

Identifier: 121611475

GI number: 121611475

Start: 5054707

End: 5056134

Strand: Direct

Name: murF [H]

Synonym: Veis_4566

Alternate gene names: 121611475

Gene position: 5054707-5056134 (Clockwise)

Preceding gene: 121611474

Following gene: 121611476

Centisome position: 90.8

GC content: 72.83

Gene sequence:

>1428_bases
ATGATCGAGCAGCCCCGGCCCGAACCCATGTCCACACCCGTGCCCAGGCCCGCGATGATGACGCTGCAACAGGCTTTTGC
CCTGCTGCAACCGCGCATACCTGCGGCCCGCCTGGTGGGCGCCGGCGCCACGCCTGTGGCGCGCGTGCACACCGATACGC
GCACGGTGCAGGCCGGCGATCTTTTCGTTGCGCTGCGGGGCCCGCGCTTTGACGCCCACGCCTTTCTGGCCCAGGCCCGT
GCCGGTGGCGCGGCAGCGGCGCTGGCGCAGGGGGGGCTGGCGGACGCCGGTCTGCCGGGCATCGAGGTGCCCGACAGCCT
GGCCGCGCTGGCGCTTTTGGCGGCGGGCTGGCGGGCGCAGTTCGACCTGCCGCTGATCGCGGTGACGGGCAGCAACGGCA
AGACCACGGTAACGCAGATGGTGGCGGCCATATTGCGCGCCTGGCAGGGCGATGCGGCCTTGGCCACGCAGGGCAACTTC
AACAACGCGATCGGCCTGCCGCTGACGCTGCTGCGCCTGCGCGCCCGGCATACGGTCGCAGTGGTCGAGTTGGGCATGAA
CCACCCGGGCGAGATCGCCGCGCTGGCCGGCATCGCGCAGCCCGGCGTGGCGCTGGTCAACAACGCGCAGCGTGAGCATC
TGGAGTTCATGCACAGCGTGGCGGCCGTGGCGCGCGAGAACGGCTCGGTGTTCGCCAGCCTGCCGGCCCACGGGGTGGCC
GTGTTCCCGGCAGACGACGACTACCAGGCGCTGTGGCAGGAACTGGCAGCCGGCCGCGAATGCCTGCGCTTTGGCGCCGG
CGGGGCCGTCGATGGCGCCGACGCGCGCTGGGTCGATGGCGCCTGGGCGCTGACCCTGGCCACGCCGCAAGGCCCGGTCA
CGACACGCTTGCACATCGCCGGGCGGCACAACGTCGGCAATGCGCTGGCCGCCGCCGCCTGCGCGCTGGCCGCCGGCGCG
CCGCTGGCCGCGCTGGCCCAGGGCCTGAGCGGCTTCAGCCCGGTGCCGGGCCGCTCGCGCAGTTTCAGCATCCGCCACGC
GGGCCGGCAAATCAGCGTGGTCGATGACAGCTACAACGCCAACCCGGACTCGGTGCGCGCCGCCATCGCGCTGCTGGCCG
AACTGCCGGCGCCGCGGCTCCTGGTGCTCGGGGACATGGCCGAGGTGGGCGCGCAGGCCGCGCAGTTGCATGCGCAGGCG
GGCGCGCAGGCGCACGCTGCGGGCATCCACCGGCTGTTTGCGCTCGGCGCGCAGTCGGCGCATGCGGCATCGGCGTTTGC
CGGCGCGCAGCATTTCGACAGCATCGATTCGCTGTGCGCCGCCGTGCGCCAGGCCCTGCCCGATGTCGGCAGCGTGCTGG
TCAAAGGCTCCCGGAGCATGAGGATGGAGCAGGTGCTCAAAGAGATACAGGCCGGGGGTGGCGCATGA

Upstream 100 bases:

>100_bases
AGGAAGTGGCCGGCGTGCGCCGGCCATTTTCCGATTGGGCCCACGCGCAGCGCGCCATGCAGGCCCGGCAGCGCGCCGTG
CCGCCCCGGGGAGCCGGCGC

Downstream 100 bases:

>100_bases
TGCTGCTGCTGGCGCAATGGTTGCAGGGCATGTCGTCCGAACTCGGCTTCTTGCGCGTGTTCCAGTACCTGACCATGCGC
GCCGTGCTGGCCGCACTGAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MIEQPRPEPMSTPVPRPAMMTLQQAFALLQPRIPAARLVGAGATPVARVHTDTRTVQAGDLFVALRGPRFDAHAFLAQAR
AGGAAAALAQGGLADAGLPGIEVPDSLAALALLAAGWRAQFDLPLIAVTGSNGKTTVTQMVAAILRAWQGDAALATQGNF
NNAIGLPLTLLRLRARHTVAVVELGMNHPGEIAALAGIAQPGVALVNNAQREHLEFMHSVAAVARENGSVFASLPAHGVA
VFPADDDYQALWQELAAGRECLRFGAGGAVDGADARWVDGAWALTLATPQGPVTTRLHIAGRHNVGNALAAAACALAAGA
PLAALAQGLSGFSPVPGRSRSFSIRHAGRQISVVDDSYNANPDSVRAAIALLAELPAPRLLVLGDMAEVGAQAAQLHAQA
GAQAHAAGIHRLFALGAQSAHAASAFAGAQHFDSIDSLCAAVRQALPDVGSVLVKGSRSMRMEQVLKEIQAGGGA

Sequences:

>Translated_475_residues
MIEQPRPEPMSTPVPRPAMMTLQQAFALLQPRIPAARLVGAGATPVARVHTDTRTVQAGDLFVALRGPRFDAHAFLAQAR
AGGAAAALAQGGLADAGLPGIEVPDSLAALALLAAGWRAQFDLPLIAVTGSNGKTTVTQMVAAILRAWQGDAALATQGNF
NNAIGLPLTLLRLRARHTVAVVELGMNHPGEIAALAGIAQPGVALVNNAQREHLEFMHSVAAVARENGSVFASLPAHGVA
VFPADDDYQALWQELAAGRECLRFGAGGAVDGADARWVDGAWALTLATPQGPVTTRLHIAGRHNVGNALAAAACALAAGA
PLAALAQGLSGFSPVPGRSRSFSIRHAGRQISVVDDSYNANPDSVRAAIALLAELPAPRLLVLGDMAEVGAQAAQLHAQA
GAQAHAAGIHRLFALGAQSAHAASAFAGAQHFDSIDSLCAAVRQALPDVGSVLVKGSRSMRMEQVLKEIQAGGGA
>Mature_475_residues
MIEQPRPEPMSTPVPRPAMMTLQQAFALLQPRIPAARLVGAGATPVARVHTDTRTVQAGDLFVALRGPRFDAHAFLAQAR
AGGAAAALAQGGLADAGLPGIEVPDSLAALALLAAGWRAQFDLPLIAVTGSNGKTTVTQMVAAILRAWQGDAALATQGNF
NNAIGLPLTLLRLRARHTVAVVELGMNHPGEIAALAGIAQPGVALVNNAQREHLEFMHSVAAVARENGSVFASLPAHGVA
VFPADDDYQALWQELAAGRECLRFGAGGAVDGADARWVDGAWALTLATPQGPVTTRLHIAGRHNVGNALAAAACALAAGA
PLAALAQGLSGFSPVPGRSRSFSIRHAGRQISVVDDSYNANPDSVRAAIALLAELPAPRLLVLGDMAEVGAQAAQLHAQA
GAQAHAAGIHRLFALGAQSAHAASAFAGAQHFDSIDSLCAAVRQALPDVGSVLVKGSRSMRMEQVLKEIQAGGGA

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=431, Percent_Identity=44.5475638051044, Blast_Score=287, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48621; Mature: 48621

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQPRPEPMSTPVPRPAMMTLQQAFALLQPRIPAARLVGAGATPVARVHTDTRTVQAGD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCEEEECC
LFVALRGPRFDAHAFLAQARAGGAAAALAQGGLADAGLPGIEVPDSLAALALLAAGWRAQ
EEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
FDLPLIAVTGSNGKTTVTQMVAAILRAWQGDAALATQGNFNNAIGLPLTLLRLRARHTVA
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCEEE
VVELGMNHPGEIAALAGIAQPGVALVNNAQREHLEFMHSVAAVARENGSVFASLPAHGVA
EEEECCCCCCCHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEE
VFPADDDYQALWQELAAGRECLRFGAGGAVDGADARWVDGAWALTLATPQGPVTTRLHIA
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCEEEEEEECCCCCCEEEEEEE
GRHNVGNALAAAACALAAGAPLAALAQGLSGFSPVPGRSRSFSIRHAGRQISVVDDSYNA
CCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEHEECCCEEEEECCCCCC
NPDSVRAAIALLAELPAPRLLVLGDMAEVGAQAAQLHAQAGAQAHAAGIHRLFALGAQSA
CHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
HAASAFAGAQHFDSIDSLCAAVRQALPDVGSVLVKGSRSMRMEQVLKEIQAGGGA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIEQPRPEPMSTPVPRPAMMTLQQAFALLQPRIPAARLVGAGATPVARVHTDTRTVQAGD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCEEEECC
LFVALRGPRFDAHAFLAQARAGGAAAALAQGGLADAGLPGIEVPDSLAALALLAAGWRAQ
EEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
FDLPLIAVTGSNGKTTVTQMVAAILRAWQGDAALATQGNFNNAIGLPLTLLRLRARHTVA
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCEEE
VVELGMNHPGEIAALAGIAQPGVALVNNAQREHLEFMHSVAAVARENGSVFASLPAHGVA
EEEECCCCCCCHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEE
VFPADDDYQALWQELAAGRECLRFGAGGAVDGADARWVDGAWALTLATPQGPVTTRLHIA
EECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCEEEEEEECCCCCCEEEEEEE
GRHNVGNALAAAACALAAGAPLAALAQGLSGFSPVPGRSRSFSIRHAGRQISVVDDSYNA
CCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEHEECCCEEEEECCCCCC
NPDSVRAAIALLAELPAPRLLVLGDMAEVGAQAAQLHAQAGAQAHAAGIHRLFALGAQSA
CHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
HAASAFAGAQHFDSIDSLCAAVRQALPDVGSVLVKGSRSMRMEQVLKEIQAGGGA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]