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The map label for this gene is murE [H]

Identifier: 121611474

GI number: 121611474

Start: 5053157

End: 5054710

Strand: Direct

Name: murE [H]

Synonym: Veis_4565

Alternate gene names: 121611474

Gene position: 5053157-5054710 (Clockwise)

Preceding gene: 121611473

Following gene: 121611475

Centisome position: 90.77

GC content: 72.97

Gene sequence:

>1554_bases
ATGTCTGCGCCGCTGCCTGCCCCACTGCATGTGCTGCATACGCCGCATGCGCTGCAATGCGCCGGCGCGGCCTTGCCATG
GCTGCGCGCGCGCGTGAGCGGCAGTTTGCACAGCGACAGCCGCCTGATCGCCCCGGGCGACGGCTTCATCGCCTGGCCCG
GCGCCAGCACCGATGGCCGCGCCCATGTGGCCGATGCCATCGCGCGCGGCGCCACGGCCTGCCTGGTCGAGCAGGCGGGC
GTGGCGGCCTTTGCGTTCCCGGCTGCGCCTGGCGCCAGCCTGGCCAGCCTGCCGGGCCTGAAGGCCGCCGCCGGACCGAT
CGCCGCGCAGTGGTTCGATCAGCCGAGCCGCCAGCTCGATGTGCTGGCCGTCACCGGCACCAACGGCAAGACCAGCACCG
CATGGTGGCTGGCCGACGCTTTGAATGGGCGCGCAAAAAGCCCGGTCTGCGCGCTGGCGGGCACCCTGGGCCTGGGGGTT
CCCCCGGTGCTGGAAACCGCCGGCATGACCACGCCCGACCCGCTGCGCTTGCAGCGCGCACTGCGCCAATTTGTCGATCG
GGGTCTGTCGGCCTGCGCCATCGAGGCCTCGTCGATCGGCCTGGAGCAGGGGCGGCTGGCGGGCACGCAGATTCGCGCGG
CCATCTTCACCAATTGCACGCAGGACCATCTGGACTACCACGGCAGCATGGCCGCTTACTGGCGCGCCAAGCGGGCCTTG
TTCGACTGGCCCGGGTTGCAAGCGGCGGTGATCAATATCGACGACCCGGCGGGAGCGCAACTGCATGCCGAACTGGCAGG
CCGGGTGCCGGATTTGTGGAGCATTTCGACCCAAGGCCCGGCCCGCCTTGCCGCGCGCGACATCACGCTGGCGGCGCAGG
GCCTGCGCTGCACGGTGCGCGAAGGTGCCGCGCCGCAGCGGCTGACGACCCGGGTGATCGGCCACTACAACCTGCAGAAC
CTGCTGGCGGTGCTGGCCACGCTGCGCAGCCTGGGCTGGCCGCTGGCCGAGGCGCTGCAGGCCGGCGCAGGCTTGCAGCC
CGTGCCGGGCCGCATGCAGCAGTTGGCGCTGGCGGGCCAGCCGCTGGTGGTCGTGGACTATGCCCATACCCCCGATGCGC
TGGCCAAAGCCCTGCAGGCCCTGCGCCCGCTGGCCGCCCGGCGGGCTGGCAGGCTGTGGTGCGTGTTTGGCTGCGGCGGC
GACCGCGATGCCGGCAAGCGCCCGCTGATGGGCGCAGCCGCCGAGCAGCAGGCCGACCGACTGCTGCTGACCAGCGACAA
CCCGCGCAGCGAAGACCCGGACGCGATCTTGCGCCAGATCCTGCAAGGCATCAGCCGCCGCCGCGCCGTGCGCACCGAGC
CCGACCGCGCCGCCGCGATCGCGCAGGCCGTGGCCGAGGCCGCGCCCGCCGATGTGCTGCTGATTGCCGGCAAGGGCCAC
GAAGACAGCCAGGAAGTGGCCGGCGTGCGCCGGCCATTTTCCGATTGGGCCCACGCGCAGCGCGCCATGCAGGCCCGGCA
GCGCGCCGTGCCGCCCCGGGGAGCCGGCGCATGA

Upstream 100 bases:

>100_bases
TGTTCAGCAGCGTCGTGCAGCAAACGCTGCGCATGTTGGGCGTGCCGCCCGATCAGGCCGTCAAGCCGCAGATCGTGGCC
CACGTGCAGGAGCCGCTGTG

Downstream 100 bases:

>100_bases
TCGAGCAGCCCCGGCCCGAACCCATGTCCACACCCGTGCCCAGGCCCGCGATGATGACGCTGCAACAGGCTTTTGCCCTG
CTGCAACCGCGCATACCTGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSAPLPAPLHVLHTPHALQCAGAALPWLRARVSGSLHSDSRLIAPGDGFIAWPGASTDGRAHVADAIARGATACLVEQAG
VAAFAFPAAPGASLASLPGLKAAAGPIAAQWFDQPSRQLDVLAVTGTNGKTSTAWWLADALNGRAKSPVCALAGTLGLGV
PPVLETAGMTTPDPLRLQRALRQFVDRGLSACAIEASSIGLEQGRLAGTQIRAAIFTNCTQDHLDYHGSMAAYWRAKRAL
FDWPGLQAAVINIDDPAGAQLHAELAGRVPDLWSISTQGPARLAARDITLAAQGLRCTVREGAAPQRLTTRVIGHYNLQN
LLAVLATLRSLGWPLAEALQAGAGLQPVPGRMQQLALAGQPLVVVDYAHTPDALAKALQALRPLAARRAGRLWCVFGCGG
DRDAGKRPLMGAAAEQQADRLLLTSDNPRSEDPDAILRQILQGISRRRAVRTEPDRAAAIAQAVAEAAPADVLLIAGKGH
EDSQEVAGVRRPFSDWAHAQRAMQARQRAVPPRGAGA

Sequences:

>Translated_517_residues
MSAPLPAPLHVLHTPHALQCAGAALPWLRARVSGSLHSDSRLIAPGDGFIAWPGASTDGRAHVADAIARGATACLVEQAG
VAAFAFPAAPGASLASLPGLKAAAGPIAAQWFDQPSRQLDVLAVTGTNGKTSTAWWLADALNGRAKSPVCALAGTLGLGV
PPVLETAGMTTPDPLRLQRALRQFVDRGLSACAIEASSIGLEQGRLAGTQIRAAIFTNCTQDHLDYHGSMAAYWRAKRAL
FDWPGLQAAVINIDDPAGAQLHAELAGRVPDLWSISTQGPARLAARDITLAAQGLRCTVREGAAPQRLTTRVIGHYNLQN
LLAVLATLRSLGWPLAEALQAGAGLQPVPGRMQQLALAGQPLVVVDYAHTPDALAKALQALRPLAARRAGRLWCVFGCGG
DRDAGKRPLMGAAAEQQADRLLLTSDNPRSEDPDAILRQILQGISRRRAVRTEPDRAAAIAQAVAEAAPADVLLIAGKGH
EDSQEVAGVRRPFSDWAHAQRAMQARQRAVPPRGAGA
>Mature_516_residues
SAPLPAPLHVLHTPHALQCAGAALPWLRARVSGSLHSDSRLIAPGDGFIAWPGASTDGRAHVADAIARGATACLVEQAGV
AAFAFPAAPGASLASLPGLKAAAGPIAAQWFDQPSRQLDVLAVTGTNGKTSTAWWLADALNGRAKSPVCALAGTLGLGVP
PVLETAGMTTPDPLRLQRALRQFVDRGLSACAIEASSIGLEQGRLAGTQIRAAIFTNCTQDHLDYHGSMAAYWRAKRALF
DWPGLQAAVINIDDPAGAQLHAELAGRVPDLWSISTQGPARLAARDITLAAQGLRCTVREGAAPQRLTTRVIGHYNLQNL
LAVLATLRSLGWPLAEALQAGAGLQPVPGRMQQLALAGQPLVVVDYAHTPDALAKALQALRPLAARRAGRLWCVFGCGGD
RDAGKRPLMGAAAEQQADRLLLTSDNPRSEDPDAILRQILQGISRRRAVRTEPDRAAAIAQAVAEAAPADVLLIAGKGHE
DSQEVAGVRRPFSDWAHAQRAMQARQRAVPPRGAGA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=465, Percent_Identity=41.0752688172043, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54115; Mature: 53984

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPLPAPLHVLHTPHALQCAGAALPWLRARVSGSLHSDSRLIAPGDGFIAWPGASTDGR
CCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCCCCC
AHVADAIARGATACLVEQAGVAAFAFPAAPGASLASLPGLKAAAGPIAAQWFDQPSRQLD
HHHHHHHHHCHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCHHCCCHHHHHHCCCCCCEE
VLAVTGTNGKTSTAWWLADALNGRAKSPVCALAGTLGLGVPPVLETAGMTTPDPLRLQRA
EEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHH
LRQFVDRGLSACAIEASSIGLEQGRLAGTQIRAAIFTNCTQDHLDYHGSMAAYWRAKRAL
HHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHH
FDWPGLQAAVINIDDPAGAQLHAELAGRVPDLWSISTQGPARLAARDITLAAQGLRCTVR
CCCCCCEEEEEECCCCCCCEEHHHHHCCCCCCEECCCCCCHHHHHHHHHEECCCCEEEEC
EGAAPQRLTTRVIGHYNLQNLLAVLATLRSLGWPLAEALQAGAGLQPVPGRMQQLALAGQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCC
PLVVVDYAHTPDALAKALQALRPLAARRAGRLWCVFGCGGDRDAGKRPLMGAAAEQQADR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCE
LLLTSDNPRSEDPDAILRQILQGISRRRAVRTEPDRAAAIAQAVAEAAPADVLLIAGKGH
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
EDSQEVAGVRRPFSDWAHAQRAMQARQRAVPPRGAGA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAPLPAPLHVLHTPHALQCAGAALPWLRARVSGSLHSDSRLIAPGDGFIAWPGASTDGR
CCCCCCCHHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCCCCC
AHVADAIARGATACLVEQAGVAAFAFPAAPGASLASLPGLKAAAGPIAAQWFDQPSRQLD
HHHHHHHHHCHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCHHCCCHHHHHHCCCCCCEE
VLAVTGTNGKTSTAWWLADALNGRAKSPVCALAGTLGLGVPPVLETAGMTTPDPLRLQRA
EEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHH
LRQFVDRGLSACAIEASSIGLEQGRLAGTQIRAAIFTNCTQDHLDYHGSMAAYWRAKRAL
HHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHH
FDWPGLQAAVINIDDPAGAQLHAELAGRVPDLWSISTQGPARLAARDITLAAQGLRCTVR
CCCCCCEEEEEECCCCCCCEEHHHHHCCCCCCEECCCCCCHHHHHHHHHEECCCCEEEEC
EGAAPQRLTTRVIGHYNLQNLLAVLATLRSLGWPLAEALQAGAGLQPVPGRMQQLALAGQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCC
PLVVVDYAHTPDALAKALQALRPLAARRAGRLWCVFGCGGDRDAGKRPLMGAAAEQQADR
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCE
LLLTSDNPRSEDPDAILRQILQGISRRRAVRTEPDRAAAIAQAVAEAAPADVLLIAGKGH
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
EDSQEVAGVRRPFSDWAHAQRAMQARQRAVPPRGAGA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA