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The map label for this gene is mcbR [H]

Identifier: 121611403

GI number: 121611403

Start: 4969968

End: 4970663

Strand: Direct

Name: mcbR [H]

Synonym: Veis_4492

Alternate gene names: 121611403

Gene position: 4969968-4970663 (Clockwise)

Preceding gene: 121611399

Following gene: 121611404

Centisome position: 89.28

GC content: 67.53

Gene sequence:

>696_bases
GTGGCTCGTTCAACCTCGCCTTTGAAGGACTTGCCGGCGGCGCAGCCGCAGAGCCGCCAGAGCCTCAGCGCCATCGCCTA
CGACGCCCTGCGCGGCCGGCTGCGCACCGGCCTGGTGACGCCGGACGATCGCATGGTGGATATCGAGATTGCGGCGCAGT
TGGGCATGTCGCGCATGCCCGTTCGCGAGGCCCTGCTGCAACTGGTGGCAGAGGGCGTCCTGGTCAGTACCGCGCGCGGC
TATCGCATCCCCGCCCTGGATCCGAAGGACGTGATGGAAGTGTTCGAACTGCGCAAGCTGCTGGAGCCGCGTGCTGCGGC
GCTCGCCGCTCGCGACCTGTCGCGGGCGGGCATCTCGCAGTTGAGCGCAGCGCTGAGCGAGGCCAGGGCCGCCCAGGCCC
CGGAGGATTTCCCGCGCCTGTTCCGCGCCAACGTGGATTTCCGTGAGACCTGGCTGGGCGCCGTGCGCAACAAGCGGCTG
GCGGCGATGATCATGCGTTGTTCCGACCAGGTGTTGACCGTGCGGATGTCCACCTTGCGTGGGTCCGGTATCCATCCGGT
CGTGGTCGCCGGGCTGCAGGATCTGTATACGAGCTTTGCACGCCATGACTCGGTCGCCGCGCACGACAGCATGTTCAGGT
TCGTGCTGGCGGCAGAGAGCGCCTACGTAGCCCTGAGCGCAACGAGCAAGCCATGA

Upstream 100 bases:

>100_bases
CGGAAAATTGCGTTTCGTGCCCGCTTCGGCGGTCATTTTCAGAGGAAAAAAACGTGAATCGAATGCCGGATCAAGATCAA
ACCGTATACGGAAACCCATC

Downstream 100 bases:

>100_bases
ACGCCGTCCCTAGTGTCGCGTCACCGATCATCTGTCGGTCTGCGCTGGCCATCGAAGCGCATCGCGGCGTTGCATCGCTT
GCCAATACAGCTCGGTATGG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MARSTSPLKDLPAAQPQSRQSLSAIAYDALRGRLRTGLVTPDDRMVDIEIAAQLGMSRMPVREALLQLVAEGVLVSTARG
YRIPALDPKDVMEVFELRKLLEPRAAALAARDLSRAGISQLSAALSEARAAQAPEDFPRLFRANVDFRETWLGAVRNKRL
AAMIMRCSDQVLTVRMSTLRGSGIHPVVVAGLQDLYTSFARHDSVAAHDSMFRFVLAAESAYVALSATSKP

Sequences:

>Translated_231_residues
MARSTSPLKDLPAAQPQSRQSLSAIAYDALRGRLRTGLVTPDDRMVDIEIAAQLGMSRMPVREALLQLVAEGVLVSTARG
YRIPALDPKDVMEVFELRKLLEPRAAALAARDLSRAGISQLSAALSEARAAQAPEDFPRLFRANVDFRETWLGAVRNKRL
AAMIMRCSDQVLTVRMSTLRGSGIHPVVVAGLQDLYTSFARHDSVAAHDSMFRFVLAAESAYVALSATSKP
>Mature_230_residues
ARSTSPLKDLPAAQPQSRQSLSAIAYDALRGRLRTGLVTPDDRMVDIEIAAQLGMSRMPVREALLQLVAEGVLVSTARGY
RIPALDPKDVMEVFELRKLLEPRAAALAARDLSRAGISQLSAALSEARAAQAPEDFPRLFRANVDFRETWLGAVRNKRLA
AMIMRCSDQVLTVRMSTLRGSGIHPVVVAGLQDLYTSFARHDSVAAHDSMFRFVLAAESAYVALSATSKP

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=176, Percent_Identity=30.6818181818182, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25174; Mature: 25043

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSTSPLKDLPAAQPQSRQSLSAIAYDALRGRLRTGLVTPDDRMVDIEIAAQLGMSRMP
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCCCC
VREALLQLVAEGVLVSTARGYRIPALDPKDVMEVFELRKLLEPRAAALAARDLSRAGISQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LSAALSEARAAQAPEDFPRLFRANVDFRETWLGAVRNKRLAAMIMRCSDQVLTVRMSTLR
HHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GSGIHPVVVAGLQDLYTSFARHDSVAAHDSMFRFVLAAESAYVALSATSKP
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCC
>Mature Secondary Structure 
ARSTSPLKDLPAAQPQSRQSLSAIAYDALRGRLRTGLVTPDDRMVDIEIAAQLGMSRMP
CCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCCCC
VREALLQLVAEGVLVSTARGYRIPALDPKDVMEVFELRKLLEPRAAALAARDLSRAGISQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LSAALSEARAAQAPEDFPRLFRANVDFRETWLGAVRNKRLAAMIMRCSDQVLTVRMSTLR
HHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GSGIHPVVVAGLQDLYTSFARHDSVAAHDSMFRFVLAAESAYVALSATSKP
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]