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The map label for this gene is appA [H]

Identifier: 121611205

GI number: 121611205

Start: 4732732

End: 4734324

Strand: Direct

Name: appA [H]

Synonym: Veis_4290

Alternate gene names: 121611205

Gene position: 4732732-4734324 (Clockwise)

Preceding gene: 121611204

Following gene: 121611206

Centisome position: 85.02

GC content: 66.48

Gene sequence:

>1593_bases
ATGCCCCTTCTTGCCCGTTGCACATGCCGCGCCGCCTTGCTCGGCATCGGCGCCCTGCTCTCGCTGGCGCAGCCGGCCCT
GGCGGCCGATCAACCCAAGCGCGGCGGCACGCTGGTGATCGGCAGCACACAGACGCCGCGCCACCTGAACGGCGCGGTGC
AGTCGGGCGTCGCCACCGGCCTGTCCTCGACGCAGATCTTCGCCAGCCCGCTGCGCTTCGACGGGCAGTGGAACCCGCAG
CCTTACCTGGCCCAATCGTGGCGGTTGGCCGACGACGGCAAATCGCTCACGCTCGGGCTGCGCAAGGACGCGCTGTTTCA
CGACGGCAAGCCGGTCACCTCGGCCGATGTGGCTTTTTCGATCATGGCGATCAAGGCCAACCACCCGTTCAGCACCATGT
TCGGCCCGGTCGAGAAGGTCGACACGCCCGACCCGCACACCGCCATCATCCGCATGAGCGTGCCGCACCCGGCCATCGTG
CTGGCGATGTCGCCAGCCCTTTGCCCGATTCTTCCCCGGCATATCTACGGCGACGGCCAGGACCTGAAGAACCACCCGCG
CAACAGCACCGACCCGGTAGGCTCGGGCCCGTTCCGGGTCACCGAATTCCAGCCGGCGCAGCGCGTGGTGCTCGAGCGCT
TCGAGCGCTTTTTCCTGCCCGGCCGGCCCTATCTGGACAAGGTGATCGTCAGCATCACGCTCGACCGGGCCAGTCTGGTC
CTGGGGCTCGAGCGCGGCGACATACAGATGCTGCCCTTCGTCACGCAGTCCACCGACCTCAAGCGCCTGGCCAGCGACCC
GAGGCTGTCGCTCACGTCCAAGGGCTATGACGGCATGGGCCCGCTCAACTGGCTGGCCTTCAACACGGCAAAGCAGCCGC
TGTCGCACTTGCAGGTGCGCAAGGCCATCGCCACGGCCATCGACAAGAACTTCATCACCCGGGCGCTGATGGGCGGCTTT
GCCACCGTGTCCGACGGGCCGCTGGTGGCCAACAGCCCGTTTGCCGCGTCCGACCTGGTGCGCTACCCGTTCGATCTGAA
AAAAGCCGGCGCGATGCTCGACGCGGCGGGCTACAAGGCCGGCGCGAACGGCGAGCGCTTCAGGCTCGGCCTGGACTACC
TGCCCGGCAACGAAGACCAGCAAAAGAACGTGGCCGAGTACATCCGCGGCCAGCTCAAGAAGGTGGGCATCACGGTCGAG
ATGCGCGCCTCGGCCGACTTTCCGGCCTGGGCCCGGCGGATGGCCGCGCACGACTTCGACCTGTCGATGGACATGGTGTT
CAACTGGGGCGACCCGGTCATCGGCGTGCACCGCACCTACCTGTCGAGCAACATCAAACCCATCGTCTGGACCAACACCC
AGTCCTACGCCAACCCCAAGGTCGACGAATTGCTCCACACCGCCGGCAGCCTGATCGACCCGGCGCAGCGCAAAGCCCAT
TACGCCGCCTTCCAGAAGATCGTGACCGACGAGCTGCCGATCCTGTTCATCAACCAGGTGCCCTACCACACCATCACCAG
CAAGAAGCTGGCGCAGGTGCCGACCTCGATCTGGGGGCCGATGTCGCCGCTGGACGAGGTCTACTACAAGTGA

Upstream 100 bases:

>100_bases
CGTCCGGGTTCGTCCGGGCGACAGGGCTTCTTGCCCTGCCGCATTCCCGGGAGCCGATCCGGATTGCGATTGCTCGCACC
CCACTTTCAGAGGTCTTGCC

Downstream 100 bases:

>100_bases
GGGGCGGCGCCGTGGCCCTGACAGGTCTGTCCGGTATGCCGGGCATGTTGCGCCACACCGCCGCGCGGCTGCTGCAGGCC
GCCGGCCTGGTGCTCGCGGT

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MPLLARCTCRAALLGIGALLSLAQPALAADQPKRGGTLVIGSTQTPRHLNGAVQSGVATGLSSTQIFASPLRFDGQWNPQ
PYLAQSWRLADDGKSLTLGLRKDALFHDGKPVTSADVAFSIMAIKANHPFSTMFGPVEKVDTPDPHTAIIRMSVPHPAIV
LAMSPALCPILPRHIYGDGQDLKNHPRNSTDPVGSGPFRVTEFQPAQRVVLERFERFFLPGRPYLDKVIVSITLDRASLV
LGLERGDIQMLPFVTQSTDLKRLASDPRLSLTSKGYDGMGPLNWLAFNTAKQPLSHLQVRKAIATAIDKNFITRALMGGF
ATVSDGPLVANSPFAASDLVRYPFDLKKAGAMLDAAGYKAGANGERFRLGLDYLPGNEDQQKNVAEYIRGQLKKVGITVE
MRASADFPAWARRMAAHDFDLSMDMVFNWGDPVIGVHRTYLSSNIKPIVWTNTQSYANPKVDELLHTAGSLIDPAQRKAH
YAAFQKIVTDELPILFINQVPYHTITSKKLAQVPTSIWGPMSPLDEVYYK

Sequences:

>Translated_530_residues
MPLLARCTCRAALLGIGALLSLAQPALAADQPKRGGTLVIGSTQTPRHLNGAVQSGVATGLSSTQIFASPLRFDGQWNPQ
PYLAQSWRLADDGKSLTLGLRKDALFHDGKPVTSADVAFSIMAIKANHPFSTMFGPVEKVDTPDPHTAIIRMSVPHPAIV
LAMSPALCPILPRHIYGDGQDLKNHPRNSTDPVGSGPFRVTEFQPAQRVVLERFERFFLPGRPYLDKVIVSITLDRASLV
LGLERGDIQMLPFVTQSTDLKRLASDPRLSLTSKGYDGMGPLNWLAFNTAKQPLSHLQVRKAIATAIDKNFITRALMGGF
ATVSDGPLVANSPFAASDLVRYPFDLKKAGAMLDAAGYKAGANGERFRLGLDYLPGNEDQQKNVAEYIRGQLKKVGITVE
MRASADFPAWARRMAAHDFDLSMDMVFNWGDPVIGVHRTYLSSNIKPIVWTNTQSYANPKVDELLHTAGSLIDPAQRKAH
YAAFQKIVTDELPILFINQVPYHTITSKKLAQVPTSIWGPMSPLDEVYYK
>Mature_529_residues
PLLARCTCRAALLGIGALLSLAQPALAADQPKRGGTLVIGSTQTPRHLNGAVQSGVATGLSSTQIFASPLRFDGQWNPQP
YLAQSWRLADDGKSLTLGLRKDALFHDGKPVTSADVAFSIMAIKANHPFSTMFGPVEKVDTPDPHTAIIRMSVPHPAIVL
AMSPALCPILPRHIYGDGQDLKNHPRNSTDPVGSGPFRVTEFQPAQRVVLERFERFFLPGRPYLDKVIVSITLDRASLVL
GLERGDIQMLPFVTQSTDLKRLASDPRLSLTSKGYDGMGPLNWLAFNTAKQPLSHLQVRKAIATAIDKNFITRALMGGFA
TVSDGPLVANSPFAASDLVRYPFDLKKAGAMLDAAGYKAGANGERFRLGLDYLPGNEDQQKNVAEYIRGQLKKVGITVEM
RASADFPAWARRMAAHDFDLSMDMVFNWGDPVIGVHRTYLSSNIKPIVWTNTQSYANPKVDELLHTAGSLIDPAQRKAHY
AAFQKIVTDELPILFINQVPYHTITSKKLAQVPTSIWGPMSPLDEVYYK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=521, Percent_Identity=25.1439539347409, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787762, Length=548, Percent_Identity=27.007299270073, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787052, Length=513, Percent_Identity=24.5614035087719, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789887, Length=488, Percent_Identity=23.5655737704918, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1787551, Length=453, Percent_Identity=24.9448123620309, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082063, Length=275, Percent_Identity=25.4545454545455, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787495, Length=554, Percent_Identity=22.7436823104693, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58004; Mature: 57873

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLARCTCRAALLGIGALLSLAQPALAADQPKRGGTLVIGSTQTPRHLNGAVQSGVATG
CCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
LSSTQIFASPLRFDGQWNPQPYLAQSWRLADDGKSLTLGLRKDALFHDGKPVTSADVAFS
CCCCHHEECCCCCCCCCCCCCHHHCCCEECCCCCEEEEEECCCHHHCCCCCCCCCCEEEE
IMAIKANHPFSTMFGPVEKVDTPDPHTAIIRMSVPHPAIVLAMSPALCPILPRHIYGDGQ
EEEEECCCCHHHHCCCHHHCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHCCCCC
DLKNHPRNSTDPVGSGPFRVTEFQPAQRVVLERFERFFLPGRPYLDKVIVSITLDRASLV
HHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCEEEE
LGLERGDIQMLPFVTQSTDLKRLASDPRLSLTSKGYDGMGPLNWLAFNTAKQPLSHLQVR
EEECCCCEEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCHHEEECCHHHHHHHHHHH
KAIATAIDKNFITRALMGGFATVSDGPLVANSPFAASDLVRYPFDLKKAGAMLDAAGYKA
HHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCCHHHHHHCCCCHHHHCCEEECCCCCC
GANGERFRLGLDYLPGNEDQQKNVAEYIRGQLKKVGITVEMRASADFPAWARRMAAHDFD
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCC
LSMDMVFNWGDPVIGVHRTYLSSNIKPIVWTNTQSYANPKVDELLHTAGSLIDPAQRKAH
CEEEEEEECCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHH
YAAFQKIVTDELPILFINQVPYHTITSKKLAQVPTSIWGPMSPLDEVYYK
HHHHHHHHHCCCCEEEECCCCCCHHCHHHHHHCCHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
PLLARCTCRAALLGIGALLSLAQPALAADQPKRGGTLVIGSTQTPRHLNGAVQSGVATG
CCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCC
LSSTQIFASPLRFDGQWNPQPYLAQSWRLADDGKSLTLGLRKDALFHDGKPVTSADVAFS
CCCCHHEECCCCCCCCCCCCCHHHCCCEECCCCCEEEEEECCCHHHCCCCCCCCCCEEEE
IMAIKANHPFSTMFGPVEKVDTPDPHTAIIRMSVPHPAIVLAMSPALCPILPRHIYGDGQ
EEEEECCCCHHHHCCCHHHCCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHCCCCC
DLKNHPRNSTDPVGSGPFRVTEFQPAQRVVLERFERFFLPGRPYLDKVIVSITLDRASLV
HHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCEEEE
LGLERGDIQMLPFVTQSTDLKRLASDPRLSLTSKGYDGMGPLNWLAFNTAKQPLSHLQVR
EEECCCCEEEEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCHHEEECCHHHHHHHHHHH
KAIATAIDKNFITRALMGGFATVSDGPLVANSPFAASDLVRYPFDLKKAGAMLDAAGYKA
HHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCCHHHHHHCCCCHHHHCCEEECCCCCC
GANGERFRLGLDYLPGNEDQQKNVAEYIRGQLKKVGITVEMRASADFPAWARRMAAHDFD
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCC
LSMDMVFNWGDPVIGVHRTYLSSNIKPIVWTNTQSYANPKVDELLHTAGSLIDPAQRKAH
CEEEEEEECCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHH
YAAFQKIVTDELPILFINQVPYHTITSKKLAQVPTSIWGPMSPLDEVYYK
HHHHHHHHHCCCCEEEECCCCCCHHCHHHHHHCCHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]