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The map label for this gene is puuB [H]

Identifier: 121611204

GI number: 121611204

Start: 4730810

End: 4732309

Strand: Direct

Name: puuB [H]

Synonym: Veis_4289

Alternate gene names: 121611204

Gene position: 4730810-4732309 (Clockwise)

Preceding gene: 121611199

Following gene: 121611205

Centisome position: 84.98

GC content: 70.13

Gene sequence:

>1500_bases
ATGCGCACGAGCACCGCAGCAGACATCCGGGGCATGGCCAGCGCCGCGCCGATGGCGCCACCGGCAGCCCTTGCACACCC
CTCGCCCCCCTCGCCCCCCTCGCCTCCATTGCGCCCCTACGACCCGCAGTACGACCCGCTGCACGCGCGCGACCCCGGCA
CCGGCTGCGCCTATGCGCCCACCTGGTGGGTGGCCAGCGCCGGCCAGCCGCCCGGGGACGACGGCCCGCTGGTGCGCGAC
ATCGATGTCGATGTGGCCGTCATCGGGTCGGGCAGCACCGGCCTGTCGACCGCGCTGTACCTGGCGCAGGAGCATGGCAT
CCGCGCGACCGTGCTCGAAGCCAACCAGACCGCCTGGGGCTGCTCGAGCCGCAGCGGCGGCCAGGGCCAGAACGCCGGCG
GCCGGCTCAAGCGCTCGCAGTGGATCGCGCGCTGGGGCCTGGACACGGCCAGGAAGCTCGACGCGGAAATTCGCAGCGGC
TTCGAGAACTTTCGCCACCTGAGCCGCCAGATCGACTGCGAGGCCCATGACGGCGGCCACCTCTACCTGGCGCACCGGCG
CGAGAAACTCGCCGGCCTCGACGCCGAGGCCCGGCTGATGCGCGAGACCTTCGGCTACGCCACGCGCATGCTGAGCGCCG
AGCAAGTGCGGCAAGACTACTGCGACGAGCGCGAGGCCGTCGGCGCCATGTTCGAGCCTGAAGGCGTGGGCATCCACCCG
CTCAAGTTCAGTTTCGGCCTGATGCGCAAAGCGCGCGCGCTCGGCGTGACGGTCCATACCGCAAGCCCGGTGCAGGGCTG
GCACAGCGCCGCCGGCTGGCAGCATCTGCAAACACCCGCCGGCCTGGTGCGGGCGCGGCGCGTGGCGGTATGCACCGGCG
GCTACACGGGCCAGACGCTCAACCCGCTGCTCAAGGACCGGGTCATGCCGATCCTGTCGAACTCGGTGGTCACGCGGGTG
CTCAGCCCGGCCGAACTGGCCGCCACCAACTTCAGGACCGAAACCTTCCTGACCGATACGCGCACGCTGCGCTTTTACTA
CCGCTTGCTCAGGGACAAGCGGCTGCAGATCGGCAGCCGCAGCGCGGTCAGCGGCGCCGATGCCGAGCACCCGGCGCACC
TGCGGCTGCTGACCGACGCCATTGCGCGCAAGTTCCCGCCGCTGGCCGGCATCCGCATCGACTACTCGTGGTGGGGCTGG
GTCGACATGAGCCACGACATGATGCCGCGCATCACCCAGCCCGATGCGCAGCAAAAAATCTGGTACGCGCTGGGCTATGG
CGGCAACGGCGTGTCGTTCTCGACCTGGGCCGGCAAGCGGCTGGCCGAGCGCGTGGCGGGCAAGGACGGCGGCCGGGAAC
TGTTCGATCTGCCGATCTATCGCTCGCCTTTGCCTTTCCCGAACCTGATGGGCCTGGCTCAGACGCAGGCCCTGGCGCCT
TTTCGCCGCATCGGCCAGCGCATGCTCTACCAGTGGTACTGGCTGCGTGACGAGAAGTAG

Upstream 100 bases:

>100_bases
CCGACGGCATCGATGTGTTCACCTTCAGGGACGGAAAAATCCACCTGAAGAACGTGTTCCGCAAGGTGCGCCCGAACCTG
CCCGCGCCCCGGTGACAGCG

Downstream 100 bases:

>100_bases
CCGCCTCCTCTAGTGTCGCGTCACCGATCATCTGTCGGTCTGCGCTGGCCATCGAAGCGCATCGCGGCGTTGCATCGCTT
GCCAATACAGCTCGGTATGG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MRTSTAADIRGMASAAPMAPPAALAHPSPPSPPSPPLRPYDPQYDPLHARDPGTGCAYAPTWWVASAGQPPGDDGPLVRD
IDVDVAVIGSGSTGLSTALYLAQEHGIRATVLEANQTAWGCSSRSGGQGQNAGGRLKRSQWIARWGLDTARKLDAEIRSG
FENFRHLSRQIDCEAHDGGHLYLAHRREKLAGLDAEARLMRETFGYATRMLSAEQVRQDYCDEREAVGAMFEPEGVGIHP
LKFSFGLMRKARALGVTVHTASPVQGWHSAAGWQHLQTPAGLVRARRVAVCTGGYTGQTLNPLLKDRVMPILSNSVVTRV
LSPAELAATNFRTETFLTDTRTLRFYYRLLRDKRLQIGSRSAVSGADAEHPAHLRLLTDAIARKFPPLAGIRIDYSWWGW
VDMSHDMMPRITQPDAQQKIWYALGYGGNGVSFSTWAGKRLAERVAGKDGGRELFDLPIYRSPLPFPNLMGLAQTQALAP
FRRIGQRMLYQWYWLRDEK

Sequences:

>Translated_499_residues
MRTSTAADIRGMASAAPMAPPAALAHPSPPSPPSPPLRPYDPQYDPLHARDPGTGCAYAPTWWVASAGQPPGDDGPLVRD
IDVDVAVIGSGSTGLSTALYLAQEHGIRATVLEANQTAWGCSSRSGGQGQNAGGRLKRSQWIARWGLDTARKLDAEIRSG
FENFRHLSRQIDCEAHDGGHLYLAHRREKLAGLDAEARLMRETFGYATRMLSAEQVRQDYCDEREAVGAMFEPEGVGIHP
LKFSFGLMRKARALGVTVHTASPVQGWHSAAGWQHLQTPAGLVRARRVAVCTGGYTGQTLNPLLKDRVMPILSNSVVTRV
LSPAELAATNFRTETFLTDTRTLRFYYRLLRDKRLQIGSRSAVSGADAEHPAHLRLLTDAIARKFPPLAGIRIDYSWWGW
VDMSHDMMPRITQPDAQQKIWYALGYGGNGVSFSTWAGKRLAERVAGKDGGRELFDLPIYRSPLPFPNLMGLAQTQALAP
FRRIGQRMLYQWYWLRDEK
>Mature_499_residues
MRTSTAADIRGMASAAPMAPPAALAHPSPPSPPSPPLRPYDPQYDPLHARDPGTGCAYAPTWWVASAGQPPGDDGPLVRD
IDVDVAVIGSGSTGLSTALYLAQEHGIRATVLEANQTAWGCSSRSGGQGQNAGGRLKRSQWIARWGLDTARKLDAEIRSG
FENFRHLSRQIDCEAHDGGHLYLAHRREKLAGLDAEARLMRETFGYATRMLSAEQVRQDYCDEREAVGAMFEPEGVGIHP
LKFSFGLMRKARALGVTVHTASPVQGWHSAAGWQHLQTPAGLVRARRVAVCTGGYTGQTLNPLLKDRVMPILSNSVVTRV
LSPAELAATNFRTETFLTDTRTLRFYYRLLRDKRLQIGSRSAVSGADAEHPAHLRLLTDAIARKFPPLAGIRIDYSWWGW
VDMSHDMMPRITQPDAQQKIWYALGYGGNGVSFSTWAGKRLAERVAGKDGGRELFDLPIYRSPLPFPNLMGLAQTQALAP
FRRIGQRMLYQWYWLRDEK

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=449, Percent_Identity=32.0712694877506, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55077; Mature: 55077

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSTAADIRGMASAAPMAPPAALAHPSPPSPPSPPLRPYDPQYDPLHARDPGTGCAYAP
CCCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TWWVASAGQPPGDDGPLVRDIDVDVAVIGSGSTGLSTALYLAQEHGIRATVLEANQTAWG
CCEEECCCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
CSSRSGGQGQNAGGRLKRSQWIARWGLDTARKLDAEIRSGFENFRHLSRQIDCEAHDGGH
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCE
LYLAHRREKLAGLDAEARLMRETFGYATRMLSAEQVRQDYCDEREAVGAMFEPEGVGIHP
EEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEECCCCCCEEE
LKFSFGLMRKARALGVTVHTASPVQGWHSAAGWQHLQTPAGLVRARRVAVCTGGYTGQTL
HHHHHHHHHHHHHHEEEEECCCCCCHHHHHCCCHHHCCHHHHHHHCEEEEEECCCCCCHH
NPLLKDRVMPILSNSVVTRVLSPAELAATNFRTETFLTDTRTLRFYYRLLRDKRLQIGSR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCC
SAVSGADAEHPAHLRLLTDAIARKFPPLAGIRIDYSWWGWVDMSHDMMPRITQPDAQQKI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCE
WYALGYGGNGVSFSTWAGKRLAERVAGKDGGRELFDLPIYRSPLPFPNLMGLAQTQALAP
EEEEEECCCCCEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
FRRIGQRMLYQWYWLRDEK
HHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MRTSTAADIRGMASAAPMAPPAALAHPSPPSPPSPPLRPYDPQYDPLHARDPGTGCAYAP
CCCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TWWVASAGQPPGDDGPLVRDIDVDVAVIGSGSTGLSTALYLAQEHGIRATVLEANQTAWG
CCEEECCCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
CSSRSGGQGQNAGGRLKRSQWIARWGLDTARKLDAEIRSGFENFRHLSRQIDCEAHDGGH
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCE
LYLAHRREKLAGLDAEARLMRETFGYATRMLSAEQVRQDYCDEREAVGAMFEPEGVGIHP
EEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEECCCCCCEEE
LKFSFGLMRKARALGVTVHTASPVQGWHSAAGWQHLQTPAGLVRARRVAVCTGGYTGQTL
HHHHHHHHHHHHHHEEEEECCCCCCHHHHHCCCHHHCCHHHHHHHCEEEEEECCCCCCHH
NPLLKDRVMPILSNSVVTRVLSPAELAATNFRTETFLTDTRTLRFYYRLLRDKRLQIGSR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCC
SAVSGADAEHPAHLRLLTDAIARKFPPLAGIRIDYSWWGWVDMSHDMMPRITQPDAQQKI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCE
WYALGYGGNGVSFSTWAGKRLAERVAGKDGGRELFDLPIYRSPLPFPNLMGLAQTQALAP
EEEEEECCCCCEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
FRRIGQRMLYQWYWLRDEK
HHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]