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The map label for this gene is shiA [H]

Identifier: 121611199

GI number: 121611199

Start: 4724299

End: 4725681

Strand: Direct

Name: shiA [H]

Synonym: Veis_4283

Alternate gene names: 121611199

Gene position: 4724299-4725681 (Clockwise)

Preceding gene: 121611198

Following gene: 121611204

Centisome position: 84.87

GC content: 66.02

Gene sequence:

>1383_bases
ATGAACACGAGCGCCAGCGACCGGAAAGCGATTCGCAGGGTGGTGATTTCGGCCCTGGTGGGCGCCACCATCGAGTGGTA
TGACTTTTTTCTGTACGGCGTGGTGGCCGGCATCGTGTTCAACAAGCTCTACTTTCCGGGCAGCGATCCGGTGGTCTCGA
CCCTGCTGGCCTACACCACCTTCGCCGTGGGCTTCGTCACGCGGCCGCTGGGCGGCGTGATCTTTGGCCACTTCGGCGAC
AGGATCGGCCGGAAGAGCATGTTGATCATCACGCTGATGATCATGGGCGTGGCCACCTTCCTGATCGGGCTGGTGCCCAC
CTACGCGCAGATCGGCATTGCCGCGCCGCTGCTGTTGCTGCTGCTGCGCATCGCGCAGGGCATAGGCCTGGGCGGCGAAT
GGGGCGGCGCGGTGCTGATGGCCTACGAGTACGCGCCCAAGGAGCAGCGCGGCTGGTACGCCTCGCTGCCGCAGATCGGG
CTGGCCATCGGCCTGTGCCTGGCCTCGGGCGTGGTCGCGCTGCTGTCGGGCCTGCTCAGCGACGCGCAGTTCCTCTCGTG
GGGCTGGCGCATCGCCTTCCTGATCTCGGGCGCGATGGTCGGGGTGGGCCTGTACATCCGCCTGCATGTGCAGGAAACGC
CCGAGTTCGCGGCCGTGAAGGCGCGCAACGCCGAGTTGCGCGTTCCGTTCATCGACATGCTCCGCCGCTACCCCGGCAAC
GTGCTCAAGGGCATGGGCGCGCGCTACATCGACGGCGTGTTCTTCAACATCTTCGGCGTGTTCTCGATCAACTACCTGAC
CGGCACGCTCAGCGTTAGCCGCACCGACGCCTTGCTGGGCGTGATGGCCGCAGCGGTGGTGATGATCTTCTTCATCCCCT
TTTTCGGCCGGCTCTCGGACCAGCTCGGGCGCAGCAGGGTTTTCATGTGGGGTTCGCTGATCACCGCCTCGTCGGCGTTC
CCGGGCTTTTGGCTGATGACCCACAGCGGCGGCAACATGGCCTTGGTCTGGCTCGCGATCATCGTGCCCTTCGGCATCCT
GTACGCCTCGGTGTACGGACCGGAGGCGGCCCTGTTCTGCGAGCTGTTCGATGCCAAGGTGCGCTACACGGGCATCTCCT
TCGTCTACCAGTTCTCGGGCATTTTTGCCTCCGGCATCACGCCGATCATCGCCACCGCGCTGCTCAAGTCGGGCGGCGGC
CAGCCCTGGCAAATCTGCCTGTACCTGCTGTTCGCGGGCGCGGTGTCGGCGCTGTGCGCATGGCTCATCGGGCGCAGTCC
GGCACCCGGGCCGGCGCCGGCTACATGCGCCCTTTCCAGGGCACCAGACGGCGCTCGAGCCAGCGCATCAGCAGGTCGAA
CAGGTACGCCACCACCCCGATGA

Upstream 100 bases:

>100_bases
GGCCGCCGGTTTTTCCTGCCCCGCTTCACCCCGACCGCGAGCGCCCGCAGAGGCGGCGGCCACGACAACCGATCGACAAC
CGATCATCGGAGACAAACAC

Downstream 100 bases:

>100_bases
CGATGATGCCCATGATGACGATGTCGGTGCGCAGGAAGTTCGACGCATTCAGCACCATCTGCCCCAGGCCAATGTCGGCG
GCCACCATCTCGGCAGCCAC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNTSASDRKAIRRVVISALVGATIEWYDFFLYGVVAGIVFNKLYFPGSDPVVSTLLAYTTFAVGFVTRPLGGVIFGHFGD
RIGRKSMLIITLMIMGVATFLIGLVPTYAQIGIAAPLLLLLLRIAQGIGLGGEWGGAVLMAYEYAPKEQRGWYASLPQIG
LAIGLCLASGVVALLSGLLSDAQFLSWGWRIAFLISGAMVGVGLYIRLHVQETPEFAAVKARNAELRVPFIDMLRRYPGN
VLKGMGARYIDGVFFNIFGVFSINYLTGTLSVSRTDALLGVMAAAVVMIFFIPFFGRLSDQLGRSRVFMWGSLITASSAF
PGFWLMTHSGGNMALVWLAIIVPFGILYASVYGPEAALFCELFDAKVRYTGISFVYQFSGIFASGITPIIATALLKSGGG
QPWQICLYLLFAGAVSALCAWLIGRSPAPGPAPATCALSRAPDGARASASAGRTGTPPPR

Sequences:

>Translated_460_residues
MNTSASDRKAIRRVVISALVGATIEWYDFFLYGVVAGIVFNKLYFPGSDPVVSTLLAYTTFAVGFVTRPLGGVIFGHFGD
RIGRKSMLIITLMIMGVATFLIGLVPTYAQIGIAAPLLLLLLRIAQGIGLGGEWGGAVLMAYEYAPKEQRGWYASLPQIG
LAIGLCLASGVVALLSGLLSDAQFLSWGWRIAFLISGAMVGVGLYIRLHVQETPEFAAVKARNAELRVPFIDMLRRYPGN
VLKGMGARYIDGVFFNIFGVFSINYLTGTLSVSRTDALLGVMAAAVVMIFFIPFFGRLSDQLGRSRVFMWGSLITASSAF
PGFWLMTHSGGNMALVWLAIIVPFGILYASVYGPEAALFCELFDAKVRYTGISFVYQFSGIFASGITPIIATALLKSGGG
QPWQICLYLLFAGAVSALCAWLIGRSPAPGPAPATCALSRAPDGARASASAGRTGTPPPR
>Mature_460_residues
MNTSASDRKAIRRVVISALVGATIEWYDFFLYGVVAGIVFNKLYFPGSDPVVSTLLAYTTFAVGFVTRPLGGVIFGHFGD
RIGRKSMLIITLMIMGVATFLIGLVPTYAQIGIAAPLLLLLLRIAQGIGLGGEWGGAVLMAYEYAPKEQRGWYASLPQIG
LAIGLCLASGVVALLSGLLSDAQFLSWGWRIAFLISGAMVGVGLYIRLHVQETPEFAAVKARNAELRVPFIDMLRRYPGN
VLKGMGARYIDGVFFNIFGVFSINYLTGTLSVSRTDALLGVMAAAVVMIFFIPFFGRLSDQLGRSRVFMWGSLITASSAF
PGFWLMTHSGGNMALVWLAIIVPFGILYASVYGPEAALFCELFDAKVRYTGISFVYQFSGIFASGITPIIATALLKSGGG
QPWQICLYLLFAGAVSALCAWLIGRSPAPGPAPATCALSRAPDGARASASAGRTGTPPPR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=40.9090909090909, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1789941, Length=396, Percent_Identity=39.3939393939394, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1790550, Length=453, Percent_Identity=31.5673289183223, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=30.3317535545024, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI87082231, Length=291, Percent_Identity=23.3676975945017, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082404, Length=393, Percent_Identity=24.1730279898219, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49143; Mature: 49143

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSASDRKAIRRVVISALVGATIEWYDFFLYGVVAGIVFNKLYFPGSDPVVSTLLAYTT
CCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFVTRPLGGVIFGHFGDRIGRKSMLIITLMIMGVATFLIGLVPTYAQIGIAAPLLLL
HHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRIAQGIGLGGEWGGAVLMAYEYAPKEQRGWYASLPQIGLAIGLCLASGVVALLSGLLS
HHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAQFLSWGWRIAFLISGAMVGVGLYIRLHVQETPEFAAVKARNAELRVPFIDMLRRYPGN
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHEEECCCEEECCHHHHHHHCCCH
VLKGMGARYIDGVFFNIFGVFSINYLTGTLSVSRTDALLGVMAAAVVMIFFIPFFGRLSD
HHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGRSRVFMWGSLITASSAFPGFWLMTHSGGNMALVWLAIIVPFGILYASVYGPEAALFC
HHCCCCEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ELFDAKVRYTGISFVYQFSGIFASGITPIIATALLKSGGGQPWQICLYLLFAGAVSALCA
HHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
WLIGRSPAPGPAPATCALSRAPDGARASASAGRTGTPPPR
HHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTSASDRKAIRRVVISALVGATIEWYDFFLYGVVAGIVFNKLYFPGSDPVVSTLLAYTT
CCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFVTRPLGGVIFGHFGDRIGRKSMLIITLMIMGVATFLIGLVPTYAQIGIAAPLLLL
HHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRIAQGIGLGGEWGGAVLMAYEYAPKEQRGWYASLPQIGLAIGLCLASGVVALLSGLLS
HHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAQFLSWGWRIAFLISGAMVGVGLYIRLHVQETPEFAAVKARNAELRVPFIDMLRRYPGN
HHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCHHEEECCCEEECCHHHHHHHCCCH
VLKGMGARYIDGVFFNIFGVFSINYLTGTLSVSRTDALLGVMAAAVVMIFFIPFFGRLSD
HHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLGRSRVFMWGSLITASSAFPGFWLMTHSGGNMALVWLAIIVPFGILYASVYGPEAALFC
HHCCCCEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ELFDAKVRYTGISFVYQFSGIFASGITPIIATALLKSGGGQPWQICLYLLFAGAVSALCA
HHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
WLIGRSPAPGPAPATCALSRAPDGARASASAGRTGTPPPR
HHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]