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The map label for this gene is pilD [H]

Identifier: 121610839

GI number: 121610839

Start: 4309488

End: 4310372

Strand: Direct

Name: pilD [H]

Synonym: Veis_3921

Alternate gene names: 121610839

Gene position: 4309488-4310372 (Clockwise)

Preceding gene: 121610838

Following gene: 121610840

Centisome position: 77.41

GC content: 64.18

Gene sequence:

>885_bases
ATGCTTGCGGGCCTGTGGCTGGACGCGGCATTGGGCGGCGTGATCGGTCTGCTTTTCGGCAGCTTTTTGAATGTGGTCAT
TTACCGCCTGCCGAAGATGATGGAGCGGCAGTGGGCGGCTGAGTTCGCCCACCGGGATGCCCCGCATCCGGCAGCGCAGG
CGCCTGCCGTGCCCGCCGAGGCTGCGCCCGAGCCTTTCAATCTGCTGATTCCCCGTTCGCGCTGCCCGGCCTGTGGGCAT
ATGCTGCCGTGGTACGAAAATGTCCCGGTGCTGAGCTACCTTGCGCTGCGGGGGCGCTGCTCGGCTTGCCGGGCGCGCAT
CAGTGCGCGCTACCCGCTGGTGGAGATCGTGACGGGTGGGTTGTTCTTTTTTTGCATCGAGCGCTGGGGCCTGACAGCGG
CTGGCATTGCGTGGTGCGGCTTTTCTGCCGCCTCGATGGCCTTGGCATGCATCGACTGGGATACCACCCTGTTGCCGGAT
GACATCACTTTGCCCTTGCTGTGGGCCGGATTGCTGTCGGCTGCGCTGCAGTGGATCGATCTGCCGCTGTACGACTCGGT
GCTGGGGGCGGTTGCAGGGTATTTGTCGCTTTGGCTGGTCTACTGGGGTTTCAAGTTGGCCACCGGCAAGGAGGGCATGG
GCTATGGCGACTTCAAGCTGTTCGCCGCTTTGGGGGCCTGGTTTGGCTGGCAAGCGTTGGTGCCCATCATTTTGCTGTCT
TCGGTGATCGGCGCTGGCGTCGGTATCGCGATGAAGATTTCCAGCCGCTTGCGTGAGGGGGGTTATCTCCCCTTCGGGCC
TTTTCTGGTCGGCGCCGGTCTGACGGCGCTGGTGTTCGGCCCCGGCACCATGCTGGGCCTGGCGCTCGGGCTGCTGGGCC
TTTGA

Upstream 100 bases:

>100_bases
GCCTGATGGAGCCGATCATCATCGTTTTTCTTGGCTGCCTGATCGGCGGCATCGTCATCTCGATGTATCTGCCGATCTTC
AAATTGGGACAGGTCGTGTA

Downstream 100 bases:

>100_bases
TCCATGCGCCGCAAGCCATCGCGTCCATTGCGTGTGGGCCTGACCGGCGGCATTGGCAGCGGCAAGAGCACGGTGGGGCA
GATGCTGGCGGCCCTGGGGG

Product: prepilin peptidase

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLAGLWLDAALGGVIGLLFGSFLNVVIYRLPKMMERQWAAEFAHRDAPHPAAQAPAVPAEAAPEPFNLLIPRSRCPACGH
MLPWYENVPVLSYLALRGRCSACRARISARYPLVEIVTGGLFFFCIERWGLTAAGIAWCGFSAASMALACIDWDTTLLPD
DITLPLLWAGLLSAALQWIDLPLYDSVLGAVAGYLSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILLS
SVIGAGVGIAMKISSRLREGGYLPFGPFLVGAGLTALVFGPGTMLGLALGLLGL

Sequences:

>Translated_294_residues
MLAGLWLDAALGGVIGLLFGSFLNVVIYRLPKMMERQWAAEFAHRDAPHPAAQAPAVPAEAAPEPFNLLIPRSRCPACGH
MLPWYENVPVLSYLALRGRCSACRARISARYPLVEIVTGGLFFFCIERWGLTAAGIAWCGFSAASMALACIDWDTTLLPD
DITLPLLWAGLLSAALQWIDLPLYDSVLGAVAGYLSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILLS
SVIGAGVGIAMKISSRLREGGYLPFGPFLVGAGLTALVFGPGTMLGLALGLLGL
>Mature_294_residues
MLAGLWLDAALGGVIGLLFGSFLNVVIYRLPKMMERQWAAEFAHRDAPHPAAQAPAVPAEAAPEPFNLLIPRSRCPACGH
MLPWYENVPVLSYLALRGRCSACRARISARYPLVEIVTGGLFFFCIERWGLTAAGIAWCGFSAASMALACIDWDTTLLPD
DITLPLLWAGLLSAALQWIDLPLYDSVLGAVAGYLSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILLS
SVIGAGVGIAMKISSRLREGGYLPFGPFLVGAGLTALVFGPGTMLGLALGLLGL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=267, Percent_Identity=38.2022471910112, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1789732, Length=159, Percent_Identity=46.5408805031447, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31449; Mature: 31449

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGLWLDAALGGVIGLLFGSFLNVVIYRLPKMMERQWAAEFAHRDAPHPAAQAPAVPAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AAPEPFNLLIPRSRCPACGHMLPWYENVPVLSYLALRGRCSACRARISARYPLVEIVTGG
CCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
LFFFCIERWGLTAAGIAWCGFSAASMALACIDWDTTLLPDDITLPLLWAGLLSAALQWID
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LPLYDSVLGAVAGYLSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILLS
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVIGAGVGIAMKISSRLREGGYLPFGPFLVGAGLTALVFGPGTMLGLALGLLGL
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHEEHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLAGLWLDAALGGVIGLLFGSFLNVVIYRLPKMMERQWAAEFAHRDAPHPAAQAPAVPAE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AAPEPFNLLIPRSRCPACGHMLPWYENVPVLSYLALRGRCSACRARISARYPLVEIVTGG
CCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
LFFFCIERWGLTAAGIAWCGFSAASMALACIDWDTTLLPDDITLPLLWAGLLSAALQWID
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
LPLYDSVLGAVAGYLSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILLS
CHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVIGAGVGIAMKISSRLREGGYLPFGPFLVGAGLTALVFGPGTMLGLALGLLGL
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHEEHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]