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The map label for this gene is pgk

Identifier: 120613324

GI number: 120613324

Start: 5236137

End: 5237330

Strand: Direct

Name: pgk

Synonym: Aave_4693

Alternate gene names: 120613324

Gene position: 5236137-5237330 (Clockwise)

Preceding gene: 120613323

Following gene: 120613327

Centisome position: 97.82

GC content: 69.1

Gene sequence:

>1194_bases
ATGCAAATCCTTCGCTTTTCCGACCTCTGCGCCCAGGGCCAGGCCCGTGGCCAGCGCGTGTTCATCCGTGCCGATCTGAA
CGTGCCGCAGGACGACGACGGCCGCATCACCGAAGACACGCGCATCCGCGCATCCGTGCCGTGCATCCGGATGGCGCTGG
ACGCAGGCGCCGCGGTGATGGTGACGAGCCACCTGGGCCGCCCCACGGAAGGCACGCTCACGCCCGCGGACTCGCTCGCG
CCCGTGGCGGCACGCCTGTCGGAACTGCTCGGCTGCGACGTGCCCCTGGTGGCCGACTGGGTGGACGGCGTGCAGGTGCA
GCCCGGCCAGGTCGTGCTGCTGGAGAACTGCCGCGTGAACGTGGGCGAGAAGAAGAACACGCCCGAGCTGGCACAGAAGA
TGGCGAAGCTCTGCGACATCTACGTGAACGACGCCTTCGGCACCGCGCACCGCGCCGAGGGCACGACCTACGGCATCGCC
GAATACGCGAAGGTGGCCTGCGCCGGCCCGCTGCTGTCGGCCGAGATCGATGCCATCGGCAAGGCGCTGTCCCAGCCCGC
GCGGCCGCTGGCCGCCATCGTGGCCGGCAGCAAGGTCTCCACCAAGCTGACCATCCTCAAGAGCCTGTCCGACAAGGTGG
ACCAGCTCATCGTGGGCGGCGGCATCGCCAATACCTTCATGCTGGCCGCGGGCCTTTCGATCGGCAAGAGCCTGGCCGAA
CCCGACCTCGTGAGCGAAGCCAAGGCCGTGATCGAGGCCATGAAGGCGCGCGGCGCCGAGGTGCCGATTCCCACCGACGT
GGTGGTCGCCAAGACCTTCTCGGCCGACGCGCCCGCCACCGTCAAGGCCGCCACCGACGTGGCCGATGACGACCTGATCC
TGGACATCGGCCCGCAGACGGCCGCGAAGCTGGCCGCCCAGCTCAAGGCCGCCGGCACCATCGTCTGGAACGGCCCGGTG
GGCGTGTTCGAGTTCCCCGCCTTCGAGAACGGCACCAAGGCCATCGCCCAGGCGATCGCCGAGTCGCCGGCCTTCAGCAT
CGCCGGCGGCGGCGATACGCTGGCCGCGATCGCGAAATACGGCATCGAGAAGGACGTGGGCTACATCTCCACGGGCGGTG
GCGCCTTCCTGGAAGTGCTGGAAGGCAAGACGCTGCCGGCCTTCGAGATCCTTCACAAGCGCGCCAACGGCTGA

Upstream 100 bases:

>100_bases
GCGACGGCGGCGCCTACAATCCCCCGCAGTTTTTCCCGCCACAGCCCGACCGCGCGCCACGGCAGATGGCCGGCCGGGCT
TTTCCACACCTATCGAGCCC

Downstream 100 bases:

>100_bases
TAGCGGTTTGCGATCGGGTCGCCCTTCAGGGCAACCGAGACAAGAGCCTGCCGGCATCCAGCCTGCGGGCTCTCGTGCCG
TCCGGGGCAGGCGCCGTCAC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQILRFSDLCAQGQARGQRVFIRADLNVPQDDDGRITEDTRIRASVPCIRMALDAGAAVMVTSHLGRPTEGTLTPADSLA
PVAARLSELLGCDVPLVADWVDGVQVQPGQVVLLENCRVNVGEKKNTPELAQKMAKLCDIYVNDAFGTAHRAEGTTYGIA
EYAKVACAGPLLSAEIDAIGKALSQPARPLAAIVAGSKVSTKLTILKSLSDKVDQLIVGGGIANTFMLAAGLSIGKSLAE
PDLVSEAKAVIEAMKARGAEVPIPTDVVVAKTFSADAPATVKAATDVADDDLILDIGPQTAAKLAAQLKAAGTIVWNGPV
GVFEFPAFENGTKAIAQAIAESPAFSIAGGGDTLAAIAKYGIEKDVGYISTGGGAFLEVLEGKTLPAFEILHKRANG

Sequences:

>Translated_397_residues
MQILRFSDLCAQGQARGQRVFIRADLNVPQDDDGRITEDTRIRASVPCIRMALDAGAAVMVTSHLGRPTEGTLTPADSLA
PVAARLSELLGCDVPLVADWVDGVQVQPGQVVLLENCRVNVGEKKNTPELAQKMAKLCDIYVNDAFGTAHRAEGTTYGIA
EYAKVACAGPLLSAEIDAIGKALSQPARPLAAIVAGSKVSTKLTILKSLSDKVDQLIVGGGIANTFMLAAGLSIGKSLAE
PDLVSEAKAVIEAMKARGAEVPIPTDVVVAKTFSADAPATVKAATDVADDDLILDIGPQTAAKLAAQLKAAGTIVWNGPV
GVFEFPAFENGTKAIAQAIAESPAFSIAGGGDTLAAIAKYGIEKDVGYISTGGGAFLEVLEGKTLPAFEILHKRANG
>Mature_397_residues
MQILRFSDLCAQGQARGQRVFIRADLNVPQDDDGRITEDTRIRASVPCIRMALDAGAAVMVTSHLGRPTEGTLTPADSLA
PVAARLSELLGCDVPLVADWVDGVQVQPGQVVLLENCRVNVGEKKNTPELAQKMAKLCDIYVNDAFGTAHRAEGTTYGIA
EYAKVACAGPLLSAEIDAIGKALSQPARPLAAIVAGSKVSTKLTILKSLSDKVDQLIVGGGIANTFMLAAGLSIGKSLAE
PDLVSEAKAVIEAMKARGAEVPIPTDVVVAKTFSADAPATVKAATDVADDDLILDIGPQTAAKLAAQLKAAGTIVWNGPV
GVFEFPAFENGTKAIAQAIAESPAFSIAGGGDTLAAIAKYGIEKDVGYISTGGGAFLEVLEGKTLPAFEILHKRANG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=406, Percent_Identity=40.3940886699507, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI4505763, Length=406, Percent_Identity=39.6551724137931, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=59.2405063291139, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17508823, Length=403, Percent_Identity=43.1761786600496, Blast_Score=290, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=410, Percent_Identity=40.2439024390244, Blast_Score=260, Evalue=3e-70,
Organism=Drosophila melanogaster, GI17136394, Length=400, Percent_Identity=41.5, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24581203, Length=398, Percent_Identity=37.6884422110553, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ACIAC (A1TW90)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_973002.1
- ProteinModelPortal:   A1TW90
- SMR:   A1TW90
- STRING:   A1TW90
- GeneID:   4669169
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_4693
- NMPDR:   fig|397945.5.peg.4048
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- PhylomeDB:   A1TW90
- ProtClustDB:   PRK00073
- BioCyc:   AAVE397945:AAVE_4693-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41083; Mature: 41083

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 41-41 BINDING 118-118 BINDING 151-151 BINDING 202-202 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILRFSDLCAQGQARGQRVFIRADLNVPQDDDGRITEDTRIRASVPCIRMALDAGAAVM
CCEEEHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCEEE
VTSHLGRPTEGTLTPADSLAPVAARLSELLGCDVPLVADWVDGVQVQPGQVVLLENCRVN
EEHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCEECCCCEEEEECCEEC
VGEKKNTPELAQKMAKLCDIYVNDAFGTAHRAEGTTYGIAEYAKVACAGPLLSAEIDAIG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHHCCCHHHHHHHHHH
KALSQPARPLAAIVAGSKVSTKLTILKSLSDKVDQLIVGGGIANTFMLAAGLSIGKSLAE
HHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCC
PDLVSEAKAVIEAMKARGAEVPIPTDVVVAKTFSADAPATVKAATDVADDDLILDIGPQT
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCEEEEECCHH
AAKLAAQLKAAGTIVWNGPVGVFEFPAFENGTKAIAQAIAESPAFSIAGGGDTLAAIAKY
HHHHHHHHHHCCEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
GIEKDVGYISTGGGAFLEVLEGKTLPAFEILHKRANG
CCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQILRFSDLCAQGQARGQRVFIRADLNVPQDDDGRITEDTRIRASVPCIRMALDAGAAVM
CCEEEHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCEEE
VTSHLGRPTEGTLTPADSLAPVAARLSELLGCDVPLVADWVDGVQVQPGQVVLLENCRVN
EEHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCEECCCCEEEEECCEEC
VGEKKNTPELAQKMAKLCDIYVNDAFGTAHRAEGTTYGIAEYAKVACAGPLLSAEIDAIG
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECHHHHHHHHHCCCHHHHHHHHHH
KALSQPARPLAAIVAGSKVSTKLTILKSLSDKVDQLIVGGGIANTFMLAAGLSIGKSLAE
HHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHCC
PDLVSEAKAVIEAMKARGAEVPIPTDVVVAKTFSADAPATVKAATDVADDDLILDIGPQT
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCEEEEECCHH
AAKLAAQLKAAGTIVWNGPVGVFEFPAFENGTKAIAQAIAESPAFSIAGGGDTLAAIAKY
HHHHHHHHHHCCEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
GIEKDVGYISTGGGAFLEVLEGKTLPAFEILHKRANG
CCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA