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The map label for this gene is prpE [H]

Identifier: 120611491

GI number: 120611491

Start: 3105499

End: 3107424

Strand: Reverse

Name: prpE [H]

Synonym: Aave_2827

Alternate gene names: 120611491

Gene position: 3107424-3105499 (Counterclockwise)

Preceding gene: 120611492

Following gene: 120611486

Centisome position: 58.05

GC content: 70.98

Gene sequence:

>1926_bases
ATGGGCCGCTATGCCGATTTCCACCGCCGCTCCCTGCAGGAGCGCGACGCTTTCTGGGCCGAGCAGGCCCGCCTGATCGA
CTGGCAGGAGCCGCCCCGGCGCATCTGCAACTACGACCGGCCGCCATTCGCGCGCTGGTTCGAGGGCGGCACCACCAATC
TCTGCCACAACGCGGTGGACCGGCACCTCGCGCAGCGCGGCGGGCAGCCGGCGCTGATCGCGACCTCGTCCGAAACCGAC
TCCGAGCGCATCTACAGCTTCGCCGAGCTGCATGCGGAAGTGCAGCGCATGGCGGCCGCTTTGCGGTCGCTCGGGGTGCA
GGCGGGGGACCGGGTGCTGATCTACATGCCGATGGTGGCCGAGGCCGCGTTCGCCATGCTGGCCTGCGCCCGCATCGGGG
CGCTGCATTCGGTGGTGTTCGGCGGGTTCGCGTCGGGTGCGCTGGCCTCACGGATCGAGGATGCCGAACCGGTGGCCATC
GTCAGCGCGGACGCGGGTTCGCGCGGGGGCAAGGTGGTGCCCTACAAGCCTTTGCTGGACGAGGCGCTGCAGCTCTCAAA
GCACCAGCCGGGGGCGGTGCTCCTCGTGGACCGGGGGCTGGCCCCGATGGAACTGCGGCCCGGCCGTGACCACCGCTGGG
AGGAACTGCGGGCGCGGCATCTGGACGCGCGGGTGGACTGCGAATGGCTGGATGCCACCCATCCGAGCTATACGCTCTAC
ACCAGCGGCACCACCGGCCGGCCCAAGGGCGTACAGCGGGATACGGGCGGATACGCGGTGGCGCTGGCGGCCAGCATGCG
GCATATCTTCGATGCCGGTGCGCAGGACTCCGGTGGCACGCGGACCCCAGGGGTGTTCTTCGCCACCAGCGACATCGGCT
GGGTGGTGGGGCACAGCTACATCGTGTATGGCCCGCTCATCGCCGGCATGGCGACGGTGATGTACGAGGGCCTGCCGATC
CGTCCGGACGCGGGCGTCTGGTGGCGCATCGTCGAGCGGCACCGGGTGACGCACATGTTCTCTGCGCCCACGGCCGTGCG
CGTGCTGAAGAAGCAGGATCCGGAGTGGCTTCGGCGCTACGACCTGTCGAGCCTGAAGGCCCTGTGGCTGGCGGGCGAGC
CGCTGGACGCCCCCACGGCGCAATGGATCGGCGAGGCGCTGGGCGTGCCGATCGTGGACAACTACTGGCAGACCGAAACC
GGCTGGCCGATCCTCACGCTGGCCAACGGGGTGGAGCCGCAGCCGTCCCGCGCGGGCAGCCCGGGCAAGGCCATGTACGG
CTACGACGTGAAGCTGATCGACCAGGCCACGGGCGAGGAACTGGCGGAGCCGGACCGCAAGGGCGTGCTCGCCATCGAGG
GGCCGCTGCCCCCGGGGTGCATGCAGACCGTGTGGCGCGACGACGAGCGCTTCGTGCAGACGTACTGGAAGAGCATTCCG
GGGCGTCTGGTGTACAGCACGTTCGACTGGGGCGTCCGCGACCGGGACGGCTACTACTTCATCCTGGGACGCACGGACGA
CGTGATCAACGTGGCCGGGCACCGGCTGGGCACGCGGGAGATCGAGGAGGCGATCGCCGCGCATCCGCAGGTGGCGGAGG
TGGCGGTGGTGGGCGTGGCCGATGCGCTCAAGGGCCAGGTGGCGATGGCCTTCGCGGTGGCGCGCGATCCGGCGGCCGTG
GCCGACGCGGCGGACCGCCGGCTGCTGGAAGGAGGTGTGATGCAACTCGTGGACCAGCGGCTCGGCGCGGTGGCCCGGCC
GTCGCGCGTGCATTTCGTGGCGCTGCTGCCCAAGACGCGCAGCGGCAAGCTGCTGCGCCGCGCGCTGCAGGCCGTGGCCG
AGCGCCGCGACCCCGGGGATCTCACCACCATGGAAGATCCCTCGGCGCTGCAGCAGGTGCGGGATCTGCTGGCCGGGACG
GAGTAG

Upstream 100 bases:

>100_bases
GGCGCGGCTGTCAGGATGCCGCAAGCGCGGGGTGAATGATGCTGTCTGCTGAGCCTGCGTGCGAACATGCGCGGGCCATG
ACGACAATCAGGAGACACCC

Downstream 100 bases:

>100_bases
GCGCGAAACGGAGCAGGCACCGCCGCGCCGGCCGGGCGCGGCGAAGAAGCGTCCGGCGGGTGCGAGGCAGCGTCACACCA
CCACGGGCGGACGCCAGGTT

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MGRYADFHRRSLQERDAFWAEQARLIDWQEPPRRICNYDRPPFARWFEGGTTNLCHNAVDRHLAQRGGQPALIATSSETD
SERIYSFAELHAEVQRMAAALRSLGVQAGDRVLIYMPMVAEAAFAMLACARIGALHSVVFGGFASGALASRIEDAEPVAI
VSADAGSRGGKVVPYKPLLDEALQLSKHQPGAVLLVDRGLAPMELRPGRDHRWEELRARHLDARVDCEWLDATHPSYTLY
TSGTTGRPKGVQRDTGGYAVALAASMRHIFDAGAQDSGGTRTPGVFFATSDIGWVVGHSYIVYGPLIAGMATVMYEGLPI
RPDAGVWWRIVERHRVTHMFSAPTAVRVLKKQDPEWLRRYDLSSLKALWLAGEPLDAPTAQWIGEALGVPIVDNYWQTET
GWPILTLANGVEPQPSRAGSPGKAMYGYDVKLIDQATGEELAEPDRKGVLAIEGPLPPGCMQTVWRDDERFVQTYWKSIP
GRLVYSTFDWGVRDRDGYYFILGRTDDVINVAGHRLGTREIEEAIAAHPQVAEVAVVGVADALKGQVAMAFAVARDPAAV
ADAADRRLLEGGVMQLVDQRLGAVARPSRVHFVALLPKTRSGKLLRRALQAVAERRDPGDLTTMEDPSALQQVRDLLAGT
E

Sequences:

>Translated_641_residues
MGRYADFHRRSLQERDAFWAEQARLIDWQEPPRRICNYDRPPFARWFEGGTTNLCHNAVDRHLAQRGGQPALIATSSETD
SERIYSFAELHAEVQRMAAALRSLGVQAGDRVLIYMPMVAEAAFAMLACARIGALHSVVFGGFASGALASRIEDAEPVAI
VSADAGSRGGKVVPYKPLLDEALQLSKHQPGAVLLVDRGLAPMELRPGRDHRWEELRARHLDARVDCEWLDATHPSYTLY
TSGTTGRPKGVQRDTGGYAVALAASMRHIFDAGAQDSGGTRTPGVFFATSDIGWVVGHSYIVYGPLIAGMATVMYEGLPI
RPDAGVWWRIVERHRVTHMFSAPTAVRVLKKQDPEWLRRYDLSSLKALWLAGEPLDAPTAQWIGEALGVPIVDNYWQTET
GWPILTLANGVEPQPSRAGSPGKAMYGYDVKLIDQATGEELAEPDRKGVLAIEGPLPPGCMQTVWRDDERFVQTYWKSIP
GRLVYSTFDWGVRDRDGYYFILGRTDDVINVAGHRLGTREIEEAIAAHPQVAEVAVVGVADALKGQVAMAFAVARDPAAV
ADAADRRLLEGGVMQLVDQRLGAVARPSRVHFVALLPKTRSGKLLRRALQAVAERRDPGDLTTMEDPSALQQVRDLLAGT
E
>Mature_640_residues
GRYADFHRRSLQERDAFWAEQARLIDWQEPPRRICNYDRPPFARWFEGGTTNLCHNAVDRHLAQRGGQPALIATSSETDS
ERIYSFAELHAEVQRMAAALRSLGVQAGDRVLIYMPMVAEAAFAMLACARIGALHSVVFGGFASGALASRIEDAEPVAIV
SADAGSRGGKVVPYKPLLDEALQLSKHQPGAVLLVDRGLAPMELRPGRDHRWEELRARHLDARVDCEWLDATHPSYTLYT
SGTTGRPKGVQRDTGGYAVALAASMRHIFDAGAQDSGGTRTPGVFFATSDIGWVVGHSYIVYGPLIAGMATVMYEGLPIR
PDAGVWWRIVERHRVTHMFSAPTAVRVLKKQDPEWLRRYDLSSLKALWLAGEPLDAPTAQWIGEALGVPIVDNYWQTETG
WPILTLANGVEPQPSRAGSPGKAMYGYDVKLIDQATGEELAEPDRKGVLAIEGPLPPGCMQTVWRDDERFVQTYWKSIPG
RLVYSTFDWGVRDRDGYYFILGRTDDVINVAGHRLGTREIEEAIAAHPQVAEVAVVGVADALKGQVAMAFAVARDPAAVA
DAADRRLLEGGVMQLVDQRLGAVARPSRVHFVALLPKTRSGKLLRRALQAVAERRDPGDLTTMEDPSALQQVRDLLAGTE

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=644, Percent_Identity=41.1490683229814, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI8923896, Length=670, Percent_Identity=37.3134328358209, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI253970489, Length=683, Percent_Identity=36.6032210834553, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI28416953, Length=648, Percent_Identity=37.6543209876543, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI38505220, Length=568, Percent_Identity=28.5211267605634, Blast_Score=176, Evalue=8e-44,
Organism=Homo sapiens, GI122937307, Length=550, Percent_Identity=28, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=27.8504672897196, Blast_Score=163, Evalue=6e-40,
Organism=Homo sapiens, GI115511026, Length=551, Percent_Identity=27.7676950998185, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI58082049, Length=532, Percent_Identity=27.4436090225564, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI157311624, Length=535, Percent_Identity=26.9158878504673, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI157311622, Length=535, Percent_Identity=26.9158878504673, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI31982927, Length=667, Percent_Identity=23.688155922039, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI195947378, Length=468, Percent_Identity=22.4358974358974, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI187761345, Length=402, Percent_Identity=25.1243781094527, Blast_Score=74, Evalue=6e-13,
Organism=Homo sapiens, GI187761343, Length=402, Percent_Identity=25.1243781094527, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1786529, Length=634, Percent_Identity=60.8832807570978, Blast_Score=782, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=644, Percent_Identity=38.1987577639752, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI221142682, Length=558, Percent_Identity=23.2974910394265, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=23.8805970149254, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788107, Length=574, Percent_Identity=24.0418118466899, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI145693145, Length=395, Percent_Identity=25.5696202531646, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1786801, Length=470, Percent_Identity=23.1914893617021, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=656, Percent_Identity=36.1280487804878, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71982997, Length=656, Percent_Identity=36.1280487804878, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17569507, Length=650, Percent_Identity=21.3846153846154, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=556, Percent_Identity=24.8201438848921, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=567, Percent_Identity=20.9876543209877, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=626, Percent_Identity=22.2044728434505, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=574, Percent_Identity=24.390243902439, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=567, Percent_Identity=23.8095238095238, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=572, Percent_Identity=22.5524475524476, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=651, Percent_Identity=38.5560675883257, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323182, Length=658, Percent_Identity=38.2978723404255, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319699, Length=328, Percent_Identity=22.5609756097561, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355225, Length=644, Percent_Identity=40.2173913043478, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24667955, Length=662, Percent_Identity=35.3474320241692, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI62472339, Length=592, Percent_Identity=35.6418918918919, Blast_Score=352, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24667959, Length=538, Percent_Identity=35.8736059479554, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21355181, Length=548, Percent_Identity=24.6350364963504, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648260, Length=385, Percent_Identity=27.5324675324675, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=384, Percent_Identity=27.34375, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20130357, Length=407, Percent_Identity=25.5528255528256, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 70421; Mature: 70290

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRYADFHRRSLQERDAFWAEQARLIDWQEPPRRICNYDRPPFARWFEGGTTNLCHNAVD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHCCCCHHHHHHHHH
RHLAQRGGQPALIATSSETDSERIYSFAELHAEVQRMAAALRSLGVQAGDRVLIYMPMVA
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHH
EAAFAMLACARIGALHSVVFGGFASGALASRIEDAEPVAIVSADAGSRGGKVVPYKPLLD
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCHHHHH
EALQLSKHQPGAVLLVDRGLAPMELRPGRDHRWEELRARHLDARVDCEWLDATHPSYTLY
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCEEEE
TSGTTGRPKGVQRDTGGYAVALAASMRHIFDAGAQDSGGTRTPGVFFATSDIGWVVGHSY
ECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHCCCH
IVYGPLIAGMATVMYEGLPIRPDAGVWWRIVERHRVTHMFSAPTAVRVLKKQDPEWLRRY
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHH
DLSSLKALWLAGEPLDAPTAQWIGEALGVPIVDNYWQTETGWPILTLANGVEPQPSRAGS
CHHHHHEEEECCCCCCCCHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCC
PGKAMYGYDVKLIDQATGEELAEPDRKGVLAIEGPLPPGCMQTVWRDDERFVQTYWKSIP
CCCEEECCCEEEECCCCCHHHCCCCCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCC
GRLVYSTFDWGVRDRDGYYFILGRTDDVINVAGHRLGTREIEEAIAAHPQVAEVAVVGVA
CCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHH
DALKGQVAMAFAVARDPAAVADAADRRLLEGGVMQLVDQRLGAVARPSRVHFVALLPKTR
HHHCCHHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
SGKLLRRALQAVAERRDPGDLTTMEDPSALQQVRDLLAGTE
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GRYADFHRRSLQERDAFWAEQARLIDWQEPPRRICNYDRPPFARWFEGGTTNLCHNAVD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHCCCCHHHHHHHHH
RHLAQRGGQPALIATSSETDSERIYSFAELHAEVQRMAAALRSLGVQAGDRVLIYMPMVA
HHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHH
EAAFAMLACARIGALHSVVFGGFASGALASRIEDAEPVAIVSADAGSRGGKVVPYKPLLD
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCHHHHH
EALQLSKHQPGAVLLVDRGLAPMELRPGRDHRWEELRARHLDARVDCEWLDATHPSYTLY
HHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCEEEE
TSGTTGRPKGVQRDTGGYAVALAASMRHIFDAGAQDSGGTRTPGVFFATSDIGWVVGHSY
ECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHCCCH
IVYGPLIAGMATVMYEGLPIRPDAGVWWRIVERHRVTHMFSAPTAVRVLKKQDPEWLRRY
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHH
DLSSLKALWLAGEPLDAPTAQWIGEALGVPIVDNYWQTETGWPILTLANGVEPQPSRAGS
CHHHHHEEEECCCCCCCCHHHHHHHHCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCC
PGKAMYGYDVKLIDQATGEELAEPDRKGVLAIEGPLPPGCMQTVWRDDERFVQTYWKSIP
CCCEEECCCEEEECCCCCHHHCCCCCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCC
GRLVYSTFDWGVRDRDGYYFILGRTDDVINVAGHRLGTREIEEAIAAHPQVAEVAVVGVA
CCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEEHH
DALKGQVAMAFAVARDPAAVADAADRRLLEGGVMQLVDQRLGAVARPSRVHFVALLPKTR
HHHCCHHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
SGKLLRRALQAVAERRDPGDLTTMEDPSALQQVRDLLAGTE
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9006051; 10411265; 11677609; 10482501 [H]