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The map label for this gene is btuB [H]

Identifier: 120610222

GI number: 120610222

Start: 1702661

End: 1704442

Strand: Direct

Name: btuB [H]

Synonym: Aave_1537

Alternate gene names: 120610222

Gene position: 1702661-1704442 (Clockwise)

Preceding gene: 120610219

Following gene: 120610223

Centisome position: 31.81

GC content: 72.05

Gene sequence:

>1782_bases
ATGGGCCTGTCGCTGGCCCCGGCGGCGCATGCCGCCACCGCCGCCCCCATGGAAGAGGTCGTGGTGACCGCCAACCGCAC
GCCCCAGCCCCTGGCCGATCTCGTGGCCGACGTCTCCATCGTGGACCGCGACACCATCGAGGCCAGCGGCGCCACGGGGC
TGGCCGATGTGCTGGCCCGCGTGCCGGGCATCGAGATTTCACGCAACGGCGGACCGGGCACGACGACCAGCGTCTATCTG
CGCGGCGCGGAGCAGCGCTTCACCGCCGTCTATATCGACGGCGTGCGGATGGATTCGCAGACCACGGGCGGCGCGCCCTG
GGAAGCGATCCCGCTGGGCCTCATCGACCGCATCGAGGTGCTGCGCGGCCCCGCCGCCGCCGTGTACGGATCGGACGCCG
TGGGCGGCGTGATCCAGATCTTCACGCGCAAGGGCGAGGGCCCGGCGCAGCCGTACGTCGGCATCGGCGCCGGCAGCCAC
GGCACCTACAAGGCCGAGGCCGGCGTGAGCGGCTCGGCGGGCGAGGGCCGCGCGGTGGACTACGCGCTGGGCCTGGAGCG
CGAGATCAGCGACGGCTTCAATGCCCGGCCCGTCGCGGGCCAGAACCCCGACGAGGACGGCCTGCGCCGCTCCGCCGTGA
ACGCGCGCGTGGGCCTGCAGATCGATCCGCACCAGCGCCTCGAAGGCACGCTGCTGGGGGCCGACACCAACGCGGGCTAC
GACACCACGCCCCTGGGCAACGACGACCGCGCCCTGCACCGCATGCATGCGCTGGGCCTGAACTGGCGCGCGCAATGGAG
CGACCGCTACACCACCCGCGTATCGGTCACGGATTCGCGCGAGCGCTACGAGACGCGTCCCTCGCCCTACACCGCCGATA
CGCGCCTGCGGGGCTACCTGTTCCAGAACGAGTGGCGCGAGGGGGCGCACTTCTTCACCGCCGCGCTGGAGCGCCGCGAG
GACCAGCTGCGCAACGGGGACATCGATGGCGACCGCTCGCAGGACGCGCTGGCGCTGGGCTATGGCTTCACCGCCGGCCC
GCATGCGCTGCAGATCAACGCACGCCACGATTCGGACAGCGAGTTCGGTGGGCACAACACCGGCAGCATCGCCTACGGCT
ACGCGATCACGCCGCAGTGGCGCGCCACGGCATCGGCCGGCACCTCCTTCCGCGCGCCCACGCTCTACCAGCGCTTCAGT
CCCTACGGCGTCGCGTCGCTGAAGCCGGAAGAGGGCCGCAACGTGGAGCTGGGCCTGCGCTGGGCGCAAGGCGCGAGCAG
CGCGTCCGTGGTGGCCTACCGCAACCGCGTGAAGAACCTCATCGTCTTCGGCGAGGCCGGTCCGTGCCAGGATGAGTTCG
GCTGCTATGCCAACGCGGGCCGGGCGAAGTACGAGGGCGTGACGCTCTCGGCACGGCACACCCTGGGCGGCGTGCAGTTG
CGCGCATCGGTCGATCTGCAGAACCACCGCGACGAAACCACCGGCCGCCAGTTGCCCCGCCGCGCCAGGCGCCACGCGAC
CCTGGGCGCCGACACGCGCATCGCCGGCTGGACGGTGGGCTCCGAGGTGCAGGCCGCGGGCCGCCGCTTCGACAACGCCG
CCAACACGGCGGTGCTGGGCGGCTACACGCTCGTGAACCTGTACGCCAGCACGCGTGTGGCGCAGGACACGACGCTGCTC
GCGCGCGTGGACAACCTCGCCGACAGGAACTACCAGACCGCGCGCACCTATGCGCAGGCGGGCCGCACGTTCTATGTGGG
CCTGAAGTGGGCGCCGCGTTGA

Upstream 100 bases:

>100_bases
CGCTTCCATGCATTCTTCGATCCCCCGGTTCCGCGGCGCCGCCGGCGCGCGTGCGGCCCTTGTCCCCCTCCACGCGCCCG
CCGCACTTGTCCTGTTCGCC

Downstream 100 bases:

>100_bases
GCGGGGCCGCAGGCACCGCGGGCAGCATTCGCTGTCCGGCGATCGCGATCGCCGCGCCGTCGTCGGGGCAGGGCAAGACC
ACCGTGACCGCCGCGCTGGC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MGLSLAPAAHAATAAPMEEVVVTANRTPQPLADLVADVSIVDRDTIEASGATGLADVLARVPGIEISRNGGPGTTTSVYL
RGAEQRFTAVYIDGVRMDSQTTGGAPWEAIPLGLIDRIEVLRGPAAAVYGSDAVGGVIQIFTRKGEGPAQPYVGIGAGSH
GTYKAEAGVSGSAGEGRAVDYALGLEREISDGFNARPVAGQNPDEDGLRRSAVNARVGLQIDPHQRLEGTLLGADTNAGY
DTTPLGNDDRALHRMHALGLNWRAQWSDRYTTRVSVTDSRERYETRPSPYTADTRLRGYLFQNEWREGAHFFTAALERRE
DQLRNGDIDGDRSQDALALGYGFTAGPHALQINARHDSDSEFGGHNTGSIAYGYAITPQWRATASAGTSFRAPTLYQRFS
PYGVASLKPEEGRNVELGLRWAQGASSASVVAYRNRVKNLIVFGEAGPCQDEFGCYANAGRAKYEGVTLSARHTLGGVQL
RASVDLQNHRDETTGRQLPRRARRHATLGADTRIAGWTVGSEVQAAGRRFDNAANTAVLGGYTLVNLYASTRVAQDTTLL
ARVDNLADRNYQTARTYAQAGRTFYVGLKWAPR

Sequences:

>Translated_593_residues
MGLSLAPAAHAATAAPMEEVVVTANRTPQPLADLVADVSIVDRDTIEASGATGLADVLARVPGIEISRNGGPGTTTSVYL
RGAEQRFTAVYIDGVRMDSQTTGGAPWEAIPLGLIDRIEVLRGPAAAVYGSDAVGGVIQIFTRKGEGPAQPYVGIGAGSH
GTYKAEAGVSGSAGEGRAVDYALGLEREISDGFNARPVAGQNPDEDGLRRSAVNARVGLQIDPHQRLEGTLLGADTNAGY
DTTPLGNDDRALHRMHALGLNWRAQWSDRYTTRVSVTDSRERYETRPSPYTADTRLRGYLFQNEWREGAHFFTAALERRE
DQLRNGDIDGDRSQDALALGYGFTAGPHALQINARHDSDSEFGGHNTGSIAYGYAITPQWRATASAGTSFRAPTLYQRFS
PYGVASLKPEEGRNVELGLRWAQGASSASVVAYRNRVKNLIVFGEAGPCQDEFGCYANAGRAKYEGVTLSARHTLGGVQL
RASVDLQNHRDETTGRQLPRRARRHATLGADTRIAGWTVGSEVQAAGRRFDNAANTAVLGGYTLVNLYASTRVAQDTTLL
ARVDNLADRNYQTARTYAQAGRTFYVGLKWAPR
>Mature_592_residues
GLSLAPAAHAATAAPMEEVVVTANRTPQPLADLVADVSIVDRDTIEASGATGLADVLARVPGIEISRNGGPGTTTSVYLR
GAEQRFTAVYIDGVRMDSQTTGGAPWEAIPLGLIDRIEVLRGPAAAVYGSDAVGGVIQIFTRKGEGPAQPYVGIGAGSHG
TYKAEAGVSGSAGEGRAVDYALGLEREISDGFNARPVAGQNPDEDGLRRSAVNARVGLQIDPHQRLEGTLLGADTNAGYD
TTPLGNDDRALHRMHALGLNWRAQWSDRYTTRVSVTDSRERYETRPSPYTADTRLRGYLFQNEWREGAHFFTAALERRED
QLRNGDIDGDRSQDALALGYGFTAGPHALQINARHDSDSEFGGHNTGSIAYGYAITPQWRATASAGTSFRAPTLYQRFSP
YGVASLKPEEGRNVELGLRWAQGASSASVVAYRNRVKNLIVFGEAGPCQDEFGCYANAGRAKYEGVTLSARHTLGGVQLR
ASVDLQNHRDETTGRQLPRRARRHATLGADTRIAGWTVGSEVQAAGRRFDNAANTAVLGGYTLVNLYASTRVAQDTTLLA
RVDNLADRNYQTARTYAQAGRTFYVGLKWAPR

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=675, Percent_Identity=24.4444444444444, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1790405, Length=159, Percent_Identity=39.622641509434, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787723, Length=248, Percent_Identity=27.0161290322581, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1786798, Length=708, Percent_Identity=21.6101694915254, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 63698; Mature: 63567

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSLAPAAHAATAAPMEEVVVTANRTPQPLADLVADVSIVDRDTIEASGATGLADVLAR
CCCCCCCCHHHHHHCCHHHEEEECCCCCHHHHHHHHCCHHEECCCCCCCCCCCHHHHHHH
VPGIEISRNGGPGTTTSVYLRGAEQRFTAVYIDGVRMDSQTTGGAPWEAIPLGLIDRIEV
CCCCEEECCCCCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCHHCCHHHHHHHHH
LRGPAAAVYGSDAVGGVIQIFTRKGEGPAQPYVGIGAGSHGTYKAEAGVSGSAGEGRAVD
HCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCEEE
YALGLEREISDGFNARPVAGQNPDEDGLRRSAVNARVGLQIDPHQRLEGTLLGADTNAGY
EEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCC
DTTPLGNDDRALHRMHALGLNWRAQWSDRYTTRVSVTDSRERYETRPSPYTADTRLRGYL
CCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEECCCHHHHCCCCCCCCCCCCEEEEE
FQNEWREGAHFFTAALERREDQLRNGDIDGDRSQDALALGYGFTAGPHALQINARHDSDS
EHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCC
EFGGHNTGSIAYGYAITPQWRATASAGTSFRAPTLYQRFSPYGVASLKPEEGRNVELGLR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCEEEEEE
WAQGASSASVVAYRNRVKNLIVFGEAGPCQDEFGCYANAGRAKYEGVTLSARHTLGGVQL
EECCCCCCHHHHHHHCCCEEEEECCCCCCHHHCCCEECCCCCEECCEEEEECCCCCCEEE
RASVDLQNHRDETTGRQLPRRARRHATLGADTRIAGWTVGSEVQAAGRRFDNAANTAVLG
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEC
GYTLVNLYASTRVAQDTTLLARVDNLADRNYQTARTYAQAGRTFYVGLKWAPR
CEEEEEEHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCC
>Mature Secondary Structure 
GLSLAPAAHAATAAPMEEVVVTANRTPQPLADLVADVSIVDRDTIEASGATGLADVLAR
CCCCCCCHHHHHHCCHHHEEEECCCCCHHHHHHHHCCHHEECCCCCCCCCCCHHHHHHH
VPGIEISRNGGPGTTTSVYLRGAEQRFTAVYIDGVRMDSQTTGGAPWEAIPLGLIDRIEV
CCCCEEECCCCCCCCEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCHHCCHHHHHHHHH
LRGPAAAVYGSDAVGGVIQIFTRKGEGPAQPYVGIGAGSHGTYKAEAGVSGSAGEGRAVD
HCCCCCEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCEEE
YALGLEREISDGFNARPVAGQNPDEDGLRRSAVNARVGLQIDPHQRLEGTLLGADTNAGY
EEECCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCC
DTTPLGNDDRALHRMHALGLNWRAQWSDRYTTRVSVTDSRERYETRPSPYTADTRLRGYL
CCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEECCCHHHHCCCCCCCCCCCCEEEEE
FQNEWREGAHFFTAALERREDQLRNGDIDGDRSQDALALGYGFTAGPHALQINARHDSDS
EHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCC
EFGGHNTGSIAYGYAITPQWRATASAGTSFRAPTLYQRFSPYGVASLKPEEGRNVELGLR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCEEEEEE
WAQGASSASVVAYRNRVKNLIVFGEAGPCQDEFGCYANAGRAKYEGVTLSARHTLGGVQL
EECCCCCCHHHHHHHCCCEEEEECCCCCCHHHCCCEECCCCCEECCEEEEECCCCCCEEE
RASVDLQNHRDETTGRQLPRRARRHATLGADTRIAGWTVGSEVQAAGRRFDNAANTAVLG
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEC
GYTLVNLYASTRVAQDTTLLARVDNLADRNYQTARTYAQAGRTFYVGLKWAPR
CEEEEEEHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA