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The map label for this gene is cobB [H]

Identifier: 120610223

GI number: 120610223

Start: 1704439

End: 1705863

Strand: Direct

Name: cobB [H]

Synonym: Aave_1538

Alternate gene names: 120610223

Gene position: 1704439-1705863 (Clockwise)

Preceding gene: 120610222

Following gene: 120610224

Centisome position: 31.84

GC content: 76.0

Gene sequence:

>1425_bases
TTGAGCGGGGCCGCAGGCACCGCGGGCAGCATTCGCTGTCCGGCGATCGCGATCGCCGCGCCGTCGTCGGGGCAGGGCAA
GACCACCGTGACCGCCGCGCTGGCACGCCTGCACGCGCGGCAGGGGCGGCGCGTGCGCGTGTTCAAGTGCGGGCCGGACT
TTCTCGATCCCTGCTGGCTGGAGCAGGCCAGCGGCGCGCCGGTGCATGCCATCGATCTCTGGATGACCGGCGAGCAGGAT
GCGGCCGAACGGCTGCACGCCGCGGCGCAGGAGGCCGACCTGATCCTGGTCGAGGGCGTGATGGGCCTTTTCGACGGCAC
GCCCAGCCTCGCGGACCTCGCCCGGCGCTTCGGCCTGCCGGTGCTGGCCGTGGTGGATGCCTCCGCCATGGCGGGCACCT
TCGGCGCGCTGGCCTGGGGCCTGCGGAACTACCGTCCGGGCCTGCCGTGGGCGGGCGTGCTGGCCAACCGCGTGGGCAGC
GCGCGCCATGCCGCCATGCTGCAGGAGAGCCTGGCGGACGCGGGCGACTGGATGGGCGCCCTGGCGCGCGTCGCCCCGCC
CGCGGCGGATGTACCCGCGGGCGACGCGCCACCGGGCCGCCAGCGCGGGGGGCTGCTGCCCGAGCGGCACCTGGGCCTGG
TGGCAGCGCATGAGCTGGCCGACGGCCTGGCGCGCATCGACCTCGCGGCCGATGCGCTGGCGCAGACGCCCCTGGGCCGC
CTGTCTCCGCAGGAATGGCAGGAGCGGTTCGCGGTGGAGTTCGCCGCGCCCGCGCCGCGCGCCGCGGTGCCGCCGCTGCT
GGCCGGCCGCACCGTGGCGGTGGCCGGCGATGCCGCGTTCTGCTTCGTCTATCGGGCCAACCTGCAGACGCTGGAAGCCC
TGGGCGCGCGCGTGCGGCGCTTCTCGCCGCTGCAGGACGCTCAACTGCCGGCCTGCGACGCCGTGTGGCTGCCCGGCGGC
TATCCGGAACTGCACGCCGACCGCCTGGCCGCCAACACCGGCATGCAGCGCAGCCTGCGGGCCCACGTGGAGGCCGGCCG
GCCCGTATGGGCAGAATGCGGCGGCATGATGGCGCTCTTCGAATCGATCACCCTCGCGGACGGCGAACGCAAGCCGCTCT
GGGGGCTGCTGCCGGGCCACGTGGCCATGCAGCGGCGGCTGGCGGCGCTGGGGCCGCAGCAGCTGGACCTGGACGGCCAC
GTGCTGCGCGGCCACACCTTCCATTACTCGACCTGCGAGAGCGGCGCCGCCGTGCGCGCGCGCACCCGCCGCCCGGATCC
GGGCGAGGGCGGCGCGGGCGAGGCGCTCTACCGGCAGGGCAGCATCCACGCGAGCTATTTCCACGCCTGGTTCCCCTCCA
GCCCGCGGGCCGTGGCGGCGCTGTTCGGCGCCGAGGACGCGCTGGCGCCGGAGGCCGCGGCATGA

Upstream 100 bases:

>100_bases
TCGCGCGCGTGGACAACCTCGCCGACAGGAACTACCAGACCGCGCGCACCTATGCGCAGGCGGGCCGCACGTTCTATGTG
GGCCTGAAGTGGGCGCCGCG

Downstream 100 bases:

>100_bases
CGGCCCTGGCGGCCCCTTTCGTGGGCGATGGCGTCGGCGACGGCATGCAGGGCGCGCCGCTGTCGGTCGCGCGCAGCGAA
TTCATCCTGGGCGGGCAGAA

Product: cobyrinate a,c-diamide synthase / hydrogenobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSGAAGTAGSIRCPAIAIAAPSSGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPCWLEQASGAPVHAIDLWMTGEQD
AAERLHAAAQEADLILVEGVMGLFDGTPSLADLARRFGLPVLAVVDASAMAGTFGALAWGLRNYRPGLPWAGVLANRVGS
ARHAAMLQESLADAGDWMGALARVAPPAADVPAGDAPPGRQRGGLLPERHLGLVAAHELADGLARIDLAADALAQTPLGR
LSPQEWQERFAVEFAAPAPRAAVPPLLAGRTVAVAGDAAFCFVYRANLQTLEALGARVRRFSPLQDAQLPACDAVWLPGG
YPELHADRLAANTGMQRSLRAHVEAGRPVWAECGGMMALFESITLADGERKPLWGLLPGHVAMQRRLAALGPQQLDLDGH
VLRGHTFHYSTCESGAAVRARTRRPDPGEGGAGEALYRQGSIHASYFHAWFPSSPRAVAALFGAEDALAPEAAA

Sequences:

>Translated_474_residues
MSGAAGTAGSIRCPAIAIAAPSSGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPCWLEQASGAPVHAIDLWMTGEQD
AAERLHAAAQEADLILVEGVMGLFDGTPSLADLARRFGLPVLAVVDASAMAGTFGALAWGLRNYRPGLPWAGVLANRVGS
ARHAAMLQESLADAGDWMGALARVAPPAADVPAGDAPPGRQRGGLLPERHLGLVAAHELADGLARIDLAADALAQTPLGR
LSPQEWQERFAVEFAAPAPRAAVPPLLAGRTVAVAGDAAFCFVYRANLQTLEALGARVRRFSPLQDAQLPACDAVWLPGG
YPELHADRLAANTGMQRSLRAHVEAGRPVWAECGGMMALFESITLADGERKPLWGLLPGHVAMQRRLAALGPQQLDLDGH
VLRGHTFHYSTCESGAAVRARTRRPDPGEGGAGEALYRQGSIHASYFHAWFPSSPRAVAALFGAEDALAPEAAA
>Mature_473_residues
SGAAGTAGSIRCPAIAIAAPSSGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPCWLEQASGAPVHAIDLWMTGEQDA
AERLHAAAQEADLILVEGVMGLFDGTPSLADLARRFGLPVLAVVDASAMAGTFGALAWGLRNYRPGLPWAGVLANRVGSA
RHAAMLQESLADAGDWMGALARVAPPAADVPAGDAPPGRQRGGLLPERHLGLVAAHELADGLARIDLAADALAQTPLGRL
SPQEWQERFAVEFAAPAPRAAVPPLLAGRTVAVAGDAAFCFVYRANLQTLEALGARVRRFSPLQDAQLPACDAVWLPGGY
PELHADRLAANTGMQRSLRAHVEAGRPVWAECGGMMALFESITLADGERKPLWGLLPGHVAMQRRLAALGPQQLDLDGHV
LRGHTFHYSTCESGAAVRARTRRPDPGEGGAGEALYRQGSIHASYFHAWFPSSPRAVAALFGAEDALAPEAAA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 49718; Mature: 49587

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAAGTAGSIRCPAIAIAAPSSGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPCWL
CCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
EQASGAPVHAIDLWMTGEQDAAERLHAAAQEADLILVEGVMGLFDGTPSLADLARRFGLP
HHCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCHHHHHHHHHCCC
VLAVVDASAMAGTFGALAWGLRNYRPGLPWAGVLANRVGSARHAAMLQESLADAGDWMGA
EEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LARVAPPAADVPAGDAPPGRQRGGLLPERHLGLVAAHELADGLARIDLAADALAQTPLGR
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSPQEWQERFAVEFAAPAPRAAVPPLLAGRTVAVAGDAAFCFVYRANLQTLEALGARVRR
CCHHHHHHHHEEEECCCCCCCCCCCHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHH
FSPLQDAQLPACDAVWLPGGYPELHADRLAANTGMQRSLRAHVEAGRPVWAECGGMMALF
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCHHHHHH
ESITLADGERKPLWGLLPGHVAMQRRLAALGPQQLDLDGHVLRGHTFHYSTCESGAAVRA
HHHHHCCCCCCCEECCCCHHHHHHHHHHHCCCCEECCCCEEEECCEEEECCCCCCCCEEE
RTRRPDPGEGGAGEALYRQGSIHASYFHAWFPSSPRAVAALFGAEDALAPEAAA
CCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
SGAAGTAGSIRCPAIAIAAPSSGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPCWL
CCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH
EQASGAPVHAIDLWMTGEQDAAERLHAAAQEADLILVEGVMGLFDGTPSLADLARRFGLP
HHCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCHHHHHHHHHCCC
VLAVVDASAMAGTFGALAWGLRNYRPGLPWAGVLANRVGSARHAAMLQESLADAGDWMGA
EEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LARVAPPAADVPAGDAPPGRQRGGLLPERHLGLVAAHELADGLARIDLAADALAQTPLGR
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSPQEWQERFAVEFAAPAPRAAVPPLLAGRTVAVAGDAAFCFVYRANLQTLEALGARVRR
CCHHHHHHHHEEEECCCCCCCCCCCHHCCCEEEEECCCEEEEEEECCHHHHHHHHHHHHH
FSPLQDAQLPACDAVWLPGGYPELHADRLAANTGMQRSLRAHVEAGRPVWAECGGMMALF
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCHHHHHH
ESITLADGERKPLWGLLPGHVAMQRRLAALGPQQLDLDGHVLRGHTFHYSTCESGAAVRA
HHHHHCCCCCCCEECCCCHHHHHHHHHHHCCCCEECCCCEEEECCEEEECCCCCCCCEEE
RTRRPDPGEGGAGEALYRQGSIHASYFHAWFPSSPRAVAALFGAEDALAPEAAA
CCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA