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The map label for this gene is narK [H]

Identifier: 120609358

GI number: 120609358

Start: 713593

End: 714879

Strand: Direct

Name: narK [H]

Synonym: Aave_0659

Alternate gene names: 120609358

Gene position: 713593-714879 (Clockwise)

Preceding gene: 120609357

Following gene: 120609359

Centisome position: 13.33

GC content: 67.75

Gene sequence:

>1287_bases
ATGGCAGACACCGCGTCCGCGACCCCGGACGGAACCCCGCGCCGCACGCGCCAGGCCTGGTCCGTGCTCGCCGTGAGCAC
GCTCGCCTTCACCGTGTGCTTCATGGTGTGGATGATGTTCGGCGTCATCGGCATCCCGATCAAGAAGATGCTGGGCCTGA
ACGCCACGCAGTTCGGCCTGCTCACGGCCATGCCGGTGCTCACGGGCTCGCTCGTGCGCGTGCCGCTCGGCATCTGGACG
GACCGCTACGGCGGCCGCATCGTGATGGCCTGCCTGATGGCGGCGACGGTGCCGGCCATCTGGCTCATGGGGTATGCCAC
GCAGTACTGGCACTTCCTCGCGATCGGCCTGTTCGTCGGGCTGGCCGGGGGCTCGTTCTCGGTGGGAACGCCCTACGTGG
CGCGCTGGTTCCCGAAGGAGCGCCAGGGCACGGCCATGGGCATCTACGGTGCCGGCAATTCCGGCGCGGCGGTGAACAAG
TTCGTGGCGCCGGCCATCCTGGTGGCCTTCGGCTGGGCGGCCGTGCCCCATGCCTATGCGGCCATCATGCTCGGCACGCT
GGTGCTGTTCTGGCTGTTCAGCCACAGCGATCCTTCGCACCGCGTGCCCAGCCACGTCACGTTCATGGACCAGTTGCAGG
CCCTCAAGGACCCGCGCGTGCTCAAGTACTGCCAGTACTACAGCATCGTGTTCGGCGGCTACGTGGCGCTGTCGCTCTGG
ATGGTGCAGTACTACGTGGGCGAATACAGCCTGGACATCCGCGTCGCCGCGCTGCTGGCCGCCTGCTTCTCGCTGCCCGG
CGGCGTGCTGCGCGCCATCGGCGGCGCGCTCAGCGACAAATACGGTGCCCACAGCGTGACCTGGTGGGTGCTGTGGGTCT
GCTGGGTGTGCCTGTTCCTGCTGTCGTACCCGCAGACCGATTTCACGGTGCTCGCCGCGGACGGCCCGCGCAGCTTCCAC
ATCGGCCTGAACGTGTATGCGTTCACCGCACTCATGTTCGTGCTGGGCATCGCCATGGCCTTCGGCAAGGCGAGCGTCTT
CAAGTACATCAGCGACGACTACCCCGCCAACATCGGTGCCATCAGCGGCATCGTGGGCCTGGCCGGCGGCCTGGGCGGCT
TCGTGCTGCCGATCCTGTTCGGCGTGCTGCTGGACTGGACGGGCGTGCGCTCCAGCGCCTTCATGCTGATGTACGGCGTG
GTCTGGGTGTCGCTGGCCTGGATGTATTTCACCGAGGTGCGCCGCACCGACGTGATGGACGCCGGCCGCGGCGCGGCGGA
CCGTTGA

Upstream 100 bases:

>100_bases
GGCTAGTTCTTCTGCCTCGCCCCGGGCGTACCACCGGCGGATATGCAGAGCGCGCCGCCGGAGCGATCATGCATGCCATC
GCTCCCACGAGGAAAACAAG

Downstream 100 bases:

>100_bases
ATCCCCAATGCAAGGAGACCGTGACATGCCTGCCACCCCTTCTTCCTCTTCTTCCTCTCCCTCCCGCGGCGGTGCCCGGG
CCGGGCGCGTGCTGACCCTG

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MADTASATPDGTPRRTRQAWSVLAVSTLAFTVCFMVWMMFGVIGIPIKKMLGLNATQFGLLTAMPVLTGSLVRVPLGIWT
DRYGGRIVMACLMAATVPAIWLMGYATQYWHFLAIGLFVGLAGGSFSVGTPYVARWFPKERQGTAMGIYGAGNSGAAVNK
FVAPAILVAFGWAAVPHAYAAIMLGTLVLFWLFSHSDPSHRVPSHVTFMDQLQALKDPRVLKYCQYYSIVFGGYVALSLW
MVQYYVGEYSLDIRVAALLAACFSLPGGVLRAIGGALSDKYGAHSVTWWVLWVCWVCLFLLSYPQTDFTVLAADGPRSFH
IGLNVYAFTALMFVLGIAMAFGKASVFKYISDDYPANIGAISGIVGLAGGLGGFVLPILFGVLLDWTGVRSSAFMLMYGV
VWVSLAWMYFTEVRRTDVMDAGRGAADR

Sequences:

>Translated_428_residues
MADTASATPDGTPRRTRQAWSVLAVSTLAFTVCFMVWMMFGVIGIPIKKMLGLNATQFGLLTAMPVLTGSLVRVPLGIWT
DRYGGRIVMACLMAATVPAIWLMGYATQYWHFLAIGLFVGLAGGSFSVGTPYVARWFPKERQGTAMGIYGAGNSGAAVNK
FVAPAILVAFGWAAVPHAYAAIMLGTLVLFWLFSHSDPSHRVPSHVTFMDQLQALKDPRVLKYCQYYSIVFGGYVALSLW
MVQYYVGEYSLDIRVAALLAACFSLPGGVLRAIGGALSDKYGAHSVTWWVLWVCWVCLFLLSYPQTDFTVLAADGPRSFH
IGLNVYAFTALMFVLGIAMAFGKASVFKYISDDYPANIGAISGIVGLAGGLGGFVLPILFGVLLDWTGVRSSAFMLMYGV
VWVSLAWMYFTEVRRTDVMDAGRGAADR
>Mature_427_residues
ADTASATPDGTPRRTRQAWSVLAVSTLAFTVCFMVWMMFGVIGIPIKKMLGLNATQFGLLTAMPVLTGSLVRVPLGIWTD
RYGGRIVMACLMAATVPAIWLMGYATQYWHFLAIGLFVGLAGGSFSVGTPYVARWFPKERQGTAMGIYGAGNSGAAVNKF
VAPAILVAFGWAAVPHAYAAIMLGTLVLFWLFSHSDPSHRVPSHVTFMDQLQALKDPRVLKYCQYYSIVFGGYVALSLWM
VQYYVGEYSLDIRVAALLAACFSLPGGVLRAIGGALSDKYGAHSVTWWVLWVCWVCLFLLSYPQTDFTVLAADGPRSFHI
GLNVYAFTALMFVLGIAMAFGKASVFKYISDDYPANIGAISGIVGLAGGLGGFVLPILFGVLLDWTGVRSSAFMLMYGVV
WVSLAWMYFTEVRRTDVMDAGRGAADR

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=448, Percent_Identity=27.2321428571429, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787475, Length=449, Percent_Identity=27.3942093541203, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46478; Mature: 46347

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCC
>Mature Secondary Structure 
ADTASATPDGTPRRTRQAWSVLAVSTLAFTVCFMVWMMFGVIGIPIKKMLGLNATQFGL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHH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CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]