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The map label for this gene is narK [H]

Identifier: 120609359

GI number: 120609359

Start: 714905

End: 716368

Strand: Direct

Name: narK [H]

Synonym: Aave_0660

Alternate gene names: 120609359

Gene position: 714905-716368 (Clockwise)

Preceding gene: 120609358

Following gene: 120609360

Centisome position: 13.36

GC content: 69.54

Gene sequence:

>1464_bases
ATGCCTGCCACCCCTTCTTCCTCTTCTTCCTCTCCCTCCCGCGGCGGTGCCCGGGCCGGGCGCGTGCTGACCCTGTGGAC
CCCGGAGGACAAGGAGTTCTGGGAACGCGAAGGCGAAGCCATCGCGCGCCTGAACCTGTGGATCTCGGTGCCCGCGCTGT
TCCTGTCCTTCGCCATCTGGCAGGTCTGGAGCGTGGTCGCCGTGAGCCTGCCGGCGCTGGGCTTCCGCTATTCCACCAAC
CAGCTCTTCTGGCTGGCGGCGGCGCCCGCGCTCTCGGGTGCCACGCTGCGCATCTTCTACTCGTTCATGGTGCCCCTGGT
GGGCGGGCGCCGCTGGACGGCGATCTCCACCGCGTCGCTGCTGGTCCCGGCCCTCGGCATCGGCTTCGCGGTGCAGGACA
GCACGACGGCCTACCCGACGATGCTGGCGCTCGCGCTGCTGTGCGGCCTGGGCGGCGGCAATTTCAGCTCCAGCATGGCC
AACATCAGCTTCTTCTTCCCGAAGGAGCGCAAGGGTTCGGCGCTGGGCGTGAACGCGGGGCTGGGCAACCTGGGCGTGTC
GGTGGTGCAGTTCCTGAGCCCGCTGGTGATCACGGCCGGCGTGTTCGGCGTGCTGGGCGGCGATGCGCAGACGATGGTGA
AGAACGGCCAGGCGCAGCAGGTGTGGGCGCAGAACGCGGCCTTCGTCTGGGTGCCCTGGATCGCGCTCGCGTCGCTGGCC
GCATGGTTCGGCATGAACGACATCGCCGACGCGCGCGCCTCGTTCGCGGCCCAGGCCGCCATCTTCCGCCGCAAGCACAA
CTGGATCATGTGCGTGCTGTACCTGGGCACCTTCGGCTCGTTCATCGGCTTTTCCGCGGGCTTTCCGCTGCTCATCAAGA
GCCAGTTCCCCGAGGTGAATCCGCTCGCCTACGCATGGCTCGGGCCGCTGGTGGGCGCGCTGATCCGGCCCGTGGGCGGA
TGGCTGTCGGACAAGCTGGGCGGCGCGCGGGTCACGCTGTGGAACTTCGTGGCGATGGCCCTGGCCGTGCTGGGCGTGCT
GGCCTTCCTGCCCAAGGGCACGGGTGGCTACGCGCTCGCCTTCGGGCCGGCGCAGGGCAGCTTCGCGGGCTTCTTCGCGA
TGTTCATGGTGCTGTTCCTGACGACCGGCATCGGCAACGGATCGACCTTCCGGATGATCCCCGTGATCTTCCTGAACCTG
CGCCTGGCCGAAGTACGCACGGGCGGCGCCGGGGCCCGCGCCCAGGCCACCCGCGAGGGCAACACCGAAGGCGCCGCGGC
CCTGGGCTTCGCGGGCGCGCTGGGCGCCTACGGCGGCTTCTTCATCCCCAAGAGCTACGGCAGCTCCATCGCCGCGACGG
GGGGGCCGGAACTGGCGCTGTGGGCGTTCGCCGCGTTCTACGCGCTCTGCATCGGCATCACCTGGTGGTACTACGCGCGC
CGCCATGCAGAGATGCCGTGTTGA

Upstream 100 bases:

>100_bases
CTGGCCTGGATGTATTTCACCGAGGTGCGCCGCACCGACGTGATGGACGCCGGCCGCGGCGCGGCGGACCGTTGAATCCC
CAATGCAAGGAGACCGTGAC

Downstream 100 bases:

>100_bases
TGACCTACCCCCTGTGCGGCTGCGCCGCTTCCCCCTTCTCTCACGCTGCGCGTGGGAAGGGGGACCACACCGGTGGCCCG
GCCAAGCCGGTTCCACGGTG

Product: nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPATPSSSSSSPSRGGARAGRVLTLWTPEDKEFWEREGEAIARLNLWISVPALFLSFAIWQVWSVVAVSLPALGFRYSTN
QLFWLAAAPALSGATLRIFYSFMVPLVGGRRWTAISTASLLVPALGIGFAVQDSTTAYPTMLALALLCGLGGGNFSSSMA
NISFFFPKERKGSALGVNAGLGNLGVSVVQFLSPLVITAGVFGVLGGDAQTMVKNGQAQQVWAQNAAFVWVPWIALASLA
AWFGMNDIADARASFAAQAAIFRRKHNWIMCVLYLGTFGSFIGFSAGFPLLIKSQFPEVNPLAYAWLGPLVGALIRPVGG
WLSDKLGGARVTLWNFVAMALAVLGVLAFLPKGTGGYALAFGPAQGSFAGFFAMFMVLFLTTGIGNGSTFRMIPVIFLNL
RLAEVRTGGAGARAQATREGNTEGAAALGFAGALGAYGGFFIPKSYGSSIAATGGPELALWAFAAFYALCIGITWWYYAR
RHAEMPC

Sequences:

>Translated_487_residues
MPATPSSSSSSPSRGGARAGRVLTLWTPEDKEFWEREGEAIARLNLWISVPALFLSFAIWQVWSVVAVSLPALGFRYSTN
QLFWLAAAPALSGATLRIFYSFMVPLVGGRRWTAISTASLLVPALGIGFAVQDSTTAYPTMLALALLCGLGGGNFSSSMA
NISFFFPKERKGSALGVNAGLGNLGVSVVQFLSPLVITAGVFGVLGGDAQTMVKNGQAQQVWAQNAAFVWVPWIALASLA
AWFGMNDIADARASFAAQAAIFRRKHNWIMCVLYLGTFGSFIGFSAGFPLLIKSQFPEVNPLAYAWLGPLVGALIRPVGG
WLSDKLGGARVTLWNFVAMALAVLGVLAFLPKGTGGYALAFGPAQGSFAGFFAMFMVLFLTTGIGNGSTFRMIPVIFLNL
RLAEVRTGGAGARAQATREGNTEGAAALGFAGALGAYGGFFIPKSYGSSIAATGGPELALWAFAAFYALCIGITWWYYAR
RHAEMPC
>Mature_486_residues
PATPSSSSSSPSRGGARAGRVLTLWTPEDKEFWEREGEAIARLNLWISVPALFLSFAIWQVWSVVAVSLPALGFRYSTNQ
LFWLAAAPALSGATLRIFYSFMVPLVGGRRWTAISTASLLVPALGIGFAVQDSTTAYPTMLALALLCGLGGGNFSSSMAN
ISFFFPKERKGSALGVNAGLGNLGVSVVQFLSPLVITAGVFGVLGGDAQTMVKNGQAQQVWAQNAAFVWVPWIALASLAA
WFGMNDIADARASFAAQAAIFRRKHNWIMCVLYLGTFGSFIGFSAGFPLLIKSQFPEVNPLAYAWLGPLVGALIRPVGGW
LSDKLGGARVTLWNFVAMALAVLGVLAFLPKGTGGYALAFGPAQGSFAGFFAMFMVLFLTTGIGNGSTFRMIPVIFLNLR
LAEVRTGGAGARAQATREGNTEGAAALGFAGALGAYGGFFIPKSYGSSIAATGGPELALWAFAAFYALCIGITWWYYARR
HAEMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=478, Percent_Identity=52.510460251046, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787743, Length=461, Percent_Identity=51.8438177874187, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51512; Mature: 51381

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATPSSSSSSPSRGGARAGRVLTLWTPEDKEFWEREGEAIARLNLWISVPALFLSFAIW
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
QVWSVVAVSLPALGFRYSTNQLFWLAAAPALSGATLRIFYSFMVPLVGGRRWTAISTASL
HHHHHHHHHHHHCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHH
LVPALGIGFAVQDSTTAYPTMLALALLCGLGGGNFSSSMANISFFFPKERKGSALGVNAG
HHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCC
LGNLGVSVVQFLSPLVITAGVFGVLGGDAQTMVKNGQAQQVWAQNAAFVWVPWIALASLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCEEEHHHHHHHHHH
AWFGMNDIADARASFAAQAAIFRRKHNWIMCVLYLGTFGSFIGFSAGFPLLIKSQFPEVN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCC
PLAYAWLGPLVGALIRPVGGWLSDKLGGARVTLWNFVAMALAVLGVLAFLPKGTGGYALA
CHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
FGPAQGSFAGFFAMFMVLFLTTGIGNGSTFRMIPVIFLNLRLAEVRTGGAGARAQATREG
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
NTEGAAALGFAGALGAYGGFFIPKSYGSSIAATGGPELALWAFAAFYALCIGITWWYYAR
CCCCCHHHHHHHHHHHCCCEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHAEMPC
HHCCCCC
>Mature Secondary Structure 
PATPSSSSSSPSRGGARAGRVLTLWTPEDKEFWEREGEAIARLNLWISVPALFLSFAIW
CCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
QVWSVVAVSLPALGFRYSTNQLFWLAAAPALSGATLRIFYSFMVPLVGGRRWTAISTASL
HHHHHHHHHHHHCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHH
LVPALGIGFAVQDSTTAYPTMLALALLCGLGGGNFSSSMANISFFFPKERKGSALGVNAG
HHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCC
LGNLGVSVVQFLSPLVITAGVFGVLGGDAQTMVKNGQAQQVWAQNAAFVWVPWIALASLA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCEEEHHHHHHHHHH
AWFGMNDIADARASFAAQAAIFRRKHNWIMCVLYLGTFGSFIGFSAGFPLLIKSQFPEVN
HHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCC
PLAYAWLGPLVGALIRPVGGWLSDKLGGARVTLWNFVAMALAVLGVLAFLPKGTGGYALA
CHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
FGPAQGSFAGFFAMFMVLFLTTGIGNGSTFRMIPVIFLNLRLAEVRTGGAGARAQATREG
ECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
NTEGAAALGFAGALGAYGGFFIPKSYGSSIAATGGPELALWAFAAFYALCIGITWWYYAR
CCCCCHHHHHHHHHHHCCCEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RHAEMPC
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]