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The map label for this gene is queA [H]

Identifier: 120609290

GI number: 120609290

Start: 644741

End: 645850

Strand: Reverse

Name: queA [H]

Synonym: Aave_0590

Alternate gene names: 120609290

Gene position: 645850-644741 (Counterclockwise)

Preceding gene: 120609291

Following gene: 120609282

Centisome position: 12.07

GC content: 72.34

Gene sequence:

>1110_bases
ATGTCCGCCAGCCAGCCACCCCGCGCCTTCACCCTCAGCGATTTCGACTTCTCGCTGCCCCCCGAACTCATCGCCCAGCA
CCCCGCGCCCGAGCGCAGCGCATCGCGCCTGCTCGATGGCCGTGCGGACGTGCCCGTGGACCGCATCTTTCGCGAGCTGC
CGGGCCTGCTGCAGCCGGGCGACCTGCTGGTGTTCAACGACACGCGCGTGGTCAAGGCCCGCGTGTTCGGCGAGAAGGCC
AGCGGCGGCAAGGTGGAGCTGCTGATCGAGCGCGTGCTCGTGGGCACTGGCGGCGAGGCGGGCAACGAGGTGGTCGCCCA
CATGAAGGTGAGCAAGAAGCCCGCCGTGGGCGCCATGCTCCACATGGCCGGGGGCCCGGCGGGCGGGGGCTTCGGCGCGA
CGCTGCTGGGCCGGTGGCCCGACGAGGACGGCCCGCTGTTCCGCTTCGCGCTGTCGAGCCCCGACGGCGCGGGCCCGCAT
GCGCTGATGGAGCGCCACGGCCACATACCGCTGCCGCCCTACATCGAGCGCCACCAGGAAGGCGGCGGCGATCCCGATGC
GGCCGAGGATGCGGAGCGCTACCAGACGGTGTTCGCGCGCGCGCCGGGTGCGGTGGCGGCGCCCACGGCGGCGCTGCATT
TCGACGAAGGGGTGCTCGCGTCGCTCGCGGCCCGCGGCGTGGAGCGCGCGAGCGTCACGCTGCACGTGGGCGCCGGCACC
TTCCAGCCCGTGAAGACCGAGAGCCTGGCGGACCACCGCATGCACAGCGAGTGGTACGAGGTGCCGCTGGCCACGCTGGC
CGCGCTGGAGCGCTGCCGGCAGCGCGGCGGCCGCGTGGTGGCCGTGGGCACGACGACGGTGCGCACGCTCGAATCCTGGG
CGCGCAGCGGGCAGGGCACGGGCGATACCGACATCTTCATCACGCCGGGCTTCGCCTTCCAGGTGGTGGACCTGCTGGTC
ACCAACTTCCACCTGCCCAAGAGCACGCTGATGATGCTGATCAGCGCCTTCACGGGCTACGAGCACGCCATGGACCTCTA
CCGCCACGCGATCGCGCAGCGCTACCGGTTCTTCAGCTACGGCGACGCGATGCTGCTGGCGCGGCGCTGA

Upstream 100 bases:

>100_bases
ACCGGGCCCAGCAAAGCGCTCACCCCTCCATGGGAGAATCGAAGGCTTCGCCTTCCTGATCCCTCTCCTTGGAACGGGCC
CGTCCGGCCCTCAAGCTCCC

Downstream 100 bases:

>100_bases
CGCGGCACCGCCCCCACGGGGGCCGCGGGCGGGGACGGGGCTTGTGGGCAACGCTCAGTACGCGACGGCGTGCCCGTCCT
TGCGCGGGTCCGAACCCGCG

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSASQPPRAFTLSDFDFSLPPELIAQHPAPERSASRLLDGRADVPVDRIFRELPGLLQPGDLLVFNDTRVVKARVFGEKA
SGGKVELLIERVLVGTGGEAGNEVVAHMKVSKKPAVGAMLHMAGGPAGGGFGATLLGRWPDEDGPLFRFALSSPDGAGPH
ALMERHGHIPLPPYIERHQEGGGDPDAAEDAERYQTVFARAPGAVAAPTAALHFDEGVLASLAARGVERASVTLHVGAGT
FQPVKTESLADHRMHSEWYEVPLATLAALERCRQRGGRVVAVGTTTVRTLESWARSGQGTGDTDIFITPGFAFQVVDLLV
TNFHLPKSTLMMLISAFTGYEHAMDLYRHAIAQRYRFFSYGDAMLLARR

Sequences:

>Translated_369_residues
MSASQPPRAFTLSDFDFSLPPELIAQHPAPERSASRLLDGRADVPVDRIFRELPGLLQPGDLLVFNDTRVVKARVFGEKA
SGGKVELLIERVLVGTGGEAGNEVVAHMKVSKKPAVGAMLHMAGGPAGGGFGATLLGRWPDEDGPLFRFALSSPDGAGPH
ALMERHGHIPLPPYIERHQEGGGDPDAAEDAERYQTVFARAPGAVAAPTAALHFDEGVLASLAARGVERASVTLHVGAGT
FQPVKTESLADHRMHSEWYEVPLATLAALERCRQRGGRVVAVGTTTVRTLESWARSGQGTGDTDIFITPGFAFQVVDLLV
TNFHLPKSTLMMLISAFTGYEHAMDLYRHAIAQRYRFFSYGDAMLLARR
>Mature_368_residues
SASQPPRAFTLSDFDFSLPPELIAQHPAPERSASRLLDGRADVPVDRIFRELPGLLQPGDLLVFNDTRVVKARVFGEKAS
GGKVELLIERVLVGTGGEAGNEVVAHMKVSKKPAVGAMLHMAGGPAGGGFGATLLGRWPDEDGPLFRFALSSPDGAGPHA
LMERHGHIPLPPYIERHQEGGGDPDAAEDAERYQTVFARAPGAVAAPTAALHFDEGVLASLAARGVERASVTLHVGAGTF
QPVKTESLADHRMHSEWYEVPLATLAALERCRQRGGRVVAVGTTTVRTLESWARSGQGTGDTDIFITPGFAFQVVDLLVT
NFHLPKSTLMMLISAFTGYEHAMDLYRHAIAQRYRFFSYGDAMLLARR

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=363, Percent_Identity=49.862258953168, Blast_Score=332, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39752; Mature: 39621

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASQPPRAFTLSDFDFSLPPELIAQHPAPERSASRLLDGRADVPVDRIFRELPGLLQPG
CCCCCCCCEEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCC
DLLVFNDTRVVKARVFGEKASGGKVELLIERVLVGTGGEAGNEVVAHMKVSKKPAVGAML
CEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHE
HMAGGPAGGGFGATLLGRWPDEDGPLFRFALSSPDGAGPHALMERHGHIPLPPYIERHQE
EECCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHC
GGGDPDAAEDAERYQTVFARAPGAVAAPTAALHFDEGVLASLAARGVERASVTLHVGAGT
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEEEEEECCCC
FQPVKTESLADHRMHSEWYEVPLATLAALERCRQRGGRVVAVGTTTVRTLESWARSGQGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCC
GDTDIFITPGFAFQVVDLLVTNFHLPKSTLMMLISAFTGYEHAMDLYRHAIAQRYRFFSY
CCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GDAMLLARR
CCEEEEECC
>Mature Secondary Structure 
SASQPPRAFTLSDFDFSLPPELIAQHPAPERSASRLLDGRADVPVDRIFRELPGLLQPG
CCCCCCCEEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCC
DLLVFNDTRVVKARVFGEKASGGKVELLIERVLVGTGGEAGNEVVAHMKVSKKPAVGAML
CEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHE
HMAGGPAGGGFGATLLGRWPDEDGPLFRFALSSPDGAGPHALMERHGHIPLPPYIERHQE
EECCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHC
GGGDPDAAEDAERYQTVFARAPGAVAAPTAALHFDEGVLASLAARGVERASVTLHVGAGT
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHCCCCEEEEEEEECCCC
FQPVKTESLADHRMHSEWYEVPLATLAALERCRQRGGRVVAVGTTTVRTLESWARSGQGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCC
GDTDIFITPGFAFQVVDLLVTNFHLPKSTLMMLISAFTGYEHAMDLYRHAIAQRYRFFSY
CCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
GDAMLLARR
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA