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The map label for this gene is asnB

Identifier: 120599234

GI number: 120599234

Start: 2804071

End: 2805735

Strand: Direct

Name: asnB

Synonym: Sputw3181_2430

Alternate gene names: 120599234

Gene position: 2804071-2805735 (Clockwise)

Preceding gene: 120599233

Following gene: 120599238

Centisome position: 59.55

GC content: 47.45

Gene sequence:

>1665_bases
ATGTGTTCAATCTTTTCGATTTTAGATATCCAATCTGATGCCAAACAACTGCGCCAAGTCGCGCTAGAAATGTCTAAGCT
TATGCGCCATCGTGGCCCTGACTGGTCAGGTATTTATGCCAGCGATAAAGCGATTCTTGCCCATGAGCGTTTAGCGATTG
TTGACATTGAACACGGTGCCCAGCCGCTGCTGACTGAAGATGGCAGTTTGATCCTCGCGGTCAACGGCGAAATCTATAAC
CACAAAGAATTGAAAGCCCAGTTAGGCGATAAGTACAGCTATCAAACCAACTCTGACTGCGAAGTGATCTTGGCGCTGTA
TCAAGAATACGGCACTGAATTTCTAGATAAATTAAACGGTATCTTCGCCTTTGTGTTATATGACAAAGCTCAAGATAGTT
ACCTTATCGGCCGCGATCACATGGGTATCATCCCACTATACACAGGCCGTGACGCTGCGGGTAACTTCTATGTTGCCTCA
GAGATGAAAGCCTTGATGCCAGTGTGTAAAACCGTAGAAGAATTCCAACCTGGCCAATATTTTTATTCAAAGGATGGTGA
AGCCGTTAAATACTATAGCCGAGATTGGCAAAGCTATGATGCAGTTAAAGATAATCCAGCCAGCCAAGAAGAATTGCGTG
ACGCATTAGAAGCGGCGGTCAAACGTCAATTAATGTCAGATGTGCCCTATGGTGTGTTGCTGTCAGGCGGCTTAGATTCA
TCGATCATTTCCGCCATCACCCAAACCTTTGCTAAGCGCAGAATTGAAGATGATGGTGAGACCAACGCTTGGTGGCCACA
GTTGCATTCCTTTGCCGTCGGTCTAAAAGGTGCGCCCGATCTTATCGCCGCCAAAAAAGTCGCCGATGCCATTGGTACGA
TTCACCATGAGATCCATTTCACCTTCCAAGAAGGCTTAGATGCGATTAAAGAAGTGATCTATCACTTAGAAACCTACGAT
GTCACCACCATACGTGCTGCCACGCCAATGTATTTAATGGCGCGTAAAATCAAAGCTATGGGCATTAAAATGGTGTTATC
GGGCGAAGGTGCTGACGAGTTATTTGGCGGCTATTTATACTTCCATAAAGCGCCAAACGCACAGGCATTCCACGAGGAAT
TAGTGCGCAAACTCGATAAACTGCATCTGTTCGATTGTCTGCGTGCCAACAAAGCCATGGCCGCTTGGGGATTAGAAGCT
CGCGTGCCCTTCCTCGATAAAGAGTTTATGGATGTGGCAATGCGTATCAACCCTGAAGCGAAAATGTCTAAGGATGGCCG
TATTGAGAAACACATTCTGCGTAAAGCCTTTGAGCATAAATTGCCAAAAGAAGTGGCGTGGCGTCAGAAGGAACAATTCA
GTGACGGCGTGGGCTACTCTTGGATTGACGGTTTGAAAGAACAAGCGGCGGCGCAGGTCGATGATTTGCAACTGGCGAAT
GCGAAATTCCGCTTCCCCTATAACACGCCAGAAACCAAAGAGGCGTACTTCTATCGCTGCTTCTTTGAGGAACATTATCC
ACTGAGCGGCGCTGCTGAAACGGTTCCAGGCGGTAAATCCGTCGCCTGTTCAACACCAGAAGCACTAGCTTGGGACGAAA
GCTTACGCGGCATTATCGACCCATCGGGCCGAGCAGTACGCTCAGTGCATACCGCAAGTTATTAA

Upstream 100 bases:

>100_bases
ATAAATCATCAAAAATAGTGTTTGGCAAAACGCTAAATAGCTGGCACATTAAACCTCAATTGAGACATCCTCAAATGACA
GACAGTGTGGGAGTTGTACT

Downstream 100 bases:

>100_bases
TGGTCTAACATTGATGGACTCGATTCATAAACTAGGTTGACTAACTGATTCAGACCCCCAACCTAAGTGCACTTAGTGCG
ACAGACTTACCGAACAAATA

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFSILDIQSDAKQLRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKAQDSYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFQPGQYFYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAITQTFAKRRIEDDGETNAWWPQLHSFAVGLKGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTASY

Sequences:

>Translated_554_residues
MCSIFSILDIQSDAKQLRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKAQDSYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFQPGQYFYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAITQTFAKRRIEDDGETNAWWPQLHSFAVGLKGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTASY
>Mature_554_residues
MCSIFSILDIQSDAKQLRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNGIFAFVLYDKAQDSYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFQPGQYFYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAITQTFAKRRIEDDGETNAWWPQLHSFAVGLKGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTASY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010848, Length=514, Percent_Identity=42.6070038910506, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=41.6206261510129, Blast_Score=348, Evalue=8e-96,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=41.6206261510129, Blast_Score=348, Evalue=8e-96,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=41.6206261510129, Blast_Score=348, Evalue=8e-96,
Organism=Homo sapiens, GI296010852, Length=449, Percent_Identity=43.652561247216, Blast_Score=317, Evalue=3e-86,
Organism=Homo sapiens, GI296010850, Length=449, Percent_Identity=43.652561247216, Blast_Score=317, Evalue=3e-86,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=70.3971119133574, Blast_Score=833, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=561, Percent_Identity=42.7807486631016, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71993933, Length=531, Percent_Identity=43.879472693032, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=35.5640535372849, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25147560, Length=312, Percent_Identity=39.4230769230769, Blast_Score=197, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=50.4587155963303, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=51.0989010989011, Blast_Score=538, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45553209, Length=581, Percent_Identity=46.9879518072289, Blast_Score=502, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62330; Mature: 62330

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFSILDIQSDAKQLRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IFAFVLYDKAQDSYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVEEFQPGQY
EEEEEEEECCCCCEEECCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCE
FYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEEHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIISAITQTFAKRRIEDDGETNAWWPQLHSFAVGLKGAPDLIAAKKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHTASY
CCCHHHHHHHHCCC
>Mature Secondary Structure
MCSIFSILDIQSDAKQLRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTEFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
IFAFVLYDKAQDSYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVEEFQPGQY
EEEEEEEECCCCCEEECCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCE
FYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEEHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIISAITQTFAKRRIEDDGETNAWWPQLHSFAVGLKGAPDLIAAKKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAAQVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHTASY
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]