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The map label for this gene is eptA [H]

Identifier: 120598730

GI number: 120598730

Start: 2208418

End: 2210043

Strand: Direct

Name: eptA [H]

Synonym: Sputw3181_1915

Alternate gene names: 120598730

Gene position: 2208418-2210043 (Clockwise)

Preceding gene: 120598729

Following gene: 120598731

Centisome position: 46.9

GC content: 40.59

Gene sequence:

>1626_bases
GTGCTAACAAGATTTAAGACCTTATCAGTGAATCAATTTACGCTGATCACTGCATTGTTCTATGTGTGTATTTTTAATAT
CCCACTCTTTACTTTTATTCACGAAGGTATTGAAAAACAGCCTGAGGTAGATCCGCTTTTTATCGCCAGTATGCCACTGT
TTTTTACCTTTGCCCTTGGCTTGGTATTTTCGTTTTTTTCGGTGAAGTATCTACTAAAACCCTTGTTTATCGTGCTCACC
TTAATGTCGTCGACGGTATTTTTTGCCACGATGAAATACCAGGTTGTATTTGATTACGGCATGATTGAAAACATTTTTCA
GACGAATCAGGCGGAAGCCTGGATGTATATTAATGTTGCTTCAGTGGCTAATTTACTGTTAACAGGGATATTGCCAGCAT
ACCTTATCTATAAAGCCGATATTCAATACAAACCGTTCTTTAAGGAGTTGGCACATAAAGTATTTTTTATGCTAATAATG
CTTGCTGGTATCGGGGTGATTGGTTTTTTCTATTTTCAGGATTACGCCACGTTTGGTCGAAATAACGATGGCCTGAGACG
TTATATTGTGCCAACTTATTTTGTGGGTTCGGTCTCTAAGTACATGTATTCACATTATTTACAAACACCAATCCAATATG
AAACACTTGGATTGGATGCAAAAGATGTCAGTGCTACCCCTAACCATAAACCCAACTTATTGGTGCTGGTGGTCGGGGAA
ACTGCACGTTCGATGAACTACCAATATTATGGCTATAAAAAGCCAACCAATGCCTATACCCAAGATCTTGGCTTGATAGT
GTTTAAGGATACCCATTCCTGTGGTACCGCAACGGCGGTGTCCTTGCCCTGTATGTTTTCACGAATGGGGCGCAAAGATT
ACGATTCACGCCGCGCTTATGCTCAAGATACGGTTATGGATGTGCTGAAACATGCTGATATTGAGGTGAAGTGGTTTGAT
AATGACTCTGGGTGTAAAGGCGTTTGCGATCGCATAGAGAATATCACTGTTGATCTCAATAGTGATCTTAAACTCTGCTC
TGGGCAATATTGTTTTGATCAGGTGTTATTAGAGAAATTAGATGAGGCGCTTAATAGCGCAAAAGCGAAAGACACTGTGC
TTGCCTTGCATATTATTGGCAGTCACGGTCCAACTTATTTTCTACGTTATCCCCCGCAATACCGTCGATTTATACCCGAT
TGCCAACGCAGTGATATCCAAAATTGCACCAACGAAGAGTTGGTAAATACCTATGACAACACGCTGCTGTATACGGACTA
TATAGTGAGCGAAGTCGTTAAAAAACTTCAAAAACAGCAAGATAAATTTAATACCGCGATGTTATATCTCAGTGATCATG
GTGAATCATTGGGGGAAAAGGGGATGTATTTACATGGTGCGCCTTACAGTATTGCGCCGATAGAACAAACCAGTATCCCC
ATGTTAGCTTGGGTCTCTGATCATTTCAGTCAAGATAATCATTTAAATATGGCGTGCTTGGCCGAACATGCTGACAAGGG
AGGATACTCCCATGACAATTTATTCGATAGCCTCTTAGGGTTAATGAATGTGAAGACTGAGGTGTACCAAAGCCATCTTG
ATATTTTTGCCTCTTGTCGTAGCTAA

Upstream 100 bases:

>100_bases
GGGCGTCGCTTGCTACTATGGCGCGAAAATTAAGTCGTAAGATTACCTTAAGAAAATTAAGTTAGCTTCGGCCAACGGCC
AATTTGAGAGAGCGTTATCG

Downstream 100 bases:

>100_bases
TGGACGGTATTAGATAAGATAAACCTATGCGAATGTCAGGTATAGGTTTAAAACCATCACAATAATTATGCTTCCCCATA
TCCAAATTCGATCAGTTGAG

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLTRFKTLSVNQFTLITALFYVCIFNIPLFTFIHEGIEKQPEVDPLFIASMPLFFTFALGLVFSFFSVKYLLKPLFIVLT
LMSSTVFFATMKYQVVFDYGMIENIFQTNQAEAWMYINVASVANLLLTGILPAYLIYKADIQYKPFFKELAHKVFFMLIM
LAGIGVIGFFYFQDYATFGRNNDGLRRYIVPTYFVGSVSKYMYSHYLQTPIQYETLGLDAKDVSATPNHKPNLLVLVVGE
TARSMNYQYYGYKKPTNAYTQDLGLIVFKDTHSCGTATAVSLPCMFSRMGRKDYDSRRAYAQDTVMDVLKHADIEVKWFD
NDSGCKGVCDRIENITVDLNSDLKLCSGQYCFDQVLLEKLDEALNSAKAKDTVLALHIIGSHGPTYFLRYPPQYRRFIPD
CQRSDIQNCTNEELVNTYDNTLLYTDYIVSEVVKKLQKQQDKFNTAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
MLAWVSDHFSQDNHLNMACLAEHADKGGYSHDNLFDSLLGLMNVKTEVYQSHLDIFASCRS

Sequences:

>Translated_541_residues
MLTRFKTLSVNQFTLITALFYVCIFNIPLFTFIHEGIEKQPEVDPLFIASMPLFFTFALGLVFSFFSVKYLLKPLFIVLT
LMSSTVFFATMKYQVVFDYGMIENIFQTNQAEAWMYINVASVANLLLTGILPAYLIYKADIQYKPFFKELAHKVFFMLIM
LAGIGVIGFFYFQDYATFGRNNDGLRRYIVPTYFVGSVSKYMYSHYLQTPIQYETLGLDAKDVSATPNHKPNLLVLVVGE
TARSMNYQYYGYKKPTNAYTQDLGLIVFKDTHSCGTATAVSLPCMFSRMGRKDYDSRRAYAQDTVMDVLKHADIEVKWFD
NDSGCKGVCDRIENITVDLNSDLKLCSGQYCFDQVLLEKLDEALNSAKAKDTVLALHIIGSHGPTYFLRYPPQYRRFIPD
CQRSDIQNCTNEELVNTYDNTLLYTDYIVSEVVKKLQKQQDKFNTAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
MLAWVSDHFSQDNHLNMACLAEHADKGGYSHDNLFDSLLGLMNVKTEVYQSHLDIFASCRS
>Mature_541_residues
MLTRFKTLSVNQFTLITALFYVCIFNIPLFTFIHEGIEKQPEVDPLFIASMPLFFTFALGLVFSFFSVKYLLKPLFIVLT
LMSSTVFFATMKYQVVFDYGMIENIFQTNQAEAWMYINVASVANLLLTGILPAYLIYKADIQYKPFFKELAHKVFFMLIM
LAGIGVIGFFYFQDYATFGRNNDGLRRYIVPTYFVGSVSKYMYSHYLQTPIQYETLGLDAKDVSATPNHKPNLLVLVVGE
TARSMNYQYYGYKKPTNAYTQDLGLIVFKDTHSCGTATAVSLPCMFSRMGRKDYDSRRAYAQDTVMDVLKHADIEVKWFD
NDSGCKGVCDRIENITVDLNSDLKLCSGQYCFDQVLLEKLDEALNSAKAKDTVLALHIIGSHGPTYFLRYPPQYRRFIPD
CQRSDIQNCTNEELVNTYDNTLLYTDYIVSEVVKKLQKQQDKFNTAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
MLAWVSDHFSQDNHLNMACLAEHADKGGYSHDNLFDSLLGLMNVKTEVYQSHLDIFASCRS

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=529, Percent_Identity=40.0756143667297, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI87082286, Length=457, Percent_Identity=26.0393873085339, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082223, Length=258, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790392, Length=322, Percent_Identity=25.1552795031056, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61777; Mature: 61777

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRFKTLSVNQFTLITALFYVCIFNIPLFTFIHEGIEKQPEVDPLFIASMPLFFTFALG
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHH
LVFSFFSVKYLLKPLFIVLTLMSSTVFFATMKYQVVFDYGMIENIFQTNQAEAWMYINVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHCCCCCEEEEEEHH
SVANLLLTGILPAYLIYKADIQYKPFFKELAHKVFFMLIMLAGIGVIGFFYFQDYATFGR
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNDGLRRYIVPTYFVGSVSKYMYSHYLQTPIQYETLGLDAKDVSATPNHKPNLLVLVVGE
CCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECC
TARSMNYQYYGYKKPTNAYTQDLGLIVFKDTHSCGTATAVSLPCMFSRMGRKDYDSRRAY
CHHHCCCEEECCCCCCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
AQDTVMDVLKHADIEVKWFDNDSGCKGVCDRIENITVDLNSDLKLCSGQYCFDQVLLEKL
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCEEEEECCCCHHHCCCHHHHHHHHHHH
DEALNSAKAKDTVLALHIIGSHGPTYFLRYPPQYRRFIPDCQRSDIQNCTNEELVNTYDN
HHHHHCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHCCCCHHHHHHCCCCHHHHHHHCC
TLLYTDYIVSEVVKKLQKQQDKFNTAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
CEEEHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEECCCCEECCCCCCCCC
MLAWVSDHFSQDNHLNMACLAEHADKGGYSHDNLFDSLLGLMNVKTEVYQSHLDIFASCR
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure
MLTRFKTLSVNQFTLITALFYVCIFNIPLFTFIHEGIEKQPEVDPLFIASMPLFFTFALG
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHH
LVFSFFSVKYLLKPLFIVLTLMSSTVFFATMKYQVVFDYGMIENIFQTNQAEAWMYINVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHCCCCCEEEEEEHH
SVANLLLTGILPAYLIYKADIQYKPFFKELAHKVFFMLIMLAGIGVIGFFYFQDYATFGR
HHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NNDGLRRYIVPTYFVGSVSKYMYSHYLQTPIQYETLGLDAKDVSATPNHKPNLLVLVVGE
CCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECC
TARSMNYQYYGYKKPTNAYTQDLGLIVFKDTHSCGTATAVSLPCMFSRMGRKDYDSRRAY
CHHHCCCEEECCCCCCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
AQDTVMDVLKHADIEVKWFDNDSGCKGVCDRIENITVDLNSDLKLCSGQYCFDQVLLEKL
HHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCEEEEECCCCHHHCCCHHHHHHHHHHH
DEALNSAKAKDTVLALHIIGSHGPTYFLRYPPQYRRFIPDCQRSDIQNCTNEELVNTYDN
HHHHHCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHCCCCHHHHHHCCCCHHHHHHHCC
TLLYTDYIVSEVVKKLQKQQDKFNTAMLYLSDHGESLGEKGMYLHGAPYSIAPIEQTSIP
CEEEHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEECCCCEECCCCCCCCC
MLAWVSDHFSQDNHLNMACLAEHADKGGYSHDNLFDSLLGLMNVKTEVYQSHLDIFASCR
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]