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The map label for this gene is pleD [H]

Identifier: 120598731

GI number: 120598731

Start: 2210111

End: 2211217

Strand: Direct

Name: pleD [H]

Synonym: Sputw3181_1916

Alternate gene names: 120598731

Gene position: 2210111-2211217 (Clockwise)

Preceding gene: 120598730

Following gene: 120598740

Centisome position: 46.94

GC content: 39.75

Gene sequence:

>1107_bases
ATGCTTCCCCATATCCAAATTCGATCAGTTGAGCTAACTTATGTTATACAAAAAATTAACAATAATATGGTTTCAATATT
TATGTTTTCTGTTGAAAATAAAGAACTTGATTCCGCGGCGCAAATTTTGCGGCTAGCCGTGCCACAAATGTCGGCCCTTG
ATATCCCCGTTACCCCGGAAAACTATACGGTTTGGTATGAATATTTTGCGGGGACTAATCTTGACTTAAACCGTGCGATT
GATGGTTTTATCGCAAACGGTGTTGTGTTTACAAAAGAGGTTAATACAAGCTTATATAAAAATTTTATCCAAGAACAATC
CCCCGAGGTGATTGAGAATGTACAAATAGAGACTCAGATTTTAATCAATTCATTACTGTGCAAAATTGTACAATTGAACC
AAGGGACCGCGGATTTTTCGGGCACTTTAGCGGAATTTGGCATCAAGCTTCAATCCAATTCTGACATTAATACCTTAAAT
CAACTTGTTGATGGTGTGATGGATGAAATGCAAAATTTACTGATCAACAACCAAAACATGGAACAAAGCCTAAATGCCAT
GGGGCAAGAGGTACAACATCTTAAGTCGGAAATGGAAAATTTAAGTCTGGCGGCCATGACGGATCAATTAACGTCTTTAT
ATAATCGCCGCGCCTATGAAATTGCCATTCAAGACCATATTCACCGCGCCCAAGAACAGCATTCCCGTTGTAGCTTACTG
ATTGTTGATATAGATAGGTTTAAACAATTTAATGATACCTATGGTCATCAAATTGGCGATAAAGTATTAGCTTATGTCGC
ACTCGCCTTAAAGCAATGTGTTCGAGGTGATGACTTTGTTGCTCGCTATGGTGGCGAAGAGTTTGTAGTGCTTTTGCCAA
ATACCCATTTTCACGATGCTTTACAAGTTGCGGAAACCTTAAGAGAACGTATTTCAGAGCGGCACTTAACCATAGGTAAA
GATAAAAAATTATCCCTCGGCGCAATTACAGTATCGATCGGTCTTGCCTCATTACAAGAGGGTGATGATGCCGAAACGCT
CTTTAGCCGAGCGGATAAAGCCTTATATGAAGCTAAATCCGATGGGCGTAACTGTGTTAGGGGATAG

Upstream 100 bases:

>100_bases
CATCTTGATATTTTTGCCTCTTGTCGTAGCTAATGGACGGTATTAGATAAGATAAACCTATGCGAATGTCAGGTATAGGT
TTAAAACCATCACAATAATT

Downstream 100 bases:

>100_bases
AGATACCCAAACCTGCTCACAATACACAACGCCACATCTTCAGATTGTTTGAGTATCCAGTTCGCGAACTATTTTACTGG
GGAAATAAATCCCTTAGGTT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLPHIQIRSVELTYVIQKINNNMVSIFMFSVENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAI
DGFIANGVVFTKEVNTSLYKNFIQEQSPEVIENVQIETQILINSLLCKIVQLNQGTADFSGTLAEFGIKLQSNSDINTLN
QLVDGVMDEMQNLLINNQNMEQSLNAMGQEVQHLKSEMENLSLAAMTDQLTSLYNRRAYEIAIQDHIHRAQEQHSRCSLL
IVDIDRFKQFNDTYGHQIGDKVLAYVALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERHLTIGK
DKKLSLGAITVSIGLASLQEGDDAETLFSRADKALYEAKSDGRNCVRG

Sequences:

>Translated_368_residues
MLPHIQIRSVELTYVIQKINNNMVSIFMFSVENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAI
DGFIANGVVFTKEVNTSLYKNFIQEQSPEVIENVQIETQILINSLLCKIVQLNQGTADFSGTLAEFGIKLQSNSDINTLN
QLVDGVMDEMQNLLINNQNMEQSLNAMGQEVQHLKSEMENLSLAAMTDQLTSLYNRRAYEIAIQDHIHRAQEQHSRCSLL
IVDIDRFKQFNDTYGHQIGDKVLAYVALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERHLTIGK
DKKLSLGAITVSIGLASLQEGDDAETLFSRADKALYEAKSDGRNCVRG
>Mature_368_residues
MLPHIQIRSVELTYVIQKINNNMVSIFMFSVENKELDSAAQILRLAVPQMSALDIPVTPENYTVWYEYFAGTNLDLNRAI
DGFIANGVVFTKEVNTSLYKNFIQEQSPEVIENVQIETQILINSLLCKIVQLNQGTADFSGTLAEFGIKLQSNSDINTLN
QLVDGVMDEMQNLLINNQNMEQSLNAMGQEVQHLKSEMENLSLAAMTDQLTSLYNRRAYEIAIQDHIHRAQEQHSRCSLL
IVDIDRFKQFNDTYGHQIGDKVLAYVALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDALQVAETLRERISERHLTIGK
DKKLSLGAITVSIGLASLQEGDDAETLFSRADKALYEAKSDGRNCVRG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=44.1860465116279, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI145693134, Length=271, Percent_Identity=33.5793357933579, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=39.6449704142012, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787802, Length=180, Percent_Identity=38.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=185, Percent_Identity=37.2972972972973, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=32.5842696629214, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787541, Length=186, Percent_Identity=34.4086021505376, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=31.8435754189944, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=31.6770186335404, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=34.6820809248555, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=32.3170731707317, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787056, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=304, Percent_Identity=26.6447368421053, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788956, Length=176, Percent_Identity=29.5454545454545, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41422; Mature: 41422

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPHIQIRSVELTYVIQKINNNMVSIFMFSVENKELDSAAQILRLAVPQMSALDIPVTPE
CCCCEEEEEEHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
NYTVWYEYFAGTNLDLNRAIDGFIANGVVFTKEVNTSLYKNFIQEQSPEVIENVQIETQI
CCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCHHHHH
LINSLLCKIVQLNQGTADFSGTLAEFGIKLQSNSDINTLNQLVDGVMDEMQNLLINNQNM
HHHHHHHHHHHCCCCCCCCCCHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCH
EQSLNAMGQEVQHLKSEMENLSLAAMTDQLTSLYNRRAYEIAIQDHIHRAQEQHSRCSLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHCCCCEEE
IVDIDRFKQFNDTYGHQIGDKVLAYVALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHH
LQVAETLRERISERHLTIGKDKKLSLGAITVSIGLASLQEGDDAETLFSRADKALYEAKS
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHC
DGRNCVRG
CCCCCCCC
>Mature Secondary Structure
MLPHIQIRSVELTYVIQKINNNMVSIFMFSVENKELDSAAQILRLAVPQMSALDIPVTPE
CCCCEEEEEEHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
NYTVWYEYFAGTNLDLNRAIDGFIANGVVFTKEVNTSLYKNFIQEQSPEVIENVQIETQI
CCEEEEEEECCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCHHHHH
LINSLLCKIVQLNQGTADFSGTLAEFGIKLQSNSDINTLNQLVDGVMDEMQNLLINNQNM
HHHHHHHHHHHCCCCCCCCCCHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCH
EQSLNAMGQEVQHLKSEMENLSLAAMTDQLTSLYNRRAYEIAIQDHIHRAQEQHSRCSLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHCCCCEEE
IVDIDRFKQFNDTYGHQIGDKVLAYVALALKQCVRGDDFVARYGGEEFVVLLPNTHFHDA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHH
LQVAETLRERISERHLTIGKDKKLSLGAITVSIGLASLQEGDDAETLFSRADKALYEAKS
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHC
DGRNCVRG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA