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The map label for this gene is ptrB [C]

Identifier: 120598729

GI number: 120598729

Start: 2206036

End: 2208129

Strand: Direct

Name: ptrB [C]

Synonym: Sputw3181_1914

Alternate gene names: 120598729

Gene position: 2206036-2208129 (Clockwise)

Preceding gene: 120598728

Following gene: 120598730

Centisome position: 46.85

GC content: 44.75

Gene sequence:

>2094_bases
ATGAATAATAAATTACTGCCTTGGTGCATTGCAGGAGTGCTCGGTATGAGCGGTCAACTTCACGCAGAAGAAGATAAATA
CATTTGGTTAGAAGATGTTGAAGGCGCAAAGCCGATGGAATGGGTGAAAACCCAAAATGCTGCATCTGCCGCCGAAATTA
AAGCGTTTAAAGGTTTTGATACCTTAGTGGCGAACAGTCTTGCCATCCTGAACGATAAAGAACGCATTCCCTACGCGACC
CACATTGGCGACAAGTTATACAACTTTTGGAAGGATGACACCCATGTGCGCGGTATTTATCGCCGTACTACCATGGAAGA
ATATGCTAAACCCGATCCCAAATGGGAGACTGTGCTTGATATCGATGCCCTAGGCAAAGCTGAATCCGTCAATTGGGTAT
TTAAGGATATTAGCTGTCAGTATCCCGAAAATGAGCGCTGTTTTGTCTCACTTTCCCGCGGCGGTGCCGATGCGGTGGAA
GTGCGAGAATTTAATTTAAATACTAAATCCTTTGTTGCCACCAAAGATAAGCCATTCTTTTTGAAAGAAGCTAAATCAAG
TCTGAGCTGGATAGATAAAGACCACGCCTTTGTTGGTACTGATTTTGGTGATGGTCAAAGCATGACTGACTCAGGTTATC
CACGGGAGGTGAAACTCTGGCAACGGGGTACACCACTCGAGCAAGCGAAACCGATTTTCACAGGCGACAAAACCTCAGTC
GCAGTATCAGGTTGGGTACTCTTCGATGATAAAACGCCCCTTAGCTTAGTCACCGAAGCACATACTTTTTATACCGCAAC
CCAATATGTTTACCAAGATGGCAAACTTATTAAACTACCAGTGCCAGAAGATGCTGAAATTAAAGGCTATTTTAAAGGTA
AGTTATTTATTGAGTTAAAGAGCGATTTAACTAATAAGGACGGTAATTTTAAGCAAGGCGCGGTCGTGTATGCCGATGTG
GCGGATCTTATCGCCCAAAAAGCCAAATTCAGTTTATTTGTGAGCCCGACTCAGACCGCATCGATTGCGCAGCTCAGTTT
TAGTAAGAGTGCTATATTCGTGAATTGGCTCGATAACGTGAAAAGCAAATTAGTGCGGTATGAGCAAGACGAAAAGGGCG
CTTGGCAAAGCACCCAAGTTCCATTCGAAGCGAATGGCGCCTTGACGGTAATGGACGTTGAGCAAGACAGTGATGATTTT
TTCGTGAATTACACCAGTTTTCTTGAGCCTTCAAGCCTCTATACGGTTAACGCAAAAACCTTAAATCCGAAGAAAATTAA
AGGTATGCCGCAACAGTTTGCTGCGGATAAGTTTAAAACTGAGCAATACTTTGCGACGTCTAAAGATGGCACTAAAGTAC
CGTACTTTGTGGTAATGGCTAAGGATCTTAAGTTAGATGGCACTAATCCTACGCTACTTTATGGCTATGGTGGGTTTGAA
GTCTCATTGCGTCCATCCTATTCGGCCACCATTGGTAAGAATTGGTTAGAGCAGGGCGGCGTATATGTGTTATCTAACAT
TCGTGGCGGTGGCGAATATGGCCCAGCTTGGCATCAAGCGGCGTTAAAGCAAAATCGACATAAAGCCTATGAGGATTTCG
AGGCAATAGCGGAAGATTTGATTGCCCGTAAAATCACGTCAAGCAAACATTTAGGCATCCAAGGTGGCAGCAACGGTGGC
CTGTTAATGGGGGCAGCATTTACCCGCAGGCCGGATCTTTACAACGCTGTTGTATGCCAAGTGCCTCTGCTTGATATGTT
GAGATTCAACAAGTTGCTAGCGGGTGCCAGTTGGATGGGTGAATACGGTAATCCTGATATTCCTGAGGAATGGGCTTACA
TTAAAACTTACTCTCCGTACCATAATCTGCATAAAGACGTGCATTATCCCAAGGTCTTTTTCACCACATCGACCCGTGAT
GACAGGGTTCACCCAGGCCACGCTCGTAAGATGGTGGCTAAGATGAAAGACATGGGAATTGATGTCTTGTACTACGAAAA
TATTGAAGGTGGCCACGCTGGCGCTGCGGATAATAATCAAGCTGCGGAACTTAATGCGATGACGTTTGCCTACTTATTAC
AGCGTTTACGATAA

Upstream 100 bases:

>100_bases
ACCTTTTGTTACATTCTTGTTGGATTAATAGTTGAACTGGAGATTAATTAAGGTAATGTAAGGGCAGATCTCAAGATGGA
CTTTGTTTAAAAGGAATAGA

Downstream 100 bases:

>100_bases
TTGTCATACGAGAAACACATAGACGTAATAACTCAGTCGTAAAAGATTGGGTTATAAAAGACAGAGTTGTAAAAACAGAG
TAGTAAAAACCGAGCAAGTC

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MNNKLLPWCIAGVLGMSGQLHAEEDKYIWLEDVEGAKPMEWVKTQNAASAAEIKAFKGFDTLVANSLAILNDKERIPYAT
HIGDKLYNFWKDDTHVRGIYRRTTMEEYAKPDPKWETVLDIDALGKAESVNWVFKDISCQYPENERCFVSLSRGGADAVE
VREFNLNTKSFVATKDKPFFLKEAKSSLSWIDKDHAFVGTDFGDGQSMTDSGYPREVKLWQRGTPLEQAKPIFTGDKTSV
AVSGWVLFDDKTPLSLVTEAHTFYTATQYVYQDGKLIKLPVPEDAEIKGYFKGKLFIELKSDLTNKDGNFKQGAVVYADV
ADLIAQKAKFSLFVSPTQTASIAQLSFSKSAIFVNWLDNVKSKLVRYEQDEKGAWQSTQVPFEANGALTVMDVEQDSDDF
FVNYTSFLEPSSLYTVNAKTLNPKKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGTNPTLLYGYGGFE
VSLRPSYSATIGKNWLEQGGVYVLSNIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIARKITSSKHLGIQGGSNGG
LLMGAAFTRRPDLYNAVVCQVPLLDMLRFNKLLAGASWMGEYGNPDIPEEWAYIKTYSPYHNLHKDVHYPKVFFTTSTRD
DRVHPGHARKMVAKMKDMGIDVLYYENIEGGHAGAADNNQAAELNAMTFAYLLQRLR

Sequences:

>Translated_697_residues
MNNKLLPWCIAGVLGMSGQLHAEEDKYIWLEDVEGAKPMEWVKTQNAASAAEIKAFKGFDTLVANSLAILNDKERIPYAT
HIGDKLYNFWKDDTHVRGIYRRTTMEEYAKPDPKWETVLDIDALGKAESVNWVFKDISCQYPENERCFVSLSRGGADAVE
VREFNLNTKSFVATKDKPFFLKEAKSSLSWIDKDHAFVGTDFGDGQSMTDSGYPREVKLWQRGTPLEQAKPIFTGDKTSV
AVSGWVLFDDKTPLSLVTEAHTFYTATQYVYQDGKLIKLPVPEDAEIKGYFKGKLFIELKSDLTNKDGNFKQGAVVYADV
ADLIAQKAKFSLFVSPTQTASIAQLSFSKSAIFVNWLDNVKSKLVRYEQDEKGAWQSTQVPFEANGALTVMDVEQDSDDF
FVNYTSFLEPSSLYTVNAKTLNPKKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGTNPTLLYGYGGFE
VSLRPSYSATIGKNWLEQGGVYVLSNIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIARKITSSKHLGIQGGSNGG
LLMGAAFTRRPDLYNAVVCQVPLLDMLRFNKLLAGASWMGEYGNPDIPEEWAYIKTYSPYHNLHKDVHYPKVFFTTSTRD
DRVHPGHARKMVAKMKDMGIDVLYYENIEGGHAGAADNNQAAELNAMTFAYLLQRLR
>Mature_697_residues
MNNKLLPWCIAGVLGMSGQLHAEEDKYIWLEDVEGAKPMEWVKTQNAASAAEIKAFKGFDTLVANSLAILNDKERIPYAT
HIGDKLYNFWKDDTHVRGIYRRTTMEEYAKPDPKWETVLDIDALGKAESVNWVFKDISCQYPENERCFVSLSRGGADAVE
VREFNLNTKSFVATKDKPFFLKEAKSSLSWIDKDHAFVGTDFGDGQSMTDSGYPREVKLWQRGTPLEQAKPIFTGDKTSV
AVSGWVLFDDKTPLSLVTEAHTFYTATQYVYQDGKLIKLPVPEDAEIKGYFKGKLFIELKSDLTNKDGNFKQGAVVYADV
ADLIAQKAKFSLFVSPTQTASIAQLSFSKSAIFVNWLDNVKSKLVRYEQDEKGAWQSTQVPFEANGALTVMDVEQDSDDF
FVNYTSFLEPSSLYTVNAKTLNPKKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGTNPTLLYGYGGFE
VSLRPSYSATIGKNWLEQGGVYVLSNIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDLIARKITSSKHLGIQGGSNGG
LLMGAAFTRRPDLYNAVVCQVPLLDMLRFNKLLAGASWMGEYGNPDIPEEWAYIKTYSPYHNLHKDVHYPKVFFTTSTRD
DRVHPGHARKMVAKMKDMGIDVLYYENIEGGHAGAADNNQAAELNAMTFAYLLQRLR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=386, Percent_Identity=32.6424870466321, Blast_Score=223, Evalue=6e-58,
Organism=Homo sapiens, GI284172420, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI284172413, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI70778815, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI284172438, Length=205, Percent_Identity=30.2439024390244, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI284172431, Length=205, Percent_Identity=30.2439024390244, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI108860686, Length=209, Percent_Identity=30.1435406698565, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI108860692, Length=258, Percent_Identity=27.5193798449612, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788150, Length=355, Percent_Identity=27.887323943662, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24583414, Length=332, Percent_Identity=37.6506024096386, Blast_Score=215, Evalue=9e-56,
Organism=Drosophila melanogaster, GI221510989, Length=281, Percent_Identity=36.6548042704626, Blast_Score=191, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 78211; Mature: 78211

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKLLPWCIAGVLGMSGQLHAEEDKYIWLEDVEGAKPMEWVKTQNAASAAEIKAFKGFD
CCCCCCHHHHHHHHCCCCCEECCCCCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHCCHH
TLVANSLAILNDKERIPYATHIGDKLYNFWKDDTHVRGIYRRTTMEEYAKPDPKWETVLD
HHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHCCCCCCCEEEEE
IDALGKAESVNWVFKDISCQYPENERCFVSLSRGGADAVEVREFNLNTKSFVATKDKPFF
HHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCH
LKEAKSSLSWIDKDHAFVGTDFGDGQSMTDSGYPREVKLWQRGTPLEQAKPIFTGDKTSV
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCCCCCCEE
AVSGWVLFDDKTPLSLVTEAHTFYTATQYVYQDGKLIKLPVPEDAEIKGYFKGKLFIELK
EEEEEEEECCCCCHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCEEEEEEEEEEEEEE
SDLTNKDGNFKQGAVVYADVADLIAQKAKFSLFVSPTQTASIAQLSFSKSAIFVNWLDNV
CCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEECCCCCCEEEEEECCCCEEEEEHHHHH
KSKLVRYEQDEKGAWQSTQVPFEANGALTVMDVEQDSDDFFVNYTSFLEPSSLYTVNAKT
HHHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCEEEEEHHHCCCCCEEEEECCC
LNPKKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGTNPTLLYGYGGFE
CCHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEECCEE
VSLRPSYSATIGKNWLEQGGVYVLSNIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDL
EEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IARKITSSKHLGIQGGSNGGLLMGAAFTRRPDLYNAVVCQVPLLDMLRFNKLLAGASWMG
HHHHHCCCCCEEEECCCCCCEEEEEECCCCCCHHHEHEECCCHHHHHHHHHHHHCCHHCC
EYGNPDIPEEWAYIKTYSPYHNLHKDVHYPKVFFTTSTRDDRVHPGHARKMVAKMKDMGI
CCCCCCCCCCCEEEEECCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
DVLYYENIEGGHAGAADNNQAAELNAMTFAYLLQRLR
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNKLLPWCIAGVLGMSGQLHAEEDKYIWLEDVEGAKPMEWVKTQNAASAAEIKAFKGFD
CCCCCCHHHHHHHHCCCCCEECCCCCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHCCHH
TLVANSLAILNDKERIPYATHIGDKLYNFWKDDTHVRGIYRRTTMEEYAKPDPKWETVLD
HHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEHHHHHCCHHHHCCCCCCCEEEEE
IDALGKAESVNWVFKDISCQYPENERCFVSLSRGGADAVEVREFNLNTKSFVATKDKPFF
HHHCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCH
LKEAKSSLSWIDKDHAFVGTDFGDGQSMTDSGYPREVKLWQRGTPLEQAKPIFTGDKTSV
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCCCCCCEE
AVSGWVLFDDKTPLSLVTEAHTFYTATQYVYQDGKLIKLPVPEDAEIKGYFKGKLFIELK
EEEEEEEECCCCCHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCEEEEEEEEEEEEEE
SDLTNKDGNFKQGAVVYADVADLIAQKAKFSLFVSPTQTASIAQLSFSKSAIFVNWLDNV
CCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEECCCCCCEEEEEECCCCEEEEEHHHHH
KSKLVRYEQDEKGAWQSTQVPFEANGALTVMDVEQDSDDFFVNYTSFLEPSSLYTVNAKT
HHHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCEEEEEHHHCCCCCEEEEECCC
LNPKKIKGMPQQFAADKFKTEQYFATSKDGTKVPYFVVMAKDLKLDGTNPTLLYGYGGFE
CCHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEEECCEE
VSLRPSYSATIGKNWLEQGGVYVLSNIRGGGEYGPAWHQAALKQNRHKAYEDFEAIAEDL
EEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IARKITSSKHLGIQGGSNGGLLMGAAFTRRPDLYNAVVCQVPLLDMLRFNKLLAGASWMG
HHHHHCCCCCEEEECCCCCCEEEEEECCCCCCHHHEHEECCCHHHHHHHHHHHHCCHHCC
EYGNPDIPEEWAYIKTYSPYHNLHKDVHYPKVFFTTSTRDDRVHPGHARKMVAKMKDMGI
CCCCCCCCCCCEEEEECCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
DVLYYENIEGGHAGAADNNQAAELNAMTFAYLLQRLR
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]