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The map label for this gene is nadE

Identifier: 120598531

GI number: 120598531

Start: 1972705

End: 1973535

Strand: Direct

Name: nadE

Synonym: Sputw3181_1714

Alternate gene names: 120598531

Gene position: 1972705-1973535 (Clockwise)

Preceding gene: 120598530

Following gene: 120598534

Centisome position: 41.9

GC content: 45.25

Gene sequence:

>831_bases
GTGAAAGCGCAAATCTTAAGAGAAATGAAGGTGCTCAAGGCGATTGAGCCAGAGTTTGAAGTGCAACGTCGTGTCGCGTT
TATCAAAACCACACTGAAAGAAGCGCGCAGTAAGGCACTCGTATTGGGGATCAGTGGTGGCGTTGACTCATCAACGGCGG
GGCGTTTATGTCAGCTAGCCGTTGATAGCTTAAACAGTGAACAAGCCAATAGTGATTATCAGTTTATTGCGGTGCGCTTA
CCCTATCATATCCAAAAAGATGAACATGAAGCCCAGCTTGCTTGTCAGTTTATTCAACCTTCGAAGCTTGTCACTGTAAA
TGTGCATCAAGGTGTTGATGCTGTACATGGTGCTACATTAGCGGCTTTTGTCGAGGCTGGGTTGACGTTACCCGATGCGG
CAAAGGTAGATTTTGTCAAAGGGAATGTTAAGGCCCGTATGCGCATGATCGCCCAATATGAACTTGCTGGACTTGTGGGC
GGTTTAGTTGTTGGTACAGATCATAGTGCTGAAAATATCACGGGTTTTTACACTAAATGGGGTGACGGGGCCTGTGATTT
AGCCCCATTATTTGGCTTGAACAAACGCCAAGTTCGCCAACTTGCAGCTTACCTTGGGGCACCAGAATCCTTAGTGTATA
AAGCGCCGACGGCTGATCTTGAAGATAATCAACCACTGTTAGAAGATGAAGTTGCATTGGGCTTAACCTATGCCCAAATT
GATGACTTTCTTGAAGGCAAAGAGGTTGATAAATCAGTTGAGACTAAGTTGATTAATATCTACAAAGCCACCCAACATAA
GCGTCAGCCTATTGCGACTATCTATGACTGA

Upstream 100 bases:

>100_bases
GCAGCATGATTTTTTGCTACAGGCAGTTGCAAATAAATGTTACCGTTAGTTTTGTTCTATTTGACCCAAATTACAGCGAT
TATTTAAGGTGGAATTTTCG

Downstream 100 bases:

>100_bases
TTTCTCTTGCTCGTTATGATTGCGCTAAGCACCCAGATGGTATGATCTAGGGTGCTTTTTTATGACTGATTAGAAGGTTC
TCTTGTCAGCAATACCAATC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKAQILREMKVLKAIEPEFEVQRRVAFIKTTLKEARSKALVLGISGGVDSSTAGRLCQLAVDSLNSEQANSDYQFIAVRL
PYHIQKDEHEAQLACQFIQPSKLVTVNVHQGVDAVHGATLAAFVEAGLTLPDAAKVDFVKGNVKARMRMIAQYELAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAYLGAPESLVYKAPTADLEDNQPLLEDEVALGLTYAQI
DDFLEGKEVDKSVETKLINIYKATQHKRQPIATIYD

Sequences:

>Translated_276_residues
MKAQILREMKVLKAIEPEFEVQRRVAFIKTTLKEARSKALVLGISGGVDSSTAGRLCQLAVDSLNSEQANSDYQFIAVRL
PYHIQKDEHEAQLACQFIQPSKLVTVNVHQGVDAVHGATLAAFVEAGLTLPDAAKVDFVKGNVKARMRMIAQYELAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAYLGAPESLVYKAPTADLEDNQPLLEDEVALGLTYAQI
DDFLEGKEVDKSVETKLINIYKATQHKRQPIATIYD
>Mature_276_residues
MKAQILREMKVLKAIEPEFEVQRRVAFIKTTLKEARSKALVLGISGGVDSSTAGRLCQLAVDSLNSEQANSDYQFIAVRL
PYHIQKDEHEAQLACQFIQPSKLVTVNVHQGVDAVHGATLAAFVEAGLTLPDAAKVDFVKGNVKARMRMIAQYELAGLVG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQLAAYLGAPESLVYKAPTADLEDNQPLLEDEVALGLTYAQI
DDFLEGKEVDKSVETKLINIYKATQHKRQPIATIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=280, Percent_Identity=51.7857142857143, Blast_Score=272, Evalue=1e-74,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30215; Mature: 30215

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQILREMKVLKAIEPEFEVQRRVAFIKTTLKEARSKALVLGISGGVDSSTAGRLCQLA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
VDSLNSEQANSDYQFIAVRLPYHIQKDEHEAQLACQFIQPSKLVTVNVHQGVDAVHGATL
HHHCCCCCCCCCEEEEEEEECEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
AAFVEAGLTLPDAAKVDFVKGNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKW
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLAAYLGAPESLVYKAPTADLEDNQPLLEDEVALGLTYAQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHCCHHHHH
DDFLEGKEVDKSVETKLINIYKATQHKRQPIATIYD
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKAQILREMKVLKAIEPEFEVQRRVAFIKTTLKEARSKALVLGISGGVDSSTAGRLCQLA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
VDSLNSEQANSDYQFIAVRLPYHIQKDEHEAQLACQFIQPSKLVTVNVHQGVDAVHGATL
HHHCCCCCCCCCEEEEEEEECEEECCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHH
AAFVEAGLTLPDAAKVDFVKGNVKARMRMIAQYELAGLVGGLVVGTDHSAENITGFYTKW
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQLAAYLGAPESLVYKAPTADLEDNQPLLEDEVALGLTYAQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHCCHHHHH
DDFLEGKEVDKSVETKLINIYKATQHKRQPIATIYD
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA