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The map label for this gene is ligA [H]

Identifier: 120598462

GI number: 120598462

Start: 1885107

End: 1887164

Strand: Direct

Name: ligA [H]

Synonym: Sputw3181_1645

Alternate gene names: 120598462

Gene position: 1885107-1887164 (Clockwise)

Preceding gene: 120598461

Following gene: 120598463

Centisome position: 40.04

GC content: 48.4

Gene sequence:

>2058_bases
ATGCAAGACATTCAACTCGATAAACTCCTTTCTGCCGATATGTCACCTGAGAATGCACAGCAGGTGATGCAGGCCCTTAG
CCAATCGCTGAACGAACACAATATTCGTTACTATGTGGATGATGCGCCAACTATTACTGATTCTGAGTATGATAGGTTGA
TGCAATGCTTGATAAAGCTCGAAGCGCAGTTCCCTCAGTTTATCGTTGCCGACTCGCCAACCCAGCGAGTCGGTGGGCTG
GCGCTGGCCAAGTTTGATCAAATCACTCACCTTAAGCCTATGTTGAGCTTAGATAACGCTTTCGGTGAAGCGGACTTCAG
CGCTTTTCATAAGCGCGTTACCGATAAAGTCGGTGAGGTGAGTTTTTGTTGTGAACCTAAACTCGATGGTTTAGCTGTTA
GTATTTTGTATCGCCATGGTGTACTCGAGCGCGCAGCGACCCGTGGCGATGGAACAGTAGGTGAAGACATCACTGAAAAT
GTGAAGACCATTAAATCCATTCCACTTAAATTGCGTGGCAATGATTTTCCTGAAGTGGTTGAAGTTCGCGGTGAAGCTTT
TATGCCCAAGGCTGCTTTTGAGGCTCTCAATGAGCGTGCGCGCGCAAAGGATGAAAAGTTGTTTGTCAATCCACGTAATG
CGGCGGCGGGGAGTTTACGTCAACTCGACAGTAAAATTACGGCCTCTCGCTCACTTGCTTTTTATGCTTATGCATTGGGT
GTGGTTGAGCCCGATTCTTATTCGTTGGGCAAAACCCACTATGAACAGTTACAGCAGCTAAAATCTTGGGGTTTACCCGT
TAGCAACGAAATCAAAGTGTGCGAAGAGTTAGATCAAGTATTCGATTATTACAAAGATATTCTTACCCGCCGCAGCGATT
TACCCTTTGAAATCGATGGTGTGGTGATGAAAGTTAACGATATTGCCCAACAGCAACGCTTAGGTTTTGTGGCTAAATCC
CCCCGTTGGGCCATTGCCTATAAGTTTCCCGCTCAAGAGGAAATGACATTACTTGAAGGTGTCGATTTTCAAGTAGGGCG
TACCGGTGCCGTCACCCCTGTTGCACGCTTAAAACCAGTATTTGTAGGGGGGGTAACTGTTTCTAATGCCACACTACACA
ATGCCGATGAGATTGAGCGTTTAGGCATTAAAATCGGTGATACTGTGATTATTCGCCGTGCTGGCGATGTTATCCCACAA
ATTGTGGCGATAGTGCCCGAAAGACGTCCTGAAACGGCGACTGATATCGTCTTTCCGCACAACTGTCCTGTTTGCGGCAG
TTTAGTCGAGCGTCTTGAAGGAGAGGCGGTGGCTCGCTGCAGCGGCGGTTTGTTTTGTGAGGCGCAGCGTAAAGAAGCGA
TTAAGCATTTCGCATCCCGCAAGGCGTTAGATATCGATGGTATGGGCGACAAAGTCGTCGAGCAATTGATTGATAAAGAA
CTCGTCCAAAGTCCTGCAGATTTGTTTAAGCTTACCGCTTCAATGATGACTATGCTCGATCGCATGGGCATGAAGTCGGC
AACCAATTTAGCGGCAGCGATTGAAGCGGCCAAAACCACAACACTTGCACGTTTCTTATATGCCTTAGGCATTCGTGAAG
TGGGCGAGGCGACGGCGGCAAACCTTGCGGCGCACTTTGCCAGTTTAGATGCGCTGCGCGTTGCGACTGTTGAGCAGTTA
ACTGCAGTGGAAGATGTGGGCGTTGTTGTTGCTCAGCATGTAGCACATTTTTTTGCTCAGCCCCATAATCTTGAAGTGAT
TGATGCCTTGATTGCAGCAGGAGTCCACTGGCCTGCTATTGAAGCGCCGAGCGCGGATGCACAACCCCTTAAAGGTCAGA
CTTGGGTATTAACGGGCACCTTAAACCAACTTAATCGTAACGATGCTAAGGCGCAGTTGCAGACCCTTGGGGCGAAAGTG
GCTGGCAGTGTTTCCAAAAATACCGATTGTTTAGTGGCAGGTGAGGCGGCAGGTTCAAAACTGGCTAAAGCGCAGGAACT
GGGGGTAAAGGTGATTGATGAAGAGGCGCTATTAGCACTCTTCGCGGCTAATCGCTAA

Upstream 100 bases:

>100_bases
CACCGTATTCGCGCTAATGCGTAACATTAAATCACTCTGACATCATGAGGCCGCAATTGCGGCCTCATTTTTGTGTTCTA
AAGAGATCTGGATAAAAAAT

Downstream 100 bases:

>100_bases
CAGCCACTCTCCCGTAAAGAGCACTCAAGTGTTGAATACGAAAAGAGTCTTGCGGTGCAAGGCTCTTTTTTAATTCAGTG
TTAAGTATTTGGTATCGGAC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MQDIQLDKLLSADMSPENAQQVMQALSQSLNEHNIRYYVDDAPTITDSEYDRLMQCLIKLEAQFPQFIVADSPTQRVGGL
ALAKFDQITHLKPMLSLDNAFGEADFSAFHKRVTDKVGEVSFCCEPKLDGLAVSILYRHGVLERAATRGDGTVGEDITEN
VKTIKSIPLKLRGNDFPEVVEVRGEAFMPKAAFEALNERARAKDEKLFVNPRNAAAGSLRQLDSKITASRSLAFYAYALG
VVEPDSYSLGKTHYEQLQQLKSWGLPVSNEIKVCEELDQVFDYYKDILTRRSDLPFEIDGVVMKVNDIAQQQRLGFVAKS
PRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIERLGIKIGDTVIIRRAGDVIPQ
IVAIVPERRPETATDIVFPHNCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKE
LVQSPADLFKLTASMMTMLDRMGMKSATNLAAAIEAAKTTTLARFLYALGIREVGEATAANLAAHFASLDALRVATVEQL
TAVEDVGVVVAQHVAHFFAQPHNLEVIDALIAAGVHWPAIEAPSADAQPLKGQTWVLTGTLNQLNRNDAKAQLQTLGAKV
AGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIDEEALLALFAANR

Sequences:

>Translated_685_residues
MQDIQLDKLLSADMSPENAQQVMQALSQSLNEHNIRYYVDDAPTITDSEYDRLMQCLIKLEAQFPQFIVADSPTQRVGGL
ALAKFDQITHLKPMLSLDNAFGEADFSAFHKRVTDKVGEVSFCCEPKLDGLAVSILYRHGVLERAATRGDGTVGEDITEN
VKTIKSIPLKLRGNDFPEVVEVRGEAFMPKAAFEALNERARAKDEKLFVNPRNAAAGSLRQLDSKITASRSLAFYAYALG
VVEPDSYSLGKTHYEQLQQLKSWGLPVSNEIKVCEELDQVFDYYKDILTRRSDLPFEIDGVVMKVNDIAQQQRLGFVAKS
PRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIERLGIKIGDTVIIRRAGDVIPQ
IVAIVPERRPETATDIVFPHNCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKE
LVQSPADLFKLTASMMTMLDRMGMKSATNLAAAIEAAKTTTLARFLYALGIREVGEATAANLAAHFASLDALRVATVEQL
TAVEDVGVVVAQHVAHFFAQPHNLEVIDALIAAGVHWPAIEAPSADAQPLKGQTWVLTGTLNQLNRNDAKAQLQTLGAKV
AGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIDEEALLALFAANR
>Mature_685_residues
MQDIQLDKLLSADMSPENAQQVMQALSQSLNEHNIRYYVDDAPTITDSEYDRLMQCLIKLEAQFPQFIVADSPTQRVGGL
ALAKFDQITHLKPMLSLDNAFGEADFSAFHKRVTDKVGEVSFCCEPKLDGLAVSILYRHGVLERAATRGDGTVGEDITEN
VKTIKSIPLKLRGNDFPEVVEVRGEAFMPKAAFEALNERARAKDEKLFVNPRNAAAGSLRQLDSKITASRSLAFYAYALG
VVEPDSYSLGKTHYEQLQQLKSWGLPVSNEIKVCEELDQVFDYYKDILTRRSDLPFEIDGVVMKVNDIAQQQRLGFVAKS
PRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNADEIERLGIKIGDTVIIRRAGDVIPQ
IVAIVPERRPETATDIVFPHNCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKE
LVQSPADLFKLTASMMTMLDRMGMKSATNLAAAIEAAKTTTLARFLYALGIREVGEATAANLAAHFASLDALRVATVEQL
TAVEDVGVVVAQHVAHFFAQPHNLEVIDALIAAGVHWPAIEAPSADAQPLKGQTWVLTGTLNQLNRNDAKAQLQTLGAKV
AGSVSKNTDCLVAGEAAGSKLAKAQELGVKVIDEEALLALFAANR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=61.3670133729569, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=530, Percent_Identity=22.0754716981132, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74638; Mature: 74638

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIQLDKLLSADMSPENAQQVMQALSQSLNEHNIRYYVDDAPTITDSEYDRLMQCLIKL
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
EAQFPQFIVADSPTQRVGGLALAKFDQITHLKPMLSLDNAFGEADFSAFHKRVTDKVGEV
HHCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCE
SFCCEPKLDGLAVSILYRHGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGNDFPEVV
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEECCCCCHHHH
EVRGEAFMPKAAFEALNERARAKDEKLFVNPRNAAAGSLRQLDSKITASRSLAFYAYALG
HHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VVEPDSYSLGKTHYEQLQQLKSWGLPVSNEIKVCEELDQVFDYYKDILTRRSDLPFEIDG
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
VVMKVNDIAQQQRLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPV
EEEEHHHHHHHHHCCCEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHCCEE
FVGGVTVSNATLHNADEIERLGIKIGDTVIIRRAGDVIPQIVAIVPERRPETATDIVFPH
EEECEEECCCCCCCHHHHHHCCCEECCEEEEECHHHHHHHHHHHCCCCCCCCCCCEECCC
NCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKE
CCCHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVQSPADLFKLTASMMTMLDRMGMKSATNLAAAIEAAKTTTLARFLYALGIREVGEATAA
HHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAAHFASLDALRVATVEQLTAVEDVGVVVAQHVAHFFAQPHNLEVIDALIAAGVHWPAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
EAPSADAQPLKGQTWVLTGTLNQLNRNDAKAQLQTLGAKVAGSVSKNTDCLVAGEAAGSK
CCCCCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHH
LAKAQELGVKVIDEEALLALFAANR
HHHHHHCCCEEECHHHHHHHHHCCC
>Mature Secondary Structure
MQDIQLDKLLSADMSPENAQQVMQALSQSLNEHNIRYYVDDAPTITDSEYDRLMQCLIKL
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
EAQFPQFIVADSPTQRVGGLALAKFDQITHLKPMLSLDNAFGEADFSAFHKRVTDKVGEV
HHCCCCEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCE
SFCCEPKLDGLAVSILYRHGVLERAATRGDGTVGEDITENVKTIKSIPLKLRGNDFPEVV
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEECCCCCHHHH
EVRGEAFMPKAAFEALNERARAKDEKLFVNPRNAAAGSLRQLDSKITASRSLAFYAYALG
HHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VVEPDSYSLGKTHYEQLQQLKSWGLPVSNEIKVCEELDQVFDYYKDILTRRSDLPFEIDG
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECC
VVMKVNDIAQQQRLGFVAKSPRWAIAYKFPAQEEMTLLEGVDFQVGRTGAVTPVARLKPV
EEEEHHHHHHHHHCCCEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHCCEE
FVGGVTVSNATLHNADEIERLGIKIGDTVIIRRAGDVIPQIVAIVPERRPETATDIVFPH
EEECEEECCCCCCCHHHHHHCCCEECCEEEEECHHHHHHHHHHHCCCCCCCCCCCEECCC
NCPVCGSLVERLEGEAVARCSGGLFCEAQRKEAIKHFASRKALDIDGMGDKVVEQLIDKE
CCCHHHHHHHHHCCCCEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVQSPADLFKLTASMMTMLDRMGMKSATNLAAAIEAAKTTTLARFLYALGIREVGEATAA
HHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAAHFASLDALRVATVEQLTAVEDVGVVVAQHVAHFFAQPHNLEVIDALIAAGVHWPAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
EAPSADAQPLKGQTWVLTGTLNQLNRNDAKAQLQTLGAKVAGSVSKNTDCLVAGEAAGSK
CCCCCCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHH
LAKAQELGVKVIDEEALLALFAANR
HHHHHHCCCEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]