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The map label for this gene is yafC [C]

Identifier: 120598139

GI number: 120598139

Start: 1498694

End: 1499707

Strand: Direct

Name: yafC [C]

Synonym: Sputw3181_1316

Alternate gene names: 120598139

Gene position: 1498694-1499707 (Clockwise)

Preceding gene: 120598138

Following gene: 120598148

Centisome position: 31.83

GC content: 40.93

Gene sequence:

>1014_bases
GTGTTTGTGCTGCGCTCTTGCATTATACCGAAAGTTAATATCATTGTCGTAAACTGCTTAAGGCTACTTTCCATGCTAGA
ACTGTTAGAACCTATTGCGATTTTTACTCATGTGGCTAGAGCTGGTAGTTTTAGTGCCGCCGCAAGAAGGCTTGGGATCT
CTAAATCTAAGGTCAGTACGCAGGTCGCCGATCTTGAACATAGACTTGGTGTTCAGTTAATACAACGTACTACTCGAAGC
TTAAGCCTGACTGAAGCTGGGCATTTATTGTATCTGCAAGGTGAAGAATTGCTTCGTGATGCAGAGCAAGCGATTGCTAG
TGTGCACAATTTAAATGATGCTACCCGCGGAGTACTTAAGGTTGGAATTTCACAATCTTTTGGTGCAATGCATATTATTC
CAGCCCTACCGTCATTTATGGCAAAACATCCTGAGCTAGAACTACAAGTCAGTTTACTTGATCATAAAGTGGATGTGGTT
AGCGAAGGATTAGATTTATTACTGACTATGTCTGAGCAGTTACCATTAGGCATGGTTGCCAGACCATTAATGAAATGTCA
GTTCCTCTTGGTTGCCTCCCCTGATTATGTTGCTAAGCACGGTGAGCCTACTCGTCCAGAACAATTAGTTGACCATAATT
GTTTAGTTTACCAAGGTGAGTGGCATGAACACAGTATGTGGCAATTTAAGAAAGGAGATGATTACTGTGAAATTGGCGTC
TCGGGTAATTTTAGAGTCGATAACGCTCCAGCATTAAAATCTGCTGCTATTAGTGGCTTGGGTGTTGTGTATTTAGCAAG
TTATTTGATGGAAGATGAGATTACTAAGGGGACGTTAGTTCCTTTACTAAAAGATTGGCAATTAACTCACCATTTACCAC
TACAAGCAGTTTATCCTCGTCGTAAACATCTTGCGCCTAAAGTGAGTGCCTTTATTGAATTTATTAAAGACCATATAGGA
AATCCACCCTATTGGGATATTCCCTATGCAGAACTGTTCAGTAAACGTCAGTAA

Upstream 100 bases:

>100_bases
AGCCAGATATAACACTCTACTTCAGCGGACTTTTTCGGTCGAAATCTCTGCAATAAATGCTTTTGCTATGAGATATTAAT
GCAATAGTGAGCGTTTAACT

Downstream 100 bases:

>100_bases
AAATGGAAACATTGTTCTTGAAATTGCCCTAATTAGTTTTGCATAAGATTGACATTCTATCTTTTTGTTTTTTATCAGTG
AGTTGAGCTTTAGGTGTCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MFVLRSCIIPKVNIIVVNCLRLLSMLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRS
LSLTEAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVV
SEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPDYVAKHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGV
SGNFRVDNAPALKSAAISGLGVVYLASYLMEDEITKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIG
NPPYWDIPYAELFSKRQ

Sequences:

>Translated_337_residues
MFVLRSCIIPKVNIIVVNCLRLLSMLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRS
LSLTEAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVV
SEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPDYVAKHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGV
SGNFRVDNAPALKSAAISGLGVVYLASYLMEDEITKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIG
NPPYWDIPYAELFSKRQ
>Mature_337_residues
MFVLRSCIIPKVNIIVVNCLRLLSMLELLEPIAIFTHVARAGSFSAAARRLGISKSKVSTQVADLEHRLGVQLIQRTTRS
LSLTEAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLKVGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVV
SEGLDLLLTMSEQLPLGMVARPLMKCQFLLVASPDYVAKHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGV
SGNFRVDNAPALKSAAISGLGVVYLASYLMEDEITKGTLVPLLKDWQLTHHLPLQAVYPRRKHLAPKVSAFIEFIKDHIG
NPPYWDIPYAELFSKRQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=30.3030303030303, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=30.1369863013699, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789440, Length=305, Percent_Identity=26.5573770491803, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=29.5302013422819, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=27.4914089347079, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=31.4685314685315, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789639, Length=301, Percent_Identity=27.2425249169435, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=25.9515570934256, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=25.8620689655172, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788706, Length=312, Percent_Identity=26.2820512820513, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788297, Length=303, Percent_Identity=26.0726072607261, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790011, Length=291, Percent_Identity=25.085910652921, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788481, Length=305, Percent_Identity=26.2295081967213, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=209, Percent_Identity=26.7942583732057, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790783, Length=293, Percent_Identity=24.5733788395904, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=26.6272189349112, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=106, Percent_Identity=38.6792452830189, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367136, Length=171, Percent_Identity=27.4853801169591, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37637; Mature: 37637

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLRSCIIPKVNIIVVNCLRLLSMLELLEPIAIFTHVARAGSFSAAARRLGISKSKVST
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
QVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLK
HHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHH
VGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVA
HCCCCCCCCEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCHHEEEEHHCCCCHHHHH
RPLMKCQFLLVASPDYVAKHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGV
HHHHHEEEEEEECCCHHHCCCCCCCHHHHHCCCEEEEECCCCCCCCEEECCCCCEEEECC
SGNFRVDNAPALKSAAISGLGVVYLASYLMEDEITKGTLVPLLKDWQLTHHLPLQAVYPR
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCHHCCCH
RKHLAPKVSAFIEFIKDHIGNPPYWDIPYAELFSKRQ
HHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MFVLRSCIIPKVNIIVVNCLRLLSMLELLEPIAIFTHVARAGSFSAAARRLGISKSKVST
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
QVADLEHRLGVQLIQRTTRSLSLTEAGHLLYLQGEELLRDAEQAIASVHNLNDATRGVLK
HHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHH
VGISQSFGAMHIIPALPSFMAKHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVA
HCCCCCCCCEEHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCHHEEEEHHCCCCHHHHH
RPLMKCQFLLVASPDYVAKHGEPTRPEQLVDHNCLVYQGEWHEHSMWQFKKGDDYCEIGV
HHHHHEEEEEEECCCHHHCCCCCCCHHHHHCCCEEEEECCCCCCCCEEECCCCCEEEECC
SGNFRVDNAPALKSAAISGLGVVYLASYLMEDEITKGTLVPLLKDWQLTHHLPLQAVYPR
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCHHCCCH
RKHLAPKVSAFIEFIKDHIGNPPYWDIPYAELFSKRQ
HHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]