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The map label for this gene is pilT [H]

Identifier: 120598148

GI number: 120598148

Start: 1507430

End: 1508467

Strand: Direct

Name: pilT [H]

Synonym: Sputw3181_1325

Alternate gene names: 120598148

Gene position: 1507430-1508467 (Clockwise)

Preceding gene: 120598139

Following gene: 120598149

Centisome position: 32.02

GC content: 44.99

Gene sequence:

>1038_bases
ATGGAAATCACTGAGTTATTAGCTTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTTGACGGTGAAGTGAGAAAAATTAACCTACCAGCGCTCGATCATCGGGGCGTACACAGCTTAGTGTACGATA
TTATGAATGATAAACAGCGCAAGGACTTTGAAGAGCATTTAGAAATTGATTTTTCGTTTGAAGTGCCCAACTTAGCCCGT
TTTCGTGTAAACGCATTTAACCAATCCCGTGGTGCCGCCGCAGTATTTCGTACCATTCCCAGTGATATATTGTCACTTGA
ACAACTTGGCGCACCGGAGATATTCAAAAAAATTGCCAGTTATCCGCGTGGTTTAGTGTTAGTCACTGGACCGACTGGCT
CGGGTAAGAGTACCACACTTGCAGCAATGGTGGATTACATCAATGAAAACCGCCATGATCATATCTTAACCATTGAAGAC
CCAATTGAATTTGTCCATCCTAATAAACAATGTTTGATTAACCAGAGGGAAGTTCATCGGCATACTCACAGTTTTAACGC
TGCCCTGAGGAGTGCTTTGCGTGAGGACCCTGACGTAATTTTAGTGGGGGAGATGCGAGATCTTGAAACCATACGTCTGG
CGATGACCGCCGCAGAAACTGGCCATTTAGTCTTCGGTACTTTACACACAACTTCAGCAGCGAAGACGATTGACCGTGTG
GTGGATGTATTTCCTGCTGGGGAGAAAGACATGGTACGCACTATGTTATCTGAATCATTACAAGCGGTTATTTCACAGAC
GCTTATCAAAAAAATTGGCGGCGGCCGTGTCGCTGCACACGAAATTATGATGGGAACACCTGCAATTCGTAACTTGATCC
GTGAGGACAAAGTCGCACAAATGTACTCTGCCATTCAAACTGGCATGGCTCATGGAATGCAGACTCTGGAGCAATGCCTG
CAAAATTTAGTTAATCGTGGCCTGATCACCCGCGAAGACGCCATGTCGAAAAGCTCAAATAAACAAGCCACATTTTAA

Upstream 100 bases:

>100_bases
CATTTTTGCGCCGCTTGGGCGATCCTACACTGGGCGATTGCGAGTCTGTCTGCTATTGTTGTCATGATGTAATTATCTAG
TTGCAAGGATATCCCAAACG

Downstream 100 bases:

>100_bases
GGATTTATAATGGATGTACGTCCGTTTTTAAAAGTCATCGTTGAGCGTAAAGCCTCTGATTTGTTTATTACCGCGGGCTT
TCCTCCCAGTGCAAAAATTG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Sequences:

>Translated_345_residues
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF
>Mature_345_residues
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=48.3180428134557, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1789723, Length=356, Percent_Identity=31.4606741573034, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786296, Length=284, Percent_Identity=33.0985915492958, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38340; Mature: 38340

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHCCHHH
KDFEEHLEIDFSFEVPNLARFRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIAS
CCHHHHCCEEEEECCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
YPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIEDPIEFVHPNKQCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHCCHHHHHH
HTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTGMAHGMQTLEQCLQNLVNRGLITREDAMSKSSNKQATF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHRGVHSLVYDIMNDKQR
CCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHCCHHH
KDFEEHLEIDFSFEVPNLARFRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIAS
CCHHHHCCEEEEECCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
YPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIEDPIEFVHPNKQCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCHHHHCCHHHHHH
HTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTGMAHGMQTLEQCLQNLVNRGLITREDAMSKSSNKQATF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]