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The map label for this gene is ubiF [H]

Identifier: 120597880

GI number: 120597880

Start: 1193917

End: 1195158

Strand: Direct

Name: ubiF [H]

Synonym: Sputw3181_1050

Alternate gene names: 120597880

Gene position: 1193917-1195158 (Clockwise)

Preceding gene: 120597871

Following gene: 120597881

Centisome position: 25.36

GC content: 46.62

Gene sequence:

>1242_bases
GTGGTCACTAAGATGAGCGAGACTAAACATGCAGTTACACAGGTCGTGTCCGATACACACTGTGGCGATGTGATCCTATT
TGATGTCATTTTAGTGGGTGGGGGGATGGTCGGTGCCGCTACCGCAATTGGACTGGCACAAATGGGGCTGAAAGTCGCGG
TCATAGAGTCTTTGGTGCCAGAGGCTTATCATGCCGAACAGCCGTTAGATGTTCGTGTATCTGCTATTAGTGTGGCTTCT
GAAGCATTACTTGAACGCTTAGGTGCTATGGACACGTTGCTTAAAATGCGTAACGCGCCTTATCTAGGCTTAGAAACTTG
GGAGCTCGATGGCTGCATCACCCAATTCCACAGTTCACAAATTGGCGCCAGCCATTTAGGCCATATAGTAGAAAACCGTT
TAATCCAACTTGCCCTGTGGCAACAAATGGCCCAATTGAATAGCATCACGTTATTTTGTCCCGATAAAGTGACTGCGTTT
GCTCAAGAAGCTGATGGCGTGAGTGTGTGCTTACAATCAAGTGTTAAACTTGTGGCTAAGTTATTGGTTGGGGCTGATGG
TGCAAACTCACAGGTCCGCCAGTGGGCGGGAATTGGTATTTCAGGTTGGGATTATGCTCAATCGGCCATGCTGATCAATA
TCAAGACTGCGCAGGGGCAGCAGGATGTGACTTGGCAGCAGTTTACGCCTAAAGGGCCACGTTCTTTATTACCTCTGCCA
GGGAATAACGCTTCGCTGGTGTGGTACGACGATGCCAATCGAATTAAACAGTTAATGCAGCTTAATCACAAACAGCTCGC
AGAGCAGATAAGAGTGCATTTCCCTGCGCGTTTGGACGCTGATTTCACCGTTGAGAATAAAGGCAGTTTTGGCCTCACTC
GTCGCCATGCTCAGCGCTATTATGCTAATAATGTGGTGATTCTAGGGGATGCCGCCCATACCATTAATCCGCTAGCGGGC
CAAGGTGTTAATCTTGGTTTTAAAGATGTTGAAGCACTTTTAGCGGTAATAAAAGAGGCGTTAGCTCAAGATCAATCTTG
GTGGAACCAGACGGTTCTTGCTCAATATGAACGTCAGCGTTACCGCGATAACCAATTAATGATGACGACTATGGACATGT
TTTACGCAGGATTTAGTAACGATATTTTGCCGCTTAAACTGTTACGTAATGGCGCGTTAAAGCTGGCTAACATCAATTCA
CCCATCAAAAAAATCGTACTTAAATACGCTATGGGGCTTTAG

Upstream 100 bases:

>100_bases
TTCCGTGATATCTTGATATAAGGCATAATCGACATGCTTTGATACTCTGTCCAACATTTTCTAGCCGTATTGGACACAAA
TATAAAGAAGGGAATAAACC

Downstream 100 bases:

>100_bases
TTATTTGGCTTTTCCCCAACAGAAGTACACGGCATTTGTTGACTTTGGCTTTGAGTGCTAAAATGCCGCGTTTTTAGCAG
CTATGGCCAGCACTGATGAT

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MVTKMSETKHAVTQVVSDTHCGDVILFDVILVGGGMVGAATAIGLAQMGLKVAVIESLVPEAYHAEQPLDVRVSAISVAS
EALLERLGAMDTLLKMRNAPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMAQLNSITLFCPDKVTAF
AQEADGVSVCLQSSVKLVAKLLVGADGANSQVRQWAGIGISGWDYAQSAMLINIKTAQGQQDVTWQQFTPKGPRSLLPLP
GNNASLVWYDDANRIKQLMQLNHKQLAEQIRVHFPARLDADFTVENKGSFGLTRRHAQRYYANNVVILGDAAHTINPLAG
QGVNLGFKDVEALLAVIKEALAQDQSWWNQTVLAQYERQRYRDNQLMMTTMDMFYAGFSNDILPLKLLRNGALKLANINS
PIKKIVLKYAMGL

Sequences:

>Translated_413_residues
MVTKMSETKHAVTQVVSDTHCGDVILFDVILVGGGMVGAATAIGLAQMGLKVAVIESLVPEAYHAEQPLDVRVSAISVAS
EALLERLGAMDTLLKMRNAPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMAQLNSITLFCPDKVTAF
AQEADGVSVCLQSSVKLVAKLLVGADGANSQVRQWAGIGISGWDYAQSAMLINIKTAQGQQDVTWQQFTPKGPRSLLPLP
GNNASLVWYDDANRIKQLMQLNHKQLAEQIRVHFPARLDADFTVENKGSFGLTRRHAQRYYANNVVILGDAAHTINPLAG
QGVNLGFKDVEALLAVIKEALAQDQSWWNQTVLAQYERQRYRDNQLMMTTMDMFYAGFSNDILPLKLLRNGALKLANINS
PIKKIVLKYAMGL
>Mature_413_residues
MVTKMSETKHAVTQVVSDTHCGDVILFDVILVGGGMVGAATAIGLAQMGLKVAVIESLVPEAYHAEQPLDVRVSAISVAS
EALLERLGAMDTLLKMRNAPYLGLETWELDGCITQFHSSQIGASHLGHIVENRLIQLALWQQMAQLNSITLFCPDKVTAF
AQEADGVSVCLQSSVKLVAKLLVGADGANSQVRQWAGIGISGWDYAQSAMLINIKTAQGQQDVTWQQFTPKGPRSLLPLP
GNNASLVWYDDANRIKQLMQLNHKQLAEQIRVHFPARLDADFTVENKGSFGLTRRHAQRYYANNVVILGDAAHTINPLAG
QGVNLGFKDVEALLAVIKEALAQDQSWWNQTVLAQYERQRYRDNQLMMTTMDMFYAGFSNDILPLKLLRNGALKLANINS
PIKKIVLKYAMGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=385, Percent_Identity=29.3506493506493, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI32967307, Length=328, Percent_Identity=29.2682926829268, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1786883, Length=386, Percent_Identity=47.6683937823834, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1789273, Length=389, Percent_Identity=39.0745501285347, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789274, Length=402, Percent_Identity=27.363184079602, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=433, Percent_Identity=26.5588914549654, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321694, Length=453, Percent_Identity=25.8278145695364, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19920760, Length=456, Percent_Identity=27.4122807017544, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 45366; Mature: 45366

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTKMSETKHAVTQVVSDTHCGDVILFDVILVGGGMVGAATAIGLAQMGLKVAVIESLVP
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
EAYHAEQPLDVRVSAISVASEALLERLGAMDTLLKMRNAPYLGLETWELDGCITQFHSSQ
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHC
IGASHLGHIVENRLIQLALWQQMAQLNSITLFCPDKVTAFAQEADGVSVCLQSSVKLVAK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
LLVGADGANSQVRQWAGIGISGWDYAQSAMLINIKTAQGQQDVTWQQFTPKGPRSLLPLP
HHHCCCCCCHHHHHHHCCCCCCCHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCC
GNNASLVWYDDANRIKQLMQLNHKQLAEQIRVHFPARLDADFTVENKGSFGLTRRHAQRY
CCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHH
YANNVVILGDAAHTINPLAGQGVNLGFKDVEALLAVIKEALAQDQSWWNQTVLAQYERQR
HHCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRDNQLMMTTMDMFYAGFSNDILPLKLLRNGALKLANINSPIKKIVLKYAMGL
HCCCCEEHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVTKMSETKHAVTQVVSDTHCGDVILFDVILVGGGMVGAATAIGLAQMGLKVAVIESLVP
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
EAYHAEQPLDVRVSAISVASEALLERLGAMDTLLKMRNAPYLGLETWELDGCITQFHSSQ
HHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHC
IGASHLGHIVENRLIQLALWQQMAQLNSITLFCPDKVTAFAQEADGVSVCLQSSVKLVAK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHH
LLVGADGANSQVRQWAGIGISGWDYAQSAMLINIKTAQGQQDVTWQQFTPKGPRSLLPLP
HHHCCCCCCHHHHHHHCCCCCCCHHCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCC
GNNASLVWYDDANRIKQLMQLNHKQLAEQIRVHFPARLDADFTVENKGSFGLTRRHAQRY
CCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHH
YANNVVILGDAAHTINPLAGQGVNLGFKDVEALLAVIKEALAQDQSWWNQTVLAQYERQR
HHCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRDNQLMMTTMDMFYAGFSNDILPLKLLRNGALKLANINSPIKKIVLKYAMGL
HCCCCEEHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]