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The map label for this gene is uvrD

Identifier: 120597399

GI number: 120597399

Start: 623046

End: 625214

Strand: Direct

Name: uvrD

Synonym: Sputw3181_0568

Alternate gene names: 120597399

Gene position: 623046-625214 (Clockwise)

Preceding gene: 120597396

Following gene: 120597400

Centisome position: 13.23

GC content: 49.75

Gene sequence:

>2169_bases
ATGGACGTATCTTCTTTACTAGACGGCCTGAATGATAAGCAGCGCGAGGCTGTCGCGGCACCGCAATCTAGCATGTTAGT
GTTGGCGGGCGCGGGCAGTGGTAAAACTCGGGTGTTGACCCACCGTATTGCGTGGCTGATGCAGGTTGAACAGCAGAGTC
CGTACTCGATTCTGGCGGTGACGTTTACCAATAAAGCTGCGGCTGAGATGCGCGAACGAGTTGAAAAAGTCGCTGGTACC
AATATGGGCCGCATGTGGATTGGCACTTTCCATGGCTTGGCACATCGCTTGTTGCGCACTCACTTCCAAGATGCGGGTTT
ACCGCAAAGCTTCCAAATCCTCGATTCTGACGATCAAGTGCGCTTGATCAAACGTATTCTTAAAAGCCTGAATTTGGATG
AGAAACAATATCCTCCACGCCAAGCTCAGGGTTATATCAATGGTAAAAAAGACCAAGGATTACGCCCTAAACATATCGAT
GCCATCGGTTTTCCCATTGAGCAAAATCTGTTAAAAATCTATCAGGTTTATCAAGAGTCTTGCGACCGCGCAGGTTTAGT
CGATTTTGCCGAAATCCTTTTACGTGCCCATGAGTTATGGCTTAATAAGCCGCATTTGTTGGCCCATTATCAAGAACGCT
TTAAGCATATTTTGGTGGACGAGTTCCAAGATACCAACGCGATCCAATATGCGTGGATCCGCGTGCTTGCCGGTCAAACT
GCCAATGTGATGATAGTCGGTGATGACGACCAATCGATTTACGGCTGGCGCGGCGCTCAGGTTGAAAACCTGCATCGCTT
CCTTCAGGATTTTCCTACCGCAACCACCATTCGCCTCGAGCAAAACTATCGCTCTAAGGGGAATATTCTTAAAGCGTCCA
ACGCGTTAATCGCCAATAACCCCGAGCGTATGGGCAAAGAGTTATGGACGGATGAAGCCGATGGCGATCCAATTTCGCTC
TATTGCGCCTTTAATGAAATGGATGAGGCGCGCTTTATTGTCGGTCGGATAAATGATTGGCATGAAAAGGGCGGTAACCT
CAGTGATTGCGCAATTTTATATCGCTCGAATGCTCAGTCGCGGGTATTGGAAGAAGCCTTATTACACAAAGGCTTAGCCT
ATCGTATTTACGGTGGCTTACGCTTTTTCGAGCGTCAAGAAATTAAAGATGCCATGGGCTATATGCGCCTCATCAGTAAT
AAAAATGATGATGCCGCCTTCGAGCGGGTGGTGAATACGCCGCCCCGTGGTATAGGCGATCGCACCTTAGATATTTTACG
CACTACGGCGCGCCAACAAGAGCTTACGTTATGGCAAGCGTGTTTAAGGCTGCTGGATGAAAAAGTACTTGCCGGCCGTG
CAGCCAGTGCGGTGCGTGGCTTTATGGATTTAATCGTTACTCTACAGGAAGATACGCAGGATATGGCCTTGTATCGCATG
GCCGACACTGTGATCCAAGCGTCGGGCCTTAAGGCCATGTATGAAGCCGAAAAGGGCGAGAAAGCCCACGCTAGAATCGA
AAACTTAGAAGAACTCGTCACGGCGGCACGTACCTTTGAAATGCCTGAAGAGCTTGAGGATATGGGCGAGCTTAACGCCT
TTTTATCCCATGCTGCGCTTGAGGCGGGTGAAGGGCAGGCCGATGCCTTTACCGATGCGGTGCAACTGATGACGCTGCAT
TCGGCCAAAGGGCTTGAGTTTACCATGGTGTTTATGGCGGGAGTCGAGGAAGGGATTTTCCCGAGTAAAATGGCGCTCGA
AGAGGGCGACAGACTCGATGAAGAACGCCGTCTCTGCTACGTGGGCATGACCCGCGCGATGGAAAAACTCTACATTACCT
ATGCGGAATCGCGCCGTATCTATGGCCGGGAAGATTACGCGCGGCCGTCGCGTTTTATCAAAGAGATCCCGCCGCAATAT
GTGGATGAAATTCGCCTTAAGGCGCAGGTTTCCACTCCCATGGCAAATAATCGTTTTAGCACTCAAAAGTCGGCAGCGGC
AAATGATACGGGCTTTAATGTTGGACAGAGAGTCAATCATCCTAAATTTGGTGAAGGCAAGGTCACCAACTTTGAAGGCA
GCGGCGCACAGGCCAGAGTTCAGGTTAGTTTTACCGACTTTGGCAGTAAATGGTTAGTGGTGGCTTACGCTCGTTTAGAA
GCGCTTTAA

Upstream 100 bases:

>100_bases
TCGTCCTATGGTTCTCGTCTAGTGGCTCTTGTATTGTGGATCAAGCGCGCAGGCGGGTTTTCTTCTGTTATAATGCCCCC
TCGATTTTTTGGAGGCGAGA

Downstream 100 bases:

>100_bases
GCTTGAAATGATGGGTGAGTGTCATTCTTTTGTGACAATGTTTTTGGGTGGGAATTCGCGTTCAGGGTTAGCTTAGGTAT
ATGATGCTAACCCATAGGGA

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MDVSSLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYSILAVTFTNKAAAEMRERVEKVAGT
NMGRMWIGTFHGLAHRLLRTHFQDAGLPQSFQILDSDDQVRLIKRILKSLNLDEKQYPPRQAQGYINGKKDQGLRPKHID
AIGFPIEQNLLKIYQVYQESCDRAGLVDFAEILLRAHELWLNKPHLLAHYQERFKHILVDEFQDTNAIQYAWIRVLAGQT
ANVMIVGDDDQSIYGWRGAQVENLHRFLQDFPTATTIRLEQNYRSKGNILKASNALIANNPERMGKELWTDEADGDPISL
YCAFNEMDEARFIVGRINDWHEKGGNLSDCAILYRSNAQSRVLEEALLHKGLAYRIYGGLRFFERQEIKDAMGYMRLISN
KNDDAAFERVVNTPPRGIGDRTLDILRTTARQQELTLWQACLRLLDEKVLAGRAASAVRGFMDLIVTLQEDTQDMALYRM
ADTVIQASGLKAMYEAEKGEKAHARIENLEELVTAARTFEMPEELEDMGELNAFLSHAALEAGEGQADAFTDAVQLMTLH
SAKGLEFTMVFMAGVEEGIFPSKMALEEGDRLDEERRLCYVGMTRAMEKLYITYAESRRIYGREDYARPSRFIKEIPPQY
VDEIRLKAQVSTPMANNRFSTQKSAAANDTGFNVGQRVNHPKFGEGKVTNFEGSGAQARVQVSFTDFGSKWLVVAYARLE
AL

Sequences:

>Translated_722_residues
MDVSSLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYSILAVTFTNKAAAEMRERVEKVAGT
NMGRMWIGTFHGLAHRLLRTHFQDAGLPQSFQILDSDDQVRLIKRILKSLNLDEKQYPPRQAQGYINGKKDQGLRPKHID
AIGFPIEQNLLKIYQVYQESCDRAGLVDFAEILLRAHELWLNKPHLLAHYQERFKHILVDEFQDTNAIQYAWIRVLAGQT
ANVMIVGDDDQSIYGWRGAQVENLHRFLQDFPTATTIRLEQNYRSKGNILKASNALIANNPERMGKELWTDEADGDPISL
YCAFNEMDEARFIVGRINDWHEKGGNLSDCAILYRSNAQSRVLEEALLHKGLAYRIYGGLRFFERQEIKDAMGYMRLISN
KNDDAAFERVVNTPPRGIGDRTLDILRTTARQQELTLWQACLRLLDEKVLAGRAASAVRGFMDLIVTLQEDTQDMALYRM
ADTVIQASGLKAMYEAEKGEKAHARIENLEELVTAARTFEMPEELEDMGELNAFLSHAALEAGEGQADAFTDAVQLMTLH
SAKGLEFTMVFMAGVEEGIFPSKMALEEGDRLDEERRLCYVGMTRAMEKLYITYAESRRIYGREDYARPSRFIKEIPPQY
VDEIRLKAQVSTPMANNRFSTQKSAAANDTGFNVGQRVNHPKFGEGKVTNFEGSGAQARVQVSFTDFGSKWLVVAYARLE
AL
>Mature_722_residues
MDVSSLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYSILAVTFTNKAAAEMRERVEKVAGT
NMGRMWIGTFHGLAHRLLRTHFQDAGLPQSFQILDSDDQVRLIKRILKSLNLDEKQYPPRQAQGYINGKKDQGLRPKHID
AIGFPIEQNLLKIYQVYQESCDRAGLVDFAEILLRAHELWLNKPHLLAHYQERFKHILVDEFQDTNAIQYAWIRVLAGQT
ANVMIVGDDDQSIYGWRGAQVENLHRFLQDFPTATTIRLEQNYRSKGNILKASNALIANNPERMGKELWTDEADGDPISL
YCAFNEMDEARFIVGRINDWHEKGGNLSDCAILYRSNAQSRVLEEALLHKGLAYRIYGGLRFFERQEIKDAMGYMRLISN
KNDDAAFERVVNTPPRGIGDRTLDILRTTARQQELTLWQACLRLLDEKVLAGRAASAVRGFMDLIVTLQEDTQDMALYRM
ADTVIQASGLKAMYEAEKGEKAHARIENLEELVTAARTFEMPEELEDMGELNAFLSHAALEAGEGQADAFTDAVQLMTLH
SAKGLEFTMVFMAGVEEGIFPSKMALEEGDRLDEERRLCYVGMTRAMEKLYITYAESRRIYGREDYARPSRFIKEIPPQY
VDEIRLKAQVSTPMANNRFSTQKSAAANDTGFNVGQRVNHPKFGEGKVTNFEGSGAQARVQVSFTDFGSKWLVVAYARLE
AL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=722, Percent_Identity=70.4986149584488, Blast_Score=1074, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=634, Percent_Identity=37.5394321766561, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787196, Length=90, Percent_Identity=44.4444444444444, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=873, Percent_Identity=26.1168384879725, Blast_Score=203, Evalue=8e-53,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81614; Mature: 81614

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSSLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYSILAV
CCHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TFTNKAAAEMRERVEKVAGTNMGRMWIGTFHGLAHRLLRTHFQDAGLPQSFQILDSDDQV
EECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
RLIKRILKSLNLDEKQYPPRQAQGYINGKKDQGLRPKHIDAIGFPIEQNLLKIYQVYQES
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
CDRAGLVDFAEILLRAHELWLNKPHLLAHYQERFKHILVDEFQDTNAIQYAWIRVLAGQT
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
ANVMIVGDDDQSIYGWRGAQVENLHRFLQDFPTATTIRLEQNYRSKGNILKASNALIANN
CEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCEEECC
PERMGKELWTDEADGDPISLYCAFNEMDEARFIVGRINDWHEKGGNLSDCAILYRSNAQS
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHCCCCCCCEEEEEECCCHH
RVLEEALLHKGLAYRIYGGLRFFERQEIKDAMGYMRLISNKNDDAAFERVVNTPPRGIGD
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCH
RTLDILRTTARQQELTLWQACLRLLDEKVLAGRAASAVRGFMDLIVTLQEDTQDMALYRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ADTVIQASGLKAMYEAEKGEKAHARIENLEELVTAARTFEMPEELEDMGELNAFLSHAAL
HHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EAGEGQADAFTDAVQLMTLHSAKGLEFTMVFMAGVEEGIFPSKMALEEGDRLDEERRLCY
HCCCCCHHHHHHHHHHHHHHCCCCCEEEEHEEHHHHCCCCCCHHHHHCCCCCCHHHHEEH
VGMTRAMEKLYITYAESRRIYGREDYARPSRFIKEIPPQYVDEIRLKAQVSTPMANNRFS
HHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCC
TQKSAAANDTGFNVGQRVNHPKFGEGKVTNFEGSGAQARVQVSFTDFGSKWLVVAYARLE
CCHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCCEEEEEHHHHH
AL
CC
>Mature Secondary Structure
MDVSSLLDGLNDKQREAVAAPQSSMLVLAGAGSGKTRVLTHRIAWLMQVEQQSPYSILAV
CCHHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
TFTNKAAAEMRERVEKVAGTNMGRMWIGTFHGLAHRLLRTHFQDAGLPQSFQILDSDDQV
EECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
RLIKRILKSLNLDEKQYPPRQAQGYINGKKDQGLRPKHIDAIGFPIEQNLLKIYQVYQES
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
CDRAGLVDFAEILLRAHELWLNKPHLLAHYQERFKHILVDEFQDTNAIQYAWIRVLAGQT
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
ANVMIVGDDDQSIYGWRGAQVENLHRFLQDFPTATTIRLEQNYRSKGNILKASNALIANN
CEEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCEEECC
PERMGKELWTDEADGDPISLYCAFNEMDEARFIVGRINDWHEKGGNLSDCAILYRSNAQS
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEECCCHHHHCCCCCCCEEEEEECCCHH
RVLEEALLHKGLAYRIYGGLRFFERQEIKDAMGYMRLISNKNDDAAFERVVNTPPRGIGD
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCH
RTLDILRTTARQQELTLWQACLRLLDEKVLAGRAASAVRGFMDLIVTLQEDTQDMALYRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ADTVIQASGLKAMYEAEKGEKAHARIENLEELVTAARTFEMPEELEDMGELNAFLSHAAL
HHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EAGEGQADAFTDAVQLMTLHSAKGLEFTMVFMAGVEEGIFPSKMALEEGDRLDEERRLCY
HCCCCCHHHHHHHHHHHHHHCCCCCEEEEHEEHHHHCCCCCCHHHHHCCCCCCHHHHEEH
VGMTRAMEKLYITYAESRRIYGREDYARPSRFIKEIPPQYVDEIRLKAQVSTPMANNRFS
HHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCC
TQKSAAANDTGFNVGQRVNHPKFGEGKVTNFEGSGAQARVQVSFTDFGSKWLVVAYARLE
CCHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCCEEEEEHHHHH
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]