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The map label for this gene is leuC [H]

Identifier: 120597207

GI number: 120597207

Start: 405487

End: 406911

Strand: Direct

Name: leuC [H]

Synonym: Sputw3181_0376

Alternate gene names: 120597207

Gene position: 405487-406911 (Clockwise)

Preceding gene: 120597206

Following gene: 120597208

Centisome position: 8.61

GC content: 51.79

Gene sequence:

>1425_bases
ATGACGACTGTGCCAAAAAACGCGGTGGCAAAAACGCTGTATCAAAAAGTATGGGATGCCCACGTTGTAGCGACTCCTGA
AGGTGAAGCGCCGATCATTTATGTCGACAGACATTTAGTCCATGAAGTGACTTCTCCCCAAGCATTCAGTGGCTTAAAAG
TGGCGGGCCGCAAACTGCGTGCACCGGAAAAAACCTTTGCTACTATGGATCACAATACTTCGACCCGTAGCGCCAGCTTG
GATGCCCTAAGCCCAATGGCGCGCACACAGGTTGAAACCTTAGCGCAAAACTGTAAAGACTTTGGCGTGCGTTTATACGA
CATTCATCATCCTAATCAAGGGATTGTGCATGTGATGGGCCCTGAGTTGGGTATTACCTTGCCGGGCACTGTGATTGTCT
GTGGTGATTCCCATACCGCGACCCATGGCGCCTTTGGCGCATTGGCGTTTGGCATTGGCACATCGGAAGTTGAGCATGTT
TTAGCAACCCAAACCCTGCGTCAGTTAAAAGCTAAGACCATGAAGATTGAAGTGCGTGGTCATGTTAGCGATGGCGTGAC
CGCCAAAGATATCGTACTCGCTATTATTGGCAAAATCGGTATGGATGGCGGCACGGGTTATGTGGTGGAGTTCTGTGGTG
AGGCGATTGAAGCCCTGTCGATGGAAGGCCGAATGACAGTGTGCAACATGGCGATCGAGATGGGCGCTAAGGCGGGCATG
GTCGCGCCGGATCAAACAACTTTCGACTATTTAGCAGGCCGTGAGTTTGCACCCAAGGGCGAAGATTGGGCAGAGGCTGT
CGCTTACTGGCAAGCCATAAAAACCGATGATGGCGCCGTATTTGATGCTGTCGTTGAATTAGATGCCGCTGATATCGCAC
CGCAATTGACTTGGGGCACTAACCCCGGCCAAGTCGTAGCGATCGATGGTAAAGTGCCTAATCCTCTTGATGAAGCCAAC
CCAAGCACCCGTGCCAGTATGGAGAAGGCTTTAGAATACATTGGCCTGAGTGCGGGCACGCCGATGACAGACATCAGTAT
CAACAAGGTATTTATCGGCTCTTGCACTAACTCACGGATTGAAGATTTACGCAGCGCCGCCGTACATGCTAAAGGCCGCA
AAGTCGCGAGTGGCGTCACCGCGATTGTCGTACCTGGTTCTGGTCAAGTGAAAGCCCAAGCCGAAGCCGAAGGCTTAGAT
AAAATCTTTATCGAAGCGGGATTTGAGTGGCGTTTACCGGGTTGCTCTATGTGTTTAGCGATGAACGATGACAGATTAGA
AGCGGGTGATCGCTGCGCTTCGACCAGTAATCGTAATTTCGAAGGTCGCCAAGGCCGCGGCAGTCGCACCCATTTAGTGA
GCCCTGCAATGGCCGCCGCCGCTGCGGTGGCTGGGCATTTTGTCGATATTCGTAAACCTTACTAA

Upstream 100 bases:

>100_bases
ACTTAACTGGTGAGCTATTGAGCAGCGACAAGCGTAGCCAAGCTAAATCAACCGCCGAGATGGGTGACTTTATCGCCAAT
GCTATAAAGGAAGGTGTGTA

Downstream 100 bases:

>100_bases
TTACCGTAAACCCAAGAGGAGAAGTTAATGCAACCTTTTACGACCCATACCGGGCTTGCGGTCATGATCGACAGCACCAA
TATCGATACGGATCAGATCA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTTVPKNAVAKTLYQKVWDAHVVATPEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASL
DALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHV
LATQTLRQLKAKTMKIEVRGHVSDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGM
VAPDQTTFDYLAGREFAPKGEDWAEAVAYWQAIKTDDGAVFDAVVELDAADIAPQLTWGTNPGQVVAIDGKVPNPLDEAN
PSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLD
KIFIEAGFEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Sequences:

>Translated_474_residues
MTTVPKNAVAKTLYQKVWDAHVVATPEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASL
DALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHV
LATQTLRQLKAKTMKIEVRGHVSDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGM
VAPDQTTFDYLAGREFAPKGEDWAEAVAYWQAIKTDDGAVFDAVVELDAADIAPQLTWGTNPGQVVAIDGKVPNPLDEAN
PSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLD
KIFIEAGFEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
>Mature_473_residues
TTVPKNAVAKTLYQKVWDAHVVATPEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTRSASLD
ALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVL
ATQTLRQLKAKTMKIEVRGHVSDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMV
APDQTTFDYLAGREFAPKGEDWAEAVAYWQAIKTDDGAVFDAVVELDAADIAPQLTWGTNPGQVVAIDGKVPNPLDEANP
STRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRIEDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDK
IFIEAGFEWRLPGCSMCLAMNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=29.8913043478261, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI8659555, Length=418, Percent_Identity=27.511961722488, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=26.7716535433071, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=73.0603448275862, Blast_Score=708, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=29.1553133514986, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=25.1461988304094, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI2367097, Length=437, Percent_Identity=24.2562929061785, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=383, Percent_Identity=28.4595300261097, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=383, Percent_Identity=28.4595300261097, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=28.5245901639344, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=448, Percent_Identity=27.2321428571429, Blast_Score=105, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=62.046908315565, Blast_Score=580, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6320440, Length=465, Percent_Identity=26.0215053763441, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=371, Percent_Identity=29.9191374663073, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=398, Percent_Identity=28.391959798995, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281365315, Length=383, Percent_Identity=27.154046997389, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17864292, Length=383, Percent_Identity=27.154046997389, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=26.5984654731458, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28571643, Length=383, Percent_Identity=27.9373368146214, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24645686, Length=456, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137564, Length=428, Percent_Identity=25, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50359; Mature: 50228

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVPKNAVAKTLYQKVWDAHVVATPEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLR
CCCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHCC
APEKTFATMDHNTSTRSASLDALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMG
CCCHHHEECCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEC
PELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRG
CCCCEECCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEE
HVSDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGM
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
VAPDQTTFDYLAGREFAPKGEDWAEAVAYWQAIKTDDGAVFDAVVELDAADIAPQLTWGT
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCEEECC
NPGQVVAIDGKVPNPLDEANPSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRI
CCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECEEEEECCCCHHH
EDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLA
HHHHHHHHHHCCCEECCCCEEEEECCCCCEEECHHHCCCCEEEEECCCEEECCCCEEEEE
MNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
ECCCCCCCCCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
TTVPKNAVAKTLYQKVWDAHVVATPEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLR
CCCCHHHHHHHHHHHHHCCEEEECCCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHCC
APEKTFATMDHNTSTRSASLDALSPMARTQVETLAQNCKDFGVRLYDIHHPNQGIVHVMG
CCCHHHEECCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEEC
PELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQLKAKTMKIEVRG
CCCCEECCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEE
HVSDGVTAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGM
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
VAPDQTTFDYLAGREFAPKGEDWAEAVAYWQAIKTDDGAVFDAVVELDAADIAPQLTWGT
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCEEECC
NPGQVVAIDGKVPNPLDEANPSTRASMEKALEYIGLSAGTPMTDISINKVFIGSCTNSRI
CCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECEEEEECCCCHHH
EDLRSAAVHAKGRKVASGVTAIVVPGSGQVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLA
HHHHHHHHHHCCCEECCCCEEEEECCCCCEEECHHHCCCCEEEEECCCEEECCCCEEEEE
MNDDRLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
ECCCCCCCCCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]