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The map label for this gene is leuD

Identifier: 120597208

GI number: 120597208

Start: 406939

End: 407544

Strand: Direct

Name: leuD

Synonym: Sputw3181_0377

Alternate gene names: 120597208

Gene position: 406939-407544 (Clockwise)

Preceding gene: 120597207

Following gene: 120597209

Centisome position: 8.64

GC content: 49.67

Gene sequence:

>606_bases
ATGCAACCTTTTACGACCCATACCGGGCTTGCGGTCATGATCGACAGCACCAATATCGATACGGATCAGATCATTCCGAA
ACAGTTTTTGTCTAAAGTGACGCGCGATGGTTTTGGCGTGCACTTATTTCATGACTGGCGTTATTTAGATGATGCGGGCG
ATGTGCCCAATCCTGAGTTTTCATTGAACCAGCCTCGTTATAAAGGCGCTTCAATTCTTGTGTCGCAGGAAAACTTTGGT
TGTGGTTCAAGCCGCGAACATGCGCCTTGGGCGCTGGCGGACTTTGGTTTGCGCGCCATTATAGCGCCGAGTTTTGCCGA
CATTTTTTACGGCAACTCAATCAATAATGGCCTATTGCCTGTGGCGTTAACCCATGCGCAGGTGCGTCAGTTGATGGATG
AAGTGGCGGCAGAAGAAGGTGCGCAAATCACGGTCGATCTCACAAGCTGTAAAGTGATTTCACCGTCAGGTGCCGAGTTT
AGTTTTACGCTGGCAGAGTCGGCGAGACATAAGTTACTCAATGGGTTAGATGCCATTGGGCTGACCTTGTCCCATGCTGC
GCAAATCAGTCAGTACGAAACCCAAATCCAAGGCTGGCGGAGTTAA

Upstream 100 bases:

>100_bases
TTTAGTGAGCCCTGCAATGGCCGCCGCCGCTGCGGTGGCTGGGCATTTTGTCGATATTCGTAAACCTTACTAATTACCGT
AAACCCAAGAGGAGAAGTTA

Downstream 100 bases:

>100_bases
AATTTCAGCGAACACTTGTACCTATGATGATGCAATAATAAAATGCCTTGACGAAAGTCTAGGCGTTTTTGTTAGCACAT
TTGTTGAGCCTTAATTTAAG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDVPNPEFSLNQPRYKGASILVSQENFG
CGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLPVALTHAQVRQLMDEVAAEEGAQITVDLTSCKVISPSGAEF
SFTLAESARHKLLNGLDAIGLTLSHAAQISQYETQIQGWRS

Sequences:

>Translated_201_residues
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDVPNPEFSLNQPRYKGASILVSQENFG
CGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLPVALTHAQVRQLMDEVAAEEGAQITVDLTSCKVISPSGAEF
SFTLAESARHKLLNGLDAIGLTLSHAAQISQYETQIQGWRS
>Mature_201_residues
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDVPNPEFSLNQPRYKGASILVSQENFG
CGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLPVALTHAQVRQLMDEVAAEEGAQITVDLTSCKVISPSGAEF
SFTLAESARHKLLNGLDAIGLTLSHAAQISQYETQIQGWRS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=57.7319587628866, Blast_Score=237, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=45.6852791878173, Blast_Score=174, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21978; Mature: 21978

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDVPNPEF
CCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCC
SLNQPRYKGASILVSQENFGCGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLP
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCHHHHHCCCHHHHHCCCCCCCCCCC
VALTHAQVRQLMDEVAAEEGAQITVDLTSCKVISPSGAEFSFTLAESARHKLLNGLDAIG
EEECHHHHHHHHHHHHHCCCCEEEEEECEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
LTLSHAAQISQYETQIQGWRS
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQPFTTHTGLAVMIDSTNIDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDVPNPEF
CCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCC
SLNQPRYKGASILVSQENFGCGSSREHAPWALADFGLRAIIAPSFADIFYGNSINNGLLP
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCHHHHHCCCHHHHHCCCCCCCCCCC
VALTHAQVRQLMDEVAAEEGAQITVDLTSCKVISPSGAEFSFTLAESARHKLLNGLDAIG
EEECHHHHHHHHHHHHHCCCCEEEEEECEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHH
LTLSHAAQISQYETQIQGWRS
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA