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The map label for this gene is hsdS [C]

Identifier: 120597149

GI number: 120597149

Start: 344670

End: 345923

Strand: Direct

Name: hsdS [C]

Synonym: Sputw3181_0318

Alternate gene names: 120597149

Gene position: 344670-345923 (Clockwise)

Preceding gene: 120597148

Following gene: 120597150

Centisome position: 7.32

GC content: 42.19

Gene sequence:

>1254_bases
ATGAGTGAGCGGGTTCCAGATGGTTGGATGTTAAAAATTGTTAGAGATACTTCTAAATTATCAGCAGGAGGAACACCTAG
CACGCAAGTAACTGAATATTGGGAGAATGGTACTATTCCATGGATGTCTTCTGGAGAAGTGCACAAAAAACGCGTGCATA
GTGTTGATAATTGTATAACAACGCTCGGCTTAGAAAATAGCAGTGCGAAAATGTTTCCTTCAAAGTCTATATTGGTGGCT
CTAGCTGGACAGGGGAAAACGCGTGGAACGGTTGCTATCAGTGAGATAGAGTTAACGACAAATCAATCTATTGCTGCAAT
TATTGTCAAGGATAAATCAGTTTATCCCGATTTTTTGTATCACAATCTTGATAGCCGCTATGAAGAGTTAAGAGGGGTCT
CTGGGGGAAGCGGTCGCGCAGGGTTAAACCTTGCGATATTAGGTGATCTAGATGTTTTGTTACCACCACTCCCTGAACAA
CAAAAAATCGCCAAGATTCTGACCTCGGTGGATCAGGTGATTGAGAAGACACAGGCACAAATCGACAAGCTCAAAGACCT
GAAAACCGGCATGATGCAGGAGCTGTTAACCCAAGGTGTCGGCGTCGATGGCAAACCTCACACTGAGTTTAAGGATTCCC
CTGTCGGCTGGATTCCTAAGACGTGGGATTTAGAGCCTCTCGCAAATTTCACGACATTTATATCTTATGGGTTTACAAAC
CCAATGCCTGAGGCCGAAGTTGGCCCATATATGATCACGGCTAAAGATGTGAATGACCTCAAGGTTCAGTACAGTACGTC
GCGTAAAACTACCCAAGAAGCTTTTGATAATTTGCTTACTCGTAAAAGTCGCCCACAGGTCAATGATATCTTGTTGACCA
AAGATGGAACATTGGGGCGCGTTGCTTTAGTAACTGACAGTAATTGTTGCATCAATCAATCTGTTGCGGTTTTAACACCA
AATGAGCGAGTAATCCCTAAGTTTCTTCTCTATCTACTTGCTTCACCCCGATATCAGCAAGAGATGTTAGAAAATGCTGG
TGGTTCAACCATCAAGCATATTTACATAACCGTAGTAGACAAAATGTTGGTTGGGGTTCCTTCGGTCACTGAGCAACAAA
AGTTAGTAGATATTTTTGATAGTGTTTTTCGAAAATTAGAATTGACTGAAAATAAGTTGTCAAAACTGAATGATACTAAA
AAAGCCCTGATGCAGGATCTGTTAACGGGTAAGGTGCGGGTAAATATCGACTAG

Upstream 100 bases:

>100_bases
GAGCGCTGGTGGGACAAGTACCACGTATCACTCAAGCAACTCGATGATGAAGTCGCAACCGCCGAGAGCGTGATGCAAGG
CTATCTCAAGGAGCTGGGTT

Downstream 100 bases:

>100_bases
CAAGGACGGGTAATGTTACAGGCAATTCTCCATGGAAAGGCGGGACGTATCGACGGCGACAAAGTCCAGTCGATAAGTTG
GCGTGAAGTATTCAAGGGAC

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSERVPDGWMLKIVRDTSKLSAGGTPSTQVTEYWENGTIPWMSSGEVHKKRVHSVDNCITTLGLENSSAKMFPSKSILVA
LAGQGKTRGTVAISEIELTTNQSIAAIIVKDKSVYPDFLYHNLDSRYEELRGVSGGSGRAGLNLAILGDLDVLLPPLPEQ
QKIAKILTSVDQVIEKTQAQIDKLKDLKTGMMQELLTQGVGVDGKPHTEFKDSPVGWIPKTWDLEPLANFTTFISYGFTN
PMPEAEVGPYMITAKDVNDLKVQYSTSRKTTQEAFDNLLTRKSRPQVNDILLTKDGTLGRVALVTDSNCCINQSVAVLTP
NERVIPKFLLYLLASPRYQQEMLENAGGSTIKHIYITVVDKMLVGVPSVTEQQKLVDIFDSVFRKLELTENKLSKLNDTK
KALMQDLLTGKVRVNID

Sequences:

>Translated_417_residues
MSERVPDGWMLKIVRDTSKLSAGGTPSTQVTEYWENGTIPWMSSGEVHKKRVHSVDNCITTLGLENSSAKMFPSKSILVA
LAGQGKTRGTVAISEIELTTNQSIAAIIVKDKSVYPDFLYHNLDSRYEELRGVSGGSGRAGLNLAILGDLDVLLPPLPEQ
QKIAKILTSVDQVIEKTQAQIDKLKDLKTGMMQELLTQGVGVDGKPHTEFKDSPVGWIPKTWDLEPLANFTTFISYGFTN
PMPEAEVGPYMITAKDVNDLKVQYSTSRKTTQEAFDNLLTRKSRPQVNDILLTKDGTLGRVALVTDSNCCINQSVAVLTP
NERVIPKFLLYLLASPRYQQEMLENAGGSTIKHIYITVVDKMLVGVPSVTEQQKLVDIFDSVFRKLELTENKLSKLNDTK
KALMQDLLTGKVRVNID
>Mature_416_residues
SERVPDGWMLKIVRDTSKLSAGGTPSTQVTEYWENGTIPWMSSGEVHKKRVHSVDNCITTLGLENSSAKMFPSKSILVAL
AGQGKTRGTVAISEIELTTNQSIAAIIVKDKSVYPDFLYHNLDSRYEELRGVSGGSGRAGLNLAILGDLDVLLPPLPEQQ
KIAKILTSVDQVIEKTQAQIDKLKDLKTGMMQELLTQGVGVDGKPHTEFKDSPVGWIPKTWDLEPLANFTTFISYGFTNP
MPEAEVGPYMITAKDVNDLKVQYSTSRKTTQEAFDNLLTRKSRPQVNDILLTKDGTLGRVALVTDSNCCINQSVAVLTPN
ERVIPKFLLYLLASPRYQQEMLENAGGSTIKHIYITVVDKMLVGVPSVTEQQKLVDIFDSVFRKLELTENKLSKLNDTKK
ALMQDLLTGKVRVNID

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 46131; Mature: 45999

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERVPDGWMLKIVRDTSKLSAGGTPSTQVTEYWENGTIPWMSSGEVHKKRVHSVDNCIT
CCCCCCCCCEEEEEEHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
TLGLENSSAKMFPSKSILVALAGQGKTRGTVAISEIELTTNQSIAAIIVKDKSVYPDFLY
HHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHH
HNLDSRYEELRGVSGGSGRAGLNLAILGDLDVLLPPLPEQQKIAKILTSVDQVIEKTQAQ
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IDKLKDLKTGMMQELLTQGVGVDGKPHTEFKDSPVGWIPKTWDLEPLANFTTFISYGFTN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PMPEAEVGPYMITAKDVNDLKVQYSTSRKTTQEAFDNLLTRKSRPQVNDILLTKDGTLGR
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCE
VALVTDSNCCINQSVAVLTPNERVIPKFLLYLLASPRYQQEMLENAGGSTIKHIYITVVD
EEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHH
KMLVGVPSVTEQQKLVDIFDSVFRKLELTENKLSKLNDTKKALMQDLLTGKVRVNID
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SERVPDGWMLKIVRDTSKLSAGGTPSTQVTEYWENGTIPWMSSGEVHKKRVHSVDNCIT
CCCCCCCCEEEEEEHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
TLGLENSSAKMFPSKSILVALAGQGKTRGTVAISEIELTTNQSIAAIIVKDKSVYPDFLY
HHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHH
HNLDSRYEELRGVSGGSGRAGLNLAILGDLDVLLPPLPEQQKIAKILTSVDQVIEKTQAQ
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IDKLKDLKTGMMQELLTQGVGVDGKPHTEFKDSPVGWIPKTWDLEPLANFTTFISYGFTN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PMPEAEVGPYMITAKDVNDLKVQYSTSRKTTQEAFDNLLTRKSRPQVNDILLTKDGTLGR
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCE
VALVTDSNCCINQSVAVLTPNERVIPKFLLYLLASPRYQQEMLENAGGSTIKHIYITVVD
EEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHH
KMLVGVPSVTEQQKLVDIFDSVFRKLELTENKLSKLNDTKKALMQDLLTGKVRVNID
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]