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The map label for this gene is hsdM [C]

Identifier: 120597148

GI number: 120597148

Start: 342953

End: 344677

Strand: Direct

Name: hsdM [C]

Synonym: Sputw3181_0317

Alternate gene names: 120597148

Gene position: 342953-344677 (Clockwise)

Preceding gene: 120597147

Following gene: 120597149

Centisome position: 7.28

GC content: 52.12

Gene sequence:

>1725_bases
ATGACCCAGAAACTCACTCTCCAACAGCTTGAATCCTTCCTGTGGGAAGCCGCTGATATCCTGCGCGGCAATATGGACGC
CTCAGAATACAAAGACTACATCTTCGGCATGATGTTCTTAAAACGCCTGTCTGATGCTTTCGAAGAAGCGCAAGAAAGCG
TGGTGCAGTATTATCTCGATAAAGGCAAAACCCAAGAGCAGGCCGAGGCGCTGGCGCAAGATGAAGACGAATACGATAAA
ACCTTCTTTGTGCCGCAAACGGCCCGTTGGTCAGAGCTGAAAGACCTTAAACACGATATCGGTGCCACGCTCAACAAAGC
CACCGAAGCGATTGAAGAGTACAACAGCAGCCTCGAAGGGGTGTTGGTCACTATCGACTTCAACATTAAAAACAAGCTGT
CTGATAAAAAGCTGCGCGACTTGCTGTCGCATTTCAGCAAATACCGCCTGCGTAATGAAGATTTTGAGCGGCCAGACTTG
CTCGGCACCGCCTATGAATACCTCATCAAAATGTTTGCCGACAGCGCGGGTAAAAAGGGCGGTGAGTTCTACACCCCAAG
TGAAGTGGTATCGCTGCTGGTGGCTTTACTCAAGCCTAAAGCGGGCATGCGTATCTACGACCCAACCTCCGGCTCCGGCG
GTATGTTGGTGCAAACCCGTAACTACCTAGCAGCCCATGGCGAGAACCCCGGCAACCTGTCGCTGTTCGGCCAAGAGATG
AACCTAAACACATGGGCCATCTGTAAGATGAACATGTTCCTGCATGGCGTGCTCAGTGCCGATATTCGTAAGGGCGATAC
CCTGCGCGAGCCTAAGCATACCGAAGGCGGCGAGCTTATGGCCTTTGACCGCGTGATTGCCAACCCGCCATTCTCACTGG
CGAAATGGGGCAAGGATGAATGCGATAACGACGGCTTTGGCCGTTTCCCCTATGGCACTCCGCCAAAAGATGCCGGTGAC
TTAGCCTTTGTGCAGCACATGATTGCCAGCACTAATGCCGAGGGTATGGTTGGCGTGGTGATGCCGCACGGCGTGCTATT
TCGTGGCTCCAGTGAAAAGGCGATCCGTCAGGGCATTCTAGAAGATGACTTGCTGGAAGCAGTCATTGGTCTGCCATCTG
GCTTGTTCTATGGCACCAGTATTCCTGCTTGCCTACTAATTATCAATAAACGCAAAGCGGCTGACCGTAAAGGCAAGGTG
CTGTTTATCAATGGCGAGCTGGAGTACGAAGAGGGCAAAAATCAAAACAAACTGCGCCCGCAAGATATTGAGAAAATCGT
CACCACCTTTAACAACTACCAAAACATCAAGCGTTACGCCCAAGTGGTCGACATTAGCGAGATAGCTGACAACGACTACA
ACCTCAATATTCGCCGTTACGTCGATACCGCGCCACCAGCAGAGATCTTTGATGTCCGCGCCATCCTCCATGGCGGCGTG
CCCGTGCGCGAGGTCGAGAGCGAATACATTCAAGAAGAGATCCTGCAAGGCTTCGATGTCAGTAGCGTGTTTACGCTGAA
GTCCGAAGGCTACTATCAGTTCCGCACCGATATCAGCGCCAAAGAGCAGATCCGCGAGTTTGCCACGGATGCCACATCCG
CCATCATCAGCCAATTAGAGCGCTGGTGGGACAAGTACCACGTATCACTCAAGCAACTCGATGATGAAGTCGCAACCGCC
GAGAGCGTGATGCAAGGCTATCTCAAGGAGCTGGGTTATGAGTGA

Upstream 100 bases:

>100_bases
GCCCTACAATTAACAGACTGAAATCGATACATTTCACACATCCAATTTGGTTCGTAGGTGTTAAGATACGCCCAGAATCA
TTTTAAGGTTGCATTGTTCA

Downstream 100 bases:

>100_bases
GCGGGTTCCAGATGGTTGGATGTTAAAAATTGTTAGAGATACTTCTAAATTATCAGCAGGAGGAACACCTAGCACGCAAG
TAACTGAATATTGGGAGAAT

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MTQKLTLQQLESFLWEAADILRGNMDASEYKDYIFGMMFLKRLSDAFEEAQESVVQYYLDKGKTQEQAEALAQDEDEYDK
TFFVPQTARWSELKDLKHDIGATLNKATEAIEEYNSSLEGVLVTIDFNIKNKLSDKKLRDLLSHFSKYRLRNEDFERPDL
LGTAYEYLIKMFADSAGKKGGEFYTPSEVVSLLVALLKPKAGMRIYDPTSGSGGMLVQTRNYLAAHGENPGNLSLFGQEM
NLNTWAICKMNMFLHGVLSADIRKGDTLREPKHTEGGELMAFDRVIANPPFSLAKWGKDECDNDGFGRFPYGTPPKDAGD
LAFVQHMIASTNAEGMVGVVMPHGVLFRGSSEKAIRQGILEDDLLEAVIGLPSGLFYGTSIPACLLIINKRKAADRKGKV
LFINGELEYEEGKNQNKLRPQDIEKIVTTFNNYQNIKRYAQVVDISEIADNDYNLNIRRYVDTAPPAEIFDVRAILHGGV
PVREVESEYIQEEILQGFDVSSVFTLKSEGYYQFRTDISAKEQIREFATDATSAIISQLERWWDKYHVSLKQLDDEVATA
ESVMQGYLKELGYE

Sequences:

>Translated_574_residues
MTQKLTLQQLESFLWEAADILRGNMDASEYKDYIFGMMFLKRLSDAFEEAQESVVQYYLDKGKTQEQAEALAQDEDEYDK
TFFVPQTARWSELKDLKHDIGATLNKATEAIEEYNSSLEGVLVTIDFNIKNKLSDKKLRDLLSHFSKYRLRNEDFERPDL
LGTAYEYLIKMFADSAGKKGGEFYTPSEVVSLLVALLKPKAGMRIYDPTSGSGGMLVQTRNYLAAHGENPGNLSLFGQEM
NLNTWAICKMNMFLHGVLSADIRKGDTLREPKHTEGGELMAFDRVIANPPFSLAKWGKDECDNDGFGRFPYGTPPKDAGD
LAFVQHMIASTNAEGMVGVVMPHGVLFRGSSEKAIRQGILEDDLLEAVIGLPSGLFYGTSIPACLLIINKRKAADRKGKV
LFINGELEYEEGKNQNKLRPQDIEKIVTTFNNYQNIKRYAQVVDISEIADNDYNLNIRRYVDTAPPAEIFDVRAILHGGV
PVREVESEYIQEEILQGFDVSSVFTLKSEGYYQFRTDISAKEQIREFATDATSAIISQLERWWDKYHVSLKQLDDEVATA
ESVMQGYLKELGYE
>Mature_573_residues
TQKLTLQQLESFLWEAADILRGNMDASEYKDYIFGMMFLKRLSDAFEEAQESVVQYYLDKGKTQEQAEALAQDEDEYDKT
FFVPQTARWSELKDLKHDIGATLNKATEAIEEYNSSLEGVLVTIDFNIKNKLSDKKLRDLLSHFSKYRLRNEDFERPDLL
GTAYEYLIKMFADSAGKKGGEFYTPSEVVSLLVALLKPKAGMRIYDPTSGSGGMLVQTRNYLAAHGENPGNLSLFGQEMN
LNTWAICKMNMFLHGVLSADIRKGDTLREPKHTEGGELMAFDRVIANPPFSLAKWGKDECDNDGFGRFPYGTPPKDAGDL
AFVQHMIASTNAEGMVGVVMPHGVLFRGSSEKAIRQGILEDDLLEAVIGLPSGLFYGTSIPACLLIINKRKAADRKGKVL
FINGELEYEEGKNQNKLRPQDIEKIVTTFNNYQNIKRYAQVVDISEIADNDYNLNIRRYVDTAPPAEIFDVRAILHGGVP
VREVESEYIQEEILQGFDVSSVFTLKSEGYYQFRTDISAKEQIREFATDATSAIISQLERWWDKYHVSLKQLDDEVATAE
SVMQGYLKELGYE

Specific function: The M And S Subunits Together Form A Methyltransferase (Mtase) That Methylates Two Adenine Residues In Complementary Strands Of A Bipartite DNA Recognition Sequence. In The Presence Of The R Subunit The Complex Can Also Act As An Endonuclease, Binding To

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=408, Percent_Identity=23.0392156862745, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011991 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 64876; Mature: 64745

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLTLQQLESFLWEAADILRGNMDASEYKDYIFGMMFLKRLSDAFEEAQESVVQYYLD
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGKTQEQAEALAQDEDEYDKTFFVPQTARWSELKDLKHDIGATLNKATEAIEEYNSSLEG
CCCCHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLVTIDFNIKNKLSDKKLRDLLSHFSKYRLRNEDFERPDLLGTAYEYLIKMFADSAGKKG
EEEEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GEFYTPSEVVSLLVALLKPKAGMRIYDPTSGSGGMLVQTRNYLAAHGENPGNLSLFGQEM
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCHHHCCCCCCCCEEEEECCC
NLNTWAICKMNMFLHGVLSADIRKGDTLREPKHTEGGELMAFDRVIANPPFSLAKWGKDE
CCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHCCCCHHHHHCCCCC
CDNDGFGRFPYGTPPKDAGDLAFVQHMIASTNAEGMVGVVMPHGVLFRGSSEKAIRQGIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCHHHHHHCCC
EDDLLEAVIGLPSGLFYGTSIPACLLIINKRKAADRKGKVLFINGELEYEEGKNQNKLRP
HHHHHHHHHCCCCCCCCCCCCCEEHEEEECCCCCCCCCCEEEEECEEEECCCCCCCCCCH
QDIEKIVTTFNNYQNIKRYAQVVDISEIADNDYNLNIRRYVDTAPPAEIFDVRAILHGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PVREVESEYIQEEILQGFDVSSVFTLKSEGYYQFRTDISAKEQIREFATDATSAIISQLE
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
RWWDKYHVSLKQLDDEVATAESVMQGYLKELGYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQKLTLQQLESFLWEAADILRGNMDASEYKDYIFGMMFLKRLSDAFEEAQESVVQYYLD
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGKTQEQAEALAQDEDEYDKTFFVPQTARWSELKDLKHDIGATLNKATEAIEEYNSSLEG
CCCCHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VLVTIDFNIKNKLSDKKLRDLLSHFSKYRLRNEDFERPDLLGTAYEYLIKMFADSAGKKG
EEEEEEECCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GEFYTPSEVVSLLVALLKPKAGMRIYDPTSGSGGMLVQTRNYLAAHGENPGNLSLFGQEM
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCHHHCCCCCCCCEEEEECCC
NLNTWAICKMNMFLHGVLSADIRKGDTLREPKHTEGGELMAFDRVIANPPFSLAKWGKDE
CCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHCCCCHHHHHCCCCC
CDNDGFGRFPYGTPPKDAGDLAFVQHMIASTNAEGMVGVVMPHGVLFRGSSEKAIRQGIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCHHHHHHCCC
EDDLLEAVIGLPSGLFYGTSIPACLLIINKRKAADRKGKVLFINGELEYEEGKNQNKLRP
HHHHHHHHHCCCCCCCCCCCCCEEHEEEECCCCCCCCCCEEEEECEEEECCCCCCCCCCH
QDIEKIVTTFNNYQNIKRYAQVVDISEIADNDYNLNIRRYVDTAPPAEIFDVRAILHGGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PVREVESEYIQEEILQGFDVSSVFTLKSEGYYQFRTDISAKEQIREFATDATSAIISQLE
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
RWWDKYHVSLKQLDDEVATAESVMQGYLKELGYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]