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The map label for this gene is basR [H]

Identifier: 120596948

GI number: 120596948

Start: 141538

End: 142215

Strand: Direct

Name: basR [H]

Synonym: Sputw3181_0115

Alternate gene names: 120596948

Gene position: 141538-142215 (Clockwise)

Preceding gene: 120596947

Following gene: 120596949

Centisome position: 3.01

GC content: 47.05

Gene sequence:

>678_bases
ATGTTGGTATTACTGGTTGAAGACAATCGGCTTCTCTCTAACAATATCATTCAATATCTTGAATTGAGTGGCATAGAGTG
CGATTACGCGTTCAACCTAGCACAAGCGGATATGCTCATTAGTCAGCAGCAGTTCGATGCTATCATTCTTGACCTTAATT
TACCGGACGGTGATGGCATTGAAGCGTGTGAACGCTGGAAAGCGCAATGTTTTACTTCACCTATCATCATGCTAACCGCC
CGTAGCAGTTTGAATGAGCGCTTGGCTGGTTTTGCCGTTGGCGCTGATGATTATTTAATAAAACCCTTTGCGATGGAGGA
ACTGGTCGCACGATTAAAAGTGGTCGCTCAGCGTCGTCCCGCGCCCCAACGGTTGATTATTGGCGACCTCGAAATCGATT
TTGCAAATCACATGGTGTATCGGCAAGGGCAACTGCTGGTTTTGTCTAAAACCGGTTGGCAGATCTTGGCGTTGTTAGCA
AGGCGCAGCCCAGAAACAGTGAGTAGAGAAGAGATTGAACGAATGCTTTGGCCCGACAGCGCACCTGATAGTGACAGTTT
GCGCAGTCATATTCATCTTTTGCGCCGAGTGATCGATCGCCCCTTTGAGCGGCAAATATTACATACGATTCGTGGTGTCG
GTTTAAGTCTTAGGGATGAAATCAATGCAAGCGCCTAA

Upstream 100 bases:

>100_bases
GAGGCGTTGAATACCATATGGAATCAATGCGTAGGAAGTTTGGGGCGACGAATCGAGCAAACTTAATTCATCTCGCCCAT
CAATATGAGTTGATCTAAAT

Downstream 100 bases:

>100_bases
ACTTAGCCTCAAGCGTAGCTATCAAGTGTGGGGCGTTATTTTTCTATTAATAACCTTAGGTGTGAATAGTTTTATCCCGC
TTTGCATGATGTGTGCAGGT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLVLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQADMLISQQQFDAIILDLNLPDGDGIEACERWKAQCFTSPIIMLTA
RSSLNERLAGFAVGADDYLIKPFAMEELVARLKVVAQRRPAPQRLIIGDLEIDFANHMVYRQGQLLVLSKTGWQILALLA
RRSPETVSREEIERMLWPDSAPDSDSLRSHIHLLRRVIDRPFERQILHTIRGVGLSLRDEINASA

Sequences:

>Translated_225_residues
MLVLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQADMLISQQQFDAIILDLNLPDGDGIEACERWKAQCFTSPIIMLTA
RSSLNERLAGFAVGADDYLIKPFAMEELVARLKVVAQRRPAPQRLIIGDLEIDFANHMVYRQGQLLVLSKTGWQILALLA
RRSPETVSREEIERMLWPDSAPDSDSLRSHIHLLRRVIDRPFERQILHTIRGVGLSLRDEINASA
>Mature_225_residues
MLVLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQADMLISQQQFDAIILDLNLPDGDGIEACERWKAQCFTSPIIMLTA
RSSLNERLAGFAVGADDYLIKPFAMEELVARLKVVAQRRPAPQRLIIGDLEIDFANHMVYRQGQLLVLSKTGWQILALLA
RRSPETVSREEIERMLWPDSAPDSDSLRSHIHLLRRVIDRPFERQILHTIRGVGLSLRDEINASA

Specific function: Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.6363636363636, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=33.6322869955157, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1787375, Length=217, Percent_Identity=29.9539170506912, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=31.2217194570136, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789809, Length=223, Percent_Identity=28.2511210762332, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786911, Length=221, Percent_Identity=32.1266968325792, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=30.1369863013699, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788394, Length=220, Percent_Identity=32.7272727272727, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=30.5309734513274, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367329, Length=224, Percent_Identity=33.0357142857143, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787229, Length=204, Percent_Identity=27.9411764705882, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790863, Length=225, Percent_Identity=23.1111111111111, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=128, Percent_Identity=32.03125, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25536; Mature: 25536

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQADMLISQQQFDAIILDLNLPDGDGI
CEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCH
EACERWKAQCFTSPIIMLTARSSLNERLAGFAVGADDYLIKPFAMEELVARLKVVAQRRP
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHEEECCCCCEECHHHHHHHHHHHHHHHHCCC
APQRLIIGDLEIDFANHMVYRQGQLLVLSKTGWQILALLARRSPETVSREEIERMLWPDS
CCCEEEEEEEEEEHHHHEEEECCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHCCCC
APDSDSLRSHIHLLRRVIDRPFERQILHTIRGVGLSLRDEINASA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MLVLLVEDNRLLSNNIIQYLELSGIECDYAFNLAQADMLISQQQFDAIILDLNLPDGDGI
CEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCH
EACERWKAQCFTSPIIMLTARSSLNERLAGFAVGADDYLIKPFAMEELVARLKVVAQRRP
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHEEECCCCCEECHHHHHHHHHHHHHHHHCCC
APQRLIIGDLEIDFANHMVYRQGQLLVLSKTGWQILALLARRSPETVSREEIERMLWPDS
CCCEEEEEEEEEEHHHHEEEECCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHCCCC
APDSDSLRSHIHLLRRVIDRPFERQILHTIRGVGLSLRDEINASA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]