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The map label for this gene is mnmE

Identifier: 119947214

GI number: 119947214

Start: 4426636

End: 4428003

Strand: Direct

Name: mnmE

Synonym: Ping_3612

Alternate gene names: 119947214

Gene position: 4426636-4428003 (Clockwise)

Preceding gene: 119947213

Following gene: 119947215

Centisome position: 97.08

GC content: 43.13

Gene sequence:

>1368_bases
ATGATCAAAGCTATTGATACAATTGTTGCTCAAGCAACCGCACCCGGTCGTGGTGGTGTTGGTATTATTCGCATTTCAGG
GCCAGATGTAGAAGCGGTCGCAAAAGTTATTTTAGGAAAAGTCCCTAAACTGCGTTTTGCCGAATATTTATCCTTTAGCG
ATCAGCATAATGAAGTGCTGGATCAAGGTATCGCCCTGTTTTTTAAAGCGCCCAATTCATTTACAGGTGAAGATGTACTG
GAACTCCAAGGGCATGGTGGTCCGGTGGTGATGGATATGCTTATTAAGGCAATTTTAAGCATAAAAAATCTTCGTGGAGC
CAATCCTGGAGAGTTTTCAGAACGTGCATTTATGAATGATAAACTCGATTTGGCACAAGCTGAAGGGATTGCTGATTTAA
TTGAAGCAACCTCAGAACAAGCCGCTAAAAGTGCGCTGCACTCTTTACAGGGTGAATTTTCAGAGAAAATAGAGCAGCTT
GTTGAAAGCCTTATCTATTTACGCATTTATGTTGAAGCCTCTATTGATTTCCCTGAAGAGGAAGTCGATTTCTTAAGTGA
CGGTAAAATTTCTAAAGGTTTATACCAAATAATTGATAATCTGGAAGCGGTAAAAAAGCAGGCCAAACAGGGCGCTATTT
TACGTGATGGCATGAAGGTTGTTATTGCAGGACGACCCAATGCCGGTAAATCAAGTTTATTAAACAGCCTGGTGGGTGTC
GAGCGCGCCATAGTGACCGACATTGCCGGTACCACCCGTGATGTGATGCGCGAACATATTCACATAGATGGTATGCCATT
ACATATTATTGATACTGCGGGTCTGCGTGAAGGTGCGGATGAAATTGAAAAAATAGGCATTGAGCGTGCCTGGCAGGAGA
TAACAACAGCGGATCGTATCCTTTTTATGCTTGATGCCACCACCACCAGTGCTGAAGATCCTCGTCAAATTTGGCCAGAT
TTTATTGATAAGCTGCCCAAATCTGTAGGTTTAACCGTCGTACGTAATAAAGCGGATCTGACTGGAGAGGCCTTCTCTAT
GACTGAAAACCATGATCACCCCGTTTATCGTATTTCAGCGAAAACCGGCCAGGGGGTGGACTTATTAAAAGAACATCTTA
AAGATATTATGGGTTATCAGGGCCATACCACCAGTGGTTTTATGGCAAGACGTCGCCATTTAGAGGCCATTGATAACGCA
CAGCGTCATTTGCTGGAGGGCAAAGTCCAACTGGAAGAGTATAAAGCGGGTGAATTATTAGCCGAAGAGTTAAGATTAAC
CCAACAATACCTGAGTGAAATTACCGGCGCATTCAGTTCCGATGATCTGCTTGGCAAAATTTTCTCAAGCTTCTGTATTG
GCAAATAA

Upstream 100 bases:

>100_bases
TTTATTTTCCCGATAACCATTGATAAAAAGGCGACTATAAAAAGTCGCCTTTTTTGTATAATAACTTTTTATATAATAAT
TGAAATTTTGAGGTCTAATC

Downstream 100 bases:

>100_bases
TCACTCTTTGCTGTTAATAAAAAGCCGCTACTCAGCGGTTTTTTTCAACCTAAAACAGTAATAGTTATCATTTCTATTAC
TGTTATTATTATAACCTGCT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MIKAIDTIVAQATAPGRGGVGIIRISGPDVEAVAKVILGKVPKLRFAEYLSFSDQHNEVLDQGIALFFKAPNSFTGEDVL
ELQGHGGPVVMDMLIKAILSIKNLRGANPGEFSERAFMNDKLDLAQAEGIADLIEATSEQAAKSALHSLQGEFSEKIEQL
VESLIYLRIYVEASIDFPEEEVDFLSDGKISKGLYQIIDNLEAVKKQAKQGAILRDGMKVVIAGRPNAGKSSLLNSLVGV
ERAIVTDIAGTTRDVMREHIHIDGMPLHIIDTAGLREGADEIEKIGIERAWQEITTADRILFMLDATTTSAEDPRQIWPD
FIDKLPKSVGLTVVRNKADLTGEAFSMTENHDHPVYRISAKTGQGVDLLKEHLKDIMGYQGHTTSGFMARRRHLEAIDNA
QRHLLEGKVQLEEYKAGELLAEELRLTQQYLSEITGAFSSDDLLGKIFSSFCIGK

Sequences:

>Translated_455_residues
MIKAIDTIVAQATAPGRGGVGIIRISGPDVEAVAKVILGKVPKLRFAEYLSFSDQHNEVLDQGIALFFKAPNSFTGEDVL
ELQGHGGPVVMDMLIKAILSIKNLRGANPGEFSERAFMNDKLDLAQAEGIADLIEATSEQAAKSALHSLQGEFSEKIEQL
VESLIYLRIYVEASIDFPEEEVDFLSDGKISKGLYQIIDNLEAVKKQAKQGAILRDGMKVVIAGRPNAGKSSLLNSLVGV
ERAIVTDIAGTTRDVMREHIHIDGMPLHIIDTAGLREGADEIEKIGIERAWQEITTADRILFMLDATTTSAEDPRQIWPD
FIDKLPKSVGLTVVRNKADLTGEAFSMTENHDHPVYRISAKTGQGVDLLKEHLKDIMGYQGHTTSGFMARRRHLEAIDNA
QRHLLEGKVQLEEYKAGELLAEELRLTQQYLSEITGAFSSDDLLGKIFSSFCIGK
>Mature_455_residues
MIKAIDTIVAQATAPGRGGVGIIRISGPDVEAVAKVILGKVPKLRFAEYLSFSDQHNEVLDQGIALFFKAPNSFTGEDVL
ELQGHGGPVVMDMLIKAILSIKNLRGANPGEFSERAFMNDKLDLAQAEGIADLIEATSEQAAKSALHSLQGEFSEKIEQL
VESLIYLRIYVEASIDFPEEEVDFLSDGKISKGLYQIIDNLEAVKKQAKQGAILRDGMKVVIAGRPNAGKSSLLNSLVGV
ERAIVTDIAGTTRDVMREHIHIDGMPLHIIDTAGLREGADEIEKIGIERAWQEITTADRILFMLDATTTSAEDPRQIWPD
FIDKLPKSVGLTVVRNKADLTGEAFSMTENHDHPVYRISAKTGQGVDLLKEHLKDIMGYQGHTTSGFMARRRHLEAIDNA
QRHLLEGKVQLEEYKAGELLAEELRLTQQYLSEITGAFSSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=471, Percent_Identity=36.3057324840764, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=34.1948310139165, Blast_Score=233, Evalue=4e-61,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=34.9240780911063, Blast_Score=228, Evalue=8e-60,
Organism=Escherichia coli, GI2367268, Length=450, Percent_Identity=69.3333333333333, Blast_Score=648, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=214, Percent_Identity=31.3084112149533, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=28.2608695652174, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=32.8571428571429, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=33.5416666666667, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PSYIN (A1T0N0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944894.1
- ProteinModelPortal:   A1T0N0
- SMR:   A1T0N0
- STRING:   A1T0N0
- GeneID:   4625126
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3612
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- PhylomeDB:   A1T0N0
- BioCyc:   PING357804:PING_3612-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49949; Mature: 49949

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 81-81 BINDING 121-121 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAIDTIVAQATAPGRGGVGIIRISGPDVEAVAKVILGKVPKLRFAEYLSFSDQHNEVL
CCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DQGIALFFKAPNSFTGEDVLELQGHGGPVVMDMLIKAILSIKNLRGANPGEFSERAFMND
HCCCEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
KLDLAQAEGIADLIEATSEQAAKSALHSLQGEFSEKIEQLVESLIYLRIYVEASIDFPEE
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHH
EVDFLSDGKISKGLYQIIDNLEAVKKQAKQGAILRDGMKVVIAGRPNAGKSSLLNSLVGV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHH
ERAIVTDIAGTTRDVMREHIHIDGMPLHIIDTAGLREGADEIEKIGIERAWQEITTADRI
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHE
LFMLDATTTSAEDPRQIWPDFIDKLPKSVGLTVVRNKADLTGEAFSMTENHDHPVYRISA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCCCEEEEEC
KTGQGVDLLKEHLKDIMGYQGHTTSGFMARRRHLEAIDNAQRHLLEGKVQLEEYKAGELL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AEELRLTQQYLSEITGAFSSDDLLGKIFSSFCIGK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKAIDTIVAQATAPGRGGVGIIRISGPDVEAVAKVILGKVPKLRFAEYLSFSDQHNEVL
CCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DQGIALFFKAPNSFTGEDVLELQGHGGPVVMDMLIKAILSIKNLRGANPGEFSERAFMND
HCCCEEEEECCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
KLDLAQAEGIADLIEATSEQAAKSALHSLQGEFSEKIEQLVESLIYLRIYVEASIDFPEE
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHH
EVDFLSDGKISKGLYQIIDNLEAVKKQAKQGAILRDGMKVVIAGRPNAGKSSLLNSLVGV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHHHHHHHH
ERAIVTDIAGTTRDVMREHIHIDGMPLHIIDTAGLREGADEIEKIGIERAWQEITTADRI
HHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHE
LFMLDATTTSAEDPRQIWPDFIDKLPKSVGLTVVRNKADLTGEAFSMTENHDHPVYRISA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCCCEEEEEC
KTGQGVDLLKEHLKDIMGYQGHTTSGFMARRRHLEAIDNAQRHLLEGKVQLEEYKAGELL
CCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AEELRLTQQYLSEITGAFSSDDLLGKIFSSFCIGK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA