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The map label for this gene is pdhB [H]

Identifier: 119947204

GI number: 119947204

Start: 4416559

End: 4417563

Strand: Direct

Name: pdhB [H]

Synonym: Ping_3602

Alternate gene names: 119947204

Gene position: 4416559-4417563 (Clockwise)

Preceding gene: 119947203

Following gene: 119947205

Centisome position: 96.86

GC content: 47.46

Gene sequence:

>1005_bases
ATGGATAAAATCACCTTCAGAGAGGCATTTAAGGAAGCGATAGCTGAAGCATTAAATAATGATGCCAGGGTCTTTTTAAT
CGGCGAAGATGTGGGTCGATATGGGGGTTGTTATGCGGTCTCCAAAGGATTGCTCGACAGGTTTGGCGCGCAACGCATTA
TTGATGCTCCTTTAGCGGAATCCGGCTTTGTCGGTGCAGGAATTGGTGCAGCAATAGGTGGAATGAGACCTATAGTCGAA
ATAATGACGGTCAATTTCAGCCTGCTCGCACTGGATCAAATCATCAATAATGCTGCGACGCTCAGGCATATGTCTGGCGG
GCAGATATCTGTACCCTTAGTCATTCGCATGTCCTGCGGGGCAGGTAAGCAACTAGCGGCTCAACATTCGCATAGTTTTG
AAGGTTTATATGCCCATATTCCCGGTCTTAAAGTACTTTATCCGGGCACTATCGGTGATGCGCGTTATATGCTGAAAATG
GCCCTTGATGATCCCGACCCGGTGATTATTTTTGAACATGTGATGCTCTATAATCAGGAGGCTGAACTACCGGAAAAACA
GACGATCGCGCCAATGGAAAAAGCGGTGATCCGCAAAACCGGTCATGATTTAAGCATTATCACCTGGGGAGGTTGTTTGT
ATAAAGCACTGGATGCAGCTGAGCAGCTTGCTGCCTTAGGCATTGATGCGGAAGTCGTCGATTTGCGCTGTTTACGCCCC
CTAGACAGAGCAACAATTTTAGATTCAGTGCGCCGTACACATAAGGCGCTGATTGTTGACGAATCATGGAAAAGCGGCGG
CATGTCCGCTGAAGTCAGCGCAACTATTGCAGAACTTGGACTCTGGTATTTAGATGCACCCGTTAATCGCGTTTGTAGTG
CTGAAGTACCTATTCCCTACGCATATCATTTAGAACAGGCGAGCTTGCCACAGGTAGCACAAATTATCGCAGTGGCGAAG
CAGATGATGGAACCATCCGATGCTGATACAAGGGATCTGTTATGA

Upstream 100 bases:

>100_bases
GCGGTGGAGAAAGAGATTGATGCGGCAGTGTGCAGCGCAGATGCGGCTGATATCGAGCCCGTCGAAAATTTGTTAAAGCA
TGTATATACAGAGGTGTCCA

Downstream 100 bases:

>100_bases
GCAACAATGAACATGACTTAAAGATGCCCTCTTTTGGTTCTGATATGAAAAAAGGAACCCTAGTACAGTGGCTGGTTAAA
GAGGGTGACCATATAAAACG

Product: pyruvate dehydrogenase complex, E1 beta2 component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MDKITFREAFKEAIAEALNNDARVFLIGEDVGRYGGCYAVSKGLLDRFGAQRIIDAPLAESGFVGAGIGAAIGGMRPIVE
IMTVNFSLLALDQIINNAATLRHMSGGQISVPLVIRMSCGAGKQLAAQHSHSFEGLYAHIPGLKVLYPGTIGDARYMLKM
ALDDPDPVIIFEHVMLYNQEAELPEKQTIAPMEKAVIRKTGHDLSIITWGGCLYKALDAAEQLAALGIDAEVVDLRCLRP
LDRATILDSVRRTHKALIVDESWKSGGMSAEVSATIAELGLWYLDAPVNRVCSAEVPIPYAYHLEQASLPQVAQIIAVAK
QMMEPSDADTRDLL

Sequences:

>Translated_334_residues
MDKITFREAFKEAIAEALNNDARVFLIGEDVGRYGGCYAVSKGLLDRFGAQRIIDAPLAESGFVGAGIGAAIGGMRPIVE
IMTVNFSLLALDQIINNAATLRHMSGGQISVPLVIRMSCGAGKQLAAQHSHSFEGLYAHIPGLKVLYPGTIGDARYMLKM
ALDDPDPVIIFEHVMLYNQEAELPEKQTIAPMEKAVIRKTGHDLSIITWGGCLYKALDAAEQLAALGIDAEVVDLRCLRP
LDRATILDSVRRTHKALIVDESWKSGGMSAEVSATIAELGLWYLDAPVNRVCSAEVPIPYAYHLEQASLPQVAQIIAVAK
QMMEPSDADTRDLL
>Mature_334_residues
MDKITFREAFKEAIAEALNNDARVFLIGEDVGRYGGCYAVSKGLLDRFGAQRIIDAPLAESGFVGAGIGAAIGGMRPIVE
IMTVNFSLLALDQIINNAATLRHMSGGQISVPLVIRMSCGAGKQLAAQHSHSFEGLYAHIPGLKVLYPGTIGDARYMLKM
ALDDPDPVIIFEHVMLYNQEAELPEKQTIAPMEKAVIRKTGHDLSIITWGGCLYKALDAAEQLAALGIDAEVVDLRCLRP
LDRATILDSVRRTHKALIVDESWKSGGMSAEVSATIAELGLWYLDAPVNRVCSAEVPIPYAYHLEQASLPQVAQIIAVAK
QMMEPSDADTRDLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=327, Percent_Identity=41.2844036697248, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=39.2638036809816, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4557353, Length=303, Percent_Identity=34.6534653465347, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI34101272, Length=303, Percent_Identity=34.6534653465347, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI205277463, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4507521, Length=305, Percent_Identity=25.5737704918033, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786622, Length=278, Percent_Identity=25.1798561151079, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=41.4110429447853, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=33.7461300309598, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=43.558282208589, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI21358145, Length=319, Percent_Identity=43.5736677115987, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24650940, Length=319, Percent_Identity=43.5736677115987, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI160714828, Length=303, Percent_Identity=34.983498349835, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI160714832, Length=303, Percent_Identity=34.983498349835, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=46.6666666666667, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=46.6666666666667, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36186; Mature: 36186

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKITFREAFKEAIAEALNNDARVFLIGEDVGRYGGCYAVSKGLLDRFGAQRIIDAPLAE
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCC
SGFVGAGIGAAIGGMRPIVEIMTVNFSLLALDQIINNAATLRHMSGGQISVPLVIRMSCG
CCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCHHHHEECCCCEEEEEEEEEECCC
AGKQLAAQHSHSFEGLYAHIPGLKVLYPGTIGDARYMLKMALDDPDPVIIFEHVMLYNQE
CCHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHEECCCCCCEEEEEHHHHHCCC
AELPEKQTIAPMEKAVIRKTGHDLSIITWGGCLYKALDAAEQLAALGIDAEVVDLRCLRP
CCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
LDRATILDSVRRTHKALIVDESWKSGGMSAEVSATIAELGLWYLDAPVNRVCSAEVPIPY
HHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
AYHLEQASLPQVAQIIAVAKQMMEPSDADTRDLL
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure
MDKITFREAFKEAIAEALNNDARVFLIGEDVGRYGGCYAVSKGLLDRFGAQRIIDAPLAE
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCC
SGFVGAGIGAAIGGMRPIVEIMTVNFSLLALDQIINNAATLRHMSGGQISVPLVIRMSCG
CCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCHHHHEECCCCEEEEEEEEEECCC
AGKQLAAQHSHSFEGLYAHIPGLKVLYPGTIGDARYMLKMALDDPDPVIIFEHVMLYNQE
CCHHHHHHHCCCCCCEEEECCCCEEEECCCCCCHHHHHHEECCCCCCEEEEEHHHHHCCC
AELPEKQTIAPMEKAVIRKTGHDLSIITWGGCLYKALDAAEQLAALGIDAEVVDLRCLRP
CCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
LDRATILDSVRRTHKALIVDESWKSGGMSAEVSATIAELGLWYLDAPVNRVCSAEVPIPY
HHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
AYHLEQASLPQVAQIIAVAKQMMEPSDADTRDLL
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]