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The map label for this gene is pdhC [H]

Identifier: 119947205

GI number: 119947205

Start: 4417560

End: 4418915

Strand: Direct

Name: pdhC [H]

Synonym: Ping_3603

Alternate gene names: 119947205

Gene position: 4417560-4418915 (Clockwise)

Preceding gene: 119947204

Following gene: 119947206

Centisome position: 96.88

GC content: 45.43

Gene sequence:

>1356_bases
ATGAGCAACAATGAACATGACTTAAAGATGCCCTCTTTTGGTTCTGATATGAAAAAAGGAACCCTAGTACAGTGGCTGGT
TAAAGAGGGTGACCATATAAAACGGGGTGATGTGGTTGCAGTTATTGAAACTCACAAAGGGGCAATAGATTTAGATCTAT
TTGAAGATGCATTAATAATCAGCTTACTGATCAAAGAAGGGCAGCAGATCGCGGTTGGAGAGCCTATTGCCAGATTAAGC
TCGACTAAAGACAGTGAAAATGCCCCCCTTCCTCAGACAGATGTTGCCGATATCGAGCCTAACCCTATATCCACAACAAG
CCCTGTAAATATAAGTGCAAGCCCTGCCTCCACAACAACTGCCACAGATATAAGTTCAAGCCCTGCGTCCAGTGCAGTTG
AGCAAGATAACAGTAATTTCACAACCCCTAAAGATTTTATTTTAGCAACACCCGCAGCGCGATTTTTGGCAACACAGCAG
CAACTCACTTTAAACTCACTGTTTCCCGAGCCTAGCAATAAAATTGTTACTTTGACTATGGTCGAGCAAGCAATAAAACA
AAAACAGCTCAGCCAGCAGTCACCCGCGATGACAGAAAATGTGCTGAAGCCTGCAGATAAAAAAGAAACCGCTAAGGTCA
AAGCCACCGATAGAGCAGCTGCAAGACCTAAAAAAGGTTTTGATAAAGAAGCGATGCGCCAAGCGATTAGCGCAACCGTG
ACACGCTCAAAACAACAAATACCACATTATTATTTACGCCAGCGTTTGGATATAACAGCGTTAGAGGACTACTTATTGCA
GGTTAACGCCAAGCTCGCCATCGATCAGCGCCTGCTCTTAGCAGCGCCTTTATTGTGTGCCGTTGCGCGTACATTAATGG
ATAGCCAGCAACTTAATGGCGAATATACAGAGGGCCACTTTGTCGCGAGTGAAGCAATACATTTAGCCAATGCGATTAAT
TTACGTGGCGGAGGATTAGTAATGCCAGTCATCCGCGCAGCACAAACTCTCTCCCCCTCGAAGATGATGGAACGTCTTAA
ACAGCAGGTGACACATGCGCGCAACGACAGTTTAGTCTTTTCTGAATTGAGTGGAGGCAGTTTTACTGTAACCAGCATTG
GTGAACGTGGTGCAGAGCAGATGTTCGCTGTTATTTTTCCACCACAGGTGGCGATTCTGGCTTTAGGTAGCCCTCATCAA
GAGGTCATGGCCGTCGATGGTTCGATAAAAATACGTTCAGTTATTGAGGCTTCTCTGGCGGCCGATCATCGTGTCAGTGA
TGGACGCATCGGAGCCCGTTTTCTTTATCAGTTAAACCAACTGCTGCAAAATCCGGAAGCTTTATGGACAGTTTGA

Upstream 100 bases:

>100_bases
CATATCATTTAGAACAGGCGAGCTTGCCACAGGTAGCACAAATTATCGCAGTGGCGAAGCAGATGATGGAACCATCCGAT
GCTGATACAAGGGATCTGTT

Downstream 100 bases:

>100_bases
ATGAGAGCGATACCTAGCCATGGTAAAGCAGATTAAAAATTGAAAGATAATAAAATGAGAGGACTTATGAACAATAATGA
AATAAAAGCACTGATTCTTG

Product: pyruvate dehydrogenase complex, E2 component dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSNNEHDLKMPSFGSDMKKGTLVQWLVKEGDHIKRGDVVAVIETHKGAIDLDLFEDALIISLLIKEGQQIAVGEPIARLS
STKDSENAPLPQTDVADIEPNPISTTSPVNISASPASTTTATDISSSPASSAVEQDNSNFTTPKDFILATPAARFLATQQ
QLTLNSLFPEPSNKIVTLTMVEQAIKQKQLSQQSPAMTENVLKPADKKETAKVKATDRAAARPKKGFDKEAMRQAISATV
TRSKQQIPHYYLRQRLDITALEDYLLQVNAKLAIDQRLLLAAPLLCAVARTLMDSQQLNGEYTEGHFVASEAIHLANAIN
LRGGGLVMPVIRAAQTLSPSKMMERLKQQVTHARNDSLVFSELSGGSFTVTSIGERGAEQMFAVIFPPQVAILALGSPHQ
EVMAVDGSIKIRSVIEASLAADHRVSDGRIGARFLYQLNQLLQNPEALWTV

Sequences:

>Translated_451_residues
MSNNEHDLKMPSFGSDMKKGTLVQWLVKEGDHIKRGDVVAVIETHKGAIDLDLFEDALIISLLIKEGQQIAVGEPIARLS
STKDSENAPLPQTDVADIEPNPISTTSPVNISASPASTTTATDISSSPASSAVEQDNSNFTTPKDFILATPAARFLATQQ
QLTLNSLFPEPSNKIVTLTMVEQAIKQKQLSQQSPAMTENVLKPADKKETAKVKATDRAAARPKKGFDKEAMRQAISATV
TRSKQQIPHYYLRQRLDITALEDYLLQVNAKLAIDQRLLLAAPLLCAVARTLMDSQQLNGEYTEGHFVASEAIHLANAIN
LRGGGLVMPVIRAAQTLSPSKMMERLKQQVTHARNDSLVFSELSGGSFTVTSIGERGAEQMFAVIFPPQVAILALGSPHQ
EVMAVDGSIKIRSVIEASLAADHRVSDGRIGARFLYQLNQLLQNPEALWTV
>Mature_450_residues
SNNEHDLKMPSFGSDMKKGTLVQWLVKEGDHIKRGDVVAVIETHKGAIDLDLFEDALIISLLIKEGQQIAVGEPIARLSS
TKDSENAPLPQTDVADIEPNPISTTSPVNISASPASTTTATDISSSPASSAVEQDNSNFTTPKDFILATPAARFLATQQQ
LTLNSLFPEPSNKIVTLTMVEQAIKQKQLSQQSPAMTENVLKPADKKETAKVKATDRAAARPKKGFDKEAMRQAISATVT
RSKQQIPHYYLRQRLDITALEDYLLQVNAKLAIDQRLLLAAPLLCAVARTLMDSQQLNGEYTEGHFVASEAIHLANAINL
RGGGLVMPVIRAAQTLSPSKMMERLKQQVTHARNDSLVFSELSGGSFTVTSIGERGAEQMFAVIFPPQVAILALGSPHQE
VMAVDGSIKIRSVIEASLAADHRVSDGRIGARFLYQLNQLLQNPEALWTV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=456, Percent_Identity=23.6842105263158, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI110671329, Length=468, Percent_Identity=24.1452991452991, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI203098816, Length=228, Percent_Identity=28.5087719298246, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI203098753, Length=228, Percent_Identity=28.5087719298246, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI19923748, Length=124, Percent_Identity=29.8387096774194, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI260898739, Length=127, Percent_Identity=32.2834645669291, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786946, Length=229, Percent_Identity=27.9475982532751, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786305, Length=217, Percent_Identity=29.9539170506912, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560088, Length=479, Percent_Identity=25.4697286012526, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25146366, Length=447, Percent_Identity=24.1610738255034, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17537937, Length=452, Percent_Identity=23.2300884955752, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=27.0358306188925, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=24.6724890829694, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=448, Percent_Identity=22.7678571428571, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582497, Length=218, Percent_Identity=27.9816513761468, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI20129315, Length=221, Percent_Identity=27.6018099547511, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24645909, Length=121, Percent_Identity=33.8842975206612, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18859875, Length=459, Percent_Identity=23.9651416122004, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48992; Mature: 48860

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNEHDLKMPSFGSDMKKGTLVQWLVKEGDHIKRGDVVAVIETHKGAIDLDLFEDALII
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHH
SLLIKEGQQIAVGEPIARLSSTKDSENAPLPQTDVADIEPNPISTTSPVNISASPASTTT
HHHHHCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
ATDISSSPASSAVEQDNSNFTTPKDFILATPAARFLATQQQLTLNSLFPEPSNKIVTLTM
CCCCCCCCCHHHHHCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEHHH
VEQAIKQKQLSQQSPAMTENVLKPADKKETAKVKATDRAAARPKKGFDKEAMRQAISATV
HHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHH
TRSKQQIPHYYLRQRLDITALEDYLLQVNAKLAIDQRLLLAAPLLCAVARTLMDSQQLNG
HHHHHHCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EYTEGHFVASEAIHLANAINLRGGGLVMPVIRAAQTLSPSKMMERLKQQVTHARNDSLVF
CCCCCEEHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
SELSGGSFTVTSIGERGAEQMFAVIFPPQVAILALGSPHQEVMAVDGSIKIRSVIEASLA
EECCCCEEEEEEHHHCCCCCEEEEEECCCEEEEEECCCCHHEEEECCCEEHHHHHHHHHH
ADHRVSDGRIGARFLYQLNQLLQNPEALWTV
HCCCCCCCCHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SNNEHDLKMPSFGSDMKKGTLVQWLVKEGDHIKRGDVVAVIETHKGAIDLDLFEDALII
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHH
SLLIKEGQQIAVGEPIARLSSTKDSENAPLPQTDVADIEPNPISTTSPVNISASPASTTT
HHHHHCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
ATDISSSPASSAVEQDNSNFTTPKDFILATPAARFLATQQQLTLNSLFPEPSNKIVTLTM
CCCCCCCCCHHHHHCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEHHH
VEQAIKQKQLSQQSPAMTENVLKPADKKETAKVKATDRAAARPKKGFDKEAMRQAISATV
HHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHH
TRSKQQIPHYYLRQRLDITALEDYLLQVNAKLAIDQRLLLAAPLLCAVARTLMDSQQLNG
HHHHHHCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EYTEGHFVASEAIHLANAINLRGGGLVMPVIRAAQTLSPSKMMERLKQQVTHARNDSLVF
CCCCCEEHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
SELSGGSFTVTSIGERGAEQMFAVIFPPQVAILALGSPHQEVMAVDGSIKIRSVIEASLA
EECCCCEEEEEEHHHCCCCCEEEEEECCCEEEEEECCCCHHEEEECCCEEHHHHHHHHHH
ADHRVSDGRIGARFLYQLNQLLQNPEALWTV
HCCCCCCCCHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]