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The map label for this gene is rlmA [H]

Identifier: 119946915

GI number: 119946915

Start: 4081311

End: 4082129

Strand: Direct

Name: rlmA [H]

Synonym: Ping_3309

Alternate gene names: 119946915

Gene position: 4081311-4082129 (Clockwise)

Preceding gene: 119946914

Following gene: 119946917

Centisome position: 89.51

GC content: 39.32

Gene sequence:

>819_bases
ATGACTCCTTTTATTTGTCCGCTTTGCCAAACTAAACTGCAACGCACTAATAACACCCAAATCTGCGCTAATAATCATCA
TTTTGATATTGCCAAGGAAGGTTATCTAAACCTGCTGCCGGTGAATGCTAAAAATTCAAAAAATCCGGGTGATAATAAAG
AGATGATCAGCGCACGCCGTGCTTTTTTAAACAGCGACGGCTATTTGCCATTAGCAGAAAAAGTCAGTGAGTTAGTGTCT
GCCTGCTTAGCAGAAAAAAAGCAGGCTCAAATTCTTGATTTAGGTTGCGGCGAAGGTTATTACACTGCACGTATAGCAGC
AAAGCTCCCGGAGCAGTTTATGATTAACGGCTTAGACATTTCCAAAGTAGCAATTCGTTATGCAGCTAAGCGCTATAAAA
ATATTAACTTCTGTGTTGCCAGCGCATACGATGTACCACTGGCGGATAATAGTTTAGATGCCTTAATCCGAATCTATGCA
CCGTCACTCGAAAGTGAATTACAACGATTAATCAAACCACAGGGTTATATAATAACCGTCACGCCTGCCCCGCGCCATCT
GTTTCAGTTACGTGAAAAAATATATGAACAGGTAAATCAACATGCCGAAGAAAATAAACCCGTGTCTGGTTTTAGCAAAA
TCAAGCAGATCAATCTAAATTACAGACTAACGATTAATAATCCTCAGACAGTGCAGGATTTAATTAATATGACACCCTTT
GCATGGAAATTTAACCCGCAAAAAATGCTCGCTTTATTAGCAGAAAAAACCTGGGAGATTGAATGTGACTTTAATATCGA
GGTTTATCAAAAAGAATGA

Upstream 100 bases:

>100_bases
GACAAAATAAAAGAGATTCTTTTACAATACCATCACTGCGTTATCTACTGAATAATAAACAGAGCAGGTAAATTTTTCAC
CTGCTCAAATAGGCCCCATT

Downstream 100 bases:

>100_bases
CAGCTGCGAGGGAGCAAGATCCGAGCCACGAGCTTCTAGCTGCGAACTTCGCTCGTAACTCGTGGCTCTCGACTCGAAGC
TCGAAGCTCGAAGCTCATAA

Product: rRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTPFICPLCQTKLQRTNNTQICANNHHFDIAKEGYLNLLPVNAKNSKNPGDNKEMISARRAFLNSDGYLPLAEKVSELVS
ACLAEKKQAQILDLGCGEGYYTARIAAKLPEQFMINGLDISKVAIRYAAKRYKNINFCVASAYDVPLADNSLDALIRIYA
PSLESELQRLIKPQGYIITVTPAPRHLFQLREKIYEQVNQHAEENKPVSGFSKIKQINLNYRLTINNPQTVQDLINMTPF
AWKFNPQKMLALLAEKTWEIECDFNIEVYQKE

Sequences:

>Translated_272_residues
MTPFICPLCQTKLQRTNNTQICANNHHFDIAKEGYLNLLPVNAKNSKNPGDNKEMISARRAFLNSDGYLPLAEKVSELVS
ACLAEKKQAQILDLGCGEGYYTARIAAKLPEQFMINGLDISKVAIRYAAKRYKNINFCVASAYDVPLADNSLDALIRIYA
PSLESELQRLIKPQGYIITVTPAPRHLFQLREKIYEQVNQHAEENKPVSGFSKIKQINLNYRLTINNPQTVQDLINMTPF
AWKFNPQKMLALLAEKTWEIECDFNIEVYQKE
>Mature_271_residues
TPFICPLCQTKLQRTNNTQICANNHHFDIAKEGYLNLLPVNAKNSKNPGDNKEMISARRAFLNSDGYLPLAEKVSELVSA
CLAEKKQAQILDLGCGEGYYTARIAAKLPEQFMINGLDISKVAIRYAAKRYKNINFCVASAYDVPLADNSLDALIRIYAP
SLESELQRLIKPQGYIITVTPAPRHLFQLREKIYEQVNQHAEENKPVSGFSKIKQINLNYRLTINNPQTVQDLINMTPFA
WKFNPQKMLALLAEKTWEIECDFNIEVYQKE

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=268, Percent_Identity=46.6417910447761, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 30959; Mature: 30828

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFICPLCQTKLQRTNNTQICANNHHFDIAKEGYLNLLPVNAKNSKNPGDNKEMISARR
CCCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHH
AFLNSDGYLPLAEKVSELVSACLAEKKQAQILDLGCGEGYYTARIAAKLPEQFMINGLDI
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHHCCHHHHHCCCCH
SKVAIRYAAKRYKNINFCVASAYDVPLADNSLDALIRIYAPSLESELQRLIKPQGYIITV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEE
TPAPRHLFQLREKIYEQVNQHAEENKPVSGFSKIKQINLNYRLTINNPQTVQDLINMTPF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCEEEEEECCCHHHHHHHCCCCC
AWKFNPQKMLALLAEKTWEIECDFNIEVYQKE
EEEECHHHHHHHHHCCCEEEEECCEEEEEECC
>Mature Secondary Structure 
TPFICPLCQTKLQRTNNTQICANNHHFDIAKEGYLNLLPVNAKNSKNPGDNKEMISARR
CCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHH
AFLNSDGYLPLAEKVSELVSACLAEKKQAQILDLGCGEGYYTARIAAKLPEQFMINGLDI
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHHCCHHHHHCCCCH
SKVAIRYAAKRYKNINFCVASAYDVPLADNSLDALIRIYAPSLESELQRLIKPQGYIITV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEE
TPAPRHLFQLREKIYEQVNQHAEENKPVSGFSKIKQINLNYRLTINNPQTVQDLINMTPF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCEEEEEECCCHHHHHHHCCCCC
AWKFNPQKMLALLAEKTWEIECDFNIEVYQKE
EEEECHHHHHHHHHCCCEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]