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The map label for this gene is pflA [H]

Identifier: 119946914

GI number: 119946914

Start: 4080285

End: 4081025

Strand: Direct

Name: pflA [H]

Synonym: Ping_3308

Alternate gene names: 119946914

Gene position: 4080285-4081025 (Clockwise)

Preceding gene: 119946913

Following gene: 119946915

Centisome position: 89.49

GC content: 42.65

Gene sequence:

>741_bases
ATGTCAGTAACAGGACGAGTTCATTCCGTTGAAAGTTGTGGTACCGTTGATGGTCCTGGGATCCGCTTTATTATTTTTTT
ACAGGGCTGTTTAATGCGCTGTCAATATTGTCATAACCGCGACACTTGGGATACCGAGGCCGGTAAAGAGATGAGCGTTG
AAGAATTAATGGCCGAACTGCTGCAATATCGCCATTACATGGAAGCATCGGGGGGCGGCATTACCGTTTCCGGGGGCGAA
GCAATGCTCCAGCCTGAATTTATTAAAGCGATCTTTGAAGCCTGCCATCTGGAGGGAATTCATACCTGTTTAGATACTAA
TGGTTTTGTGCGCCGAATCGATGACACGGTTAAAAGCGTGCTCGATCACACTGATCTAGTATTATTAGATATCAAACAGA
TGGACAATGATAAACATATCGACTTAACCCACGTCAGTAATAAATACACGCTGGAATTTGCTCACTATTTAGCAGAGCGT
CACCAAGCGGTCTATTTACGTTATGTGGTTTTACCCGGTTATACCGATGCTATCGATGATATACATGCCTTAGGTAAATT
TATAAAGCCTATGAAAAACATCGAAAAAATAGAGCTATTACCCTACCATGAATTAGGTAAGCATAAATGGACCGCAATGG
GGGAAATCTACCCGCTAGATGGCGTTAGCCCACCAACTCGTGAGAGTATGGACAAAATAAAAGAGATTCTTTTACAATAC
CATGACTGCGTTATCTACTGA

Upstream 100 bases:

>100_bases
TGTACGGATGAAACCTTTCTTTTTATCTATTCTAAAATGTTTATTTGCTTTGTTTTTTGCCTAAGATGCAAATCAAATAG
TCTATTTTAAGGGGTTTCTA

Downstream 100 bases:

>100_bases
ATAATAAACACAGCAGGTAAATTTTTCACCTGCTTGAACAATCGACACACCAATTCATGAGAGTATAGACAAAATAAAAG
AGATTCTTTTACAATACCAT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVTGRVHSVESCGTVDGPGIRFIIFLQGCLMRCQYCHNRDTWDTEAGKEMSVEELMAELLQYRHYMEASGGGITVSGGE
AMLQPEFIKAIFEACHLEGIHTCLDTNGFVRRIDDTVKSVLDHTDLVLLDIKQMDNDKHIDLTHVSNKYTLEFAHYLAER
HQAVYLRYVVLPGYTDAIDDIHALGKFIKPMKNIEKIELLPYHELGKHKWTAMGEIYPLDGVSPPTRESMDKIKEILLQY
HDCVIY

Sequences:

>Translated_246_residues
MSVTGRVHSVESCGTVDGPGIRFIIFLQGCLMRCQYCHNRDTWDTEAGKEMSVEELMAELLQYRHYMEASGGGITVSGGE
AMLQPEFIKAIFEACHLEGIHTCLDTNGFVRRIDDTVKSVLDHTDLVLLDIKQMDNDKHIDLTHVSNKYTLEFAHYLAER
HQAVYLRYVVLPGYTDAIDDIHALGKFIKPMKNIEKIELLPYHELGKHKWTAMGEIYPLDGVSPPTRESMDKIKEILLQY
HDCVIY
>Mature_245_residues
SVTGRVHSVESCGTVDGPGIRFIIFLQGCLMRCQYCHNRDTWDTEAGKEMSVEELMAELLQYRHYMEASGGGITVSGGEA
MLQPEFIKAIFEACHLEGIHTCLDTNGFVRRIDDTVKSVLDHTDLVLLDIKQMDNDKHIDLTHVSNKYTLEFAHYLAERH
QAVYLRYVVLPGYTDAIDDIHALGKFIKPMKNIEKIELLPYHELGKHKWTAMGEIYPLDGVSPPTRESMDKIKEILLQYH
DCVIY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=64.2276422764228, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1790389, Length=265, Percent_Identity=25.2830188679245, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790839, Length=240, Percent_Identity=28.75, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI226510931, Length=278, Percent_Identity=25.5395683453237, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28077; Mature: 27945

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTGRVHSVESCGTVDGPGIRFIIFLQGCLMRCQYCHNRDTWDTEAGKEMSVEELMAEL
CCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LQYRHYMEASGGGITVSGGEAMLQPEFIKAIFEACHLEGIHTCLDTNGFVRRIDDTVKSV
HHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LDHTDLVLLDIKQMDNDKHIDLTHVSNKYTLEFAHYLAERHQAVYLRYVVLPGYTDAIDD
HCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHH
IHALGKFIKPMKNIEKIELLPYHELGKHKWTAMGEIYPLDGVSPPTRESMDKIKEILLQY
HHHHHHHHHHHHCHHHEEECCCHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
HDCVIY
HHHHCC
>Mature Secondary Structure 
SVTGRVHSVESCGTVDGPGIRFIIFLQGCLMRCQYCHNRDTWDTEAGKEMSVEELMAEL
CCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LQYRHYMEASGGGITVSGGEAMLQPEFIKAIFEACHLEGIHTCLDTNGFVRRIDDTVKSV
HHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LDHTDLVLLDIKQMDNDKHIDLTHVSNKYTLEFAHYLAERHQAVYLRYVVLPGYTDAIDD
HCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHH
IHALGKFIKPMKNIEKIELLPYHELGKHKWTAMGEIYPLDGVSPPTRESMDKIKEILLQY
HHHHHHHHHHHHCHHHEEECCCHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
HDCVIY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]