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The map label for this gene is pflB [H]

Identifier: 119946913

GI number: 119946913

Start: 4077834

End: 4080119

Strand: Direct

Name: pflB [H]

Synonym: Ping_3307

Alternate gene names: 119946913

Gene position: 4077834-4080119 (Clockwise)

Preceding gene: 119946912

Following gene: 119946914

Centisome position: 89.43

GC content: 42.52

Gene sequence:

>2286_bases
ATGACTAAGATTAATGAAGAATTGGCATCAGCATGGCAAGGTTTTGCTGGTGAAGTTTGGAAAACTGACGTTAACGTACG
TGACTTCATCCAAAAAAACTATACCCCCTTTGAAGGTGATGAAAGTTTCCTGGCGGGTGCAACCCAAGCCACTGATGCGC
TTTGGGCTAAGGTGATGGAAGGCATCAAACTGGAAAACAGCACCCATGCGCCAGTTGATTTCGATACTGATATTGTTTCT
ACCATTACCGCTCATGACGCAGGTTATATCAACCAAGATTTAGAAACCATTGTTGGTCTGCAAACCGATGCGCCTTTAAA
ACGTGCGATTATCCCTAACGGCGGTATTCGTATGATCGATGGTTCTTGTAAAGTTTATGGTAAAGAGCTCGATCCAACTA
TTAATAAAATTTACTCTGAATTCCGTAAAACACATAATCAGGGTGTTTTTGATGTTTACACAGGTGACATCCTAAAATGT
CGTAAATCAGGTATCTTAACCGGTTTGCCCGATGCTTATGGTCGTGGTCGTATTATCGGTGATTACCGTCGTGTTGCCCT
GTACGGAATTGACTTCCTAATGACCGATAAAGCGGAGCAGCATAAAAGCTTAGAAAAAGATTTACTTGCCGGAGCAACAC
TTGAAGATACCCTGAAATTACGTGAAGAGATCTTTGAGCAATACCGTGCACTAGCACAAATTAAAGAGATGGCGGCTAAA
TACGGCTCGGATATCTCTCGTCCTGCACAAAATGCGAAAGAAGCCATTCAATGGACTTACTTTGGTTATCTGGCGGCAAT
CAAATCACAAAATGGCGCAGCAATGTCATTTGGTCGTACTATGAGTTTCCTTGATATCTATATTGAAAAAGATATGCAGG
CAGGTGTACTTACCGAGACTGAAGCACAAGAGCTTATCGATCACTTGGTCATGAAATTACGTATGGTACGTTTCCTACGT
ACACCTGAATACGATGATCTGTTCTCCGGCGACCCTATCTGGGCAACGGAGTCTATTGGCGGCATGGGCGTTGACGGGCG
TACGCTAGTGACTAAAAGCAGCTTCCGTATTCTGCATACACAATACACTATGGGCCCTTCTCCAGAGCCGAACATTACTG
TTTTATGGTCTGAAGCATTACCTGTTAACTTTAAAAAATACTGTGCAAAAGTATCGATTGATACTTCCTCTATTCAGTAT
GAAAATGATGACTTGATGCGCAGCAATTTTGATAACGATGATTACGCTATCGCCTGTTGTGTATCCCCTATGATTGTCGG
TAAGCAGATGCAGTTCTTTGGTGCGCGTGCCAACCTTGCTAAAACCTTGCTTTACGCCATTAACGGCGGCGTAGATGAAA
AATCTAAAGTGCAGGTTGGTCCTAGTACATTGCCTGCAATTACCTCTGAAGTCTTAGATTATGATGAAGTATTTGCGAAC
CTTGACCACTTTATGGAATGGTTAGCTGAAACCTACGTCACTGCACTTAACTGTATTCATTATATGCATGACAAATACAG
TTATGAAGCATCGCTAATGGCACTGCACGATCGTGATGTTGAACGTACTATGGCTTGTGGTATTGCCGGTCTTTCAATTG
CAGCTGATTCACTTTCTGCCATAAAATACGCAACCGTTAAACCTGTCCGTGATGAAGACGGTATTGCAACTGATTTTGAG
ATCGAAGGTGATTACCCTAAATTTGGTAACAATGACCCGCGTGTAGATGACATGGCTTGTGATCTTGTTGAACGTTTTAT
GAAAAAAATTCAATCGCACAAAATGTACCGCAATGCACGTGCGACTCAATCTATCCTTACTATTACTTCTAACGTGGTAT
ACGGTAAAAAAACCGGTAACACACCTGATGGACGTCGTTCTGGCATGCCCTTTGCGCCTGGTGCTAACCCCATGCATGGT
CGTGATGAAAAGGGTGCGGTTGCATCATTAACCTCTGTTTCTAAACTGCCATTTTCCTACGCACAGGATGGTATTTCATA
TACTTTCTCGATTGTACCAAATGCATTAGGTAAAACTGACGATTCACAAAAAACAAACTTAGCGGGTCTGATGGACGGTT
ACTTTAAGCACACCCCTGAGATTGAAGGTGGTCAGCACTTAAACGTAAACGTGATGAACCGTGAAATGCTGGTTGATGCA
ATGGATAATCCTGAAAAATACCCGCAGTTGACTATCCGTGTTTCTGGTTATGCAGTACGTTTTAACTCGTTAACTAAAGA
ACAGCAAAGCGATGTGATTTCACGTACCTTTACTGCCAGGTTGTAA

Upstream 100 bases:

>100_bases
GCAGAAAAGCAAACAAGAACAATAGAGTCTATTTAAACACGAACGATAGAGTCTATTTACAGTTCAACTCATACTTATTT
TAACTACAAGGTAAATCGTT

Downstream 100 bases:

>100_bases
CAAAAATATTCAGGCAACTCTTTTAGTTTGCCTGCTATAATTGCAAAGATTGCAAAGGTTTCATCTGTACGGATGAAACC
TTTCTTTTTATCTATTCTAA

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MTKINEELASAWQGFAGEVWKTDVNVRDFIQKNYTPFEGDESFLAGATQATDALWAKVMEGIKLENSTHAPVDFDTDIVS
TITAHDAGYINQDLETIVGLQTDAPLKRAIIPNGGIRMIDGSCKVYGKELDPTINKIYSEFRKTHNQGVFDVYTGDILKC
RKSGILTGLPDAYGRGRIIGDYRRVALYGIDFLMTDKAEQHKSLEKDLLAGATLEDTLKLREEIFEQYRALAQIKEMAAK
YGSDISRPAQNAKEAIQWTYFGYLAAIKSQNGAAMSFGRTMSFLDIYIEKDMQAGVLTETEAQELIDHLVMKLRMVRFLR
TPEYDDLFSGDPIWATESIGGMGVDGRTLVTKSSFRILHTQYTMGPSPEPNITVLWSEALPVNFKKYCAKVSIDTSSIQY
ENDDLMRSNFDNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGVDEKSKVQVGPSTLPAITSEVLDYDEVFAN
LDHFMEWLAETYVTALNCIHYMHDKYSYEASLMALHDRDVERTMACGIAGLSIAADSLSAIKYATVKPVRDEDGIATDFE
IEGDYPKFGNNDPRVDDMACDLVERFMKKIQSHKMYRNARATQSILTITSNVVYGKKTGNTPDGRRSGMPFAPGANPMHG
RDEKGAVASLTSVSKLPFSYAQDGISYTFSIVPNALGKTDDSQKTNLAGLMDGYFKHTPEIEGGQHLNVNVMNREMLVDA
MDNPEKYPQLTIRVSGYAVRFNSLTKEQQSDVISRTFTARL

Sequences:

>Translated_761_residues
MTKINEELASAWQGFAGEVWKTDVNVRDFIQKNYTPFEGDESFLAGATQATDALWAKVMEGIKLENSTHAPVDFDTDIVS
TITAHDAGYINQDLETIVGLQTDAPLKRAIIPNGGIRMIDGSCKVYGKELDPTINKIYSEFRKTHNQGVFDVYTGDILKC
RKSGILTGLPDAYGRGRIIGDYRRVALYGIDFLMTDKAEQHKSLEKDLLAGATLEDTLKLREEIFEQYRALAQIKEMAAK
YGSDISRPAQNAKEAIQWTYFGYLAAIKSQNGAAMSFGRTMSFLDIYIEKDMQAGVLTETEAQELIDHLVMKLRMVRFLR
TPEYDDLFSGDPIWATESIGGMGVDGRTLVTKSSFRILHTQYTMGPSPEPNITVLWSEALPVNFKKYCAKVSIDTSSIQY
ENDDLMRSNFDNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGVDEKSKVQVGPSTLPAITSEVLDYDEVFAN
LDHFMEWLAETYVTALNCIHYMHDKYSYEASLMALHDRDVERTMACGIAGLSIAADSLSAIKYATVKPVRDEDGIATDFE
IEGDYPKFGNNDPRVDDMACDLVERFMKKIQSHKMYRNARATQSILTITSNVVYGKKTGNTPDGRRSGMPFAPGANPMHG
RDEKGAVASLTSVSKLPFSYAQDGISYTFSIVPNALGKTDDSQKTNLAGLMDGYFKHTPEIEGGQHLNVNVMNREMLVDA
MDNPEKYPQLTIRVSGYAVRFNSLTKEQQSDVISRTFTARL
>Mature_760_residues
TKINEELASAWQGFAGEVWKTDVNVRDFIQKNYTPFEGDESFLAGATQATDALWAKVMEGIKLENSTHAPVDFDTDIVST
ITAHDAGYINQDLETIVGLQTDAPLKRAIIPNGGIRMIDGSCKVYGKELDPTINKIYSEFRKTHNQGVFDVYTGDILKCR
KSGILTGLPDAYGRGRIIGDYRRVALYGIDFLMTDKAEQHKSLEKDLLAGATLEDTLKLREEIFEQYRALAQIKEMAAKY
GSDISRPAQNAKEAIQWTYFGYLAAIKSQNGAAMSFGRTMSFLDIYIEKDMQAGVLTETEAQELIDHLVMKLRMVRFLRT
PEYDDLFSGDPIWATESIGGMGVDGRTLVTKSSFRILHTQYTMGPSPEPNITVLWSEALPVNFKKYCAKVSIDTSSIQYE
NDDLMRSNFDNDDYAIACCVSPMIVGKQMQFFGARANLAKTLLYAINGGVDEKSKVQVGPSTLPAITSEVLDYDEVFANL
DHFMEWLAETYVTALNCIHYMHDKYSYEASLMALHDRDVERTMACGIAGLSIAADSLSAIKYATVKPVRDEDGIATDFEI
EGDYPKFGNNDPRVDDMACDLVERFMKKIQSHKMYRNARATQSILTITSNVVYGKKTGNTPDGRRSGMPFAPGANPMHGR
DEKGAVASLTSVSKLPFSYAQDGISYTFSIVPNALGKTDDSQKTNLAGLMDGYFKHTPEIEGGQHLNVNVMNREMLVDAM
DNPEKYPQLTIRVSGYAVRFNSLTKEQQSDVISRTFTARL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=761, Percent_Identity=79.7634691195795, Blast_Score=1304, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=753, Percent_Identity=74.7675962815405, Blast_Score=1208, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=648, Percent_Identity=26.8518518518519, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1790388, Length=646, Percent_Identity=25.077399380805, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=78.6885245901639, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84755; Mature: 84624

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKINEELASAWQGFAGEVWKTDVNVRDFIQKNYTPFEGDESFLAGATQATDALWAKVME
CCCCHHHHHHHHCCCCCCCEECCCCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GIKLENSTHAPVDFDTDIVSTITAHDAGYINQDLETIVGLQTDAPLKRAIIPNGGIRMID
CCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEC
GSCKVYGKELDPTINKIYSEFRKTHNQGVFDVYTGDILKCRKSGILTGLPDAYGRGRIIG
CCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEH
DYRRVALYGIDFLMTDKAEQHKSLEKDLLAGATLEDTLKLREEIFEQYRALAQIKEMAAK
HHHEEHHHCCHHEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGSDISRPAQNAKEAIQWTYFGYLAAIKSQNGAAMSFGRTMSFLDIYIEKDMQAGVLTET
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCHH
EAQELIDHLVMKLRMVRFLRTPEYDDLFSGDPIWATESIGGMGVDGRTLVTKSSFRILHT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEE
QYTMGPSPEPNITVLWSEALPVNFKKYCAKVSIDTSSIQYENDDLMRSNFDNDDYAIACC
EEECCCCCCCCEEEEECCCCCCCHHHHHEEEEECCCCEEECCCHHHHCCCCCCCCEEEEE
VSPMIVGKQMQFFGARANLAKTLLYAINGGVDEKSKVQVGPSTLPAITSEVLDYDEVFAN
CCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
LDHFMEWLAETYVTALNCIHYMHDKYSYEASLMALHDRDVERTMACGIAGLSIAADSLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHH
IKYATVKPVRDEDGIATDFEIEGDYPKFGNNDPRVDDMACDLVERFMKKIQSHKMYRNAR
HEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQSILTITSNVVYGKKTGNTPDGRRSGMPFAPGANPMHGRDEKGAVASLTSVSKLPFSY
HHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHH
AQDGISYTFSIVPNALGKTDDSQKTNLAGLMDGYFKHTPEIEGGQHLNVNVMNREMLVDA
HHCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHC
MDNPEKYPQLTIRVSGYAVRFNSLTKEQQSDVISRTFTARL
CCCCCCCCEEEEEEECEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKINEELASAWQGFAGEVWKTDVNVRDFIQKNYTPFEGDESFLAGATQATDALWAKVME
CCCHHHHHHHHCCCCCCCEECCCCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GIKLENSTHAPVDFDTDIVSTITAHDAGYINQDLETIVGLQTDAPLKRAIIPNGGIRMID
CCEECCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEC
GSCKVYGKELDPTINKIYSEFRKTHNQGVFDVYTGDILKCRKSGILTGLPDAYGRGRIIG
CCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCEEH
DYRRVALYGIDFLMTDKAEQHKSLEKDLLAGATLEDTLKLREEIFEQYRALAQIKEMAAK
HHHEEHHHCCHHEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGSDISRPAQNAKEAIQWTYFGYLAAIKSQNGAAMSFGRTMSFLDIYIEKDMQAGVLTET
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCHH
EAQELIDHLVMKLRMVRFLRTPEYDDLFSGDPIWATESIGGMGVDGRTLVTKSSFRILHT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEE
QYTMGPSPEPNITVLWSEALPVNFKKYCAKVSIDTSSIQYENDDLMRSNFDNDDYAIACC
EEECCCCCCCCEEEEECCCCCCCHHHHHEEEEECCCCEEECCCHHHHCCCCCCCCEEEEE
VSPMIVGKQMQFFGARANLAKTLLYAINGGVDEKSKVQVGPSTLPAITSEVLDYDEVFAN
CCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
LDHFMEWLAETYVTALNCIHYMHDKYSYEASLMALHDRDVERTMACGIAGLSIAADSLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHH
IKYATVKPVRDEDGIATDFEIEGDYPKFGNNDPRVDDMACDLVERFMKKIQSHKMYRNAR
HEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ATQSILTITSNVVYGKKTGNTPDGRRSGMPFAPGANPMHGRDEKGAVASLTSVSKLPFSY
HHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHH
AQDGISYTFSIVPNALGKTDDSQKTNLAGLMDGYFKHTPEIEGGQHLNVNVMNREMLVDA
HHCCCEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHC
MDNPEKYPQLTIRVSGYAVRFNSLTKEQQSDVISRTFTARL
CCCCCCCCEEEEEEECEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]