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The map label for this gene is focA [H]

Identifier: 119946912

GI number: 119946912

Start: 4076889

End: 4077758

Strand: Direct

Name: focA [H]

Synonym: Ping_3306

Alternate gene names: 119946912

Gene position: 4076889-4077758 (Clockwise)

Preceding gene: 119946911

Following gene: 119946913

Centisome position: 89.41

GC content: 42.64

Gene sequence:

>870_bases
ATGCTTGCAGAAAATAAACGAGAAGAACTACTGCCTCATCAACTAACCGAAAAAGCCGCTGACGTTGCATATAATAAAGC
AACTAAAGCTCCCCTATCCGCTTTTTACCTCGCGATTACTGCAGGTCTTTTTATCGGCCTCGCTTTTGTTTTTTATGTCA
CCGTCACCACAGGCACTGAAAATGTCGCTTGGGGTCTTAGTAAATTTGCAGGTGGAGCAGCATTTAGTCTTGGTCTACTA
TTAATCGTTGTCGTGGGCGGTGAACTATTCACCAGCAGCACGCTGACCCTGATAGCGAAAGCGAGCAAACGTATCACGAC
AGCACAACTGCTTAAAAACTGGATCATTGTTTATTTTGGTAATTTTATTGGCGCAACCTTCCTTGTTTTCCTGATCTTTT
TTGCGGGAATGTACAATACAGATCACGGGCAATGGGGATTAACAGTCTTACATATTGCCCAGCATAAACTCCATCATTCA
TCTATAGAGGCCGTTTCACTGGGTATTTTATGTAATATGATGGTTTGTCTGGCAGTGTGGATGGCATTTGCCGCACGCAC
TATTACCGATAAAATGTTTGCCGTATTTCTACCTGTCACCATGTTTGTCGCCTCCGGTTTTGAGCATAGTATTGCCAATA
TGTTTATGGTGCCCAGCGGCATTTTAATCCAGCAATTTGCTCCCGCAGAATTTTGGCTCGCTATCGGAGAGCACACCAGC
CAATATTCTGATTTAACAATTTACAACTTTATTTTCGATAACCTAATTCCGGTGACCATCGGTAACATTATTGGCGGCGG
CGTAATAGTAGGTTTAATGCACTGGATGATCTATTTACGTCCAATGCAGAAAAGCAAACAAGAACAATAG

Upstream 100 bases:

>100_bases
ATAAAATTACAACCAATCCCTGGCAATTGATTTAAATCAAATTTATTACATAATCCATTTGTTAAACTTCTTGTACTCTC
AATAAACAGGAACGGACGAG

Downstream 100 bases:

>100_bases
AGTCTATTTAAACACGAACGATAGAGTCTATTTACAGTTCAACTCATACTTATTTTAACTACAAGGTAAATCGTTATGAC
TAAGATTAATGAAGAATTGG

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLAENKREELLPHQLTEKAADVAYNKATKAPLSAFYLAITAGLFIGLAFVFYVTVTTGTENVAWGLSKFAGGAAFSLGLL
LIVVVGGELFTSSTLTLIAKASKRITTAQLLKNWIIVYFGNFIGATFLVFLIFFAGMYNTDHGQWGLTVLHIAQHKLHHS
SIEAVSLGILCNMMVCLAVWMAFAARTITDKMFAVFLPVTMFVASGFEHSIANMFMVPSGILIQQFAPAEFWLAIGEHTS
QYSDLTIYNFIFDNLIPVTIGNIIGGGVIVGLMHWMIYLRPMQKSKQEQ

Sequences:

>Translated_289_residues
MLAENKREELLPHQLTEKAADVAYNKATKAPLSAFYLAITAGLFIGLAFVFYVTVTTGTENVAWGLSKFAGGAAFSLGLL
LIVVVGGELFTSSTLTLIAKASKRITTAQLLKNWIIVYFGNFIGATFLVFLIFFAGMYNTDHGQWGLTVLHIAQHKLHHS
SIEAVSLGILCNMMVCLAVWMAFAARTITDKMFAVFLPVTMFVASGFEHSIANMFMVPSGILIQQFAPAEFWLAIGEHTS
QYSDLTIYNFIFDNLIPVTIGNIIGGGVIVGLMHWMIYLRPMQKSKQEQ
>Mature_289_residues
MLAENKREELLPHQLTEKAADVAYNKATKAPLSAFYLAITAGLFIGLAFVFYVTVTTGTENVAWGLSKFAGGAAFSLGLL
LIVVVGGELFTSSTLTLIAKASKRITTAQLLKNWIIVYFGNFIGATFLVFLIFFAGMYNTDHGQWGLTVLHIAQHKLHHS
SIEAVSLGILCNMMVCLAVWMAFAARTITDKMFAVFLPVTMFVASGFEHSIANMFMVPSGILIQQFAPAEFWLAIGEHTS
QYSDLTIYNFIFDNLIPVTIGNIIGGGVIVGLMHWMIYLRPMQKSKQEQ

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=280, Percent_Identity=59.6428571428571, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1788837, Length=271, Percent_Identity=42.0664206642066, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI87082249, Length=274, Percent_Identity=29.9270072992701, Blast_Score=85, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321779, Length=296, Percent_Identity=24.6621621621622, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 31806; Mature: 31806

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]