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The map label for this gene is pyrI

Identifier: 119946901

GI number: 119946901

Start: 4067577

End: 4068032

Strand: Direct

Name: pyrI

Synonym: Ping_3295

Alternate gene names: 119946901

Gene position: 4067577-4068032 (Clockwise)

Preceding gene: 119946900

Following gene: 119946902

Centisome position: 89.21

GC content: 37.5

Gene sequence:

>456_bases
ATGCAAAAGAAATTACAAGTAGAAGCAATTGAGCACGGCTCCGTCATTGATCACATCCCGGTTCAACAGGGCGTCAAAAT
CATCCGATTTTTTAACTTAACACAAACCAATGAAAAAATTACCATCGGCCTGAACCTGGCAACCCACACCGGGCAAAAAA
AAGATCTGATAAAAGTCGAAAATACTTTTATCAGCGATAAACAAGCCAATCAGCTGGCCTTATTTGCACCCCATGCCACT
ATTAATCAGATAAAAGATTTTAAGGTGGTCAATAAATTTCAGGTGCAGCTACCAGACTCTTTTATTGATGTGTTAGCTTG
CCCGAACAGTAACTGCATCAGTCATCATGAGCCAGTAGATACACGTTTTTATGTCAAGAAAAAATCCACTCTCAAACTAA
AGTGTCATTATTGTGAAAAAACCTTTGATCATTTATTTTTCAGTGAATTAGACTAA

Upstream 100 bases:

>100_bases
TTATTATTTTCAACAAGCTCAAAATGGCATTTATGCACGACAGGCTTTATTGGCTTTATTGTTAACAAACCATTTCCAAG
ATCAAGCGTAAGGGCTAATT

Downstream 100 bases:

>100_bases
AGGATTGTCGCCCGACTTTTGTCCCAGGCCGATAATCAGGATCGACACTGTGATTTGGCCTTCGAGTTATTTGATTTTTA
TCACGCAAACTCAAAACTGT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MQKKLQVEAIEHGSVIDHIPVQQGVKIIRFFNLTQTNEKITIGLNLATHTGQKKDLIKVENTFISDKQANQLALFAPHAT
INQIKDFKVVNKFQVQLPDSFIDVLACPNSNCISHHEPVDTRFYVKKKSTLKLKCHYCEKTFDHLFFSELD

Sequences:

>Translated_151_residues
MQKKLQVEAIEHGSVIDHIPVQQGVKIIRFFNLTQTNEKITIGLNLATHTGQKKDLIKVENTFISDKQANQLALFAPHAT
INQIKDFKVVNKFQVQLPDSFIDVLACPNSNCISHHEPVDTRFYVKKKSTLKLKCHYCEKTFDHLFFSELD
>Mature_151_residues
MQKKLQVEAIEHGSVIDHIPVQQGVKIIRFFNLTQTNEKITIGLNLATHTGQKKDLIKVENTFISDKQANQLALFAPHAT
INQIKDFKVVNKFQVQLPDSFIDVLACPNSNCISHHEPVDTRFYVKKKSTLKLKCHYCEKTFDHLFFSELD

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=142, Percent_Identity=56.3380281690141, Blast_Score=174, Evalue=3e-45,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_PSYIN (A1SZR7)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944581.1
- ProteinModelPortal:   A1SZR7
- SMR:   A1SZR7
- STRING:   A1SZR7
- GeneID:   4625781
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3295
- eggNOG:   COG1781
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- PhylomeDB:   A1SZR7
- ProtClustDB:   PRK00893
- BioCyc:   PING357804:PING_3295-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17336; Mature: 17336

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKLQVEAIEHGSVIDHIPVQQGVKIIRFFNLTQTNEKITIGLNLATHTGQKKDLIKVE
CCCCEEEEEECCCCEECCCCHHHCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCEEEEE
NTFISDKQANQLALFAPHATINQIKDFKVVNKFQVQLPDSFIDVLACPNSNCISHHEPVD
CEEECCCCCCEEEEEECCCHHHHHHHEEEEEEEEEECCHHHEEEEECCCCCCCCCCCCCC
TRFYVKKKSTLKLKCHYCEKTFDHLFFSELD
CEEEEEECCEEEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKKLQVEAIEHGSVIDHIPVQQGVKIIRFFNLTQTNEKITIGLNLATHTGQKKDLIKVE
CCCCEEEEEECCCCEECCCCHHHCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCEEEEE
NTFISDKQANQLALFAPHATINQIKDFKVVNKFQVQLPDSFIDVLACPNSNCISHHEPVD
CEEECCCCCCEEEEEECCCHHHHHHHEEEEEEEEEECCHHHEEEEECCCCCCCCCCCCCC
TRFYVKKKSTLKLKCHYCEKTFDHLFFSELD
CEEEEEECCEEEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA