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The map label for this gene is pyrB

Identifier: 119946900

GI number: 119946900

Start: 4066632

End: 4067567

Strand: Direct

Name: pyrB

Synonym: Ping_3294

Alternate gene names: 119946900

Gene position: 4066632-4067567 (Clockwise)

Preceding gene: 119946898

Following gene: 119946901

Centisome position: 89.19

GC content: 38.68

Gene sequence:

>936_bases
ATGAATAATCCATTATATCAAAAAAATATTATCTCCATCTCCGACCTTTCTCGTGCAGAGCTTGAACTTATTCTAACAAC
AGCTCAATCATTAAAACACAACCCGCAACCCGAGCTGTTAAAAAATAAAGTTATTGCCAGTTGTTTCTTTGAAGCGTCAA
CACGAACCCGCCTTTCATTTGCAACAGCAGTGCAGAGGTTGGGAGGAACTGTGATTGGTTTTGATAATGCAGGAAATACC
TCATTAGCACAAAAAGGGGAAACACTGGCAGATTCAGTTAAAATAATCGCTTCTTATGCTGACGCTTTTTTTATACGCCA
CCCGCAGGAAGGTGCGGCTCGTTTAGCGGCGGAATTTACCAACATCCCTGTTATCAATGGTGGAGACGGATCGAATCAAC
ATCCTACGCAAACATTATTAGATCTGTTTACCATTTATGAAACGCAAAACAGACTCGATAACCTGAATATTGCCTTTGTA
GGCGATCTAAAATATGGCCGAACAGTGCACTCATTGGCACAAGCTTTGTCTTTATTTAACTGTAATTTCTATTTTATCGC
TCCAGAAGCTTTGGCAATGCCCGACTACATACTGGAAGAACTTAATGAGAAAGGGATCAATTATTCTGTGCATAGCAGTA
TTGAAGAGGTTGTTGATTCACTCGATGTGTTGTATATGACAAGGGTGCAAAAAGAACGTTTTGATGAAACGGAATATCAA
CATATTAAATCCGCATTTTTGTTAAATGCAGATATGTTAGAAAACGTGCGGGAAAACTTAAAAGTACTGCACCCGTTACC
TCGTGTGGATGAAATTAATATCAATGTCGATAAGACACCCTATGCTTATTATTTTCAACAAGCTCAAAATGGCATTTATG
CACGACAGGCTTTATTGGCTTTATTGTTAACAAACCATTTCCAAGATCAAGCGTAA

Upstream 100 bases:

>100_bases
AACATTTCACTTTGATTAAAGCATTTAAAAAAGGCAGAATTCATGGGGGCGCGGTGCTATAATTTCCCTATCGTCACTCG
TTACCCATGAGTCAGAAAAT

Downstream 100 bases:

>100_bases
GGGCTAATTATGCAAAAGAAATTACAAGTAGAAGCAATTGAGCACGGCTCCGTCATTGATCACATCCCGGTTCAACAGGG
CGTCAAAATCATCCGATTTT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNNPLYQKNIISISDLSRAELELILTTAQSLKHNPQPELLKNKVIASCFFEASTRTRLSFATAVQRLGGTVIGFDNAGNT
SLAQKGETLADSVKIIASYADAFFIRHPQEGAARLAAEFTNIPVINGGDGSNQHPTQTLLDLFTIYETQNRLDNLNIAFV
GDLKYGRTVHSLAQALSLFNCNFYFIAPEALAMPDYILEELNEKGINYSVHSSIEEVVDSLDVLYMTRVQKERFDETEYQ
HIKSAFLLNADMLENVRENLKVLHPLPRVDEININVDKTPYAYYFQQAQNGIYARQALLALLLTNHFQDQA

Sequences:

>Translated_311_residues
MNNPLYQKNIISISDLSRAELELILTTAQSLKHNPQPELLKNKVIASCFFEASTRTRLSFATAVQRLGGTVIGFDNAGNT
SLAQKGETLADSVKIIASYADAFFIRHPQEGAARLAAEFTNIPVINGGDGSNQHPTQTLLDLFTIYETQNRLDNLNIAFV
GDLKYGRTVHSLAQALSLFNCNFYFIAPEALAMPDYILEELNEKGINYSVHSSIEEVVDSLDVLYMTRVQKERFDETEYQ
HIKSAFLLNADMLENVRENLKVLHPLPRVDEININVDKTPYAYYFQQAQNGIYARQALLALLLTNHFQDQA
>Mature_311_residues
MNNPLYQKNIISISDLSRAELELILTTAQSLKHNPQPELLKNKVIASCFFEASTRTRLSFATAVQRLGGTVIGFDNAGNT
SLAQKGETLADSVKIIASYADAFFIRHPQEGAARLAAEFTNIPVINGGDGSNQHPTQTLLDLFTIYETQNRLDNLNIAFV
GDLKYGRTVHSLAQALSLFNCNFYFIAPEALAMPDYILEELNEKGINYSVHSSIEEVVDSLDVLYMTRVQKERFDETEYQ
HIKSAFLLNADMLENVRENLKVLHPLPRVDEININVDKTPYAYYFQQAQNGIYARQALLALLLTNHFQDQA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=45.3947368421053, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI38788445, Length=316, Percent_Identity=25, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI2367364, Length=305, Percent_Identity=70.4918032786885, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786469, Length=280, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790703, Length=286, Percent_Identity=26.5734265734266, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=40.8668730650155, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=40, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6322373, Length=334, Percent_Identity=24.5508982035928, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=44.9180327868852, Blast_Score=268, Evalue=2e-72,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PSYIN (A1SZR6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944580.1
- ProteinModelPortal:   A1SZR6
- SMR:   A1SZR6
- STRING:   A1SZR6
- GeneID:   4625780
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3294
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- PhylomeDB:   A1SZR6
- ProtClustDB:   PRK00856
- BioCyc:   PING357804:PING_3294-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34952; Mature: 34952

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPLYQKNIISISDLSRAELELILTTAQSLKHNPQPELLKNKVIASCFFEASTRTRLSF
CCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
ATAVQRLGGTVIGFDNAGNTSLAQKGETLADSVKIIASYADAFFIRHPQEGAARLAAEFT
HHHHHHHCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHH
NIPVINGGDGSNQHPTQTLLDLFTIYETQNRLDNLNIAFVGDLKYGRTVHSLAQALSLFN
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHC
CNFYFIAPEALAMPDYILEELNEKGINYSVHSSIEEVVDSLDVLYMTRVQKERFDETEYQ
CCEEEECCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HIKSAFLLNADMLENVRENLKVLHPLPRVDEININVDKTPYAYYFQQAQNGIYARQALLA
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHH
LLLTNHFQDQA
HHHHHHHCCCC
>Mature Secondary Structure
MNNPLYQKNIISISDLSRAELELILTTAQSLKHNPQPELLKNKVIASCFFEASTRTRLSF
CCCCCCCCCCEEHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
ATAVQRLGGTVIGFDNAGNTSLAQKGETLADSVKIIASYADAFFIRHPQEGAARLAAEFT
HHHHHHHCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHH
NIPVINGGDGSNQHPTQTLLDLFTIYETQNRLDNLNIAFVGDLKYGRTVHSLAQALSLFN
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHC
CNFYFIAPEALAMPDYILEELNEKGINYSVHSSIEEVVDSLDVLYMTRVQKERFDETEYQ
CCEEEECCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HIKSAFLLNADMLENVRENLKVLHPLPRVDEININVDKTPYAYYFQQAQNGIYARQALLA
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHH
LLLTNHFQDQA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA