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The map label for this gene is pykF [H]

Identifier: 119946502

GI number: 119946502

Start: 3559045

End: 3560454

Strand: Direct

Name: pykF [H]

Synonym: Ping_2879

Alternate gene names: 119946502

Gene position: 3559045-3560454 (Clockwise)

Preceding gene: 119946493

Following gene: 119946503

Centisome position: 78.06

GC content: 39.79

Gene sequence:

>1410_bases
GTGAGAAAAACTAAAATAGTTTGTACTATTGGTCCAAAATCAGAATCAAAAGAGATGTTAGGTAAATTAGTCAAAAATGG
CATGAATGTAATGCGCCTTAACTTCTCGCACGGTGATTTTAACGAACATGGTGGTCGAATTAATACTATCCGTGAAATTT
GCCGAGAAACGGGTAAAAATGTTGCCATTTTATTAGATACCAAAGGTCCTGAAATTCGTACAGTTAAACTAACCAACGGT
GCTGATGTATTATTAACGGCAGGACAAGAGTTCACTTTAACAACGGATCAAACTGTTGTGGGTGACAACACTGTGGTAGC
CGTAACTTATGAAAATCTAACCAATGATTTAGCGGTAGGTAACACGGTATTACTTGATGACGGCCTAATTGAACTAACCG
TTAAACGTATTACCGATACAGAGGTTATCTGTGATGTTATGAACACCGGTGAACTGGGCGAAAATAAAGGCGTTAACCTT
CCAGGTGTTAAAGTTCAGCTGCCTGCTCTGTCTCCTAAAGATAAAGGCGATTTAATATTTGGTTGTGAACAACAAGTTGA
TTTTATTGCAGCATCATTTATTCGTAAAAAAGAAGACGTATTGGAAATCCGTGAGTTATTAAAAGCTCACGGTGGCGAAA
AAATTCACATCATCTCTAAAATAGAAAACCAAGAAGGTGTTGATAACTTTGATGAAATCCTAGCAGCTTCAGACGCTATT
ATGGTCGCTCGTGGTGACTTAGGCGTTGAAATTGCAGTTGAAGAAGTGATCTTCGCACAAAAAATGATGATCGAAAAAGC
AAATGCTGCTCGAAAGCCTGTTATTACAGCGACACAAATGCTTGATTCAATGATCAAAAATCCACGACCAACGCGTGCTG
AAGCGGGCGATGTTGCCAATGCTATCTTAGATGGCACTGATGCAGTCATGCTTTCAGGTGAAAGTGCTAAAGGAAAATAC
CCTGCAGAAGCGGTTGAAATTATGGCAACTATCTGTGAGCGAACCGATCAAGTCATGCATGTCAGAGTTGATAATATTAC
AAAAGATATCCGTATCACAGAAGCAGTATGCAGCGGTGCAGTTAACAATGCAGAGCAACTAGATGCTAAATTAATCATAG
TAGCAACGGGTGCCGGCAAGTCTGCACGCTCTTTACGTAAATACTTTCCAAGGACTCAAATCCTGGCGTTAACCAGTAAT
CCAAAAACAGCACAACAACTTGCATTAGTAAAAGGGGTTTCTGCTAAAATTGTTGAAGCTCAAAATTCAATGGAAGAGTT
CTACCAACTTGGTATGGATATGAGTAAACAGCTTGGTCTTGTTACTGCTGGTGATAAAGTCATAATAGTCGCTGGTGCGC
TTGTGGCTTCCGGTACGACAAACACTTCTTCTGTACACGTTATTGTTTAA

Upstream 100 bases:

>100_bases
ATGCCCATCAAATACAGATATTGATAAACACGTTTTAATCTCTATTAAAAAAATTACAATTAATAAACTTAATAAACTTA
ATAACTATCGGAGAATAACG

Downstream 100 bases:

>100_bases
TCAATAAATAATCAGACTATTTAAGCATCACCTTATACCAATTCCACTAATTAAATGATCTATTTAGGCACCGGAAAAAC
GCATAAAAGCAAGGCATTGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRKTKIVCTIGPKSESKEMLGKLVKNGMNVMRLNFSHGDFNEHGGRINTIREICRETGKNVAILLDTKGPEIRTVKLTNG
ADVLLTAGQEFTLTTDQTVVGDNTVVAVTYENLTNDLAVGNTVLLDDGLIELTVKRITDTEVICDVMNTGELGENKGVNL
PGVKVQLPALSPKDKGDLIFGCEQQVDFIAASFIRKKEDVLEIRELLKAHGGEKIHIISKIENQEGVDNFDEILAASDAI
MVARGDLGVEIAVEEVIFAQKMMIEKANAARKPVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PAEAVEIMATICERTDQVMHVRVDNITKDIRITEAVCSGAVNNAEQLDAKLIIVATGAGKSARSLRKYFPRTQILALTSN
PKTAQQLALVKGVSAKIVEAQNSMEEFYQLGMDMSKQLGLVTAGDKVIIVAGALVASGTTNTSSVHVIV

Sequences:

>Translated_469_residues
MRKTKIVCTIGPKSESKEMLGKLVKNGMNVMRLNFSHGDFNEHGGRINTIREICRETGKNVAILLDTKGPEIRTVKLTNG
ADVLLTAGQEFTLTTDQTVVGDNTVVAVTYENLTNDLAVGNTVLLDDGLIELTVKRITDTEVICDVMNTGELGENKGVNL
PGVKVQLPALSPKDKGDLIFGCEQQVDFIAASFIRKKEDVLEIRELLKAHGGEKIHIISKIENQEGVDNFDEILAASDAI
MVARGDLGVEIAVEEVIFAQKMMIEKANAARKPVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PAEAVEIMATICERTDQVMHVRVDNITKDIRITEAVCSGAVNNAEQLDAKLIIVATGAGKSARSLRKYFPRTQILALTSN
PKTAQQLALVKGVSAKIVEAQNSMEEFYQLGMDMSKQLGLVTAGDKVIIVAGALVASGTTNTSSVHVIV
>Mature_469_residues
MRKTKIVCTIGPKSESKEMLGKLVKNGMNVMRLNFSHGDFNEHGGRINTIREICRETGKNVAILLDTKGPEIRTVKLTNG
ADVLLTAGQEFTLTTDQTVVGDNTVVAVTYENLTNDLAVGNTVLLDDGLIELTVKRITDTEVICDVMNTGELGENKGVNL
PGVKVQLPALSPKDKGDLIFGCEQQVDFIAASFIRKKEDVLEIRELLKAHGGEKIHIISKIENQEGVDNFDEILAASDAI
MVARGDLGVEIAVEEVIFAQKMMIEKANAARKPVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PAEAVEIMATICERTDQVMHVRVDNITKDIRITEAVCSGAVNNAEQLDAKLIIVATGAGKSARSLRKYFPRTQILALTSN
PKTAQQLALVKGVSAKIVEAQNSMEEFYQLGMDMSKQLGLVTAGDKVIIVAGALVASGTTNTSSVHVIV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=46.530612244898, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=45.9016393442623, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=45.9016393442623, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI32967597, Length=488, Percent_Identity=44.4672131147541, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI10835121, Length=488, Percent_Identity=44.4672131147541, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=50, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=50, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=46.1864406779661, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=46.1864406779661, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=45.1162790697674, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=71.063829787234, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=37.7637130801688, Blast_Score=287, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=476, Percent_Identity=44.5378151260504, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71984406, Length=480, Percent_Identity=45.625, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71984413, Length=480, Percent_Identity=45.625, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17506831, Length=480, Percent_Identity=45.625, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17506829, Length=480, Percent_Identity=45.625, Blast_Score=358, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=42.3553719008264, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6324923, Length=480, Percent_Identity=42.5, Blast_Score=348, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=45.8847736625514, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=45.8847736625514, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24648966, Length=456, Percent_Identity=42.7631578947368, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24581235, Length=484, Percent_Identity=34.297520661157, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24646914, Length=316, Percent_Identity=40.5063291139241, Blast_Score=205, Evalue=6e-53,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50486; Mature: 50486

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIVCTIGPKSESKEMLGKLVKNGMNVMRLNFSHGDFNEHGGRINTIREICRETGKN
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHCCCEEHHHHHHHHHCCCC
VAILLDTKGPEIRTVKLTNGADVLLTAGQEFTLTTDQTVVGDNTVVAVTYENLTNDLAVG
EEEEEECCCCCEEEEEECCCCEEEEECCCEEEEECCCEEECCCEEEEEEECCCCCCHHCC
NTVLLDDGLIELTVKRITDTEVICDVMNTGELGENKGVNLPGVKVQLPALSPKDKGDLIF
CEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEE
GCEQQVDFIAASFIRKKEDVLEIRELLKAHGGEKIHIISKIENQEGVDNFDEILAASDAI
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCEE
MVARGDLGVEIAVEEVIFAQKMMIEKANAARKPVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPAEAVEIMATICERTDQVMHVRVDNITKDIRITEAVCSGA
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCC
VNNAEQLDAKLIIVATGAGKSARSLRKYFPRTQILALTSNPKTAQQLALVKGVSAKIVEA
CCCHHHCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHH
QNSMEEFYQLGMDMSKQLGLVTAGDKVIIVAGALVASGTTNTSSVHVIV
HHHHHHHHHHCCCHHHHCCEEECCCEEEEEEEEEEECCCCCCCEEEEEC
>Mature Secondary Structure
MRKTKIVCTIGPKSESKEMLGKLVKNGMNVMRLNFSHGDFNEHGGRINTIREICRETGKN
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHCCCEEHHHHHHHHHCCCC
VAILLDTKGPEIRTVKLTNGADVLLTAGQEFTLTTDQTVVGDNTVVAVTYENLTNDLAVG
EEEEEECCCCCEEEEEECCCCEEEEECCCEEEEECCCEEECCCEEEEEEECCCCCCHHCC
NTVLLDDGLIELTVKRITDTEVICDVMNTGELGENKGVNLPGVKVQLPALSPKDKGDLIF
CEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEE
GCEQQVDFIAASFIRKKEDVLEIRELLKAHGGEKIHIISKIENQEGVDNFDEILAASDAI
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCEE
MVARGDLGVEIAVEEVIFAQKMMIEKANAARKPVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPAEAVEIMATICERTDQVMHVRVDNITKDIRITEAVCSGA
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCC
VNNAEQLDAKLIIVATGAGKSARSLRKYFPRTQILALTSNPKTAQQLALVKGVSAKIVEA
CCCHHHCCEEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHH
QNSMEEFYQLGMDMSKQLGLVTAGDKVIIVAGALVASGTTNTSSVHVIV
HHHHHHHHHHCCCHHHHCCEEECCCEEEEEEEEEEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]