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The map label for this gene is priA [H]

Identifier: 119946493

GI number: 119946493

Start: 3549101

End: 3551362

Strand: Direct

Name: priA [H]

Synonym: Ping_2869

Alternate gene names: 119946493

Gene position: 3549101-3551362 (Clockwise)

Preceding gene: 119946492

Following gene: 119946502

Centisome position: 77.84

GC content: 42.48

Gene sequence:

>2262_bases
ATGAGTTTTTATCTTCATTACATTGAGTGTATGACCCAACCTATCATTTGCCGCATCGCATTAGCGATTCCTCTGCATAA
ACAATTTGATTATCTGCTGCCTGATTATCTGTTAAAAACAGAGCCCAACAACCTGGTAGGCTGCCGAGTACGTGTGCCCT
TTGGTGGCAAACGACAACTTATTGGGGTGATCTGCGAAGTGAATCCAAAATGTGATTACGCCATCGATAAACTAAAAAAA
ATCACCCAATTGATTGATCAGACCCCTTTATTGGATAAAAACATCTGGTCACTGCTGCGTTGGGCGGCTTTTTATTACCA
GTATGCTTTTGGTGATGTCTTTCAGCAGGCATTACCGTCTTCACTACGCCAGGGTAAAGAAGCCATTTTTAAGGGTTATG
AACATTGGCAATTAACCGCCAATCCTTTTAATTTGAATGATTTTAAACGTGCCCAAAAGCAGCAGTCTGCCTTACAAATT
TTAAGCGAATCAAGTTTATCCAATCAGCAGTTAAGCGCACAGGGGATAAGTCGCCCAACGCTCAAAGCATTACAGGAAAA
AGGGGTTATTGAGTCCGTTGATAAACAACCGGTCAGCAATCTCAATTGGTTAGCTCAATTTGAAGAATCGGCACAAAAAC
CGGCTTTAACCGTCGAACAAGCTATTGCTATAAGCTGCGTTAATCAGCAGTTAGAGGCTTTTAACTGCTTTTTATTAGAG
GGCATAACAGGCAGTGGAAAAACCGAAGTTTACCTTAATATTATTAAACCAGTCTTACTCGCGGGTAAACAGGCATTGGT
AATAGTACCTGAAATCGGTTTAACGCCGCAAACCATTAAACGTTTTCAGGCACGTTTTAATGTCCCTGTTTATCTTAAAC
ATTCAGGATTAAACCCAAGAGAGCAACTCGATACCTGGCTGCATGCAAAACAAGGCAGCGGCGCCATTATTATCGGTACA
CGCAGCAGCATATTTTGCGATTTTAAAAATTTAGGCTTAATCATTTTAGATGAAGAGCATGATGCCTCTTTTAAACAACA
GGATGGTTTTCGTTATCATGCGCGTGATTTTGCAATCAAACGTGCCGCCTTGCAGAAAATCCCCATTATTTTAGGCTCTG
CCACGCCGTCCTTAGAAACATTAAACAATGCCCTGCAGGGAAAATATCAGCACTTGCATCTGACCGAGCGCCCGGGTAAT
GCTAAACCACCCACCAACAGCCTGATTAATTTAGCCGGTTTACCGCTAAAATCAGGACTATCTCCGCAGTTAATTGAATT
AATGAAAAAACACCTGGATAAAAATAATCAGGTTATTCTTTTTTTAAACCGCCGTGGTTATGCCCCTGTCTTAATGTGCC
ATGAATGCGGGTGGTTAGTTAAATGTGGCCGTTGTGATGCCTTTTACACTTACCATAAATCAGCTAATTACCTGCACTGC
CACCACTGCGCTTCCACTCTGCCCGTTCCTCATCAGTGTGATGATTGTGGCTCGACACAATTAAACTCAACAGGAGTGGG
TACAGAGCAGTTAGAGGAGACCTTAAACGACCTTTTCCCGGATCATCCGACGGTCCGTATTGACCGTGATAATACCCGCA
AAAAAGAGAGCTTTAATCAATATTTAACCGATATCAACAGCGGTAAATATAAAATTTTACTCGGCACCCAGATGCTGGCA
AAGGGCCATCACTTCCCCGATGTCACGCTGGTCGCATTAATAGATGTTGATGGCGCACTGTTTTGTAATGACTATCGTGC
CAGCGAAAGATTAGCACAGCTGTTTACGCAAGTATCAGGTCGCGCAGGACGAGCAGAAAAAAAGGGTCAAGTGGTACTGC
AAACACATCACCCTGAGCACAGTTTACTTCAGGATCTTATCAATAACGGTTATTTTGAATTTTCCCGTAATGCGCTGCAG
GAGCGAAAGCTTGCGCAGTTACCGCCAAGTACATTTCAAGCTTTATTAAGAGCGGAGTCCGATAATGCAACGCTGGCTGA
AAACTTCCTGTCTTTATGTCAGCAAATCTTGCAACAAATCTGCATTAACAATAACAGCCTTGATAAGTTATTAATTTTAG
GACCGATTCCTGCATCAATGGAAAGACGCGCAGGAAAGTATCGTTTTCAACTCTTATTACAAAGTGAACAACGCGCCGTG
ATCACGCAGATACTACATCGGGCATTACACGCTTTTGATAACCTGCCGGAAGGGCGAAAAGTAAGATGGTCTATTGATGT
TGATCCGATTGATTTTGTTTAA

Upstream 100 bases:

>100_bases
ATATTAGAAGCGTACTCTCCCGGCACTTCCTGTTGCTGTTTATAAACAGGTTACATTTCCATGGGCGTTTTTTTACCCGC
TTTATGTGAAACAGGTTACA

Downstream 100 bases:

>100_bases
GTTGAGAACGAGTTGAGGGCTGCGAGCTGATATCTTAAAGCTTAAAGCCGAAAGCTACAGGCAATAAAAAACCGAGATAA
TTCTCGGTTTTTTAAATCAG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MSFYLHYIECMTQPIICRIALAIPLHKQFDYLLPDYLLKTEPNNLVGCRVRVPFGGKRQLIGVICEVNPKCDYAIDKLKK
ITQLIDQTPLLDKNIWSLLRWAAFYYQYAFGDVFQQALPSSLRQGKEAIFKGYEHWQLTANPFNLNDFKRAQKQQSALQI
LSESSLSNQQLSAQGISRPTLKALQEKGVIESVDKQPVSNLNWLAQFEESAQKPALTVEQAIAISCVNQQLEAFNCFLLE
GITGSGKTEVYLNIIKPVLLAGKQALVIVPEIGLTPQTIKRFQARFNVPVYLKHSGLNPREQLDTWLHAKQGSGAIIIGT
RSSIFCDFKNLGLIILDEEHDASFKQQDGFRYHARDFAIKRAALQKIPIILGSATPSLETLNNALQGKYQHLHLTERPGN
AKPPTNSLINLAGLPLKSGLSPQLIELMKKHLDKNNQVILFLNRRGYAPVLMCHECGWLVKCGRCDAFYTYHKSANYLHC
HHCASTLPVPHQCDDCGSTQLNSTGVGTEQLEETLNDLFPDHPTVRIDRDNTRKKESFNQYLTDINSGKYKILLGTQMLA
KGHHFPDVTLVALIDVDGALFCNDYRASERLAQLFTQVSGRAGRAEKKGQVVLQTHHPEHSLLQDLINNGYFEFSRNALQ
ERKLAQLPPSTFQALLRAESDNATLAENFLSLCQQILQQICINNNSLDKLLILGPIPASMERRAGKYRFQLLLQSEQRAV
ITQILHRALHAFDNLPEGRKVRWSIDVDPIDFV

Sequences:

>Translated_753_residues
MSFYLHYIECMTQPIICRIALAIPLHKQFDYLLPDYLLKTEPNNLVGCRVRVPFGGKRQLIGVICEVNPKCDYAIDKLKK
ITQLIDQTPLLDKNIWSLLRWAAFYYQYAFGDVFQQALPSSLRQGKEAIFKGYEHWQLTANPFNLNDFKRAQKQQSALQI
LSESSLSNQQLSAQGISRPTLKALQEKGVIESVDKQPVSNLNWLAQFEESAQKPALTVEQAIAISCVNQQLEAFNCFLLE
GITGSGKTEVYLNIIKPVLLAGKQALVIVPEIGLTPQTIKRFQARFNVPVYLKHSGLNPREQLDTWLHAKQGSGAIIIGT
RSSIFCDFKNLGLIILDEEHDASFKQQDGFRYHARDFAIKRAALQKIPIILGSATPSLETLNNALQGKYQHLHLTERPGN
AKPPTNSLINLAGLPLKSGLSPQLIELMKKHLDKNNQVILFLNRRGYAPVLMCHECGWLVKCGRCDAFYTYHKSANYLHC
HHCASTLPVPHQCDDCGSTQLNSTGVGTEQLEETLNDLFPDHPTVRIDRDNTRKKESFNQYLTDINSGKYKILLGTQMLA
KGHHFPDVTLVALIDVDGALFCNDYRASERLAQLFTQVSGRAGRAEKKGQVVLQTHHPEHSLLQDLINNGYFEFSRNALQ
ERKLAQLPPSTFQALLRAESDNATLAENFLSLCQQILQQICINNNSLDKLLILGPIPASMERRAGKYRFQLLLQSEQRAV
ITQILHRALHAFDNLPEGRKVRWSIDVDPIDFV
>Mature_752_residues
SFYLHYIECMTQPIICRIALAIPLHKQFDYLLPDYLLKTEPNNLVGCRVRVPFGGKRQLIGVICEVNPKCDYAIDKLKKI
TQLIDQTPLLDKNIWSLLRWAAFYYQYAFGDVFQQALPSSLRQGKEAIFKGYEHWQLTANPFNLNDFKRAQKQQSALQIL
SESSLSNQQLSAQGISRPTLKALQEKGVIESVDKQPVSNLNWLAQFEESAQKPALTVEQAIAISCVNQQLEAFNCFLLEG
ITGSGKTEVYLNIIKPVLLAGKQALVIVPEIGLTPQTIKRFQARFNVPVYLKHSGLNPREQLDTWLHAKQGSGAIIIGTR
SSIFCDFKNLGLIILDEEHDASFKQQDGFRYHARDFAIKRAALQKIPIILGSATPSLETLNNALQGKYQHLHLTERPGNA
KPPTNSLINLAGLPLKSGLSPQLIELMKKHLDKNNQVILFLNRRGYAPVLMCHECGWLVKCGRCDAFYTYHKSANYLHCH
HCASTLPVPHQCDDCGSTQLNSTGVGTEQLEETLNDLFPDHPTVRIDRDNTRKKESFNQYLTDINSGKYKILLGTQMLAK
GHHFPDVTLVALIDVDGALFCNDYRASERLAQLFTQVSGRAGRAEKKGQVVLQTHHPEHSLLQDLINNGYFEFSRNALQE
RKLAQLPPSTFQALLRAESDNATLAENFLSLCQQILQQICINNNSLDKLLILGPIPASMERRAGKYRFQLLLQSEQRAVI
TQILHRALHAFDNLPEGRKVRWSIDVDPIDFV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=738, Percent_Identity=49.5934959349593, Blast_Score=757, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84967; Mature: 84836

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYLHYIECMTQPIICRIALAIPLHKQFDYLLPDYLLKTEPNNLVGCRVRVPFGGKRQL
CCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCCCEE
IGVICEVNPKCDYAIDKLKKITQLIDQTPLLDKNIWSLLRWAAFYYQYAFGDVFQQALPS
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLRQGKEAIFKGYEHWQLTANPFNLNDFKRAQKQQSALQILSESSLSNQQLSAQGISRPT
HHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHH
LKALQEKGVIESVDKQPVSNLNWLAQFEESAQKPALTVEQAIAISCVNQQLEAFNCFLLE
HHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEE
GITGSGKTEVYLNIIKPVLLAGKQALVIVPEIGLTPQTIKRFQARFNVPVYLKHSGLNPR
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHH
EQLDTWLHAKQGSGAIIIGTRSSIFCDFKNLGLIILDEEHDASFKQQDGFRYHARDFAIK
HHHHHHHHCCCCCCEEEEECCCCEEEECCCCCEEEEECCCCCCCHHCCCCEEHHHHHHHH
RAALQKIPIILGSATPSLETLNNALQGKYQHLHLTERPGNAKPPTNSLINLAGLPLKSGL
HHHHHHCCEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCC
SPQLIELMKKHLDKNNQVILFLNRRGYAPVLMCHECGWLVKCGRCDAFYTYHKSANYLHC
CHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCEEECCCCCEEEEECCCCCEEEH
HHCASTLPVPHQCDDCGSTQLNSTGVGTEQLEETLNDLFPDHPTVRIDRDNTRKKESFNQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
YLTDINSGKYKILLGTQMLAKGHHFPDVTLVALIDVDGALFCNDYRASERLAQLFTQVSG
HHHHCCCCCEEEEECHHHHHCCCCCCCEEEEEEEECCCEEEECCHHHHHHHHHHHHHHCC
RAGRAEKKGQVVLQTHHPEHSLLQDLINNGYFEFSRNALQERKLAQLPPSTFQALLRAES
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
DNATLAENFLSLCQQILQQICINNNSLDKLLILGPIPASMERRAGKYRFQLLLQSEQRAV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCHHHH
ITQILHRALHAFDNLPEGRKVRWSIDVDPIDFV
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SFYLHYIECMTQPIICRIALAIPLHKQFDYLLPDYLLKTEPNNLVGCRVRVPFGGKRQL
CHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCCCEE
IGVICEVNPKCDYAIDKLKKITQLIDQTPLLDKNIWSLLRWAAFYYQYAFGDVFQQALPS
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLRQGKEAIFKGYEHWQLTANPFNLNDFKRAQKQQSALQILSESSLSNQQLSAQGISRPT
HHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHH
LKALQEKGVIESVDKQPVSNLNWLAQFEESAQKPALTVEQAIAISCVNQQLEAFNCFLLE
HHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEE
GITGSGKTEVYLNIIKPVLLAGKQALVIVPEIGLTPQTIKRFQARFNVPVYLKHSGLNPR
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHH
EQLDTWLHAKQGSGAIIIGTRSSIFCDFKNLGLIILDEEHDASFKQQDGFRYHARDFAIK
HHHHHHHHCCCCCCEEEEECCCCEEEECCCCCEEEEECCCCCCCHHCCCCEEHHHHHHHH
RAALQKIPIILGSATPSLETLNNALQGKYQHLHLTERPGNAKPPTNSLINLAGLPLKSGL
HHHHHHCCEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCC
SPQLIELMKKHLDKNNQVILFLNRRGYAPVLMCHECGWLVKCGRCDAFYTYHKSANYLHC
CHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECCCCEEECCCCCEEEEECCCCCEEEH
HHCASTLPVPHQCDDCGSTQLNSTGVGTEQLEETLNDLFPDHPTVRIDRDNTRKKESFNQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
YLTDINSGKYKILLGTQMLAKGHHFPDVTLVALIDVDGALFCNDYRASERLAQLFTQVSG
HHHHCCCCCEEEEECHHHHHCCCCCCCEEEEEEEECCCEEEECCHHHHHHHHHHHHHHCC
RAGRAEKKGQVVLQTHHPEHSLLQDLINNGYFEFSRNALQERKLAQLPPSTFQALLRAES
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
DNATLAENFLSLCQQILQQICINNNSLDKLLILGPIPASMERRAGKYRFQLLLQSEQRAV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCEEEEEECCCCHHHH
ITQILHRALHAFDNLPEGRKVRWSIDVDPIDFV
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]