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The map label for this gene is ushA [H]

Identifier: 119946324

GI number: 119946324

Start: 3305298

End: 3307031

Strand: Direct

Name: ushA [H]

Synonym: Ping_2695

Alternate gene names: 119946324

Gene position: 3305298-3307031 (Clockwise)

Preceding gene: 119946313

Following gene: 119946326

Centisome position: 72.49

GC content: 43.54

Gene sequence:

>1734_bases
ATGAAAAAAATATTAATGAAAGGTTTAGTCGCAACAGCTGTGATTACAGCTTTAACGGCTTGTAATGACAATAACAGTGA
TCCACAGCTTGTTAGTGATCCAAAGCTTGTTGTTCCTCAAACCTGTGTTGAGGCTGCAGAGGACTGTACTACTTTTACTC
TGCTTCATACTAATGATAACCATGGCCGTTTCTGGGAAAATAAATACGGGGAATACGGCATGGCGGCACGTAAGACCTTG
ATCGATAATATTCGTGCGGAAGTTGAAGCTGATGGTGGAGAAAGTATTCTGCTCTCCGGCGGCGATATCAATACGGGGGT
GCCTGAATCTGATTTGCAGGATGCGATTCCAGATTTTATCGGTATGAACCTAATCGAATATGATGCTATGGCGGTCGGTA
ATCATGAATTTGATAACCCCTTATCTGTGGTAGAAATGCAGCGTAATTTGGCTAATTTTCCAATGCTGGCTGCCAATATA
TATGATAAAGCAACAGGTAAACGTTATTTTGATGCTTATAAAATATTCACTGTAAACGGACTGCGCATTGCTGTAATAGG
CTTAACGACTGAAGATACGGTTAAGATAGGCAATCCGGAATTTATTAGCAGTTTAGAGTTCAGGAATCCGCAAGAAGAAA
TTCAAAAAGTGATCAAAGAAATCAAAGCAGCCAATTCAGCCGATCTTATCTTTGCAACAACGCATATGGGGCATTATGCT
GATGGCCAAAACGGCAGCAATGCGCCGGGTGATGTTGCATTGGCGCGCGCTGTTAGCGAAGGTGACCTGCTGGCGATTAT
CGGCGGACATTCACAGAATCCCGTTTGTATGGAGCCCGGGGAAAATAGTTATGCCGCCGAATTTAAAGCGGGCGCGGATT
GTGCTCCAGATCAGCAAAACGGGACCTATATTCTGCAGGCACATGAATGGGGCAAGTATGTCGGTCGCGCTGATTTTGAA
TATTTCAACGGGAAATTACAACTGGCAAGTTACAAGTTAATTCCTGTTAACTTGAAAGAGAAAAATACAGAGGGTGAACG
TGTCTTGATCGAGGATGCGATTAAAGCTGATATAAATACCTATGAAGTACTCAAATATTATCAGGACAAAGGTCAGGAAT
CACTTGGCGAAGTGATTGCAAGCACAGCAGTAAAACTTGATGGGGATCGTTCAATTGTTCGTTTCAAGCAGACTAATCTA
GGTCATTTACTGGCAGAAGCACAAAGAGATAAAGTTGCTGCTGATATCGGAATAATGAACTCAGGTGGTGTGCGTGCTGA
TATTGAATTTGGAGATATCACCTATAAAGATGTATTGACTGTGCAGCCCTTTGCAAACACTGTTACCAAGAGCACCATGA
CAGGTGCACAGATCTCGACATACCTGGGTGAGGTTGCTTCTTTACAGATTGACTCCGGCGCTTATGCACAGTTAACCGGT
GTGCAAATGACAGTTAACTGTACGGCTAAGACGGTGGCTATCACAGATATTAATGGTAAAGGTTACGATGCGGCTGGATC
TTACAGCTTTACTGTGCCCAGCTTTAATGCCGCAGGCGGCGACGGATACCCCATCCTTACACCTGTTATGACTGGTTATG
TTGATGCTGAAGTACTGTACAGCTATCTGAAAGCACGGGTCTCTATTGCCGCTGGTGATTTTGCACCTTCAGCAAGTGAT
GTCATATATGAAAACTCGTTGAGTAGTGATGGTTGTCAGATAGCAGCTGGATAA

Upstream 100 bases:

>100_bases
ACAATTATAATGTATTCTTGAACTTGCAAATGGAAGAGCCAATGTTTCATTGCTACACGGAGACAGATTACTGATTAAGA
TAATTTAAGGGTTGATTGCA

Downstream 100 bases:

>100_bases
TACTTAAGTCATATTGAGAAAGGCTGGACGCCCAAACCTTTCTCTTATCCCCCTGGCCTCTTCCGGTGATTTTATTAAGA
GCTTATTACTGCTGTTTTCT

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKKILMKGLVATAVITALTACNDNNSDPQLVSDPKLVVPQTCVEAAEDCTTFTLLHTNDNHGRFWENKYGEYGMAARKTL
IDNIRAEVEADGGESILLSGGDINTGVPESDLQDAIPDFIGMNLIEYDAMAVGNHEFDNPLSVVEMQRNLANFPMLAANI
YDKATGKRYFDAYKIFTVNGLRIAVIGLTTEDTVKIGNPEFISSLEFRNPQEEIQKVIKEIKAANSADLIFATTHMGHYA
DGQNGSNAPGDVALARAVSEGDLLAIIGGHSQNPVCMEPGENSYAAEFKAGADCAPDQQNGTYILQAHEWGKYVGRADFE
YFNGKLQLASYKLIPVNLKEKNTEGERVLIEDAIKADINTYEVLKYYQDKGQESLGEVIASTAVKLDGDRSIVRFKQTNL
GHLLAEAQRDKVAADIGIMNSGGVRADIEFGDITYKDVLTVQPFANTVTKSTMTGAQISTYLGEVASLQIDSGAYAQLTG
VQMTVNCTAKTVAITDINGKGYDAAGSYSFTVPSFNAAGGDGYPILTPVMTGYVDAEVLYSYLKARVSIAAGDFAPSASD
VIYENSLSSDGCQIAAG

Sequences:

>Translated_577_residues
MKKILMKGLVATAVITALTACNDNNSDPQLVSDPKLVVPQTCVEAAEDCTTFTLLHTNDNHGRFWENKYGEYGMAARKTL
IDNIRAEVEADGGESILLSGGDINTGVPESDLQDAIPDFIGMNLIEYDAMAVGNHEFDNPLSVVEMQRNLANFPMLAANI
YDKATGKRYFDAYKIFTVNGLRIAVIGLTTEDTVKIGNPEFISSLEFRNPQEEIQKVIKEIKAANSADLIFATTHMGHYA
DGQNGSNAPGDVALARAVSEGDLLAIIGGHSQNPVCMEPGENSYAAEFKAGADCAPDQQNGTYILQAHEWGKYVGRADFE
YFNGKLQLASYKLIPVNLKEKNTEGERVLIEDAIKADINTYEVLKYYQDKGQESLGEVIASTAVKLDGDRSIVRFKQTNL
GHLLAEAQRDKVAADIGIMNSGGVRADIEFGDITYKDVLTVQPFANTVTKSTMTGAQISTYLGEVASLQIDSGAYAQLTG
VQMTVNCTAKTVAITDINGKGYDAAGSYSFTVPSFNAAGGDGYPILTPVMTGYVDAEVLYSYLKARVSIAAGDFAPSASD
VIYENSLSSDGCQIAAG
>Mature_577_residues
MKKILMKGLVATAVITALTACNDNNSDPQLVSDPKLVVPQTCVEAAEDCTTFTLLHTNDNHGRFWENKYGEYGMAARKTL
IDNIRAEVEADGGESILLSGGDINTGVPESDLQDAIPDFIGMNLIEYDAMAVGNHEFDNPLSVVEMQRNLANFPMLAANI
YDKATGKRYFDAYKIFTVNGLRIAVIGLTTEDTVKIGNPEFISSLEFRNPQEEIQKVIKEIKAANSADLIFATTHMGHYA
DGQNGSNAPGDVALARAVSEGDLLAIIGGHSQNPVCMEPGENSYAAEFKAGADCAPDQQNGTYILQAHEWGKYVGRADFE
YFNGKLQLASYKLIPVNLKEKNTEGERVLIEDAIKADINTYEVLKYYQDKGQESLGEVIASTAVKLDGDRSIVRFKQTNL
GHLLAEAQRDKVAADIGIMNSGGVRADIEFGDITYKDVLTVQPFANTVTKSTMTGAQISTYLGEVASLQIDSGAYAQLTG
VQMTVNCTAKTVAITDINGKGYDAAGSYSFTVPSFNAAGGDGYPILTPVMTGYVDAEVLYSYLKARVSIAAGDFAPSASD
VIYENSLSSDGCQIAAG

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=569, Percent_Identity=24.780316344464, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1786687, Length=566, Percent_Identity=50, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1790658, Length=469, Percent_Identity=21.9616204690832, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922446, Length=556, Percent_Identity=26.0791366906475, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24654424, Length=556, Percent_Identity=26.0791366906475, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI28573524, Length=531, Percent_Identity=24.4821092278719, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922444, Length=557, Percent_Identity=25.1346499102334, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24641187, Length=459, Percent_Identity=25.4901960784314, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI221329836, Length=392, Percent_Identity=22.4489795918367, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 62062; Mature: 62062

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILMKGLVATAVITALTACNDNNSDPQLVSDPKLVVPQTCVEAAEDCTTFTLLHTNDN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEECCHHHHHHHHCCEEEEEEEECCC
HGRFWENKYGEYGMAARKTLIDNIRAEVEADGGESILLSGGDINTGVPESDLQDAIPDFI
CCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GMNLIEYDAMAVGNHEFDNPLSVVEMQRNLANFPMLAANIYDKATGKRYFDAYKIFTVNG
CCCCEEECEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCEEEEEEEEEEECC
LRIAVIGLTTEDTVKIGNPEFISSLEFRNPQEEIQKVIKEIKAANSADLIFATTHMGHYA
EEEEEEEECCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
DGQNGSNAPGDVALARAVSEGDLLAIIGGHSQNPVCMEPGENSYAAEFKAGADCAPDQQN
CCCCCCCCCCCHHHHEECCCCCEEEEECCCCCCCEEECCCCCCEEEEECCCCCCCCCCCC
GTYILQAHEWGKYVGRADFEYFNGKLQLASYKLIPVNLKEKNTEGERVLIEDAIKADINT
CEEEEEECCCHHHCCCCCHHHCCCEEEEEEEEEEEEECCCCCCCCCEEEEEHHHHCCCCH
YEVLKYYQDKGQESLGEVIASTAVKLDGDRSIVRFKQTNLGHLLAEAQRDKVAADIGIMN
HHHHHHHHHCCHHHHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEC
SGGVRADIEFGDITYKDVLTVQPFANTVTKSTMTGAQISTYLGEVASLQIDSGAYAQLTG
CCCEEEEEEECCEEHHHEEEECCHHHHHHHHHCCCHHHHHHHCCHHEEEECCCCEEEEEE
VQMTVNCTAKTVAITDINGKGYDAAGSYSFTVPSFNAAGGDGYPILTPVMTGYVDAEVLY
EEEEEEECEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHHHCCCCHHHHH
SYLKARVSIAAGDFAPSASDVIYENSLSSDGCQIAAG
HHHHHHEEEEECCCCCCCCCEEEECCCCCCCCEEECC
>Mature Secondary Structure
MKKILMKGLVATAVITALTACNDNNSDPQLVSDPKLVVPQTCVEAAEDCTTFTLLHTNDN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCEECCHHHHHHHHCCEEEEEEEECCC
HGRFWENKYGEYGMAARKTLIDNIRAEVEADGGESILLSGGDINTGVPESDLQDAIPDFI
CCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GMNLIEYDAMAVGNHEFDNPLSVVEMQRNLANFPMLAANIYDKATGKRYFDAYKIFTVNG
CCCCEEECEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCEEEEEEEEEEECC
LRIAVIGLTTEDTVKIGNPEFISSLEFRNPQEEIQKVIKEIKAANSADLIFATTHMGHYA
EEEEEEEECCCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
DGQNGSNAPGDVALARAVSEGDLLAIIGGHSQNPVCMEPGENSYAAEFKAGADCAPDQQN
CCCCCCCCCCCHHHHEECCCCCEEEEECCCCCCCEEECCCCCCEEEEECCCCCCCCCCCC
GTYILQAHEWGKYVGRADFEYFNGKLQLASYKLIPVNLKEKNTEGERVLIEDAIKADINT
CEEEEEECCCHHHCCCCCHHHCCCEEEEEEEEEEEEECCCCCCCCCEEEEEHHHHCCCCH
YEVLKYYQDKGQESLGEVIASTAVKLDGDRSIVRFKQTNLGHLLAEAQRDKVAADIGIMN
HHHHHHHHHCCHHHHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEC
SGGVRADIEFGDITYKDVLTVQPFANTVTKSTMTGAQISTYLGEVASLQIDSGAYAQLTG
CCCEEEEEEECCEEHHHEEEECCHHHHHHHHHCCCHHHHHHHCCHHEEEECCCCEEEEEE
VQMTVNCTAKTVAITDINGKGYDAAGSYSFTVPSFNAAGGDGYPILTPVMTGYVDAEVLY
EEEEEEECEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCEEHHHHHHCCCCHHHHH
SYLKARVSIAAGDFAPSASDVIYENSLSSDGCQIAAG
HHHHHHEEEEECCCCCCCCCEEEECCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA